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Öğe Can amniotic fluid be an alternative organ preservation solution for cold renal storage?(Asociacion Regional de Dialisi y Transplantes Renales de Capital Federal y Provincia de Buenos Aires, 2020) Büyük, Başak; Demirci, Tuba; Adalı, Yasemen; Eroğlu, Hüseyin AvniIntroduction: Kidney-transplantation is a lifesaving treatment option for patients with chronic renal failure. Preserving the viability of the organ from the removal of the organ until transplantation into the recipient is one of the most essential factors affecting postransplant success. Kidney tissue is exposed to ischemia following removal of the organ from the donor, initiating some cellular events. Amniotic fluid (AF) was previously reported as a preservation solution for the liver, but not for the kidney yet. The aim of this study is to investigate the effectiveness of AF as a preserving solution for rat kidneys compared with the University of Wisconsin (UW) and Histidine-Tryptophan- Ketoglutarate (HTK), which are reported to be the most commonly used and preferred preserving solutions. Methods: Forty male Wistar albino rats were used in this study in four experimental groups. Group 1: Ringer Lactate (RL, Control) group, Group 2: HTK group, Group 3: UW group, and Group 4: AF group. A midline incision was performed, and the renal artery was isolated under ketamine and xylazine anesthesia. Solutions relevant for groups (cooled to + 4°C) were used for kidney perfusion. Nephrectomy was applied, and the removed kidneys were placed into + 4°C standard organ storage solution and stored at + 4° C for 12 hours. After 12 hours of storage, samples from the kidney tissues were fixed in 10% neutral buffered formalin. Histopathological, immunohistochemistry evaluation and apoptosis detection via TUNEL method were performed. Results: The results of the AF group were close to those of the UW and HTK groups. Tubular necrosis and vacuolization were high in the RL solution group when compared to the other experimental groups. Immunohistochemistry staining for all three markers (TNF-alpha, IL-18, and iNOS) was decreased in the amniotic fluid group, similar to the UW and HTK groups. Also, the number of apoptotic cells was decreased in the AF group compared to control. Conclusions: UW, HTK, and AF had similar and higher protective effects compared to the RL solution. Thus, AF may be used as an inexpensive and readily available alternative natural tissue preservation solution. © 2020, Asociacion Regional de Dialisi y Transplantes Renales de Capital Federal y Provincia de Buenos Aires. All rights reserved.Öğe Effect of Ganoderma lucidum polysaccharides on oxidative damage in liver of STZ-diabetic rats(Scientific Publishers of India, 2018) Eroğlu, Hüseyin Avni; Beytut, EbruIn this study, the effects of exogenic application of Ganoderma lucidum polysaccharide on oxidative stress and hyperlipidemia was determined in diabetics rats. The study included 60 Wistar albino rats aged 4-5 months. Rats were divided into 6 equal groups. The groups were control, Ganoderma lucidum polysaccharide, diabetic, diabetic+60 mg/kg polysaccharides, diabetic+120 mg/kg polysaccharides and diabetic+10 mg/kg polysaccharide. Diabetes was induced by intraperitoneal injection of streptozocine at the dose rate of 50 mg/kg. The rats were fed ad-libitum throughout the experiment. At the end of the study, we took some samples out of total oxidant and total antioxidant levels and measured them with the spectrophotometer; thereafter we used the auto-analyser to measure the levels of total cholesterol, triglycerides, HDL, LDL, VLDL cholesterol and pancreatic amylase. We examined the blood of diabetic fasting rats and the effect was that their glucose level was increased significantly. (p<0.05), nonetheless the dose of 60, 120 and 180 mg/kg of Ganoderma lucidum polysaccharides did not reduce the blood glucose level (p>0.05). With the diabetic rats, the dose of 60, 120 and 180 mg/kg of Ganoderma lucidum polysaccharides rides effected in the increase of serum levels of total cholesterol, triglycerides, VLDL, and HDL cholesterol levels, although it was not a significant change (p>0.05), instead, we observed a statistically significant decrease of LDL cholesterol level (p<0.05). According to this study we concluded that polysaccharides effects in the decrease of hyperlipidaemia with regard to diabetes. During the study we applied Ganoderma lucidum polysaccharides exogenously (60, 120, 180 mg/kg) and it did not affect the level of total antioxidant in diabetes significantly (p>0.05), however with a dose of 180 mg/kg the total oxidant level in pancreas and liver tissues was reduced significantly and has been considered as crucial fact. The collected data supported our view on histopathologic examination. Based on all the information, we used one type of fungus Ganoderma lucidum polysaccharides which did not influence the blood glucose level in diabetes, whereas with regard to LDL and total oxidants it played a significant role in reducing its levels. © 2018, Scientific Publishers of India. All rights reserved.Öğe EFFECTS OF CORTICOSTEROID AND ANTIHISTAMINIC INJECTIONS ON COLD-INDUCED STRESS ON RAT BLADDER TISSUE: AN EXPERIMENTAL STUDY(2020) Eroğlu, Hüseyin Avni; Büyük, Başak; Adalı, Yasemen; Demir, AslanObjective: Cold is a stress-inducing factor that can cause changes in bladder function in various ways. The present study is intended to investigate the effects of corticosteroid and antihistaminic treatment on acutely and chronically induced cold stress. Material and Methods: Forty-two female rats were randomly divided into seven groups as follows: Control group; Acute cold-stress (ACS) group; ACS+ corticosteroid (CORT) group; ACS+CORT + Antihistaminic group; Cronic cold-stres (CCS) group; CSS+CORT group; CSS+CORT+Antihistaminic group. On the 15th day after these treatments, bladders of the rats were harvested for histopathological examinations under general anesthesia and fixed with 10% neutral buffered formaline. Hematoxylin and eosin and toluidine blue stainings were performed. Results: The comparison based on mast cell count yielded the highest value in the CCS group in comparison to the control group. The lowest value was harvested in the CCS + CORT group. The comparison between the CCS groups revealed the highest polymorphonuclear leucocyte (PNL) values in the lamina propria in the CCS group, whereas the CORT and CORT + Antihistaminic treatments were found to have significantly decreased the PNL values in the lamina propria. While the PNL counts in the epithelium were high in the ACS and CCS groups, the results in the ACS and CCS groups that were treated with CORT and/or antihistaminic were revealed to be similar with those in the control group. It was discovered that antihistaminic injection in addition to CORT decreased the lymphocyte counts in epithelium in CCS more efficiently than CORT alone did. Conclusion: The present research revealed that corticosteroid treatment reduced inflammatory cell infiltration and decreased mast cell count. A more evident amelioration was observed particularly in chronic cold stress.Öğe EFFECTS OF DIFFERENT DOSES OF SHIITAKE MUSHROOM (LENTINUS EDODES) ON SPERM PARAMETERS AND TESTICULAR TISSUE IN RATS: AN EXPERIMENTAL STUDY(Pakistan Agricultural Scientists Forum, 2023) Büyük, Başak; Demir, Neslihan; Eroğlu, Hüseyin Avni; Öztopuz, Rahime ÖzlemLentinus edodes has been cultivated and used as food and medicine in far eastern countries.The present study aimed to reveal whether L.edodes that is used for different purposes has a toxic effect on the male genital system and to investigate its relationship with male infertility by showing its effects on sperm parameters. Twenty-four male Wistar rats were randomly divided into three groups as Control (C), Low-dose (LD) and High-dose (HD). Rats in Control were administered saline physiological once a day for 7 days. The LD and HD groups received 100 and 400mg/kg of L.edodes extract, respectively, once daily for 7 days. At the end of 7 days of administration of treatments, semen analysis, micronucleus analysis, gene expression levels and testicular Johnsen scoring was performed. Comparisons between groups without normal distribution were analyzed using Kruskal-Wallis variance analysis and those between groups with normal distribution were analyzed using one-way analysis of variance, followed by Tukey's test. P values of <0.05 were considered statistically significant. It is demonstrated that both low and high doses of extract significantly reduced spermatogenesis in seminiferous tubules found in the testicles of rats. In addition, high-dose administration resulted in decreased total sperm counts, whereas the progressive and non-progressive sperm motility counts were significantly decreased in both experimental groups. The gene expression levels of Casp-3 and TNF-alpha were increased in both the LD and HD groups compared with that in the C. Oral administration of low and high doses of L.edodes led to reduced spermatogenesis in the testicles, tissue damage to the testicle and decreased motile and total sperm counts.Öğe Effects of Ozone Therapy on Chronic Arsenic Poisoning in Rats(Springer, 2023) Büyük, Başak; Aydeğer, Cemre; Öztopuz, Özlem; Ovalı, Mehmet Akif; Makav, Mustafa; Eroğlu, Hüseyin AvniArsenic (As) is a toxic metalloid that affects many organs through drinking water. This study aims to examine the efficacy of ozone therapy on chronic arsenic toxicity. Twenty-four male Wistar albino rats were housed in individual cages and grouped as control, As, O3, and As + O3. As was applied by adding 5 mg/kg/day in drinking water for 60 days. Ozone therapy was applied at 0.5 mg/kg/day (i.p.) O3 in the last 5 days of the experimental period. Tissues were harvested and analyzed for histopathological injury and apoptotic markers. There was no significant difference between the As + O3 and O3 groups (p = 0.186 and p = 0.599) for light microscopic criteria: inflammatory cell infiltration and hydropic degeneration in liver tissue. In TUNEL assessments, similar outcomes were obtained in the control and As + O3 groups. A statistically significant increase was observed in p53 and Caspase 3 (Casp-3) expression levels in the As group compared to the O3 and As + O3 groups. There was no significant difference between the As + O3 and O3 groups on peritubular hemorrhage and desquamation parameters in kidneys (p = 0.147 and p = 0.094). The KIM-1 expression level was significantly increased in the As group compared to the As + O3 group (p = 0.01), and the Casp-3 expression level was not significantly changed in the O3 group compared to the As + O3 group (p = 0.59). In conclusion, it is determined that ozone therapy has ameliorative effects on the microscopic injury of liver and kidney tissues. In addition to microscopic improvement, KIM-1 gene expression levels were ameliorated in the kidneys. The apoptotic cell counts and the Casp-3 and p53 gene expression levels were decreased by O3 administration. Thus, ozone therapy can be a treatment choice for As toxicity.Öğe Evaluation of Blood and Cerebrospinal Fluid Biochemistry, Cytology and Haematological Parameters in Head-and-Eye Form of Malignant Catarrhal Fever in Cattle(2023) Uzlu, Erdoğan; Erkılıç, Ekin Emre; Adalı, Yasemen; Öğün, Metin; Şahna, Kezban Can; Eryeğen, Nilhan; Eroğlu, Hüseyin AvniIn this study, it was aimed to examine the biochemical changes, hematological changes and cerebrospinal fluid (CSF) cytology and blood serum of cattle with head-eye form of Malignant Catarrhal Fever (MCF). For this pur- pose, 22 cattle diagnosed with \"head-eye form\" of MCF and clinically healthy 10 cattle were evaluated. Blood and cere- brospinal fluid (CSF) were collected from all cattle. In sera, AST, urea, glucose, CK (P<0.05), LDH levels (P<0.01) were found be high, ALT, ALP, cholesterol (P<0.05), Ca, total protein (P<0.01) and Mg, albumine and Fe levels (P<0.001) were found to be low in MCF group when compared to the control group. In CSF, Ca (P<0.01) and total protein levels (P<0.001) were found high glucose level (P<0.05) was found low in MCF group when compared to the control group. In haematology, some parameters were determined to be different between the groups. In cytological results of CSF in MCF group, polymorphonuclear leucocytes, lymphocytes, erytrocytes, macrophages and plasma cells were determined. In conclusion, since there were a limited number of studies examining biochemical, cytologic and hematological results of MCF especially in CSF, the results from our study were thought to be important for future stud- ies in which viral diseases affects the nervous system of cattles.Öğe Experimental intravaginal and intrauterine endometritis model: which model is more useful?(2022) Makav, Mustafa; Kuru, Mushap; Beşeren, Hatice; Adalı, Yasemen; Coşkun, Mustafa Reha; Eroğlu, Hüseyin AvniThis study aims to compare the newly created intravaginal endometritis model (IVM) with the intrauterine endometritis model (IUM). E. coli infusion was used as intravaginally for IVM and intrauterinally for IUM model. The animals were exeuted on the 7th day. Histopathological and biochemical analyses [malondialdehyde (MDA), glutathione (GSH), Endocan, Endoglin] were performed. A significant inflammation was determined in IVM and IUM compared to the control. A significant decrease in GSH and a significant increase in MDA and Endoglin were determined in IVM and IUM compared to the control. There was a statistically significant increase in the IUM and a numerical increase in the IVM compared to the control. Endometritis was determined by histopathological and biochemical analyses in both IUM and IVM model. It is suggested that intravaginal administration, which is easier to perform, can be used in experimental endometritis model studies.Öğe Fine Needle Aspiration Cytology (FNAC) vs. Tru-cut Biopsy in Liver with Iron Overload: An Experimental Study(2023) Adalı, Yasemen; Eroğlu, Hüseyin Avni; Makav, Mustafa; Karayol, Sunay Sibel; Güvendi, Gülname Fındık; Gök, MustafaObjective: Fine needle aspiration cytology (FNAC) is safer, simpler, faster and cost effective, compared to tru-cut biopsy, and patient compliance is generally better. There is not much data on the use of FNAC, which is used in many organs and lesions, in parenchymal diseases in the liver. Therefore, in this study, the benefit of FNAC in showing iron deposition and injury of liver is evaluated. Methods: There were 2 control groups in the study. At the end of the study, FNAC and tru-cut biopsy samples were taken from these groups, where no administration was performed. FNAC and tru-cut biopsy procedures were also performed to the study groups after the iron overload was created by intraperitoneal iron dextran (88 mg/kg) administration for 28 days. Hepatocyte injury, inflammation and iron deposition were evaluated histopathologically in the preparations. The sensitivity, specificity, positive and negative predictivity and diagnostic accuracy of both methods were calculated based on the hepatectomy material. Results: In hepatocyte injury; sensitivity (60%), diagnostic accuracy (66.7%) and negative predictivity (50%) were found to be significantly higher in tru-cut group. It was observed that the methods were not superior to each other in the evaluation of inflammation, and FNAC almost approached tru-cut biopsy rates (100%) with a sensitivity of 83.3% and diagnostic accuracy in iron deposition. Conclusion: In iron-induced liver damage, the gold standard should be tissue examination, so tru-cut biopsy must be the first choice. However, based on the present study results, it is suggested that FNAC may be preferred over more invasive options in cases where tru-cut biopsy can not be used in terms of susceptibility to bleeding, situations where surgical accessibility is not available, patient orientation and cooperation is poor, or in presence of different accompanying diseases.Öğe Ozone vs Melatonin: The Therapeutic Effects In Alcoholic Liver Disease(2020) Eroğlu, Hüseyin Avni; Makav, Mustafa; Güvend, Gülname Fındık; Büyük, Başak; Adalı, YasemenBackground: Alcoholic liver disease (ALD) is a worldwide health problem.Ozone and melatonin areagents that are widely used for their antioxidant properties in medical therapies.In this study, we aimedto evaluate the use of ozone and melatoninin ALD.Materials and Methods: Forty-eight rats were used, and ethanol, melatonin, and ozone wereadministrated. Histopathological evaluation was performed and activities of alanine aminotransferase(ALT), aspartate aminotransferase (AST), gamma glutamyl transferase (GGT) total antioxidant status(TAS), total oxidant status (TOS), and malondialdehyde (MDA)were determined in the blood samples,while TAS, TOS, and MDA parameters were measured in the liver tissue samples.Results: There were statistically significant differences between the studied groups concerning ALT,AST activities and TAS, TOS, and MDA levels. Vascular congestion, hepatocyte damage, periportalinflammation, and microvesicular steatosis were detected inthe alcohol-induced toxicity group.Conclusions: In the light of histopathological findings, we can claim that melatonin and ozoneadministrations are beneficial for alcohol-induced hepatotoxicity. However, ozone application issuperior to melatonin.Öğe Physiopathology of Wound Healing in Central Nervous System(Hacettepe University, Faculty of Pharmacy, 2023) Aydeğer, Cemre; Eroğlu, Hüseyin AvniWound healing refers to regeneration of damaged tissue under various circumstances and activation of specific physiologic mechanisms. A wound is an immediate onset of tissue damage, contusion, or degeneration. Wounds are common pathological actions of body. After wounds happened, they should lean on healing at specific processes. Wound healing contains 4 phases initially as follows: haemostasias, inflammation, proliferation, and remodelling. Novel studies demonstrated those 4 phases with dermis wound healing process. Nevertheless, every tissue has its own healing pattern. Especially central nervous system differs from dermis in healing process. Therefore, the physiopathological wound healing mechanisms of central nervous system have been referred under novel information in this review. Healing of central nervous system is a complicated process, and the mechanisms of healing are still lacking due to several bunch of studies. Thus, clarification of underlying mechanisms of healing in central nervous system is indispensable for the sufferers. © 2023, Hacettepe University, Faculty of Pharmacy. All rights reserved.Öğe Predictive value of inflammatory cell ratios in incidental thyroid papillary carcinoma(2019) Eroğlu, Hüseyin Avni; Karayol, Sunay Sibel; Güvendi, Bülent; Adalı, YasemenIntroduction: Due to the well-known relationship between inflammatory processes and carcinogenesis, the diagnostic and prognostic value of systemic inflammatorymarkers are at the focus of research. This study aimed to investigate the predictive value of inflammatory cell data obtained from complete blood count in incidentallydetected thyroid papillary carcinoma.Methods: A total of 100 cases were included in the study, consisting of histopathologically diagnosed multinodular hyperplasia (MNH) (N = 20), lymphocytic thyroiditis(LT) (n = 20), incidental thyroid papillary carcinoma (ITPC) (n = 20), incidental papillary carcinoma and lymphocytic thyroiditis (ITPCLT) (n = 20), and thyroid papillarycarcinoma (TPC) (n = 20). Neutrophil / lymphocyte ratio (NLR), platelet/ lymphocyte ratio (PLR), platelet/neutrophil ratio (PNR), lymphocyte/ monocyte ratio (LMR)and lymphocyte/ eosinophil ratio (LER) values were compared between the groups.Results: No significant difference was detected between the groups concerning the mean inflammatory cell and platelet values. However, statistical significance wasobserved between the ITPC and ITPCLT groups regarding the mean LMR and LER values (p=0.009 and p=0.037, respectively). Higher LMR was found in the ITPCgroup, while higher LER was seen in the ITPCLT group.Conclusions: We suggest that besides ratios such as NLR and PLR used as systemic inflammatory markers in carcinogenetic processes, the evaluation of LER and LMRmight be imperative as well. Therefore, these potential markers should be studied in bigger series of tumors, especially where inflammatory processes are involved in theetiologyÖğe Recuperative effect of estrogen on rotenone-induced experimental model of Parkinson’s disease in rats(Springer, 2021) Makav, Mustafa; Eroğlu, Hüseyin AvniParkinson’s disease (PD) is described as the loss of dopaminergic neurons located in the substantia nigra (SN) region of the brain and a progressive motor failure. Increased frequency of PD in women, especially after menopause, suggests the effect of estrogen. This view has been supported with empirical studies. Therefore, the effect of estrogen in an experimental model of Parkinson’s disease induced by rotenone was investigated. A total of 32 female Wistar Albino rats were randomly assigned to four groups (control group, ovariectomy group, Parkinson’s group, Parkinson’s + estrogen group). The Parkinson’s group received rotenone subcutanously at the dose of 2.5 mg/kg bw, on the 1st, 2nd, 3rd 4th, 6th, 9th, 12th, 15th, 18th, and 21st days animals in the Parkinson’s + estrogen group received retonon as in the Parkinson’s group and was additionally subcutaneously given estrogen (implant containing 0.5 mg 17 β-estradiol lasting for 21 days). The rats were subjected to rotarod, pole, and swimming tests at the end of the experiment for comparison of their motor activities, and then, histopathological and biochemical analyses were performed on the tissues that were extracted. The rotarod results revealed that Parkinson’s group had the shortest time (32.33 ± 3.98 sn) than the groups of control (92.50 ± 12.60 s) ovariectomy (71.42 ± 10.58 s), and Parkinson’s + estrogen (71.37 ± 9.26 s). The results of pole disclosed that return and landing time prolonged for Parkinson’s group when compared with other groups (return time for control 2.98 ± 0.38 s, ovariectomy 3.02 ± 0.75 s, Parkinson 5.91 ± 0.33 s, Parkinson’s + estrogen 3.48 ± 0.42 s and landing time for control 5.30 ± 0.59 s, ovariectomy 5.45 ± 0.73 s, Parkinson 9.80 ± 0.90 s, Parkinson’s + estrogen 5.37 ± 1.02 s). Parkinson’s group had longest (90.71 ± 12.56 s) swimming time to reach the target when compared with control (33.16 ± 8.68 s), ovariectomy (47.37 ± 12.19 s), and Parkinson’s + estrogen (49.82 ± 5.78 s). Histopathological examination indicated a significant difference in tyrosine hydroxylase-stained cells (dopaminergic neurons and dopamine) between the Parkinson’s + estrogen group and the Parkinson’s group. The biochemical analyses of Caspas-3 activation in SN and striatum (STR) was significantly different between the Parkinson’s + estrogen group and the Parkinson’s group, but this difference was not observed in STR while evaluating Bcl-2. The results of this study suggested that estrogen may have a recuperative effect on PD.Öğe Sleeve Gastrektomilerde Histopatolojik Bulgularımız: 63 Vakanın Analizi(E-Journal of New World Sciences Academy, 2019) Adalı, Yasemen; Toyran, Tuğba; Eroğlu, Hüseyin Avni; Binnetoglu, Kenan; Akkan, Selda Aksu; Güvendi, Bülent; Güvendi, Gülname FındıkSleeve gastrektomi gelişmiş ve gelişmekte olan ülkelerde önemli bir sağlık sorunu olan obezite tedavisinde kullanılan cerrahi yöntemlerden biridir. Biz çalışmamızda morbid obezite nedeni ile yapılan sleeve gastrektomi materyallerindeki histopatolojik bulguları ortaya koymayı amaçladık. Çalışmaya 2013-2017 haziran ayları arasında üniversitemiz genel cerrahi kliniğinde sleeve gastrektomi operasyonu yapılan 63 morbid obez olgu dahil edildi. Olgular gastrit, intestinal metaplazi, Helicobacter pylori, atrofi ve diğer histopatolojik bulgular açısından 3 farklı patolog tarafından tekrar değerlendirildi. Çalışmaya dahil edilen olguların %71.4'ü kadın, %28.6 erkek olup yaş ortalamaları 35.37+9.429 saptandı. Olguların %77.8'inde kronik gastrit, %22.2'sinde aktif kronik gastrit gözlendi. İntestinal metaplazi %23.8 olguda, Helicobacter pylori varlığı %93.7 olguda ve atrofi varlığı %4.8 olguda izlendi. Çalışmamızda tüm vakalarda kronik veya aktif kronik gastrit izlenmesi yanı sıra Helicobacter pylori varlığı literatürde bildirilenden daha yüksek bir oranda saptanmıştır.Öğe The Effect of Topically Applied Boric Acid on Ephrin-Eph Pathway in Wound Treatment: An Experimental Study(SAGE Publications, Early Access) Büyük, Başak; Aydeğer, Cemre; Adalı, Yasemen; Eroğlu, Hüseyin AvniBackground: Wound healing has a vital importance for the organism and various agents are used to accelerate wound healing. Although the effect of boron on wound healing is known, its mechanisms are not completely clear yet. In this study, the effect of boron in the Ephrin /Eph pathway will be evaluated. Methods: Forty adult female rats were used in the study. A full-thickness excisional wound model was created in all groups divided as Control, Fito, Boron and Plu groups. After the applications performed twice a day and lasting 7 days, skin tissues obtained and evaluated histopathological (inflammatory cell infiltration, oedema, and fibroblast proliferation density) and immunohistochemical (TNF-α, EphrinA1, EphrinB1, EphrinB2 and EphB4). Results: Inflammatory cell infiltration score was found to be higher in the Fito group compared to Boron group (p =.018). Fibroblast proliferation density was higher in Plu group than Boron group (p=.012). While TNF-α was lower in boron group than Plu (p =.027) and Fito (p =.016) groups, EphrinA1 was higher in Boron group than Plu group (p =.005). EphrinB1 expression was higher in Boron group compared to Plu (p= .015) and Fito (p =.015) groups, and the same difference was also observed in EphrinB2 (p values .000). Similarly, EphB4 immunoreactivity was higher in the Boron group compared to Plu (p=.000) and Fito (p =.002). Conclusion: One of the mechanisms of action of boron in wound healing is to increase EphrinB1, EphrinB2 and EphB4. Low TNF-α and histopathological findings indicate that boron limits extensive wound healing.Öğe The Effect of Turkish Coffee on Performance, Biochemical, and Physiological Parameters in Rabbits(2021) Makav, Mustafa; Ölmez, Mükremin; Eroğlu, Hüseyin Avni; Şahin, TarkanCoffee is one of the most widely consumed hot beverages in the world. Melanoidins, which are formed with brown color during coffee roasting, are reported to be a strong antioxidant. Discussions related to the more and less consumption of coffee still continue. This study investigated the effect of a low and high amount of coffee consumption on antioxidant parameters in this context. A total of 15 female New Zealand rabbits weighing 1500-2500 g were used in the study. The control group was fed as ad-libitum control with basal feed. 1% and 5% Turkish coffee were added to the feed of the 1% TC and 5% TC groups, respectively. Blood was collected once a week throughout the study. All animals were euthanized in accordance with ethical rules at the end of the study. Samples (plasma and tissue) were taken for the analyses. Glutathione (GSH) and malondialdehyde (MDA) analyses were performed on all samples. Plasma GSH values were observed to increase in the groups given TC according to the analyses. Plasma MDA values decreased in the TC groups. An increase was observed in liver and heart tissue in the TC group upon the evaluation of tissue GSH parameters. MDA values were found to decrease in lung, heart, and kidney tissues in TC groups. Additional effects were observed in plasma and tissues in the 5% TC group upon the evaluation of 1% TC and 5% TC groups. No statistical differences were found in performance parameters. It has been revealed as a result that TC is a strong antioxidant, and its effect increases in high consumption.Öğe The Effects of Thiopental on Cold Ischemic Injury in Renal Transplantation(Medical University of Plovdiv, 2023) Büyük, Başak; Adalı, Yasemen; Karakoç, Ebru; Eroğlu, Hüseyin Avni; Aydeğer, CemreIntroduction: One of the most important factors influencing post-transplant success in kidney transplantation is preserving the viability of the organ from removal to transfer into the recipient. Aim: This study aimed to reduce the energy requirement with thiopental doses administered before organ transplantation, and to increase the organ viability by minimizing the tissue damage during the cold ischemia process. Materials and methods: Twenty female Wistar albino rats were divided into two groups: control group (group C), and thiopental group (group T). In group C, a midline incision was performed, and the renal artery was isolated under ketamine and xylazine anesthesia. A standard organ storage solution (cooled to +4°C) was used for kidney perfusion. Nephrectomy was applied, and the removed kidneys were placed into +4°C standard organ storage solution and stored at +4°C for 12 hours. Animals in group T were subjected to the procedures explained above under 85 mg/kg thiopental sodium anesthesia. After 12-hour storage, samples from the kidney tissues were fixed in 10% neutral buffered formalin. Histopathological evaluation and apoptosis detection via TUNEL method were performed. Results: Tubular necrosis was more extensive in group C compared with that in group T and this difference was statistically significant. Similarly, vacuolization was widely observed in group C, and this increase was also statistically significant. For the ‘dilatation of Bowman’s space’ parameter, a significant decrease was observed in group T compared with group C. When the apoptotic index values of both groups were examined, it was seen that they were lower in group T than those in group C. This result was statistically significant. Conclusions: These data suggest that thiopental provides protection to the kidney tissue during the cold storage process. Thiopental has been shown to decrease the number of apoptotic cells in the kidney tissue when administered to the donor before organ transplantation, increasing the organ viability.Öğe The Influences of Metformin on Prostate in Terms of PSA Level and Prostate Volume(Urology and Nephrology Research Centre, 2021) Atalay, Eray; Demir, Aslan; Eroğlu, Hüseyin AvniPurpose: The effects of metformin on prostate volume and prostate-specific antigen (PSA) were investigated. Materials and Methods: We enrolled 384 newly diagnosed diabetes mellitus (DM) patients and 152 controls all of whom were >50 years into our prospective cross-sectional observational study. The first group contained patients receiving metformin only, the second group patients were taking a mixture of medications, including metformin plus other oral anti-diabetics, and the third was the control group. Before beginning treatment, body mass indices (BMI) of all cases were obtained. Prostate volumes were evaluated using transabdominal ultrasonography at the sixth and twelfth months. Insulin, glycosylated hemoglobin (HbA1C), insulin sensitivity index (ISI), insulin- rich growth factor (IGF-1), PSA, free PSA, and total testosterone levels were measured. Results: The differences in BMI between the first and third groups were statistically significant (P < 0.05). There were no statistical differences among the groups in terms of prostate volumes (P > 0.05). The differences between the groups for insulin, HbA1C, ISI, IGF-1 (somatomedin), PSA, free PSA, and total testosterone levels were not statistically significant (P > 0.05). Free PSA and total testosterone levels in groups 1 and 2 were not statistically different at the beginning of treatment and the sixth month (p >0.05), but within groups 1 and 2, only PSA levels were different at the start of the study until completion. No differences were seen in the third group. Conclusion: Metformin appears to cause a decrease in PSA levels. The mechanism and any effects on prostate tissue will be studied in future randomized, prospective studies.
