Yazar "Karagoz, Halit" seçeneğine göre listele

Listeleniyor 1 - 3 / 3
  • [ X ]
    Öğe
    Analysis of genetic variation and population structure among of oregano (Origanum acutidens L.) accessions revealed by agro-morphological traits, oil constituents and retrotransposon-based inter-primer binding sites (iPBS) markers
    (Springer, 2020) Karagoz, Halit; Cakmakci, Ramazan; Hosseinpour, Arash; Ozkan, Gutter; Haliloglu, Kamil
    The genetic associations among 31 oregano (Origanum acutidens (Hand.-Mazz.) Ietswaart) genotypes collected from different provinces of Turkey were determined by comparing their agro-morphological traits, essential oil components and molecular traits. In order to assess the genetic diversity in 9 agro-morphological traits and 9 essential oil components, 10 polymorphic iPBS primers were used. Principle component analysis (PCA) for agro-morphological traits had two axes explaining 81.35% of total variation and PCA for essential oil components had 4 axes explaining 89.67% of total variation. Cluster analysis yielded two main clusters for agro-morphological traits and four main clusters for essential oil components. Present iPBS primers generated 289 alleles. Number of alleles per polymorphic locus varied between 25 and 36 with an average value of 28.40. The average polymorphism ratio was as 98.36%. Polymorphic information content values varied between 0.28 and 0.42. The average number of alleles (ne), Nei's genetic diversity (h) and Shannon's information index (I) were obtained 1.61, 0.37 and 0.55, respectively. The 31 accessions were assembled into three main clusters according to the unweighted pair-group mean average based on iPBS markers. Similarity index value varied between 0.51 and 0.81. Moreover, population structure analysis using Bayesian method showed that all genotypes formed eight sub-population (K = 8) with an average value of 0.3544 for expected heterozygosity and 0.0400 for population differentiation measurements (F-st value). In present study, population structure of 31 oregano genotypes collected from different provinces of Turkey were successfully characterized with the aid of iPBS markers and model-based clustering. It was concluded based on present findings that iPBS markers could reliably be used in genetic diversity and molecular analysis of oregano genotypes.
  • [ X ]
    Öğe
    Dissection of genetic diversity and population structure in oregano (Origanum acutidens L.) genotypes based on agro-morphological properties and start codon targeted (SCoT) markers
    (Springer, 2022) Karagoz, Halit; Hosseinpour, Arash; Karagoz, Fazilet Parlakova; Cakmakci, Ramazan; Haliloglu, Kamil
    Besides aromatic characteristics of the other oregano species, essential oil of Origanum acutidens has lethal effects on some cancer lines. This study was performed to determine genetic diversity in the 70 oregano (Odostomia acutidens) genotypes based on several agro-morphological traits and start codon targeted (SCoT) markers in the endemic spread zone of O. acutidens. The result of principal component analysis (PCA) revealed that the first two components accounted for 79.56% of the total agro-morphological variation. Cluster analysis based on phenotypic data grouped all investigated genotypes into three main clusters. GGE Biplot analysis indicated that genotype numbers as G35, G11, G24, G10, and G22 with the lowest interaction with different environments were more stable than the others ones. Taking into account all analyses, genotype numbers G36, G12, G10, and G33 were selected as ideal materials. In the molecular section, 10 SCoT primers generated 109 polymorphic fragments. The mean value of polymorphic information content (PIC) was 0.36. The mean number of effective alleles (Ne), Nei's genetic diversity (H), and Shannon's information index (I) were 1.63, 0.38, and 0.57, respectively. The unweighted pair-group method with arithmetic mean (UPGMA) algorithm grouped the 70 investigated genotypes into three main clusters. Mantel test showed no correlation between genetic distance and geographical distance in these individual (r = 0.01, p = 0.69). Moreover, population structure analysis showed that all genotypes formed three sub-populations. In general, population structure of the 70 investigated oregano genotypes collected from different origins of Turkey were successfully characterized with the aid of SCoT markers. Our results suggest that evaluation of genetic diversity and structure analysis in the natural populations could efficiently provide comprehensive information for conservation of endemic and endangered species that can be used in future breeding programs.
  • [ X ]
    Öğe
    Two or more bacterial mixtures increase tea plant growth, yield, nutrient intake and antioxidant enzyme activity
    (Taylor & Francis Inc, 2023) Erduran, Erdim; Cakmakc, Ramazan; Karagoz, Fazilet Parlakova; Karagoz, Halit
    This study was carried out in order to determine bacterial strains that can be used as biological fertilizer in tea cultivation. The present study assessed possible effects of mineral NPK fertilizer (TF: 80 kg ha(-1) compound 25: 5:10) and ACC deaminase-containing, N-2-fixing, and P-solubilizing 6 bacteria strains as single (T-1-T-6:Pseudomonas fluorescens RC483, Pseudomonas fluorescens RC77E, Pseudomonas putida RC19b, Bacillus licheniformis RC5B2, Bacillus subtilis RC5A2, Bacillus pumilus RC7A1), binary (T-7: RC483 + RC5A2, T-8: RC77E + RC7A1), triple (T-9: RC77E + RC5A2+ RC5B2, T-10: RC483+ RC19b + RC7A1) and quadruple (T-11: RC77E + RC5B2 + RC7A1+ RC5A2) inoculations, chemical fertilizer (80 kg ha(-1) compound 25: 5: 10) and control treatments on growth, yield, yield, nutrient uptake and enzyme activity of Pazar-20 tea clones manufactured for commercial purposes. Experimental groups determined in the present study were formed by considering the results of our preliminary study and bacteria that do not have antagonistic effects on each other. Results showed that dual, triple and quadruple inoculations stimulated overal second and third leaf area, leaf yield, macro- and micro-nutrient concenrations, chlorophyll and anthocyanin content, and antioxidant, oxidative, catalytic, hydrolytic and quality-related enzymes activity. Tea plant growth responses were dependent on the inoculant strain and formulations used, as well as on the parameters evaluated. In conclusion, bacterial seed inoculations especially with quadruple bacterial formulation determined to be most effective in tea growth can improve tea yield and productivity on sustainable basis. The ability to increase enzyme activity, which may be important in taste and aroma, leaf antioxidant, oxidative pentose phosphate pathway (OPPP) and tea processing technology by using different combinations prepared with bacterial isolates will be able to a significant contribution to tea technology studies.