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Öğe Acute exposure to boron in Nile tilapia (Oreochromis niloticus): Median-lethal concentration (LC50), blood parameters, DNA fragmentation of blood and sperm cells(Pergamon-Elsevier Science Ltd, 2018) Acar, Umit; Inanan, Burak Evren; Zemheri, Fahriye; Kesbic, Osman Sabri; Yilmaz, SevdanRecently, Boron (B) contamination of aquatic ecosystem has received considerable critical attention due to its toxic effects at high concentrations on plants as well as animals. Previous studies on toxic effects of B have not dealt with DNA damages in blood and sperm cells of Nile tilapia (Oreochromis niloticus), together with blood parameters. This study consisted of two successive experiments purposes to determine these findings, including the median-lethal concentration (LC50) of B for Nile tilapia. In the first one, at 96 h, LC50 of B for Nile tilapia was 141.42 mg L-1 B. In the second one, Nile tilapia were treated with five elevated B concentrations (1, 5, 25, 50 and 100 mg L-1 B) and hematological, serum biochemical parameters, and DNA damages of Nile tilapia in these treatments were determined comparing with the control after 14 days of exposure. Hematological parameters (red blood cell count, hematocrit, and hemoglobin) were similar to each other in all groups while serum biochemical parameters (glucose, total protein, cholesterol, alkaline phosphatase, glutamic oxaloacetic transaminase, and glutamic pyruvic transaminase) in B treatments, especially in 50 and 100 mg L-1 B groups, were found significantly higher (P < 0.05). DNA fragmentation was detected in all blood cells in >= 25 mg L-1 B treatments, while all sperm cells in 100 mg L-1 B treatments. These results demonstrated the harmful effects of B at high concentration (50 and 100 mg L-1 B) on the DNA integrity of blood and sperm cells, and also the serum biochemical parameters. (C) 2018 Elsevier Ltd. All rights reserved.Öğe Comparative study on haematological and biochemical parameters of two wild sparid fish species(Cahiers De Biologie Marine, 2019) Acar, Umit; Saoca, Concetta; Kesbic, Osman Sabri; Yilmaz, Sevdan; Yigit, Murat; Inanan, Burak Evren; Fazio, FrancescoDifferent feeding habits may affect the blood parameters in fish species of the same family, even if they live in the same marine habitat. The aim of this study was to compare some haematological and biochemical parameters of two marine fish species of the same family, the saupe Sarpa salpa (Linnaeus, 1758) and the common two-banded seabream Diplodus vulgaris (Geoffroy Saint-Hilaire, 1817), and to establish the similarities and differences between these species. For this purpose red blood cells (RBC), haemoglobin concentration (Hb), haematocrit (Hct), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), mean corpuscular haemoglobin concentration (MCHC), glucose (GLU), triglycerides (TRIG), cholesterol (CHOL), serum total protein (TP), albumin (ALB), globulins (GLO), aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP) and lactate dehydrogenase (LDH) were determined for 30 fish from each species. Statistical analysis showed significant differences in some haematological and biochemical (RBC, Hb, Hct, TP, ALB, GLO, AST, ALT, ALP and LDH) parameters between the two species. Lower values of RBC, Hb and Hct were found in S. salpa in comparison with D. vulgaris. These differences could be attributed to the feeding behavior, life style and habitat adaptation of the different fish species of the same family and contribute to knowledge of reference values of blood parameters in Sarpa salpa and Diplodus vulgaris. No significant differences were found in MCV, MCH, MCHC and GLU, TRIG, CHOL.Öğe Ecotoxicological effects of polystyrene nanoplastics on common carp: Insights into blood parameters, DNA damage, and gene expression(Wiley, 2024) Acar, Umit; Inanan, Burak Evren; Zemheri-Navruz, FahriyePlastics are ubiquitous in modern society due to their cost-effectiveness, lightweight nature, and versatility. However, their extensive use and inadequate recycling have led to a significant environmental challenge, with plastic waste accumulating rapidly and causing ecological and health problems, especially in aquatic environments. Nanoplastics, particles ranging from 1 to 100 nm, have emerged as a particularly concerning subset due to their ability to easily penetrate biological barriers and accumulate in tissues. In this study, we investigated the toxicity of carboxylate-modified polystyrene nanoplastics (PS-NPs) on common carp (Cyprinus carpio), a species often used in ecotoxicology research due to its ability to accumulate pollutants. The PS-NPs were characterized, and their effects on DNA damage gene expression related to oxidative stress and immunity were examined. PS-NPs with a diameter of 20-30 nm were found to possess a spherical shape and negatively charged surfaces. Exposure to PS-NPs led to significant DNA damage in the blood and brain cells of common carp, with higher concentrations resulting in more severe damage. Additionally, PS-NP exposure influenced the expression of genes related to antioxidative defense and stress response in the liver. Specifically, genes encoding superoxide dismutase (SOD), catalase (CAT), and heat shock protein 70 (Hsp70) showed upregulation, while glutathione peroxidase (GPx) and glutathione S-transferase (GST) exhibited downregulation at higher PS-NP concentrations. Furthermore, the immune-related genes interleukin-1 ss (IL-1 ss), interleukin-8 (IL-8), and tumor necrosis factor-alpha (TNF-alpha) displayed dose-dependent downregulation in the liver tissue. These findings suggest that exposure to PS-NPs induces oxidative stress, disrupts immune responses, and causes DNA damage in common carp. The results highlight the need for further research on the environmental impacts of PS-NPs and underscore the importance of proper waste management and recycling practices to mitigate plastic pollution. Plastics are widely used because of their affordability, versatility, and lightness. However, their inadequate recycling has led to environmental challenges, notably in aquatic ecosystems. Nanoplastics pose significant concerns due to their ability to accumulate in tissues. In this study on common carp, exposure to PS-NPs resulted in DNA damage, altered gene expression related to oxidative stress and immunity, and disrupted immune responses. These findings emphasize the urgent need for better waste management and recycling practices to combat plastic pollution.Öğe Effects of dietary Bergamot (Citrus bergamia) peel oil on growth, haematology and immune response of European sea bass (Dicentrarchus labrax) juveniles(Wiley, 2019) Acar, Umit; Kesbic, Osman Sabri; Inanan, Burak Evren; Yilmaz, SevdanThe aim of the present study was to evaluate the effects of essential oil extracted from bergamot (Citrus bergamia) peel on growth performance and health status of European sea bass juveniles (5.10 + 0.05 g). Test diets supplemented with 0.0%, 0.5%, 1.0% and 2.0% bergamot peel oil (BPO0, BPO0.5, BPO1 and BPO2) were fed to experimental fish for 60 days. At the end of the experiment, the best growth performance was obtained in the BPO0.5 and BPO1 groups (p < .05). No significances were found for haematological parameters including red blood cell count, haematocrit and haemoglobin concentration at the end of the feeding experiment. There were significant differences between the total protein, cholesterol, alkaline phosphatase and glutamic oxaloacetic transaminase (p < .05) between the BPO0.5 and BPO1 supplemented groups and the control group with no BPO addition. The addition of BPO showed significant differences in immune parameters such as lysozyme activity, total myeloperoxidase content, superoxide dismutase and glutathione peroxidase activity between BPO0 and BPO supplemented groups (p < .05). It might be concluded that dietary addition of 0.5%-1% BPO could increase the growth performance and feed utilization with improved health status in sea bass juveniles.
