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    Antibiotic Resistance Profiles and Biochemical Characterization of Bacteria Isolated from Gökçeada Salt Lake Lagoon (Çanakkale) Sediment Samples
    (Sakarya University, 2025) Karakaş, İlke; Hacioglu-Dogru, Nurcihan
    Gökçeada Salt Lake Lagoon in Çanakkale, Türkiye, is a hypersaline environment that supports halophilic bacterial communities. This study examined the antibiotic susceptibility of 60 bacterial isolates obtained from four seasonal sediment samples. Morphological and biochemical characterization of the isolates included Gram staining, indole production, nitrate formation tests, and elemental analysis using ICP-OES. Antibiotic resistance was assessed via the disk diffusion method using seven antibiotics: Novobiocin (5 ?g), Erythromycin (15 ?g), Streptomycin (10 ?g), Bacitracin (10 ?g), Penicillin G (10 ?g), Ampicillin (10 ?g), and Tetracycline (30 ?g). ICP-OES results showed elevated S, Ca, Mg, Na, and Fe concentrations, particularly in April 2024. The Multiple Antibiotic Resistance (MAR) index exceeded 0.2 in all isolates, with the highest value (0.7142) also detected in April 2024. The Shapiro-Wilk test indicated non-normal distribution of MAR values; thus, the Kruskal-Wallis test was used for statistical analysis. Although no significant difference was found between sampling periods (p > 0.05), seasonal increases in resistance suggest gradual adaptation. The consistently high MAR indices point to ongoing anthropogenic pressure, likely due to tourism, agriculture, and livestock activities in the region. This persistent exposure may drive resistance development in microbial populations. The findings underscore the importance of monitoring antibiotic resistance in halophilic bacteria, as such resistance may pose public health risks through environmental pathways. © 2025, Sakarya University. All rights reserved.
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    Monitoring of micronuclei and nuclear abnormalities In Pelophylax ridibundus erythrocytes from the Biga Stream (Canakkale, Turkey)
    (Parlar Scientific Publications, 2018) Corduk, Nursen; Hacioglu-Dogru, Nurcihan; Gul, Cigdem; Tosunoğlu, Murat
    In this study, the frequency of micronuclei and nuclear abnormalities caused by environmental pollutants were determined in Pelophylax ridibundus from the Biga Stream (Canakkale, Turkey). Healthy and mature animals were collected by hand capture method from along the four stations (estuary zone, city center, buffer zone and industrial zone) of Biga Stream. Blood samples of the live specimens were obtained in the laboratory within one day of their capture. Two peripheral blood smears for each sampled specimen were prepared on clean slides, fixed with methanol for 15 min and dyed with Giemsa stain. Micronuclei and other nuclear abnormalities such as kidney shaped nuclei, lobed nuclei, and notched nuclei and blebbed nuclei were determined and scored in the slides. Also, heavy metal analysis was carried out to determine the pollution level of the waters in the stations. Our result have shown that the frequency of nuclear abnormalities were highest in Station 2 (8.64±0.51). However, the station 2 has higher rates of values of Al, Cu, Zn and Fe than the values obtained from the other stations. The results have showed that the amount of heavy metals contained in the stations was found to be close parallels with the percentage of the total nuclear abnormalities and each kind of abnormality in P. ridibundus erythrocytes. The results have indicated that the levels of genotoxic pollutants in different regions of the Biga stream differ and lead to DNA damage on the organisms. © by PSP.
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    Öğe
    MONITORING OF MICRONUCLEI AND NUCLEAR ABNORMALITIES IN PELOPHYLAX RIDIBUNDUS ERYTHROCYTES FROM THE BIGA STREAM (CANAKKALE, TURKEY)
    (Parlar Scientific Publications (P S P), 2018) Corduk, Nursen; Hacioglu-Dogru, Nurcihan; Gul, Cigdem; Tosunoğlu, Murat
    In this study, the frequency of micronuclei and nuclear abnormalities caused by environmental pollutants were determined in Pelophylax ridibundus from the Biga Stream (Canakkale, Turkey). Healthy and mature animals were collected by hand capture method from along the four stations (estuary zone, city center, buffer zone and industrial zone) of Biga Stream. Blood samples of the live specimens were obtained in the laboratory within one day of their capture. Two peripheral blood smears for each sampled specimen were prepared on clean slides, fixed with methanol for 15 min and dyed with Giemsa stain. Micronuclei and other nuclear abnormalities such as kidney shaped nuclei, lobed nuclei, and notched nuclei and blebbed nuclei were determined and scored in the slides. Also, heavy metal analysis was carried out to determine the pollution level of the waters in the stations. Our result have shown that the frequency of nuclear abnormalities were highest in Station 2 (8.64 +/- 0.51). However, the station 2 has higher rates of values of Al, Cu, Zn and Fe than the values obtained from the other stations. The results have showed that the amount of heavy metals contained in the stations was found to be close parallels with the percentage of the total nuclear abnormalities and each kind of abnormality in P. ridibundus erythrocytes. The results have indicated that the levels of genotoxic pollutants in different regions of the Biga stream differ and lead to DNA damage on the organisms.