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    Evaluation of Dietary Protein Level in Practical Feed for Twoband Bream Diplodus vulgaris
    (Wiley, 2018) Yigit, Murat; Sahinyilmaz, Merve; Acar, Umit; Kesbic, Osman; Yilmaz, Sevdan; Bulut, Musa; Gurses, Kaan
    The effects of different dietary protein levels on growth performance and health status of TwobandBream Diplodus vulgaris juveniles were investigated. Three test diets that had increasing protein levels (30, 36, and 42%) and used fish meal as the protein source were formulated. Each of the test diets was fed in triplicate groups of 28 juvenile fish (initial weight, 23.65 +/- 0.34 [mean +/- SD]) to satiety for a period of 80d. Growth performance and feed utilization were best in the group fed the diet with a protein level of 36%. Ammonia nitrogen excretion showed an increasing trend as dietary protein levels increased, and retention rates of ammonia nitrogen per intake were highest in fish fed the 42% protein diet. Whole-body proximate composition and hematological variables were not significantly affected by protein level in the diets. However, serum glucose, aspartate aminotransferase (AST), alanine aminotransferase (ALT), and lactate dehydrogenase (LDH) values were significantly affected by dietary protein level and showed an increasing trend as the dietary protein level in the diets increased. As a result, an improved health status of Twoband Bream was observed in the fish fed a 36% protein level.
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    Öğe
    Temporal variations in hematological, immunological and serum biochemical parameters of rainbow trout (Oncorhynchus mykiss) acclimated to high-saline water in the Northern Aegean Sea
    (Walter De Gruyter Gmbh, 2023) Buyukates, Yesim; Kesbic, Osman; Yigit, Murat; Yilmaz, Sevdan; Ergun, Sebahattin; Bulut, Musa; Ozalp, Bans
    The present study evaluated the health status of rainbow trout (30.24 +/- 5.34 g) acclimated to high-saline water (28 parts per thousand). Among adaptation procedures, gradually-acclimated (for 48 h) fish showed best performance, which were then introduced to the offshore cage-farm for further monitoring of fish health in marine environment over a long-term period of 100 days, until harvest weight of 319.9 +/- 48.51 g. Fish health and welfare was evaluated by means of biochemical parameters (viscerasomatic index, hepatosomatic index, mesenteric fat index, and spleen somatic index), hematological parameters (serum glucose, triglyceride, cholesterol, total protein, albumin, globulin), and immune parameters (lysozyme, myeloperoxidase, respiratory burst and potential killing values). Gradual acclimation to high-saline water did not show any adverse effects on health parameters. No significant differences were found in biometric measures (P>0.05). However, a time-dependent increase was recorded in hematology markers (P<0.05) after seawater transfer, and the health status improved over long-term from March 7 to June 14, 2018. All serum biochemical markers, except the total protein showed significant alterations (P<0.05) in long-term but without detrimental influence by day-100. Therefore, it seems possible to expand trout farms from brackish water sites to higher saline environments up to 28 parts per thousand salinity, with no detrimental impacts on fish health, that in turns may significantly contribute to the extension of potential aquaculture sites to wider areas.