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    Ameliorative Effects of Ulva rigida (C. Agardh, 1823) on Cadmium-induced Nephrotoxicity in Wistar Albino Rats
    (Univ Agriculture, Fac Veterinary Science, 2022) Irkin, Latife Ceyda; Ozturk, Samil
    Many organs and systems, especially the kidney, are damaged by the effect of high-dose cadmium. These functional problems vary depending on the distribution of cadmium in the body, the way it is taken, the dose and the duration. Antioxidant activities of algae are due to the polyphenols, polysaccharides, pigments and vitamins they contain. Antioxidants are effective in protecting the body against damage caused by reactive oxygen species. This study was carried out to determine the therapeutic effects of Ulva rigida extract on kidney tissue fibrosis, inflammatory inflammation and apoptosis in cadmium-induced rats. In the study, 1 mg/kg CdCl2 was injected subcutaneously to the subjects four times a week for one month (G2). Concurrently, 50 mg/kg (G3) and 100 mg/kg (G4) algae extract were given to the subjects by gavage. In staining with hematoxylin-eosin, mononuclear cell infiltration in the kidney tissues, dilatation in the tubules, shrinkage of the glomeruli and degeneration of epithelium occurred with the induction of Cd. Immunohistochemically, it was determined that TGF-beta, TNF-alpha and NF-kB immunoreactivity was high due to tubular cells undergoing inflammation and apoptosis. Histopathological data show that U. rigida extract has a protective role against kidney damage in Cd-induced rats. Algae extract was found to play a protective role against cadmium-induced kidney damage. It was also found to have an important role as a therapeutic agent against oxidative stress.
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    ANTI-APOPTOTIC AND PROLIFERATIVE EFFECT OF MODERATE EXERCISE ON TESTICULAR TISSUE IN STREPTOZOTOCIN-INDUCED RATS
    (Parlar Scientific Publications (P S P), 2022) Ozturk, Samil; Ozdemir, Ilhan; Kamalak, Zeynep; Irkin, Latife Ceyda
    DM causes damage in many tissues and organs including the reproductive system. Severe damage in the male reproductive system is an important complication of DM. The aim of this study was to examine the effects of moderate exercise on testicular tissue in streptozotocin-diabetic (STZ) rats and to investigate the possible positive effects of exercise on the histological findings. Thirty adult rats were assigned to three groups: (a) control, (b) diabetes (40 mg/kg STZ) and (c) diabetes moderate exercise. The moderate exercise group started the exercise program 3 days before the induction of diabetes. After four weeks, the testes tissues of the rats were resected. Histochemical staining of the collected tissue specimens and morphometric tests were performed. Our study showed a reduction in the testis weight in the diabetes groups. The testis tissue of the control group was normal. Despite the atrophic changes, primary spermatocytes and spermatids were available in the testis tubules of the diabetic group. In the moderate exercise group, the architecture of the seminiferous tubules was close to normal in most of the specimens and the microscopic findings were not significantly different from those of the control group. Immunohistochemical results revealed that PCNA and TUNEL positive cells were significantly increased in the diabetic groups compared to the control group (p<0.001). The findings of our study supported that exercise had a significantly favorable effect on infertility, which is a complication of diabetes. Further molecular studies are required on this subject.
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    Immunohistochemical investigation of histopathological changes in the kidney tissue of mussel-fed rats
    (Cukurova Univ, Fac Medicine, 2020) Irkin, Latife Ceyda; Orturk, Samil
    Purpose: The aim of this study was to determine the histopathological changes that the mussels, which serve as important filters of the sea, cause in rat kidney tissue. Materials and Methods: In our study, 24 female (Wistar albino 6-10 week old rats) rats were used. First group (n: 6): fed with standard rat food, second group (n: 6): fed everyday with 4/5 mussels + 1/5 standard rat food; third group (n: 6): fed 4/5 mussels + 1/5 standard rat food every two days; Fourth group (n: 6): were formed with 4/5 mussels + 1/5 standard rat food to be given every three days. After the routine histopathological follow-up, all kidney tissue samples taken and control groups were analyzed with Hematoxylin-Eosin staining, and the nephrotoxic effect was immunohistochemically with TGF-beta and NF-kappa B and analyzed with a light microscope image analysis system. Results: Mussel-fed rats were found to cause inflammation in the kidney tissue, dilatation in the distal and proximal tubules, shrinkage in the glomeruli and degeneration in the tubular epithelium. In immunohistochemical staining, TGF-beta and NF-kappa B immunoreactivity increased due to cells leading to inflammation and apoptosis. The nephrotoxic effect was quite high especially in rats given daily mussels. Conclusion: The mussel provided in unsanitary conditions may cause damage in kidney tissue from the excretory system organs due to excess consumption.
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    Protective effect of Spirulina in the ovary of rats against Doxorubicin toxicity
    (Univ Zulia, Facultad Ciencias Veterinarias, 2024) Ozturk, Samil; Irkin, Latife Ceyda
    Doxorubicin (Dox) is an anti-cancer agent used of ovarian, breast, liver, lung cancers and solid tumors such as leukemia and lymphoma treatment. However, since it is an antineoplastic agent with a high toxic effect, it causes toxicity on many organs and tissues in the organism. Spirulina spp. (SP) contains phytopigments such as beta- carotene, tocopherols and xanthophylls and is a natural source of vitamin A as well as its anticarcinogenic effect. For these reasons, this study was planned to experimentally reveal the antioxidant and protective effects of SP on Dox-induced reproductive toxicity in female rats. In the study, histopathological evaluation was performed after Hematoxylin-Eosin staining in female rats with Dox toxicity. The distribution of GSK-3 beta for cell proliferation, HIF-1 alpha for oxidative stress and VEGF for vascularization were evaluated. TUNEL staining was performed to determine apoptosis. It was determined that SP prevented tissue damage on the ovarian tissue of rats with Dox toxicity, and this positive effect was achieved through factors such as oxidative stress (HIF-1 alpha), vascularization (VEGF) and inhibition of cell death (Apoptosis). It has been observed that SP treatment to be applied after chemotherapy is effective in protecting both the developing follicles and the primordial follicle pool.
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    Protective Effects of Curcumin and Resveratrol on Kidney Tissue on Cadmiuminduced Oxidative Stress in Rats
    (Galenos Publ House, 2024) Cakina, Suat; Irkin, Latife Ceyda; Ozturk, Samil
    Objective: Cadmium (Cd) is a well-known widespread environmental pollutant and is not rapidly excreted by the kidneys; instead, it accumulates and causes kidney damage. This study aimed to compare the effects of antioxidant curcumin and resveratrol on antioxidant defense in Cd-induced rat kidney tissue. Methods: In the study, 36 (200-250 gr) Wistar albino rats were divided into 6 (n=6) groups. Group 1: control; group 2: CdCl2; group 3: curcumin; group 4: CdCl2 + curcumin; group 5: resveratrol; group 6: CdCl2 + resveratrol. At the end of the experiment, malondialdehyde (MDA), total oxidant capacity (TOC), and total antioxidant capacity (TAC) activities were measured in kidney tissues. Results: In the CdCl2-treated group, oxidative stress index (OSI), TOC, and MDA levels increased compared with the control group, and TAC values decreased (p<0.05). In the case of resveratrol or curcumin administered with Cd, TAC levels increased, MDA levels, and OSI values decreased compared with the group administered only Cd (p<0.05). Conclusion: Both resveratrol and curcumin may have protective effects in the kidneys against CdCl2-induced oxidative damage.
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    The effect of heavy and alkaline metal accFmflation on the chlorophyll content of Fontinalis antipyretica Hedw. And Marchantia polymorpha L. And Ida Mofntain
    (Parlar Scientific Publications, 2019) Yayintas, Ozlem Tonguc; Irkin, Latife Ceyda; Yilmaz, Selehattin
    Fontinalis antipyretica Hedw. is specially a pollutant-sensitive indicator species in fresh water and Marchantia polymorpha L. is commonly found on moist clay or silty soils, especially on recently burned land throughout the northern hemisphere. F. antipyretica and M. polymorpha were examined to determine the quantity of metal accumulation. Samples were collected from Ayazma Stream (Ida Mountain) with similar exposure conditions in July 2017. Mosses are suitable species for the uptake of heavy metals because of their morphological and physiological properties. Because of this, studies on the use of mosses as an environmental monitoring have increased in last decades. In the present study each sample were irrigated for 72 hours with solutions 0, 02 M CuCl2, ZnCl2 and Pb(NO3)2. At the end of the experimental period, the chlorophyll concentration of F. antipyretica and M. polymorpha was decreased. There was significant reduction of the chlorophyll content for each sample due to the heavy metals accumulation. © by PSP
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    THE EFFECT OF HEAVY AND ALKALINE METAL ACCUMULATION ON THE CHLOROPHYLL CONTENT OF FONTINALIS ANTIPYRETICA HEDW. AND MARCHANTIA POLYMORPHA L. FROM IDA MOUNTAIN
    (Parlar Scientific Publications (P S P), 2019) Yayintas, Ozlem Tonguc; Irkin, Latife Ceyda; Yilmaz, Selehattin
    Fontinalis antipyretica Hedw. is specially a pollutant-sensitive indicator species in fresh water and Marchantia polymorpha L. is commonly found on moist clay or silty soils, especially on recently burned land throughout the northern hemisphere. F. antipyretica and M. polymorpha were examined to determine the quantity of metal accumulation. Samples were collected from Ayazma Stream (Ida Mountain) with similar exposure conditions in July 2017. Mosses are suitable species for the uptake of heavy metals because of their morphological and physiological properties. Because of this, studies on the use of mosses as an environmental monitoring have increased in last decades. In the present study each sample were irrigated for 72 hours with solutions 0, 02 M CuCl2, ZnCl2 and Pb(NO3)2. At the end of the experimental period, the chlorophyll concentration of F. antipyretica and M. polymorpha was decreased. There was significant reduction of the chlorophyll content for each sample due to the heavy metals accumulation.
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    The effect of Ulva rigida (C. Agardh, 1823) against cadmium-induced apoptosis and oxidative stress
    (Sciendo, 2022) Irkin, Latife Ceyda
    Cadmium (Cd) is known as a pollutant source in recent years with the increase in industrialization. Algae have secondary metabolites with high biological activity, used for pharmaceutical agents. The liver and kidney are the primary organs involved in the elimination of systemic cadmium and the main targets of cadmium toxicity. In the previous research, it was determined the ameliorative effects of the extract obtained from Ulva rigida in the liver tissue of rats induced by cadmium. 35 female Wistar rats between 225-240 g were used. The subjects were injected subcutaneously with 1 mg/kg cadmium chloride (CdCl2) four times a week for four weeks. The study was carried out by groups as control (G1), Cadmium group (1 mg/kg CdCl2-G2), Algae group (100 mg/kg-G3), Cd+algae group (1 mg/kg CdCl2+50 mg/kg algae extract-G4) and Cd+algae group (1 mg/kg CdCl2+100 mg/kg algae extract -G5). The subjects were sacrificed by cervical dislocation. Liver tissue and cardiac blood were collected. It was determined that oxidative stress with iNOS, inflammation and apoptosis with TNF-alpha increased with cadmium induction, while there was a statistically significant decrease in the groups that were given algae extract. In addition, biochemical changes in SOD, CAT and MDA values were found to be significant (p<0.05). As a result, it was determined that algae extract could play a protective role with its antioxidant and antiapoptotic properties in experimentally induced cadmium toxicity in rats.