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Öğe A STUDY ON MICROFLORA IN ORAL AND CLOACAL OF FRESHWATER TURTLES (Emys orbicularis Linnaeus, 1758 and Mauremys rivulata Valenciennes, 1833) FROM KAVAK DELTA (CANAKKALE)(Parlar Scientific Publications (P S P), 2012) Hacioglu, Nurcihan; Dulger, Basaran; Caprazli, Tugce; Tosunoğlu, MuratDeltas are reservoirs for important plant and animal species and have a vital role in maintaining sustainability of ecological balance. In addition, because of anthropogenic pressure they are recognized as natural habitat of pathogenic microorganisms and infectious diseases caused by these bacteria are dangerous to aquatic animal's health and could potentially affect several species as well those are endangered. This study aims to determine the micro-flora of freshwater turtles (Emys orbicularis Linnaeus, 1758 and Mauremys rivulata Valenciennes, 1833) and sensibilities of these turtles against isolated pathogenic bacteria. Furthermore the investigation was conducted to characterize diseases causative agents. Their risks to animal and human health are difficult to carry out, especially for some turtle species. The results showed that the isolates were identified as Vibrio spp. and Proteus spp. So, these bacteria are the main pathogens isolated from E. orbicularis and M. rivulata. These data appoint the importance of epidemiological surveillance and microbiological monitoring and reinforce the need to implement environment protection programs especially for the threatened turtle species (E. orbicularis).Öğe A study on microflora in oral and cloacal of freshwater turtles (Emys orbicularis Linnaeus, 1758 and Mauremys rivulata Valenciennes, 1833) from kavak delta (Canakkale)(2012) Hacioglu, Nurcihan; Dulger, Başaran; Caprazli, Tu?çe; Tosunoğlu, MuratDeltas are reservoirs for important plant and animal species and have a vital role in maintaining sustainability of ecological balance. In addition, because of anthropogenic pressure they are recognized as natural habitat of pathogenic microorganisms and infectious diseases caused by these bacteria are dangerous to aquatic animal's health and could potentially affect several species as well those are endangered. This study aims to determine the microflora of freshwater turtles (Emys orbicularis Linnaeus, 1758 and Mauremys rivulata Valenciennes, 1833) and sensibilities of these turtles against isolated pathogenic bacteria. Furthermore the investigation was conducted to characterize diseases causative agents. Their risks to animal and human health are difficult to carry out, especially for some turtle species. The results showed that the isolates were identified as Vibrio spp. and Proteus spp. So, these bacteria are the main pathogens isolated from E. orbicularis and M. rivulata. These data appoint the importance of epidemiological surveillance and microbiological monitoring and reinforce the need to implement environment protection programs especially for the threatened turtle species (E. orbicularis).Öğe Antibacterial activity of endemic Lamium tenuiflorum(Asian Journal Of Chemistry, 2008) Dulger, Basaran; Hacioglu, NurcihanThe organic solvents and aqueous extracts obtained from the leaves, rootstock and the combined formulation of endemic Lamium tenuiflorum Fisch. & Mey. (Lamiaceae) were tested for their antimicrobial activity against Escherichia coli ATCC 11230, Staphylococcus aureus ATCC 6538P, Klebsiella pneumoniae UC57, Pseudomonas aeruginosa ATCC 27853, Proteus vulgaris ATCC 8427, Bacillus cereus ATCC 7064, Mycobacterium smegmatis CCM 2067, Listeria monocytogenes ATCC 15313, Micrococcus luteus CCM 169 by the well-in-agar method. All the organic solvent extracts exhibited a strong antibacterial effect against the bacterial cultures except for the aqueous extracts, which had no effect.Öğe Antifungal activity of endemic Satureja icarica(Asian Journal Of Chemistry, 2008) Dulger, Basaran; Hacioglu, NurcihanThe methanol extract obtained from Satureja icarica P. H. Davis (Lamiaceae) have been investigated for their antifungal activity. Antifungal activity was determined with Candia albicans, Candida tropicalis, Candida guilliermondii, Cryptococcus neoformans, Cryptococcus laurentii, Aspergillus flavus, Aspergillus niger Alternaria alternate, Geotrichum candidum, Fusarium oxysporum, Penicillium frequentans, Penicillium canescens and Botrytis cineriae by the well-in-agar method and the visual broth macrodilution method. The diameter of inhibition zones varied from 11.6 to 18.8 mm. The MIC values ranged from 6.25 to 25 mu g/mL. The extracts exhibited a strong antifungal effect against the tested fungi. Candida albicans is more susceptible to the extract. The present findings provide a support to the use of this plant in traditional medicine for fungal infections especially against candidiasis.Öğe Antifungal Activity of Seeds of Hyoscyamus niger L. (Henbane) Against Some Clinically Relevant Fungal Pathogens(Asian Journal Of Chemistry, 2010) Dulger, Basaran; Hacioglu, Nurcihan; Goncu, Beyza S.; Gucin, FahrettinThe methanolic extracts obtained from the seeds of Hyoscyamus niger L. (Solanaceae) used as traditional medicine in Turkey were investigated for their ability to inhibit clinically relevant fungal pathogens as six Candida species (C. albicans ATCC 10231, C. tropicalis ATCC 13808, C. guilliermondii ATCC 6260, C. krusei ATCC 20298, C. glabrata ATCC 2001 neoformans ATCC 90112 and C. laurentii ATCC 34142) by microbroth dilution method. The plant displayed activity against all fungal cultures tested. The extracts possessed a strong antifungal potency Greater activity was observed against both Cryptococcus species, with MIC values of 15 mu g/mL.Öğe Antifungal activity of seeds of hyoseyamus niger L. (Henbane) against some clinically relevant fungal pathogens(Chemical Publishing Co., 2010) Dulger, Basaran; Hacioglu, Nurcihan; Goncu, Beyza S.; Gucin, FahrettinThe methanolic extracts obtained from the seeds of Hyoscyamus niger L. (Solanaceae) used as traditional medicine in Turkey were investigated for their ability to inhibit clinically relevant fungal pathogens as six Candida species (C. albicans ATCC 10231, C. tropicalis ATCC 13808, C. guilliermondii ATCC 6260, C. krusei ATCC 20298, C. glabrata ATCC 2001 and C. parapsilosis ATCC 22019) and two Cryptococcus species (C. neoformans ATCC 90112 and C. laurentii ATCC 34142) by microbroth dilution method. The plant displayed activity against all fungal cultures tested. The extracts possessed a strong antifungal potency. Greater activity was observed against both Cryptococcus species, with MIC values of 15 ?g/mL.Öğe Antimicrobial Activity of Bacopa caroliniana(Asian Journal Of Chemistry, 2009) Dulger, Basaran; Hacioglu, NurcihanEthanolic extract obtained from Bacopa caroliniana (Walt.) B.L.Robins (Scrophulariaceae) was investigated for its antimicrobial activity against Escherichia coli ATCC H 230, Staphylococcus aureus ATCC 6538P, Klebsiella pneumoniae UC57, Pseudomonas aeruginosa ATCC 27853, Proteus vulgaris ATCC 8427, Bacillus cereus ATCC 7064, Mycobacterium smegmatis CCM 2067, Listeria monocytogenes ATCC 15313, Micrococcus luteus CCM 169, Candida albicans ATCC 1023 1, Rhodotorula rubra DSM 70403, Kluyveromyces fragilis ATCC 8608, Debaryomyces hansenii DSM 70238 and Hanseniaspora guilliermondii DSM 3432 by disc-diffusion method. The extract has strong antimicrobial effects against the tested microorganisms especially the bacterium Staphylococcus aureus and the yeast culture Candida albicans. The findings support the use of this plant in traditional medicine.Öğe Antimicrobial Activity of Cotinus coggyria from Turkey(Asian Journal Of Chemistry, 2009) Dulger, Basaran; Hacioglu, Nurcihan; Bilen, S.Ethanolic extract obtained from Cotinus coggyria Scop. was investigated for its antimicrobial activity against Bacillus cereus, Bacillus subtilis, Staphylococcus aureus, Micrococcus luteus, Escherichia coli, Enterobacter aerogenes, Proteus vulgaris, Pseudomonas aeuginosa, Pseudomonas putida, Salmonella typhimurium, Salmonella typhi, Hanseniaspora guilliermondii, Rhodotorula rubra, Kluyveromyces fragilis, Kluyveromyces marxianus, Debaryomyces hansenii, Candida utilis and Candida albicans by disc diffusion method. The extracts had strong antimicrobial activity against bacteria, but weak activity was observed against the yeast cultures used in this study.Öğe Antimicrobial activity of endemic Hypericum havvae from Turkey(Asian Journal Of Chemistry, 2008) Dulger, Basaran; Hacioglu, Nurcihan; Dulger, Gorkemn-Hexane, ethyl acetate, ethanol and aqueous extracts of Hypericum havvae A.Guner (Hyperiaceae) were tested for their antimicrobial activity against Escherichia coli ATCC 11230, Enterobacter aerogenes ATCC 13048, Alcaligenes faecalis CCM 3763, Salmonella typhimurium CCM 5445, Citrobacter freundii ATCC 8090, Staphylococcus aureus 6538P, Bacillus cereus ATCC 7064, Bacillus subtilis ATCC 6633, Bacillus brevis ATCC 9999, Pseudomonas aeruginosa ATCC 27583, Proteus vulgaris ATCC 8427, Micrococcus luteus CCM 169, Micrococcus flavus ATCC 14452, Candida albicans ATCC 10231, Rhodotorula rubra DSM 70403 and Kluyveromyces fragilis ATCC 8608. While extracts of the plant have shown strong antimicrobial activity against the tested bacteria, they have weak activity against the tested yeast cultures.Öğe Antimicrobial Activity of Some Brown Algae from Turkey(Asian Journal Of Chemistry, 2009) Dulger, Basaran; Hacioglu, Nurcihan; Erdugan, Huseyin; Aysel, VeyselIn vitro antimicrobial screening of methanolic extracts obtained from 6 brown algae (Calpomenia peregrine (Sauvageau) Hamel, Halopteris scoparia (Linnaeus) Sauvageau, Padina pavonica (Linnaeus) Thivy, Cystoseria compressa (Esper) Gerloff et. Nizamuddin, Cladostephus spongiosus (Hudson) C.Agardh and Scytosiphon simplicissimus (Clemente) Cremades) (Heterokontophyta) collected from coast of Canakkale, Turkey, against Escherichia coli ATCC 11230, Staphylococcus aureus ATCC 6538P, Klebsiella pneumoniae UC57, Pseudomonas aeruginosa ATCC 27853, Proteus vulgaris ATCC 8427, Bacillus subtilis ATCC 6633, Mycobacterium smegmatis CCM 2067, Listeria monocytogenes ATCC 15313, Microccoccus luteus CCM 169, Candida albicans ATCC 1023 1, Rhodotorula rubra DSM 70403, Kluyveromyces fragilis ATCC 8608 and Cryptococcus neoformans ATCC 90112 was conducted in this study by disc diffusion method. Cystoseria compressa was the most active exhibiting a broad spectrum antimicrobial activity against each of the microorganims tested. Contrary to this, the lowest effect was observed for Calpomenia peregrine. The other algae species have moderate effects. The extracts showing good antimicrobial activity are undergoing further analysis to identify the active constituents.Öğe Antimicrobial activity of some endemic Verbascum and Scrophularia species from Turkey(Asian Journal Of Chemistry, 2008) Dulger, Basaran; Hacioglu, NurcihanThe methanol extracts obtained from three endemic Verbascum L. species (V. cariense Hub.-Mor., V. adenophorum Boiss. and V. inulifolium Hub.-Mor.) and two endemic Scrophularia L. species (S. depauperata Boiss. and S. mersinensis Lall) have been investigated for their antimicrobial activity. Antimicrobial activity was determined with Escherichia coli ATCC 11230, Staphylococcus aureus ATCC 6538-P, Klebsiella pneumoniae UC57, Pseudomonas aeruginosa ATCC 27583, Proteus vulgaris ATCC 8427, Bacillus cereus ATCC 7064, Mycobacterium smegmatis CCM 2067, Listeria monocytogenes ATCC 15313, Micrococcus luteus CCM 169, Candida albicans ATCC 10231, Rhodotorula rubra DSM 70403, Kluyveromyces fragilis ATCC 8608, Debaryomyces hansenii DSM 70238 and Hanseniaspora guilliermondii DSM 3432 by the disk diffusion method. The extracts of all plant species had strong antimicrobial activity against the Gram-positive bacteria and yeasts, but no activity was observed against the Gram-negative bacteria used in this study.Öğe Antimicrobial activity of three Macrolepiota species from Turkey(Asian Journal Of Chemistry, 2008) Dulger, Basaran; Hacioglu, Nurcihan; Aydin, Gurhan; Uzun, YusufThree Macrolepiota species (M. heimii, M. rachodes and M. puellaris) were tested for their antimicrobial activity by using the disc diffusion method. The ethanol extract from the fruit bodies of mushrooms were assayed against 16 microorganisms. In comparison with the test antibiotics amikacin, chloramphenicol and nystatin, the ethanol extract obtained from M. heimii presented significant activity against Micrococcus species and Citrobacter freundii. The extracts have weak anti-yeast activity against the yeast cultures.Öğe Antimicrobial potential of Xylaria polymorpha (Pers.) Grev.(Academic Journals, 2011) Hacioglu, Nurcihan; Akata, Ilgaz; Dulger, BasaranThe ethanolic extracts obtained from the macrofungus Xylaria polymorpha (Pers.) Grev. (Ascomycetes) have been investigated for their antimicrobial activity. Growth inhibition using agar disc diffusion assays was determined against Escherichia coli ATCC 11230, S. aureus ATCC 6538P, Pseudomonas aeruginosa ATCC 27853, Proteus vulgaris ATCC 8427, Bacillus cereus ATCC 7064, Micrococcus luteus CCM 169, Candida albicans ATCC 10231, Rhodotorula rubra DSM 70403, Geotrichum capitatum ATCC 28576, Debaryomyces hansenii DSM 70238, Kluyveromyces fragilis ATCC 8608 and Cryptococcus neoformans ATCC 90112. As a result of study, we have found that X. polymorpha revealed antimicrobial activity against all tested microorganisms. Especially, G. capitatum is more susceptible to the extracts.Öğe Antiyeast Activity of Ethanolic Extract of Bjekandera adusta(Asian Journal Of Chemistry, 2010) Dulger, Basaran; Hacioglu, Nurcihan; Akata, IlgazEthanolic extract of Bjekandera adusta (Wind.) P. Karst. was investigated for its antiyeast activity against Hanseniaspora guilliermondii, Rhodotorula rubra, Kluyveromyces fragilis, Kluyveromyces marxianus, Debaryomyces hansenii,Candida albicans. Candida glabrata, Candida utilis and Cryptococcus neoformans by disc diffusion method. The extracts. had strong effects against Candida albicans and Cryptococcus neoformans, but weak activity was seen against the other yeast cultures used in present study.Öğe Determination of antimicrobial and heavy metal resistance profiles of some bacteria isolated from aquatic amphibian and reptile species(Springer, 2014) Hacioglu, Nurcihan; Tosunoğlu, MuratThe aim of the present study was to determine the level of antibiotic resistance patterns and distribution of heavy metal resistance of bacterial isolates from aquatic animals (Lissotriton vulgaris, Pelophylax ridibundus, Emys orbicularis, Mauremys rivulata, and Natrix natrix) in Turkey (Kavak Delta). A total of 153 bacteria have been successfully isolated from cloaca and oral samples of the aquatic amphibians and reptilians which were found, namely, Aeromonas sp. (n = 29), Plesiomonas sp. (n = 7), Vibrio sp. (n = 12), Citrobacter sp. (n = 12), Enterobacter sp. (n = 11), Escherichia sp. (n = 22), Klebsiella sp. (n = 22), Edwardsiella sp. (n = 6), Hafnia sp. (n = 1), Proteus sp. (n = 19), Providencia sp. (n = 8), and Pseudomonas sp. (n = 4). In terms of antibiotic and heavy metal susceptibility testing, each isolate was tested against 12 antibiotics and 4 metals. There was a high incidence of resistance to cefoxitin (46.40 %), ampicillin (44.44 %), erythromycin (35.29 %), and a low incidence of resistance to gentamicin (6.53 %), kanamycin (8.49 %), chloramphenicol (9.15 %), and cefotaxime (10.45 %). The multiple antibiotic resistance index of each bacterial species indicated that bacteria from raised amphibians and reptiles have been exposed to tested antibiotics, with results ranging from 0 to 0.58. Most isolates showed tolerance to different concentrations of heavy metals, and minimal inhibition concentrations ranged from100 to > 3,200 mu g/mL. According to these results, a significant occurrence of bacteria in the internal organs of reptiles and amphibians, with a high incidence of resistance against antibiotics and heavy metals, may risk aquatic animals and the public health. These data appoint the importance of epidemiological surveillance and microbiological monitoring and reinforce the need to implement environment protection programs for amphibian and reptile species.Öğe Evaluation of Antimicrobial Activity of Some Mosses From Turkey(Asian Journal Of Chemistry, 2009) Dulger, Basaran; Hacioglu, Nurcihan; Uyar, GurayEthanolic extracts obtained from 5 Byrophyte species (Platyhypnidium riparioides (Hedw.) Dixon, Anomodon viticulosis (Hedw.) Hook & Taylor, Polytrichostrum formosum (Hedw.) G.L.Sm., Plasteurhynchium meridionale (Schimp.) M. Fleish. and Ctenidium molluscum (Hedw.) Mitt. were tested against Bacillus cereus, Bacillus subtilis, Micrococcus luteus, Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Enterobacter aerogenes, Proteus vulgaris, Proteus mirabilis, Pseudomonas aeruginosa, Pseudomonas putida, Salmonella typhimurium, Salmonella typhi, Neisseria gonorhoeae, Candida albicans, Rhodotorula rubra, Kluyveromyces fragilis, Kluyveromyces marxianus and Debaryomyces hansenii by disc diffusion method. The extracts of the plants especially P meridionale and A. viticulosis were active on all tested microorganisms.Öğe Evaluation of antimicrobial activity of two endemic Scrophulariaceae members(Asian Journal Of Chemistry, 2008) Dulger, Basaran; Hacioglu, NurcihanThe methanol extracts obtained from endemic Scrophulariaceae members Verbascum vacillans Murb. and Scrophularia depauperata Boiss. have been investigated for their antimicrobial activity. Antimicrobial activity was determined with Escherichia coli ATCC 11230, Stalphylococcus aureus ATCC 6538P, Klebsiella pneumoniae UC 57, Pseudomonas aeruginosa ATCC 27853, Proteus vulgaris ATCC 8427, Bacillus cereus ATCC 7064, Mycobacterium smegmatis CCM 2067, Listeria monocyogenes ATCC 15313, Micrococcus luteus CCM 169, Candida albicans ATCC 10231, Rhodotorula rubra DSM 70403. Cryptococcus neoformans ATCC 32308 and Kluyveromyces fragilis ATCC 8608 by disk diffusion method. While the extract of V vacillans has strong antimicrobial activity against the Gram-positive bacteria and yeast cultures, the other plant has weak activity against the test microorganisms used in this study.Öğe Monthly variation of some physico-chemical and microbiological parameters in Biga Stream (Biga, Canakkale, Turkey)(Academic Journals, 2009) Hacioglu, Nurcihan; Dulger, BasaranWater samples were collected from three different sites of the Biga Stream (Canakkale, Turkey) in the months of October 2007 - September 2008 for the analyzing of some physico-chemical and microbiological parameters of the stream. In the present investigation, the mean average value (mean +/- SD) of the stream temperature, dissolved oxygen (DO), biochemical oxygen demand (BOD(5)), pH, electrical conductivity (EC), total coliform (TC) and faecal coliform (FC) were noted as 15.533 +/- 0.199 degrees C, 8.332 +/- 0.253 mg/L, 136.60 +/- 2.51 mg/L, 7.5078 +/- 0.0427, 869.93 +/- 3.72 mu S/cm, 39381 +/- 7952 MPN/100 mL and 42500 +/- 7072 MPN/100 mL, respectively. Based on results of comparison of data with WPCR, it is seen that waters of Biga Stream at the sites 1, 2 and 3 belonged to Class 4, for parameter BOD5 and faecal coliform and belonged to Class 3 for parameter total coliform. We conclude that there is a great potential risk of infection of waters from the Biga Stream.Öğe Monthly variation of some physico-chemical and microbiological parameters in Saricay Stream (Canakkale, Turkey)(2010) Hacioglu, Nurcihan; Dulger, BasaranWater samples were collected from three different sites of the Saricay Stream (Canakkale, Turkey) in the months of October 2007 - September 2008 for the analyzing of some physico-chemical and microbiological parameters of the stream. In the present investigation, the mean average value (mean ± SD) of the stream temperature, dissolved oxygen (DO), biochemical oxygen demand (BOD 5), pH, electrical conductivity (EC), total coliform (TC) and faecal conform (FC) were noted as 17.737 ± 0.199 °C, 7.117 ± 0.650 mg/L, 170.4 ± 50.3 mg/L, 7.7018 ± 0.0325, 18.50 ± 2.33 ?S/cm, 46461 ± 10311 MPN/100 mL and 33103 ± 5863 MPN/100 mL, respectively. Based on results of comparison of data with WPCR, it is seen that waters of Saricay Stream at the sites 1, 2 and 3 belonged to Class 4, for parameter BOD 5 and faecal coliform and belonged to Class 3 for parameter total coliform. We conclude that there is a great potential risk of infection of waters from the Saricay Stream. © by PSP Volume 19-No 5a. 2010.Öğe MONTHLY VARIATION OF SOME PHYSICO-CHEMICAL AND MICROBIOLOGICAL PARAMETERS IN SARICAY STREAM (CANAKKALE, TURKEY)(Parlar Scientific Publications (P S P), 2010) Hacioglu, Nurcihan; Dulger, BasaranWater samples were collected from three different sites of the Saricay Stream (Canakkale, Turkey) in the months of October 2007 - September 2008 for the analyzing of some physico-chemical and microbiological parameters of the stream. In the present investigation, the mean average value (mean +/- SD) of the stream temperature, dissolved oxygen (DO), biochemical oxygen demand (BOD5), pH, electrical conductivity (EC), total coliform (TC) and faecal coliform (FC) were noted as 17.737 +/- 0.199 degrees C, 7.117 +/- 0.650 mg/L, 170.4 +/- 50.3 mg/L, 7.7018 +/- 0.0325, 18.50 +/- 2.33 mu S/cm, 46461 +/- 10311 MPN/100 mL and 33103 +/- 5863 MPN/100 mL, respectively. Based on results of comparison of data with WPCR, it is seen that waters of Saricay Stream at the sites 1, 2 and 3 belonged to Class 4, for parameter BOD5 and faecal coliform and belonged to Class 3 for parameter total coliform. We conclude that there is a great potential risk of infection of waters from the Saricay Stream.