Yazar "Ilhan, Dogan" seçeneğine göre listele

Listeleniyor 1 - 3 / 3
  • [ X ]
    Öğe
    EVALUATION OF GENETIC EFFECTS OF SOME GENOTOXIC COMPOUNDS IN Tradescantia pallida H. WITH MICRONUCLEUS AND STAMEN-HAIR MUTATION TESTS
    (Parlar Scientific Publications (P S P), 2009) Ilhan, Dogan; Akı, Cüneyt
    The aim of this study is to evaluate Sunset Yellow (E I 10) and Brilliant Black (E 15 1) food-coloring substances which are putative to genotoxic activity, and commonly used in food industry with Tradescantia pallida micronucleus (Trad-MCN) test and Tradescantia pallida stamen-hair mutation (Trad-SHM) test. The research project included 4 concentrations (10, 200, 1000, 2000 ppm) of both food colors applied to plants by spraying and adding to the irrigation water. All experiments were carried out under controlled conditions in the laboratory. According to the results, it was observed that mutation levels increased, depending on the dosage, in all plant groups. In addition, both MCN frequency and pink mutation events significantly increased (p<0.05) with increasing dosage. It was also detected that both food-coloring agents had genotoxic potential on plants with different sensitivities. It was revealed that the potential effect of Sunset Yellow (E110) can be analyzed better with Trad-SHM and the effect of Brilliant Black (E151) with Trad-MCN tests. Results confirm that Tradescantia pallida plant is useful for biomonitoring studies of mutagenesis.
  • [ X ]
    Öğe
    MUTAGENICITY OF SUNSET YELLOW AND BRILLIANT BLACK IN Vicia faba L. AND Allium cepa L.
    (Parlar Scientific Publications (P S P), 2010) Ilhan, Dogan; Akı, Cüneyt
    The objective of the present study was to apply highly sensitive botanical tests of mutagenicity (Vicia faba L. root tip mitosis test, micronucleus test, and A Ilium cepa L. root tip mitosis test) to assess in vitro the biological responses induced by food-coloring substances. The mutagenesis was evaluated using two different food-coloring agents which are Sunset Yellow (E 110) and Brilliant Black (E 151). For mutagenesis, four different concentrations (10, 200, 1000, 2000 ppm) of both food colorants were applied to plants by spraying and adding to the irrigation water. All experiments were carried out under controlled conditions in the laboratory. The results consistently showed that mutation levels increased, depending on the dosage, in all plant groups. In addition, while micronucleus frequency (MCN) significantly increased (p<0.05) and mitotic indexes significantly decreased (p<0.05) with the increasing dosages in Vicia faba L. and A Ilium cepa L. It was also detected that both food-coloring agents had genotoxic potential on plants with different sensitivities and Vicia faba L. or A Ilium cepa L. plants are appropriate for mutagenicity studies.
  • [ X ]
    Öğe
    THE EFFECTS OF GLYPHOSATE ISOPROPYLAMINE ON MITOTIC ACTIVITY, SUPEROXIDE DISMUTASE AND PEROXIDASE ACTIVITIES OF Allium CEPA L. ROOT TIP CELLS
    (Parlar Scientific Publications (P S P), 2010) Acar, Okan; Demirbas, Sefer; Ilhan, Dogan; Ozdinc, Nagme
    Glyphosate is a broad-spectrum herbicide that can widely be used in agricultural and non-agricultural fields. Glyphosate isopropylamine affects the inhibition of the biosynthesis of aromatic amino acids, and also that of some enzymes involved in the shikimic acid pathway. The aim of this research was to study the effects of different concentrations of glyphosate isopropylamine (0.5, 1, 2 and 5%) on the mitotic activity in Allium cepa L. root tip cells and the superoxide dismutase (SOD) and peroxidase (POX) activities in Allium cepa L. roots. Glyphosate isopropylamine concentrations of 0.5, 1 and 2% did not lead to a significant change in the mitotic activity compared to the control group. However, for the concentration of 5%, a steep decline was observed in 48-h treatment of the substance, in comparison to that of 24 h. Total SOD activity decreased in the 24-h treatment group of glyphosate isopropylamine compared to the control group, whereas with the concentrations of 2 and 5% no SOD activity was observed. However, the POX activity increased only with 1% concentration, with regard to the control group, whereas it did not change with the other concentrations.