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    Çanakkale İli Koşullarında Yalova İncisi (Vitis vinifera L.) Üzüm Çeşidinden Yılda İki Ana Ürün Alma Olanaklarının Belirlenmesi
    (Çanakkale Onsekiz Mart Üniversitesi, 2024) Dardeniz, Alper; Şahin, Esra; Çoban, Harun; Kaya, Çağlar
    Bu araştırma, ‘ÇOMÜ Dardanos Yerleşkesi Ziraat Fakültesi Çiftçiliği Bitkisel Üretim ve Araştırma Birimi’ Sofralık Üzüm Çeşitleri Araştırma ve Uygulama Bağı’nda yer alan, 41B anacı üzerine aşılı 12 yaşlı ‘Yalova İncisi’ üzüm çeşidi üzerinde, iki vejetasyon yılı süresince yürütülmüştür. Çanakkale ili koşullarında ‘Yalova İncisi’ üzüm çeşidinden yılda iki ana ürün alma olanaklarının belirlenebilmesi amacıyla, üzüm çeşidi omcaları üzerinde farklı uygulamalar gerçekleştirilmiştir. Normal ürün (KNT) omcaları her iki yılda da herhangi bir uygulamaya tabi tutulmamıştır. Birinci ana ürün (BAÜ) uygulaması omcalarında ilk yıl bazı erkencilik uygulamaları gerçekleştirilmiş, bu erkencilik uygulamalarına ikinci yılda gerek duyulmamıştır. İkinci ana ürün (İAÜ) uygulaması omcaları erkencilik uygulamaları yapılmış olan aynı omcalardır. İAÜ hasadının hemen ardından mevcut yazlık sürgünler kış budamasında olduğu gibi 2–3 göz üzerinden yapraklı şekilde yeniden kısa budanmıştır. İlk yıl ve ikinci yılda en yüksek ortalama verim KNT’den (2161.8 g omca–1 ve 3372.6 g omca–1) alınmıştır. İlk yılda İAÜ uygulamasında en düşük (1229.9 g omca–1) ortalama verim elde edilirken, BAÜ uygulaması 1840.6 g omca–1 ile ara grubu oluşturmuştur. İkinci yılda İAÜ uygulamasından ancak 354.7 g omca–1 ortalama verim elde edilebilmiş, ortalama verim BAÜ uygulamasında da azalarak 1271.9 g omca–1’a gerileyerek başka bir grubu oluşturmuştur. Araştırma sonuçlarına göre; erkencilik uygulamaları yapıldığı takdirde, Çanakkale ili koşullarında ‘Yalova İncisi’ üzüm çeşidinden bir vejetasyon yılında iki ana ürün almanın mümkün olabileceği belirlenmiştir
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    Cardinal (Vitis vinifera L.) Üzüm Çeşidinde Taç Yönetimi Uygulamasının Omca Taç Mikroklimasına Etkilerinin Belirlenmesi
    (Çanakkale Onsekiz Mart Üniversitesi, 2024) Şahin, Esra; Çoban, Harun; Dardeniz, Alper; Kaya, Çağlar; Demir, Sefer; Avcı, Berna; Yanlıç, Tuğçe
    Bu araştırma, ‘ÇOMÜ Dardanos Yerleşkesi Ziraat Fakültesi Bitkisel Üretim Araştırma ve Uygulama Birimi’ ‘Sofralık Üzüm Çeşitleri Uygulama ve Araştırma Bağı’ndaki ‘Cardinal’ üzüm çeşidi üzerinde 2024 yılında yürütülmüştür. Araştırmada, kısa ve karışık kış budaması gerçekleştirilen omcalarda taç yönetimi uygulamasının omca taç mikroklimasına etkisinin belirlenmesi amaçlanmıştır. Elde edilen araştırma bulgularına göre; ‘Cardinal’ üzüm çeşidinde, budama ana etkisinin (BUAET) taç içi sıcaklığına ve taç içi bağıl neme etkisi istatistikî olarak önemli bulunmuştur. Karışık kış budamasında (KAKB) 34.79°C olan taç içi sıcaklığı kısa kış budamasında (KIKB) 35.13°C ile daha yüksek bir sonuç vermiştir. KAKB’nda %34.76 olan taç içi bağıl nem, KIKB’nda %34.04 ile daha düşük bir değer almıştır. Rüzgâr hızı açısından, KAKB ile KIKB arasında BUAET’nin herhangi önemli bir etkisi tespit edilememiştir. KAKB’nda 9560.2 lüks olarak belirlenen taç içi ışık şiddeti, KIKB’nda 10788.9 lüks olarak ölçülmüştür. Uygulama ana etkisinin (UYAET) sıcaklık, bağıl nem, rüzgâr hızı ve ışık şiddeti bakımından kontrol (KNT) ve taç yönetimi (TY) arasında istatistikî açıdan önemli farklılıklar meydana getirdiği belirlenmiştir. Buna göre omca taç içinde KNT’de 34.73°C olan sıcaklık, yapılan TY’nin ardından 35.19°C’ye yükselmiş ve bağıl nem ise %35.65’ten %33.15’e düşmüştür. Rüzgâr hızı 1.79 m sn–1’den 2.47 m sn–1’ye ve ışık şiddeti 7728.0 lüks’ten 12621.0 lüks değerine çıkmıştır. Bununla birlikte, salkım ve yaprak yüzey sıcaklığı değerleri de yapılan uygulamalardan istatistikî olarak etkilenmiştir. Sonuç olarak; incelenen omca taç içi mikroklima değerlerinin yapılan farklı uygulamalara göre istatistikî olarak önemli farklılıklar oluşturduğu, bu durumun da üzüm verimi, kalitesi, hastalıklar ve zararlı populasyonu gibi birçok faktör ile birlikte değerlendirilmesinin bağcılık açısından yararlı sonuçlar ortaya koyabileceği düşünülmektedir.
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    Comparative Biplot Analysis of Micropropagation of Viking Aronia Cultivar in Different Plant Tissue Culture Media
    (Aegean Agricultural Research Institute, 2024) Kaya, Çağlar; Sarıyer, Tolga
    In this study, the effect of different concentrations of some plant growth regulators on the in vitro micropropagation of the Viking aronia variety was investigated. In this context, 15 different culture media were prepared by adding plant growth regulators (BAP, IBA, GA3, TDZ) in various concentrations and combinations to the MS medium (Murashige and Skoog, 1962). Sterilised shoot tips were transferred to the relevant plant tissue culture media in three replications according to the randomized parcels trial pattern, with three explants in each replication. At the end of the four-week development period, average vitrification rate (VIR), average shoot length (SHL) and average number of nodes (NON) parameters were evaluated. Biplot analysis was employed to analyze the data obtained. According to the biplot analysis, B1I002G1T1: (1.0 mg L-1 BAP+0.02 mg L-1 IBA+1.0 mg L-1 GA3+1.0 mg L-1 TDZ), B1I002G1T05: (1.0 mg L-1 BAP+0.02 mg L-1 IBA+1 mg L-1 GA3+0.5 mg L-1 TDZ) and B1I002G05T1: (1.0 mg L-1 BAP + 0.02 mg L-1 IBA+0.5 mg L-1 GA3+1.0 mg L-1 TDZ) had a more positive effect on the number of nodes and shoot length. This study showed that the Viking aronia variety can be easily grown from cuttings by adjusting the plant growth regulator concentrations and combinations. Different media and plant growth regulators are needed for each aronia variety, so more studies are needed to improve success rates.
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    Current Approaches in Industrial Dairy Enzymes
    (Çanakkale Onsekiz Mart Üniversitesi, 2024) Kaya, Çağlar; Özatay, Şehnaz
    Enzymes are commonly used in the dairy industry to obtain high-quality milk products with desired characteristics and extended shelf life. This demand has led to the production of enzymes not only from animal sources but also from plant and microbial sources. The production of enzymes used in dairy products has gained significant commercial importance over time. Advances in DNA technology allow microorganisms to be used like factories to obtain desired enzymes in their most active forms, playing a critical role in improving product quality, optimizing production processes, and offering consumers various options. Moreover, expanding the application areas of enzyme technology in the dairy industry and continually seeking innovative solutions are crucial for sustainability and competitiveness in the sector. Future studies and research should focus on developing more efficient and environmentally friendly enzymes. This review discusses the purposes of enzyme use in the dairy industry, new technologies, and research and development topics
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    Determination of effective surface sterilization protocol in in vitro tissue culture for Giant Snowdrop (Galanthus elwesii Hook) bulbs
    (2023) Kaya, Çağlar; Sarıyer, Tolga; Şahin, Esra
    Giant Snowdrop (Galanthus elwesii Hook) is a species of snowdrop belonging to the Amaryllidaceae family. In this study, the deformation rates of the bulbs was calculated as % (percent). For sterilization G. elwesii bulbs were sterilized at different concentrations (1, 3, 5, 7, 9, 11 and 13 %) of sodium hypochlorite (NaOCl) for 5, 10, 15, 20, 25 minutes. The bulbs were rinsed with sterile distilled water 3 times for 5 minutes and then transferred to the MS medium. Contamination rates in MS nutrient medium were determined as percent (%) after 7 days. Deformation was not observed in the bulbs sterilized with 1 and 3% sodium hypochlorite solution however 100% contamination was detected. The most effective surface sterilization was obtained by soaking in 9-11% sodium hypochlorite solution for 5 and 10 minutes. As the sodium hypochlorite concentration and the application time increased, the surface sterilization of the bulbs increased, but the deformation rate of the scaly leaves of the bulbs increased due to this increase.
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    Determination of Genome Size Differentiation and Ploidy Levels in Some Citrus Rootstock Populations
    (Kahramanmaraş Sütçü İmam Üniversitesi, 2025) Polatöz, Sefa; Şeker, Murat; Kaya, Çağlar
    Determining the ploidy level of plant material used in breeding studies is of great importance, especially for biotechnology applications. The existing genetic diversity in citrus allows the use of rootstocks and varieties that can be adapted to different climatic and soil conditions through different breeding methods. Several suitable rootstocks are used for commercial citrus. This study was conducted to determine the genome size and ploidy levels of citrus rootstocks widely used worldwide using flow cytometry. The rootstocks used in the study included Gou-Tou, C-35, Troyer citrus, Taiwanica, Citremon, Yuzu, Sunki mandarin, Flying Dragon, Yuma citrus, Macrophylla and Chinese orange. Fresh leaf tissue from each rootstock was mixed with triploid Tahitian lemon leaf tissue, used as a standard species, and cell nuclei were isolated. The cells stained with propidium iodide were analysed by flow cytometry, and histograms and cytograms were obtained. According to the results, although all species had diploid genome sizes, differences were observed between species in terms of genome volume. Yuzu seedlings were found to have the largest genome size (0.808 pg/2C), while Flying Dragon trifoliate had the smallest genome size (0.700 pg/2C).
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    Determination of the Effects of Different DNA Isolation Methods on Quantity and Quality
    (2025) Kaya, Çağlar; Şahin, Esra
    Objective: This study compared the effects of different DNA extraction methods (CTAB, phenol-chloroform, silica columns, magnetic beads) on the DNA yield and purity obtained from maize (Zea mays L.), bean (P. vulgaris L.), oak (Q. robur L.) and scots pine (P. sylvestris L.). Materials and Methods: In this study, young apical leaves (the most distal fully expanded leaves) of maize, bean, oak and scots pine were used. For each species, 10 leaf tissue samples were collected from young apical leaves during the vegetation season of the year 2024 from plantations located at different sites within the Bayramiç district of Çanakkale province. Leaf tissue samples were collected during the vegetation season of the year 2024, specifically during the early phenological stages of each species. For maize and bean, samples were obtained in the early vegetative growth phase, when the apical leaves were fully expanded but still young and metabolically active. For oak and Scots pine, sampling was conducted during the early spring, shortly after bud burst, when newly emerged apical leaves were in the initial phase of leaf expansion. Young apical leaves were preferred as they contain fewer secondary metabolites and phenolic compounds, which can interfere with DNA extraction and downstream molecular applications. This sampling strategy ensured the collection of high-quality tissue suitable for genomic DNA isolation. DNA isolation was performed using four different methods: CTAB, phenol-chloroform, silica-based columns, and magnetic beads. These methods were compared in terms of their suitability for genetic analyses specific to each plant species. Results: Using the phenol-chloroform method, a notably high DNA yield was obtained from P. sylvestris L. samples (371.75 ng/µL). Similarly, Q. robur L. also exhibited high yield (352.00 ng/µL), suggesting that these species are particularly compatible with this method. The CTAB method also yielded successful results. P. sylvestris L. achieved a high DNA yield (323.75 ng/µL), indicating that this species' genetic material is compatible with the CTAB method. A DNA yield of 308.00 ng/µL was observed for Q. robur L., which may necessitate additional steps for protein removal. The silica column method yielded DNA samples of a lower quantity than the other three methods. For P. sylvestris L. and Q. robur L., yields of (230.75 ng/µL) and (222.25 ng/µL), respectively, were recorded. Lower yields were observed in P. vulgaris L. (171.75 ng/µL) compared to Zea mays L. (211.75 ng/µL). The diminished DNA recovery may be attributable to the constrained binding capacity of silica columns or inadequate initial material. In the magnetic beads method, DNA yields for P. sylvestris L. (206.25 ng/µL), Q. robur L. (201.75 ng/µL), Zea mays L. (173.50 ng/µL) and P. vulgaris L. (151.75 ng/µL) were the lowest among all methods. Conclusion: This study compares DNA isolation methods including CTAB, phenol-chloroform, silica columns, and magnetic beads for Zea mays L., P. vulgaris L., Q. robur L., and P. sylvestris L. The findings reveal that the differences in DNA yields and purity depend on the plant species and tissue characteristics. The phenol-chloroform method provided the highest DNA yields and purity across all plant species. This method excels in obtaining DNA free from proteins, polysaccharides, and phenolic compounds, ensuring high molecular integrity. The results demonstrate that Q. robur L. and P. sylvestris L., which are rich in lignin and phenolic compounds, had the highest yields and preserved molecular integrity. This supports the use of the phenol-chloroform method in molecular analyses requiring high-quality DNA.
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    Determination of the Effects of Different Tree Logs on Yield and Some Quality Properties in Oyster Mushroom (Pleurotus ostreatus (Jacq.) P. Kumm.)
    (Bingöl Üniversitesi, 2023) Sarıyer, Tolga; Kaya, Çağlar; Gündoğdu, Mehmet Ali; Şahin, Esra; Çiftci, Hatice Nihan; Kuzucu, Fatih Cem; Alkan, Yavuz
    Oyster mushroom (Pleurotus spp.), which is the second most cultivated mushroom species in the world after the cultivated mushroom (Agaricus bisporus), can be grown at lower temperatures than other species and needs less specific ecological factors, making it easier to cultivate. In the current study, oyster mushroom (Pleurotus ostreatus) was grown in substrates prepared by using logs of beech (Fagus spp.), chestnut (Quercus spp.), poplar (Populus spp.), and linden (Tilia spp.) trees. In this context, the study was carried out in the mushroom production room of Canakkale Onsekiz Mart University, Faculty of Agriculture, Department of Horticulture. In the study, yield, fruit characteristics, protein amount, soluble solids, pH, TETA (Titratable acidity) and flavor value were determined. It was observed that Pleurotus ostreatus micelles did not spread on the linden logs during the incubation stage. When other logs were evaluated, it was determined that beech, poplar and chestnut logs were more suitable for oyster mushroom cultivation in terms of yield, respectively. While the lowest protein value was obtained in the beech log substrate, the highest protein value was obtained in the chestnut log substrate. In terms of external appearance, it can be said that beech and poplar logs have similar and more suitable values. In terms of the study results, beech medium can be recommended for cultivation with the aim of high
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    Determination of the Effects of Gradual Canopy Management Applications on Grapevine Microclimate in Yalova Incisi (Vitis vinifera L.) Grape Variety
    (Mevlüt AKÇURA, 2023) Şahin, Esra; Dardeniz, Alper; Çoban, Harun; Kaya, Çağlar
    This research was carried out in the ‘Table Grape Varieties Application and Research Vineyard’ in the ‘Plant Production Research and Application Unit of COMU Dardanos Campus, Faculty of Agriculture’, in 2019 and 2021. In the research, it was aimed to determination of the effects of gradual canopy management applications on grapevine microclimate in 'Yalova İncisi' grape variety. The first situations of grapevine before the canopy management applications constituted the control (CNT) application. After that, gradual canopy management applications on the same grapevines were carried out at the EL–17 phenological stage. In this context; removal of water sprouts (RWS), removal of lower bottom leaves and lower axillary shoots (RLBL+RLAS) and removal of upper axillary shoots and topping (RUAS+TP) were performed on the same grapevines, respectively. Temperature (°C), relative humidity (%), amount of light (Lux) and wind speed (m sec–1) parameters were investigated in terms of the effects of gradual canopy management applications on the microclimate inside and outside the canopy of the grapevine. All applications and measurements were carried out between 11:00 and 15:00 of the day. According to the two–year research findings; in terms of temperature values, no significant difference was detected between the applications inside and outside the canopy, but numerical increases were determined from control to gradual canopy management. In terms of relative humidity values, no significant difference was detected inside and outside the canopy, but regular numerical decreases were recorded from control to gradual canopy management. While no significant difference could be detected between the applications outside the canopy in terms of the amount of light, the application of RUAS+TP (7541 Lux) reached the highest amount of light inside the canopy. With the gradual implementation of canopy management practices in grapevines, significant regular increases in the amount of light inside the canopy have occurred. The lowest wind speed in the canopy was determined in CNT (1.39 m sec–1), the highest wind speed was determined in RUAS+TP (1.89 m sec–1) and RLBL+RLAS (1.83 m sec–1) applications, respectively. The effects of canopy management practices according to the gradual were also reflected numerically on the microclimate outside the canopy
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    Development of an Effective Sterilization Protocol for Plant Tissue Culture Studies in Superfruit Aronia [Aronia melanocarpa (Michaux) Elliot]
    (Karyay Karadeniz Yayımcılık, 2024) Kaya, Çağlar; Özatay, Şehnaz
    Effective sterilization protocols are crucial for a successful tissue culture study in Aronia. These protocols directly influence contamination rates, shoot health, and root development. In this context, the study aims is to develop an effective sterilization protocol for plant tissue culture studies in Aronia [Aronia melanocarpa (Michaux) Elliot], commonly known as the "superfruit." In the study, the Nero Aronia variety shoot tips were used as material. The sterilized shoot tips were transferred to the respective plant tissue culture media in a randomized parcels trial pattern with three replicates, each containing three explants per replicate. Various concentrations and combinations of sterilizing agents, such as sodium hypochlorite (NaOCl), hydrogen peroxide (H2O2), mercuric chloride (HgCl2), and ethanol (C2H5OH), were evaluated to determine their effectiveness in maintaining tissue health and reducing contamination. Twelve protocols were developed, incorporating different concentrations of these chemicals. The data were subjected to statistical analysis using the SAS software package (LSD0.05-Proc GLM). Analysis of variance (ANOVA) was performed to evaluate differences among sterilization treatments for each parameter. The results showed that the combination of 5% NaOCl and 3% H2O2 (10 min each) provided the lowest average contamination rate 0.0%, the highest average number uncontaminated explants 9.0 pieces, shoot length 3.0 cm and root length 2.5 cm demonstrating the sterilization efficiency of this combination. On the other hand, protocols containing HgCl2, especially at higher concentrations, resulted in impaired root development. High ethanol concentrations also contributed to effective sterilization, with the combination of 7% NaOCl (10 min) and 80% ethanol (5 min) yielding a low contamination rate (22.0%) and preserving tissue health. This study emphasizes balancing sterilization protocols between effective contamination control and tissue viability. The findings are expected to benefit the improvement and development of tissue culture techniques for Aronia and similar species, providing a basis for further research on effective sterilization practices, currently limited in Aronia tissue culture.
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    Effects of Some Priming Applications on Quality Parameters of Melon (Cucumis melo L.) Seeds Under KNO3 Stress
    (Bingöl Üniversitesi, 2022) Kaya, Çağlar; Sarıyer, Tolga; Şahin, Esra
    Adverse environmental conditions reduce seed germination and prolong germination period. P Pre-applications of the seeds to increase the germination are important. Some of the priming applications increasing germination are the process of keeping the seeds in pure water and sage extract for a certain period of time. This study conducted to determine the effects of priming and KNO3 applications on some seed quality parameters in melon obtained from melon producers in Çanakkale region. Melon seeds were subjected to control (no priming and KNO3), different priming (pure water, sage) and salt stress (KNO3) treatments. As a result of the measurements made on the average germination time, average germination rate, average hypocotyl length, average radicle length and average radicle thickness parameters in the study, it was observed that the quality parameters that showed good improvement with priming applications decreased with KNO3 stress. Priming of both sage and distilled water significantly increased the mean germination rate under KNO3 stress. The most positive effect was the sage application, the least effect was the control (no priming) application
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    Effects of Some Priming Applications on Quality Parameters of Melon(Cucumis melo L.) Seeds Under KNO3 Stress
    (Bingol University Bingöl Üniversitesi, 2022) Kaya, Çağlar; Sarıyer, Tolga; Şahin, Esra
    Adverse environmental conditions reduce seed germination and prolong germination period. P Pre-applications of the seeds to increase the germination are important. Some of the priming applications increasing germination are the process of keeping the seeds in pure water and sage extract for a certain period of time. This study conducted to determine the effects of priming and KNO3 applications on some seed quality parameters in melon obtained from melon producers in Çanakkale region. Melon seeds were subjected to control (no priming and KNO3), different priming (pure water, sage) and salt stress (KNO3) treatments. As a result of the measurements made on the average germination time, average germination rate, average hypocotyl length, average radicle length and average radicle thickness parameters in the study, it was observed that the quality parameters that showed good improvement with priming applications decreased with KNO3 stress. Priming of both sage and distilled water significantly increased the mean germination rate under KNO3 stress. The most positive effect was the sage application, the least effect was the control (no priming) application. Olumsuz çevre koşulları tohumlarda çimlenmeyi azaltmakta ve çimlenme süresinin uzamasına sebep olmaktadır. Bu nedenle tohumlarda çimlenmeyi artırmaya yönelik ön uygulamalar önem teşkil etmektedir. Tohumlarda çimlenmeyi artıran priming uygulamalarından bazıları, tohumların saf su ve adaçayı ekstraktında belirli bir süre bekletilmesi işlemidir. Bu çalışma, Çanakkale bölgesindeki kavun üreticilerinden temin edilen kavun tohumlarında priming ve KNO3 stres uygulamalarının bazı tohum kalite parametreleri üzerine etkilerini belirlemek amacıyla yapılmıştır. Kavun tohumları kontrol (priming ve KNO3 uygulanmayan), farklı priming (saf su ve adaçayı) ve tuz stresi (KNO3) uygulamalarına tabi tutulmuştur. Çalışmada ortalama çimlenme süresi, ortalama çimlenme hızı, ortalama hipokotil uzunluğu, ortalama radikula uzunluğu ve ortalama radikula kalınlığı parametreleri üzerinde yapılan ölçümler sonucunda, priming uygulamaları ile iyi gelişme gösteren kalite parametrelerinin KNO3 stresi ile azaldığı gözlemlenmiştir. Özellikle hem adaçayı hem de saf su priming uygulamaları, KNO3 stresi altında ortalama çimlenme oranını önemli ölçüde artırmıştır. En olumlu etki adaçayı uygulaması, en az etkiyi ise kontrol (priming yapılmayan) uygulaması göstermiştir.
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    Examination of Department Preference Perceptions of Students Who Win the Food Processing Department: The Example of Ezine Vocational School
    (Çanakkale Onsekiz Mart Üniversitesi, 2024) Kaya, Çağlar; Özatay, Şehnaz
    Vocational schools play a pivotal role in meeting the demand for intermediate staff in our country. Recently, programmes in the Food Processing Department have gained popularity. The Covid-19 pandemic has emphasised the significance of food science and technology, as well as agricultural activities, in increasing food supply. This study aims to identify the factors that influence the preferences, perceptions, and decision-making processes of newly enrolled students in the Food Processing Department programmes at Ezine Vocational School. The study analysed data from a survey of 50 students enrolled in EMYO’s Food Quality Control and Analysis and Dairy and Products Technology programmes. The survey consisted of 11 questions and the data was evaluated using Microsoft Excel. The results indicated that over half of the students were female, with the majority hailing from provinces in the Marmara Region. It was observed that the majority of students in the Food Processing Department had graduated from Vocational High School or Anatolian High School. Additionally, 20% of them had chosen the department due to their positive perception of the city of Çanakkale. Most students had selected the Food Processing Department with a clear awareness of the good job opportunities it offers
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    Farklı tef [Eragrostis tef (Zucc.) Trotter] genotipleri arasındaki genetik ilişkinin moleküler yöntemlerle belirlenmesi
    (Çanakkale Onsekiz Mart Üniversitesi, 2021) Kaya, Çağlar; Tiryaki, İskender
    Son zamanlarda tüm dünyada dengeli beslenmenin alternatif bitkilerinden biri olarak gösterilen tef [Eragrostis tef (Zucc.) Trotter] birçok biyotik ve abiyotik stres koşulları altında yetişebilen önemli bir buğdaygil bitkisidir. Bu çalışma 19 tanesi Amerika Birleşik Devletleri Gen Bankası ve 1 tanesi TR-Ticari çeşit olan tef genotiplerinin morfolojik ve moleküler karakterizasyonunu yapmak amacıyla yürütülmüştür. Genotiplere ait moleküler karakterizasyon çalışmaları 10 adet SSR DNA markırları kullanılarak gerçekleştirilmiştir. Bu markırlar toplamda 18 adet bant üretmiş olup bu bantların 12 tanesinin polimorfik olduğu belirlenmiştir. Markırlara ait ortalama polimorfizm oranı %66,6 olarak hesaplanmıştır. En yüksek polimorfizm bilgi içeriği (PIC), 0,69 ile CNLTs370 markırından elde edilmiştir. NTSYs programı ile DICE yöntemine göre oluşturulan matriks kullanılarak UPGMA yöntemine göre oluşturulan dendogramda genetik olarak birbirine en yakın genotiplerin 1,0 benzerlik kat sayısıyla (PI 193508, PI 194926, PI 195933–2), (PI 193510, PI 193514–2), (PI 193513, PI 193514–1, PI 194924–2, PI 195933–1), (PI 195938, PI 197210, TR-Ticari), (PI 193971–2, PI 194924–1), (PI 194923–1, PI 194923–2) ve (PI 194925, PI 195932) genotipleri arasında olduğu belirlenmiştir. Flow sitometri analiz sonuçları, ilgili tef genotiplerinin ortalama çekirdek DNA miktarının (2C) 1,46 pg olduğunu göstermiştir. Çalışma sonuçları, introdüksiyon materyali olarak temin edilen tef genotipleri arasında incelenen lokuslar bakımından DNA seviyesinde farklılıkların var olduğunu, çalışmada kullanılan tef bitkisine özgü SSR DNA markırlarının bu farklılıkların tespitinde ve genotipler arasındaki genetik ilişkinin ortaya konulmasında başarılı bir şekilde kullanılabileceğini ve var olan genetik varyasyonun ıslah çalışmalarında kullanılabileceğini göstermiştir. Anahtar sözcükler: Tef [Eragrostis tef (Zucc.) Trotter], DNA, SSR, Ploidy, Flow sitometri
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    Kurfalı Yerel Bamya Genotipinde Farklı Gübreleme Uygulamalarının Meyve Kalite Parametrelerine Etkileri
    (Atatürk Bahçe Kültürleri Merkez Araştırma Enstitüsü, 2024) Sarıyer, Tolga; Demir, Sefer; Kaya, Çağlar; Cankı, Fatih Furkan
    Bamya (Abelmoschus esculentus), Türkiye’de önemli miktarlarda yetiştiriciliğinin yanı sıra bazı yörelerimizde düğün, bazı bayramlar gibi önemli günlerde tüketilmesi yönüyle kültürel değeri de olan bir sebzedir. Günümüzde verimi ve kaliteyi arttırmak adına gereken miktardan yüksek dozlarda inorganik gübreleme sonucunda çevre ve sağlık sorunları ortaya çıkmaktadır. Son yıllarda mikrobiyal gübreler ve vermikompost gibi organik gübreler ile topraklara organik yapısının geri kazandırılmasına yönelik çalışmalar yapılmaktadır. Çalışmada Balıkesir Gömeç yöresinde yoğun olarak yetiştirilen Kurfalı yerel bamya genotipi Çanakkale koşullarında yetiştirilmiştir. Çalışma konuları gereken dozda inorganik gübre, gereken inorganik gübre ile birlikte mikrobiyal gübre (EM) ve gereken inorganik gübre ile birlikte sıvı vermikompost gübresi olarak belirlenmiştir. Çalışmada her tekerrürden seçilen bitkilerden bitki başı beş meyve tomurcuktan itibaren işaretlenerek beş gün sonunda hasat edilmiş ve kalite özellikleri (meyve ağırlığı (g), meyve eni (cm), meyve boyu (cm), renk (L, a, b), yaş-kuru ağırlık oranı (%), toplam fenolik bileşik miktarı (mg GAE/100 g) belirlenmiştir. Çalışma sonucunda organik gübreleme uygulamaları ile meyve ağırlığının arttığı ve bazı parametrelerin organik gübre türüne göre önemli farklılıklar gösterdiği gözlenmiştir. Ayrıca, Kurfalı bamya genotipinin Çanakkale yöresinde daha erken hasada gelmesi bu yörede de yetiştirilmesinin avantajlı olabileceğini göstermiştir.
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    Near Future Economic Effects of Ezine Food Specialized Organized Industrial on Çanakkale Region
    (Çanakkale Onsekiz Mart Üniversitesi, 2024) Özatay, Şehnaz; Kaya, Çağlar
    Food specialized organized industrial zone is an industrial zone specifically designed and equipped to cater to businesses operating in the food sector. These zones are established in Turkey to meet the needs of businesses engaged in activities such as food production, processing, packaging, storage, and distribution. The economic impacts of food specialized organized industrial zones are being widely assessed. These zones contribute to economic development by increasing employment opportunities and facilitating job placement for the local population. Furthermore, they encourage technological advancements, support R&D activities, and enhance productivity within the sector. By focusing on export-oriented production, these zones boost foreign trade volumes and add value to the national economy. This study aims to analyze and evaluate the potential future economic effects of the Ezine food specialized organized industrial zone in Çanakkale region. This review paper examines the current status and development plans of the industrial zone, identifies the key economic sectors that will be impacted, and explores the potential benefits and challenges that may arise as a result. The findings contribute to a comprehensive understanding of the future economic landscape of the region and provide insights for policymakers, investors, and local communities.
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    Örtü Bitkisi (Cover Crops) Uygulamasının Catherina Şeftali Çeşidinin Verim, Fenolojik Özellikler, Pomolojik Özellikler ve Meyve Uçucu Bileşenleri Üzerine Etkilerinin Belirlenmesi
    (2024) Şeker, Murat; Efil, Levent; Özer, Emin; Gür, Engin; Ekinci, Neslihan; Gündoğdu, Mehmet Ali; Kaya, Çağlar; Cankı, Fatih Furkan; Demirci, Sefa
    Örtü bitkilerinin yaygın olarak bilinen olumlu etkileri arasında toprak verimliliği ve meyve ağaçlarında kılcal kök faaliyetlerinin arttırılması, dolayısıyla mikrobesin elementlerinden daha yüksek seviyede yararlanma, hastalık ve zararlı mücadelesinde çok yönlü kazanımların elde edilmesi, etkin bir yabancı ot kontrolünün sağlanması ve toprak pH düzenlemesi bulunmaktadır. Canlı malç olarak da bilinen örtü bitkisi uygulamalarının meyve verim ve kalitesi üzerine de olumlu etkileri bulunmaktadır. 2021 2022 yıllarında yürütülen bu çalışma, örtü bitkisi uygulamasının Catherina şeftali çeşidinin verim, fenolojik özellikler, pomolojik özellikler ve meyve uçucu bileşenleri üzerine etkilerinin belirlenmesi amacıyla yürütülmüştür. Anadolu Etap Kumkale işletmesinde (Çanakkale) bulunan deneme parselinde belirlenen sıra aralarına 9 farklı türe ait tohum karışımı ekilmiş ve tohumların çimlenmesini takiben arazi koşullarında fenolojik, fizyolojik ve vejetatif gelişme performansını belirlemeye yönelik ölçümler yapılmış, meyve verimini belirlemek için sayımlar gerçekleştirilmiş, meyve örnekleri alınarak kalite analizleri ile olgun meyvelerde uçucu aroma bileşenlerini belirlemeye yönelik kromatografik analizler yapılmıştır. Örtü bitkilerinin şeftalilerde meyve fiziksel özellikleri ile biyokimyası üzerine değişik etkileri olduğu sonucuna ulaşılmıştır. Örtü bitkisi uygulamasının meyvelerde lakton, ester ve bazı önemli terpen bileşiklerinin sentezini yoğunlaştırdığı belirlenmiştir.
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    The effect of keeping clusters on vines on berry quality and biochemical properties of Müşküle (V. vinifera L.) grape variety
    (Harran Üniversitesi, 2025) Dardeniz, Alper; Şahin, Esra; Çoban, Harun; Kaya, Çağlar
    This research was conducted in the 'Table Grape Varieties Application and Research Vineyard' located in the ‘ÇOMÜ Dardanos Campus, Faculty of Agriculture, Plant Production Research and Application Unit’in 2020 and 2021. The aim was to determine the effects of keeping the clusters on the vine in the Müşküle (V. vinifera L.) grape variety on berry quality and biochemical properties. In this context, sampling was carried out in one-week periods for six weeks, starting from harvest maturity (EL–38; 23.10.2020–13.10.2021). According to the two–year findings; no significant change was detected between weeks in berry width, berry length and pH values. Berry weight followed a slightly fluctuating course but reached the highest value in the 6th week (4.35 g berry–1), and skin thickness decreased from 0.250 mm berry–1 to 0.180 and 0.160 mm berry–1 in the 6th and 5th weeks. It was determined that as the weeks progressed, the skin color of the berries changed from greenish–yellow to more yellow tones due to ripening, and the brightness and color vibrancy decreased. The TSS value increased by approximately 0.50% in the 6th week compared to the first weeks, the acidity value gradually decreased as the weeks progressed, and the maturity index gradually increased from 51 to 56. It was determined that total phenolic compound and tannin amounts decreased as maturity progressed, and the lowest values were determined to be 3.09 mg GAE 100 ml–1 and 0.557 mg kg–1 in the 6th week, respectively. This research reveals that in early autumn years when frosts are not seen in ecologies such as Çanakkale, keeping grape clusters on vines so that they can be offered for sale during a period when their marketing values are high will provide economic advantages
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    The Effects of Some Priming Applications on Seed Quality Parameters in Melon (Cucumis melo L.) Seeds Under Different Doses of NaCl Stress
    (2022) Sarıyer, Tolga; Kaya, Çağlar
    Salinity of irrigation water and soil-based salinity is an important problem. Priming is an application that increases the germination rate by keeping the seeds in distilled water or different aqueous solutions for a certain period of time. Ipsala variety melon (Cucumis melo L.) seeds obtained from Manisa Kırkağaç region were used in the study to determine the effects of different NaCl stress and priming applications on seed quality. Melon seeds subjected to different priming applications were germinated in pure water (0 mM NaCl) and salt stress (140 mM NaCl) conditions. In the study, herbal origin thyme (Origanum vulgare L. subsp. hirtum), sage (Salvia officinalis L.) applications as well as KNO3 (50 mM) and pure water applications were included as priming applications. In the study, it was determined that increase in the germination rate (%) and germination time (day) parameters which are important quality parameters in seeds, were more pronounced with priming applications. Priming with using sage and thyme plants were more effective respectively in increasing seed quality parameters.
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    The Pomological and Morphological Characterization of Different Foreign Almond Cultivars and the Local Type Grown under Ecological Conditions of Çanakkale
    (2025) Kaya, Çağlar; Seker, Murat
    The present study aims to investigate the pomological and morphological characteristics of different foreign almond [Prunus dulcis (Miller) D.A. Webb] cultivars and local type cultivated in Çanakkale ecological conditions. Within the scope of the study, Ferragnes, Ferraduel, Texas, Laurenne and Nonpareil were used as foreing almond cultivars, while Alçıtepe was selected as local type. Two-year data were used in the study. In 2023 and 2024, fruit characteristics of the cultivars and local type harvested in August-September, shelled and ushelled measurements, leaf and petiole characteristics and SPAD values indicating photosynthetic capacity were compared to obtain in-depth information about the production potential and quality parameters of different cultivars and local type. With regard to shelled and unshelled characteristics, the Ferraduel cultivar exhibited the longest shell width (23.32 mm), the Nonpareil cultivar demonstrated the longest shell length (38.99 mm), while the Ferragnes and Texas cultivars exhibited shorter shell characteristics. The highest unshelled fruit weight was found in cultivar Laurenne (2.46 g), while local type Alçıtepe had the lowest unshelled weight (1.30 g). Regarding the unshelled yield ratio, the cultivars Texas (40.31%) and Ferragnes (33.23%) had the highest yield ratios. These results show that there is a large variation in shelled yield ratio between cultivars. When leaf and petiole characteristics were analysed, Ferraduel cultivar had the largest leaf (32.80 mm), while Alçıtepe local type and Texas cultivar had smaller leaves. In terms of SPAD value, Laurenne (42.16) and Alçıtepe (41.60) had the highest values, while Texas (37.38) and Ferragnes (38.56) had lower SPAD values. This shows that there are significant differences in the photosynthetic capacity of different cultivars and this is an important factor in terms of productivity. In conclusion, this study revealed significant differences in pomological and morphological characteristics of some important almond cultivars and local type and highlighted that some cultivars provide larger fruits and higher yields. It is predicted that the results obtained will be an important guide for the selection of productive cultivars in almond production and the improvement of production techniques for almond local type. Keywords: Local type, standart cultivars, pomological traits, morphological traits.
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