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    Öğe
    Protective Effects of Melatonin against Chronic Sodium Nitrite Exposure in Rats
    (2019) Uslu, Hamit; Uslu, Gözde Atila; Adalı, Yasemen; Makav, Mustafa; Gelen, Volkan
    In this study, anti-inflammatory effects melatonin (Mel) on liver and kidney damage induced by sodium nitrite (NaNO2) used as a food additive were investigated. The study groups were control group (C), NaNO2 group (NaNO2) and melatonin + NaNO2 group (Mel + NaNO2). The first group received dimethyl sulfoxide (DMSO) and the second and third groups received NaNO2 orally for twelve weeks. The third group received melatonin 2 hours before the administration of NaNO2. Administration of NaNO2 (80 mg/kg/day) for 12 weeks orally to rats increased serum aspartate aminotransferase (AST), alanine aminotransferase (ALT) (P<0.001) and urea (P<0.01) levels. Interleukin 1-alpha (IL-1?) (P<0.05) and tumor necrosis factor alpha (TNF-?) (P<0.01, P<0.001 respectively) levels were found to be increased in NaNO2 group in liver and kidney homogenates. It was also determined that IL-6 (P<0.001) levels were increased in kidney tissue. On the other hand, it was also found that there was a decrease at the levels of serum AST (P<0.001), ALT, urea (P<0.05), liver IL-1?, TNF-? (P<0.01), and kidney TNF-?, IL-6 (P<0.05) in group given melatonin (500 ?g/kg/day) 2 hours before NaNO2. In addition, it was observed that there was less liver and kidney damage than NaNO2 group in the pathological examinations Mel + NaNO2 applied group. The present data demonstrate that melatonin administration has visible modulatory effects and can eliminate inflammation; moreover, it can prevent the increase in biochemical markers caused by chronic sodium nitrite administration
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    Öğe
    Therapeutic effect of Tarantula cubensis extract on indomethacin induced gastric ulcers in rats
    (Chulalongkorn Univ, 2020) Makav, Mustafa; Gelen, Volkan; Gedikli, Semin; Atila Uslu, Gozde; Uslu, Hamit; Eroglu, Huseyin Avni
    Non-steroid anti-inflammatory drugs (NSAIDs) have widely been used in patients but side-effects associated with NSAID are also common. Tarantula cubensis extract (TCE) is an antiphlogistic, demarcative, homeopathic and necrotizing drug with wound healing effects in farm animals. However, the therapeutic effects of TCE on gastric ulcers has not been investigated. The aim of this study was to investigate the gastroprotective effects of TCE against indomethacin-induced gastric injury in rats. A total of 28 female, Wistar albino rats were divided into four groups: (i) the control group, (ii) the indomethacin (Indo) group, (iii) the TCE + Indo group, and (iv) the ranitidine + Indo group. We measured total antioxidant capacity, total oxidant capacity and oxidative stress index in the blood serum. We also measured tumor necrosis factor-alpha (TNF-alpha), interleukin-1 beta (IL-1 beta), IL-6, IL-8, and cyclooxygenase-2 (COX-2) activity level in the stomach tissue of rats. Finally, histopathology of the tissue of all groups was assessed. Immunostaining of 8-hydroxydeoxyguanosine (8-OHdG) and nuclear factor kappa B (NF-kB) was performed in the gastric tissue of all animals studied. Application of the TCE significantly decreased IL-1 beta and IL-6 levels were markedly lower than ranitidine. Thus, notable Indo-induced alterations in cytokines (IL-1 beta, IL-6) in rat gastric tissue were alleviated by TCE. Moreover, the gastric mucosal tissues of the TCE treated group exhibited a regular and uninterrupted mucosal layer and epithelial layer. Immunohistochemical results revealed that Indo increased 8-OHdG and NF-kB expression when compared to the control groups and treatment by TCE decreased the expression. The results obtained showed that the group receiving TCE exhibited lower gastric erosion and better efficiency than with ranitidine. These results have demonstrated that TCE provides a therapeutic effect in Indo-induced gastric ulcers in rats.