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    Development of a novel aptasensor using jellyfish collagen as matrix and thrombin detection in blood samples obtained from patients with various neurodisease
    (Elsevier Science Sa, 2016) Derkus, Burak; Arslan, Yavuz Emre; Bayrac, Abdullah Tahir; Kantarcioglu, Ilkim; Emregul, Kaan C.; Emregul, Emel
    In the present study, we describe the isolation and detailed characterization of pepsin-soluble atelocollagen from Rhizostoma pulmo species jellyfish and application towards thrombin apta-sensing. Various analysis methods including infra-red spectroscopy, SDS-PAGE electrophoresis, and amino acid analysis have been applied for the characterization of jellyfish collagen and compared with both rat tail collagen and BSA. When comparing the two collagen types derived from jellyfish and rat tail, jellyfish collagen was observed to contain a relatively high amount of glutamic acid (61 residues/1000 residues) and alanine (63 residues/1000 residues) but low amounts of proline (113 residues/1000 residues). On the other hand, pepsin-soluble jellyfish collagen contained a small quantity of tyrosine indicating the purity of atelo-collagen. Electrochemical impedance spectroscopy is the main analyzing technique of the developed apta-sensor. The proposed apta-sensor has a detection limit of 6.25 nM thrombin. Clinical application were performed with analysis of the thrombin levels in blood and CSF samples obtained from patients with Multiple Sclerosis, Myastenia Gravis, Epilepsy, Parkinson, Polyneuropathy and healthy donors using both the apta-sensor and commercial ELISA kit. The results revealed the proposed system to be a promising candidate for clinical analysis of thrombin. (C) 2016 Elsevier B.V. All rights reserved.
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    Salvadora persica extract-laden jellyfish collagen hybrid constructs for periodontal tissue regeneration
    (Turkish Chemical Society, 2019) Arslan, Yavuz Emre; Kantarcioglu, Ilkim
    Considerable effort in the field of periodontal tissue engineering has been expended in the construction of advanced biomatrix for the treatment of periodontal diseases caused by poor oral hygiene, malnutrition, genetic factors, and systemic disorders. With these in mind, the ultimate goal of this investigation is to fabricate sophisticated scaffolds using jellyfish collagen (JC) and aqueous Salvadora persica (Miswak) extracts. Rhizostoma pulmo species JC was isolated and characterized in depth. Miswak was extracted using two different methods. The extraction yield was calculated to be 14.2 ± 0.9% and 17.1 ± 0.4% for Methods I and II, respectively. Gas chromatography-mass spectroscopy (GC-MS) results revealed the extract to be composed of 1,8-cineole (49.3%), benzyl nitrile (36.2%), benzyl isothiocyanate (5.9%), limonene (2.4%), eugenol (0.8%), and palmitic acid (0.3%). Total phenolic content and antioxidant capacities of the extracts were also determined by spectrophotometric means. Human periodontal ligament fibroblast cells were isolated and expanded. Cell viability on JC and miswak extract-laden JC scaffolds was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium Bromide (MTT) assay. Microarchitectures of the JC, 0.05 and 0.1% miswak extract-laden JC scaffolds and also cellular behaviors on these surfaces were evaluated by scanning electron microscopy (SEM) analysis. This study suggests that miswak extract-laden JC scaffolds would present new opportunities for periodontal tissue engineering. © 2019, Turkish Chemical Society. All rights reserved.