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    Effects of dietary diludine supplementation on growth, proximate composition, muscle and texture structure of rainbow trout juveniles
    (Iranian Fisheries Science Research Inst-Ifsri, 2018) Hisar, O.; Karaoglu, M. M.; Hisar, S. A.; Yanik, T.; Gurkan, M.; Kaya, H.
    A feeding trial which lasted for eight weeks was conducted to investigate the effects of diludine, a growth promoter, on feed efficiency, muscle structure and proximate composition of juvenile rainbow trout. Diludine was added at 0.0(D-0) 0.2(D-1), 0.5(D-2) and 1(D-3) g kg(-1) to a casein-based diet, and every diet was given to the triplicated groups of juvenile rainbow trout. At the end of experiment, it was determined that a significant improvability existed for both growth and feed utilization in fish fed diets supplemented with diludine (p<0.05). Similarly, different concentrations of diludine affected the densitometric quantification of myofibrillar proteins in fish muscle according to results obtained by Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The mean value of fiber diameters significantly increased in skeletal muscle with increasing concentrations of diludine. The histological results also showed hypertrophic adipocytes in skeletal muscle of fish fed D-2 and D-3 diets. The lowest elasticity values were observed in fish fed the control diet while those fed D-3 diet had highest elasticity values. On the other hand, no differences were found between fish fed experimental diets in terms of survival rate and all fish exhibited similar proximate composition for protein, lipid, moisture and ash. Consequently, it may be suggested that dietary diludine supplementaion up to 1 g kg(-1) concentration in the diets have positive impacts on growth of rainbow trout juvenils and the better growth in the fish fed with diludine supplements could be arise from muscle characteristics, in particular changes in fibres than proximate composition of the muscles.
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    Influence of Tribulus terrestris extract on the survival and histopathology of Oreochromis mossambicus (Peters, 1852) fry before and after Streptococcus iniae infection
    (Wiley, 2014) Yilmaz, S.; Ergun, S.; Kaya, H.; Gurkan, M.
    The aim of this study was to determine the effects of Tribulus terrestris extract (TT) on growth performance, disease resistance and histopathological changes in intestine and liver tissues of Oreochromis mossambicus (Peters, 1852) first-feeding fry before and after exposure to Streptococcus iniae. Five iso-nitrogenous and isocaloric diets were formulated to contain 0 (control), 200, 400, 600, and 800 mg kg(-1) TT. After feeding for 45 days, fish were infected with S. iniae and mortalities recorded. Final weight, weight gain and SGR of tilapia fry fed the 400 mg kg(-1) TT diet were significantly greater than that of control diet. In the challenge experiment, the best survival rate was obtained with 400 mg kg(-1) TT supplementation. Infection by S. iniae appeared to have a negative effect on histopathological findings and outcome than did TT-800 used alone. However, administration of TT (200 or 400 mg extract kg(-1)) resulted in overall improvement in the intestine and liver histopathology, emphasizing the protective potential of TT. The present study suggests the protective potential of TT in alleviating intestinal and hepatic damage that can occur after a S. iniae infection. It was concluded that 400 mg kg(-1) TT can enhance growth and disease resistance during first-feeding of O. mossambicus fry. This suggests that TT may be an alternative to antibiotics in controlling streptococcal disease in tilapia culture.