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Öğe Antibiotic and Heavy Metal Resistant Bacteria Isolated from Aegean Sea Water and Sediment in Gulluk Bay, Turkey Quantifying the resistance of identified bacteria species with potential for environmental remediation applications(Johnson Matthey Publ Ltd Co, 2020) Altug, Gulsen; Cardak, Mine; Turetken, Pelin Saliha Ciftci; Kalkan, Samet; Gurun, SevanHeavy metal and antibiotic-resistant bacteria have potential for environmental bioremediation applications. Resistant bacteria were investigated in sediment and seawater samples taken from the Aegean Sea, Turkey, between 2011 and 2013. Bioindicator bacteria in seawater samples were tested using the membrane filtration technique. The spread plate technique and VITEK (R) 2 Compact 30 micro identification system were used for heterotrophic aerobic bacteria in the samples. The minimum inhibition concentration method was used for heavy metal-resistant bacteria. Antibiotic-resistant bacteria were tested using the disk diffusion method. All bacteria isolated from sediment samples showed 100% resistance to rifampicin, sulfonamide, tetracycline and ampicillin. 98% of isolates were resistant against nitrofurantoin and oxytetracycline. Higher antibiotic and heavy metal resistance was recorded in bacteria isolated from sediment than seawater samples. The highest levels of bacterial metal resistance were recorded against copper (58.3%), zinc (33.8%), lead (32.1%), chromium (31%) and iron (25.2%). The results show that antibiotic and heavy metal resistance in bacteria from sediment and seawater can be observed as responses to environmental influences including pollution in marine areas.Öğe First records and microgeographical variations of culturable heterotrophic bacteria in an inner sea (the Sea of Marmara) between the Mediterranean and the Black Sea, Turkey(Tubitak Scientific & Technological Research Council Turkey, 2013) Altug, Gulsen; Cardak, Mine; Ciftci, Pelin Saliha; Gurun, SevanThe microdiversity and composition of culturable heterotrophic aerobic bacteria were investigated in seawater samples taken from the Sea of Marmara (an important basin between the Mediterranean and the Black Sea) in different time periods throughout 2002 2010. The bacterial isolates were identified with the automated microidentification system VITEK 2 Compact 30 (bioMerieux, France). The compositions of identified bacteria according to their exposure to environmental factors in the areas from which they were isolated were compared. The primary hydrographic parameters (temperature, salinity, and dissolved oxygen) were recorded at the sampling station. The highest heterotrophic aerobic bacteria abundance was found in the coastal stations. It was possible to isolate enteric bacteria species from the lower and upper stratification of various marine localities, which possessed salinity values between 24.0 psu and 39.2 psu during the study. Six bacterial classes were determined: Gammaproteobacteria (49%), Bacilli (34%), Alphaproteobacteria (9.09%), Betaproteobacteria (3.03%), Flavobacteria (3.03%), and Actinobacteria (3.03%). This study increases the knowledge of the composition and biochemical response of bacteria isolated from eutrophic and oligotrophic areas. Twenty-three bacteria species belonging to 16 families are reported in this study as the first records for the Sea of Marmara.Öğe The occurrence of pathogenic bacteria in some ships' ballast water incoming from various marine regions to the Sea of Marmara, Turkey(Elsevier Sci Ltd, 2012) Altug, Gulsen; Gurun, Sevan; Cardak, Mine; Ciftci, Pelin S.; Kalkan, SametThe composition and frequency of antibiotic resistance of pathogenic bacteria, the abundance of heterotrophic aerobic bacteria (HPC) and possible in-situ use of chromogenic agar were investigated in the ships' ballast water coming from different regions of the world to the Sea of Marmara. Turkey for the first time. The samples that were taken from 21 unit ships coming from various marine environments of the Southern China Sea, the Atlantic Ocean, the Mediterranean and the Black Sea to the Sea of Marmara, Turkey in 2009 and 2010 were tested. 38 bacteria species, 27 of them pathogenic bacteria belonging to 17 familia, were detected. Vibrio cholera was not detected in the samples. However, the presence of a high number of HPC, including a cocktail of pathogenic bacteria showed that the ships carry a potential risk for the Sea of Marmara. (C) 2012 Elsevier Ltd. All rights reserved.