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    Investigation of Lycium barbarum Effects on Ovarium Damage Exposed to N-Methyl-N-Nitrourea
    (Springer, 2025) Eroglu, Hueseyin Avni; Buyuk, Basak; Aydeger, Cemre; Demir, Ufuk; Makav, Mustafa
    N-Methyl-N-nitrourea (MNU), is one of the N-nitroso compounds that people are commonly exposed to in various exogenous ways such as diet, tobacco smoke, cosmetics, household goods, indoor air, occupational exposure, etc. It is known that the compound damages organs and tissues in the body, and one of the affected structures is the ovarium. Lycium barbarum, used in medication in ancient China, has become more popular in recent years. Previous studies have mentioned that polysaccharide ingredients are the most bioactive parts of Lycium barbarum. Therefore, this study aims to investigate the effects of Lycium barbarum polysaccharides (LBP) on ovarian tissue damage exposed to MNU. In accordance with this purpose, a total of 12 21-day-old female Wistar Albino rats were divided into two groups: sham and treatment. The animals were administrated with 50 mg/kg MNU (i.p.) on days 21, 28, 35, and 42. The treatment group was treated with 800 mg/kg LBP via intragastric for 30 days. At the end of the study, animals were sacrificed under general anaesthesia, and the ovarian tissues were harvested. Shrinkage of follicles, lytic oocytes, and disintegrated stroma parameters were evaluated histopathologically. The genetic assessment of the Bcl-2, Bax, Bcl-2/Bax ratio, Caspase 3, p53, and p27 expression levels were determined. The histopathological parameters between the groups were statistically significant (for all parameters p < 0.001). Moreover, the Bcl-2, Bcl-2/Bax ratio, and p53 relative fold changes were significantly increased in the treatment group (p < 0.001, p = 0.045, and p = 0.011, respectively). The results revealed that LBP has ameliorative effects on MNU-induced ovarian tissue damage. Further studies are required to clarify the main underlying mechanisms.
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    Investigation of the Effects of Dimethyl Sulfoxide in Experimental Gout with Comparison of Dexamethasone and Indomethacin
    (Springer, 2024) Aydeger, Cemre; Adali, Yasemen; Makav, Mustafa; Eroglu, Hueseyin Avni
    Gout arthritis is an inflammatory arthritis characterized by increased serum uric acid and accumulation of monosodium urate (MSU) crystals in soft tissues. The treatment for gout arthritis is centered on reducing uric acid agents with long-term and anti-inflammatory agents during attack times. In recent studies, it is noteworthy that Indomethacin and Dexamethasone have positive effects in the treatment of gout. Dimethyl sulfoxide (DMSO) is a lipophilic solvent and has an anti-inflammatory effect at appropriate doses. Based on this information, for this study, the effects of these three agents were investigated in rats using a gut model to compare their efficacy. In the study, a total of 48 female 3-4-month rats were divided equally into 8 groups: Control, Indomethacin, DMSO, Dexamethasone, Gout, Gout+Indomethacin, Gout+DMSO, Gout +Dexamethasone. During the eight-week study, a gout arthritis model was used that included 10 mg MSU given intra-articularly in the right foot. Indomethacin 12.5 mg/kg intragastric, DMSO 0.1 ml intraperitoneally and dexamethasone 0.2 mg/kg were administered subcutaneously to the related groups once a day for seven days. At the end of the study, collected articular tissues were stained with haematoxylin and eosin after the fixation and decalcification processes were done. The findings obtained showed that inflammation was reduced in treatment groups compared to the Control groups (all p values 0.002). Also, synovial proliferation was remarkably decreased in the Gout+Dexamethasone group compared to the Gout group (p = 0.019). As a result of these findings, although the three agents all reduced inflammation in gout arthritis, DMSO was shown to be more advantageous due to its having fewer side-effects.
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    Selenium or ozone: Effects on liver injury caused by experimental iron overload
    (Pergamon-Elsevier Science Ltd, 2020) Guevendi, Guelname Frndrk; Eroglu, Hueseyin Avni; Makav, Mustafa; Guvendi, Buelent; Adalre, Yasemen
    Aims: Iron is an important metal ion as a biocatalyst on the other hand iron overload causes various diseases. Iron overload can result in fibrosis and hepatocellular carcinoma with various pathophysiological mechanisms, including oxidative damage in the liver. Therefore; in this study the effects of ozone and selenium-whose antioxidant properties are known- were evaluated in liver injury induced by iron overload. Materials and methods: Iron overload model was provided by intraperitoneal administration of 88 mg/kg iron dextrate for 4 weeks. After iron dextran administration, ozone and selenium administrations were made for 3 weeks. From the obtained blood and tissue samples total oxidant status (TOS) and total antioxidant status (TAS) were determined and histopathological examination was performed in liver tissue samples. Key findings: In rats with iron overload, the lowest mean serum TOS was observed in the selenium administration group. The highest tissue TOS means and the lowest tissue TAS means were determined in the group in which ozone and selenium were administrated together. When histopathological data were evaluated, the presence of increased apoptosis in the ozone group compared to the iron group (p = 0.019) and selenium group (p= 0.019) was noted. Similarly, increased periportal inflammation (p = 0.001) and fibrosis (p = 0.005) were observed in the ozone group compared to the selenium group. Significance: In iron-induced liver damage, ozone was thought to be effective by decreasing ROS, but contrary to expectations, it was observed that it may negatively affect the picture by showing synergistic effect. However, the effects of selenium on both serum and tissue levels are promising.