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    Is There Any Correlation Between The Elevated Plasma Levels and Gene Variations of Factor VIII in Turkish Thrombosis Patients?
    (Sage Publications Inc, 2011) Ay, Mustafa; Dolek, Bilgen; Erdem, Gunhan; Devecioglu, Omer; Gozukirmizi, Nermin
    We investigated factor VIII (FVIII) gene mutations in 20 thrombosis patients with high level of FVIII and 20 control healthy participants. Blood samples were used for the determination of FVIII levels using static timing analyze (STA) kits. Informed consent forms were collected from all participants. Factor VIII level was 237 +/- 46 IU/dL in patients group; however, it was 122 +/- 38 IU/dL in healthy control participants. Isolated genomic DNAs were screened using 37 pairs of primers covering promoter region and 26 exons of FVIII gene. Single-strand conformation analysis (SSCA) technique was performed for polymorphism/mutation analyses. We observed polymorph patterns in exon 6, exon 13, exon 14F, exon 19, and exon 25 regions. However, we found no evidence of an association between observed single nucleotide polymorphisms and high thrombosis levels. In conclusion, observed exons polymorphisms do not seem to be associated with a venous thromboembolism.
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    Öğe
    Protective effects of olive oil phenolics and gallic acid on hydrogen peroxide-induced apoptosis
    (Springer Heidelberg, 2012) Erol-Dayi, Ozlem; Arda, Nazli; Erdem, Gunhan
    Olive oil contains several phenolic compounds possessing antioxidant activity. The aim of this study was to investigate the protective effects of olive oil phenolic extract (OOPE) and one of its constituents, gallic acid (GA) against H2O2-induced oxidative stress and apoptotic cell death in HeLa cells, a model for human epithelial cells. The cells were pretreated with nontoxic doses of OOPE or GA for 4, 24 and 48 h, and the intracellular reactive oxygen species (ROS) level was determined, before and after oxidative stress induction with H2O2. As an indicator of apoptosis, caspase 9 activity was measured. All pretreatments reduced ROS generation. Four hour incubation with OOPE or GA completely inhibited ROS generation. Increases in caspase 9 activity by OOPE and GA pretreatment under harsh stress conditions were inhibited 92 and 67.8%, respectively. These results suggest that OOPE and GA act as powerful antioxidants against oxidative stress and exert anti-apoptotic effects.