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Öğe A novel electrochemical biosensor based on carboxyethanephosphonic acid coated magnetic Fe3O4 nanoparticles for sensitive detection of resistin(Elsevier Inc., 2026) Aydın, Elif Burcu; Aydın, Muhammet; Kemal Sezgintürk, Mustafa KemalObesity has emerged as a huge public health issue around the world. Obesity raises the risk of certain chronic diseases, and the assessment of biomarkers indicative of the fundamental biological pathways associated with heightened disease risk may serve as an alternate method to delineate the pertinent obesity phenotype. In this context, we reported a potentially low-cost magneto-electrochemical biosensor built with carboxyl-functionalized magnetic nanoparticles (Fe3O4@SiO2@Cepa MNs)-attached indium tin oxide (ITO) electrode for the measurement of the resistin biomarker. A layer of anti-resistin antibodies was immobilized onto the active carboxyl ends of Fe3O4@SiO2@Cepa MNs via amide bonds, and the Fe3O4@@SiO2Cepa@anti-resistin MNs could detect the resistin obesity biomarker using electrical impedance spectroscopy (EIS). All the modification processes of the MNs were carried out in microcentrifuge tubes, and these MNs were magnetically captured onto the ITO surface for electrochemical measurements. Because of the specific interaction between the anti-resistin antibody and the resistin antigen, this system was highly sensitive and selective for resistin. A linear impedimetric response from 5 pg/mL to 750 pg/mL with a detection limit (LOD) of 1.48 pg/mL for resistin was achieved. The magneto-electrochemical biosensor displayed acceptable accuracy, good reproducibility, and high selectivity. Moreover, the high stability of the functionalized magnetic nanoparticles allowed the designed biosensing platform to obtain long-term stability. This magneto-electrochemical biosensor was successfully applied to the analysis of resistin in real samples, and the results of real samples detected by this system were in acceptable agreement with those measured by enzyme-linked immunosorbent assay (ELISA). Furthermore, high recovery rates in actual sample detection displayed that this magneto-electrochemical biosensor was a promising tool to be applied to the detection of resistin in clinical diagnosis. © 2024Öğe Advances in anti-drug antibody detection: Miniaturized biosensor technologies and beyond(Elsevier B.V., 2025) Uludağ Anıl, İnci; Kemal Sezgintürk, Mustafa KemalThe emergence of anti-drug antibodies (ADAs) poses a significant challenge in biological therapeutics, undermining drug efficacy and patient safety. This review thoroughly assesses existing and novel analytical methods for ADA detection, highlighting their principles, strengths, and limitations. Conventional techniques like ELISA and ECL provide great sensitivity but may be inadequate for detecting low-affinity ADAs. On the other hand, surface plasmon resonance (SPR) offers advantages in detecting low-affinity anti-drug antibodies (ADAs) due to its real-time kinetic assessment. Recent advancements in label-free technologies, such as thin-film interferometry, electrolyte-gated organic field-effect transistors, and quartz crystal microbalance, show significant potential for rapid, sensitive, and real-time ADA monitoring. These advanced platforms enable accurate kinetic characterization and offer promise for point-of-care applications. Additionally, novel approaches address limitations of conventional immunoassays by simplifying workflows and reducing assay time. This review underscores the importance of ADA assessment for optimizing personalized therapeutic strategies and improving patient outcomes. © 2025Öğe Conclusion and future prospects(Elsevier, 2025) Uludağ Anıl, İnci; Özcan, Burcu; Özyurt, Canan; Demirbakan, Burçak; Kemal Sezgintürk, Mustafa KemalLateral flow assays (LFAs) are regarded as a significant technique because of their extensive variety of applications. As discussed in this chapter, they are used in a variety of areas, including clinical diagnostics, environmental surveillance, food safety, hormone balance assessment, cancer biomarker identification, and toxin analysis. Although these tests are renowned for their ability to deliver quick and uncomplicated outcomes, they do have several drawbacks, including limited sensitivity and the need for multiple testing. Advancements in technology have provided substantial breakthroughs to address the constraints of LFAs. Recent advancements in nanoparticles, particularly gold nanoparticles, quantum dots, and magnetic nanoparticles, have greatly enhanced the ability to amplify signals and identify many targets. These nanoparticles enhance the sensitivity of LFAs, facilitating the identification of targets even at low concentrations. Furthermore, LFAs that utilize aptamers have the added benefits of increased stability and reduced costs as compared to systems that rely on antibodies. Aptamers can be readily customized with diverse functional groups, offering versatility in experimental design and optimization. Furthermore, the incorporation of surface-enhanced Raman spectroscopy technology enhances the sensitivity of LFAs, allowing for the detection of substances even at lower concentrations. Consequently, the advancement of technology has enhanced the sensitivity and enhanced the user-friendliness of these tests. © 2026 Elsevier Inc. All rights reserved..Öğe Introduction to lateral flow assays(Elsevier, 2025) Yence, Merve; Burcu Aydın, Elif; Aydın, Muhammet; Kemal Sezgintürk, Mustafa Kemal; Ozkan, Sibel A.; Caglayan, Mehmet GokhanLateral flow assays (LFAs) use paper-based devices for analyte detection and quantification in complex mixtures. LFAs have garnered attention for their user-friendly forms, quick test times, minimal interferences, affordable prices, and ease of operation by nonexperts. This introduction chapter presents an overview of the working principle of this technique, its advantages, applications in many fields, components of the method, and some critical parameters for developing LFAs. © 2026 Elsevier Inc. All rights reserved..Öğe Lateral Flow Assays(Elsevier, 2025) Ozkan, Sibel A.; Kemal Sezgintürk, Mustafa KemalLateral Flow Assays: From Bench to Market provides a detailed understanding of lateral flow assays (LFAs), the technology behind them, and their potential uses. The book opens with a discussion of the key elements of LFAs before covering a range of different LFA types, including aptamer-based and SERS-based LFAs. Applications across the biosciences and beyond are explored, such as virus detection, including Covid-19, bacteria detection, hormonal detection, and cancer biomarkers. LFAs for the detection of narcotics, environmental pollutants, food additives, allergens, toxins, and metabolites are also explored. The book then discusses commercialization aspects of LFAs before looking to future developments. This book offers a complete overview of these biosensors and is an ideal reference for researchers and lab technicians across the biomedical sciences who work with these devices. It is also relevant to researchers and practitioners in food chemistry, the environmental industry, and pharmaceutical companies, in addition to those involved in the development of lateral flow devices. © 2026 Elsevier Inc. All rights are reserved, including those for text and data mining, AI training, and similar technologies..Öğe Lateral flow assays for hormones(Elsevier, 2025) Cetinkaya, Ahmet; Kaya, S. Irem; Uludağ Anıl, İnci; Kemal Sezgintürk, Mustafa Kemal; Ozkan, Sibel A.Hormones are biological entities that are released by endocrine glands or tissues, and the critical metabolic and physiological pathways of the body require various hormones. Blood levels of hormones in an individual are important indicators of healthy or abnormal conditions. Accurate, rapid, and sensitive determination of the concentrations of hormones, which are usually at the picomolar-nanomolar level, is critical in health applications. In addition to this, it may also be possible to aim to increase efficiency and promote growth by adding steroid hormones to foods inappropriately. Detection of hormone residues in foods is also important to prevent situations that threaten food safety and human health. Lateral flow assays (LFAs) are advantageous, paper-based analysis platforms in which separation and selectivity features are integrated. LFAs are advantageous, paper-based analysis platforms in which separation and selectivity features are integrated. LFA design includes parts such as the bioreceptor, control zone, etc., and detection methods such as colorimetry or fluorescence can be used. In this chapter, the applications of LFAs in the health field for purposes such as diagnostics or monitoring of a condition and their use in food safety are examined. After giving information about the design of a standard LFA and the assay procedure, current studies containing the most important applications in hormone determination were evaluated in terms of target hormone, assay type, label, detection method used in LFA, linear range, limit of detection, examined sample, and recovery% value. While the current status of LFAs on a critical issue, such as hormone analysis, is discussed in detail, the future course is also evaluated. © 2026 Elsevier Inc. All rights reserved..Öğe Lateral flow assays for toxins, metabolites, and beyond(Elsevier, 2025) Aydın, Elif Burcu; Aydın, Muhammet; Kemal Sezgintürk, Mustafa Kemal; Ozkan, Sibel A.The most popular point-of-care biosensors for diagnostic, biomedical, food, agricultural, and environmental applications are lateral flow assays (LFAs). While the fundamentals of lateral flow assay technology date back several decades, newer nanomaterials have been employed as receptor attachment platforms or signal transducers, enhancing analytical capabilities. The recent development of LFAs illustrates their promising features, including their capacity to analyze several analytes and their speed, precision, dependability, and affordability for detecting toxins and metabolites. With their connection to smartphone technologies, they present an encouraging technological tool for point-of-care analysis, mobile medical services, and bioanalytical requirements. This chapter presents the LFAs for toxins and metabolites, investigates the construction protocol of LFAs, sensitivities and detection limits of LFAs, and limitations in complex sample applications. In addition, the challenges facing the next generation of LFAs and their commercialization are emphasized. Furthermore, this chapter will stimulate further study to create sophisticated, multifunctional, and integrated LFA for different applications. © 2026 Elsevier Inc. All rights reserved..Öğe Lateral flow assays in food analysis(Elsevier, 2025) Kaya, S. Irem; Cetinkaya, Ahmet; Kemal Sezgintürk, Mustafa Kemal; Ozkan, Sibel A.The intricacy of the global food systems has led to a focus on food safety and authentication, and food safety is a serious concern at every stage of manufacturing because foodborne microorganisms have a negative effect on human health. Furthermore, the presence of chemicals, drugs, heavy metals, microbial pathogens, mycotoxins, pesticides, and other potentially hazardous substances in food products poses a threat to food safety. Therefore, it is vital to develop a reliable and user-friendly tool to assess food quality. Traditional approaches to determining substances affecting seed safety in food analysis, like chromatographic techniques (high-performance liquid chromatography and gas chromatography), immunoassay techniques (enzyme-linked immunosorbent assay), polymerase chain reaction, mass spectrometry, and surface-enhanced Raman scattering are time-consuming, labor-intensive, and expensive, which makes them unsuitable for on-site use. The need for quick and easy methods for food safety has increased due to recurrent outbreaks of foodborne illness. Lateral flow assays (LFA) have gained popularity recently due to their on-site, low-cost, and quick pathogen detection capabilities. Here, recent advances in LFAs for detecting various food safety threats in food samples were examined, and the advantages and disadvantages of LFA performance were highlighted. In addition, the advantages and disadvantages of LFAs in food analysis, as well as future trends, will be discussed. The primary obstacles to the continued advancement of LFA platforms were explained, and LFAs could soon provide exceptional performance at the point of care that can compete with laboratory methods. © 2026 Elsevier Inc. All rights reserved..Öğe Lateral flow assays using aptamers(Elsevier, 2025) Özyurt, Canan; Demirbakan, Burçak; Özcan, Burcu; Uludağ Anıl, İnci; Kemal Sezgintürk, Mustafa Kemal; Ozkan, Sibel A.Aptamers, which are short single-stranded nucleic acids or peptides, have become versatile recognition elements as a result of their high specificity, stability, and cost-effectiveness. This chapter offers a thorough examination of aptamer-based lateral flow assays (LFA), with a particular emphasis on their principles, design strategies, and implications for environmental monitoring, food safety, and medical diagnostics. Aptamer-based systems provide superior chemical stability, an extended shelf life, and the ability to undergo a variety of modifications, in contrast to traditional antibody-based LFAs. This chapter also emphasizes the most recent developments in hybrid approaches that integrate aptamers with other biorecognition elements, signal amplification techniques, and aptamer selection methods. The novel concepts described in this chapter have the potential to facilitate the development of diagnostic instruments that are more cost-effective, portable, and efficient, and that have a broad range of applications in biosensor technology. © 2026 Elsevier Inc. All rights reserved..
