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    Associations Between Blood Levels of NLRP3 Inflammasome Components and Obsessive Compulsive Disorder
    (Turkish Neuropsychiatric Society, 2023) Tetik, Melike; Direk, Neşe; Önder Uzgan, Betül; Aykaç, Cansu; Ekinci, Burcu; Yaraş, Tutku; Kuruoğlu, Aykut; Özel, Fatih; Ermiş, Çağatay; Alkin, Tunç; Oktay, Yavuz
    Introduction: Even though the effect of inflammation on pathogenesis of obsessive compulsive disorder (OCD) is known, information regarding the underlying mechanisms are yet to be revealed. The NLRP3 inflammasome complex is an important component of the innate immune system that initiates and mediates inflammatory response to a variety of stimuli. This study aims to inquire into a possible association between NLRP3 inflammasome complex and OCD. Methods: This case-control study included 103 participants (51 cases with OCD and 52 healthy controls). All participants were evaluated with the Yale Brown Obsessive Compulsive Scale, Hamilton Depression Scale, and Hewitt Multidimensional Perfectionism Scale. RNA and proteins were extracted from peripheral blood mononuclear cells. Expression of NLRP3 inflammasome components were determined using quantitative real-time polymerase chain reaction (PCR) and Western blotting. Levels of Serum IL-1beta and IL-18 cytokine were determined by ELISA. Results: NEK7 and CASP1 mRNA levels were significantly higher in OCD patients, compared to controls. Pro-caspase-1 protein levels were elevated, as well. Regression analysis showed that NEK7 mRNA and pro-caspase-1 protein levels can differentiate OCD and healthy control groups. Conclusion: Our results provide insight into the molecular alterations that could explain the inflammation-OCD association.
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    Öğe
    Relation of ASC and IL-1beta with neurocog-nitive parameters in patients with Obsessive-Compulsive Disorder
    (Klinik Psikiyatri Dergisi, 2022) Tetik, Melike; Uzgan, Betul Onder; Aykac, Cansu; Ekinci, Burcu; Yaras, Tutku; Kuruoglu, Aykut; Akgul, Ozge
    Objective: It is now well accepted that inflammatory processes play a role in the etiopathogenesis of psychiatric disorders. NLRP3 inflammasome, which is formed by the combination of NLRP3, ASC and Caspase-1 proteins, and its activator NEK7, takes part in these processes. In this study, we aimed to investigate the relationship between inflammatory markers (i.e. IL-1 beta, IL-18, NLRP3 inflammasome protein levels) and cognitive functions in patients with obsessive compulsive disorder (OCD). Method: Forty-two patients between the ages of 18-45 who were diagnosed with OCD were included in the study. Structured Clinical Interview for DSM-IV Axis I Disorders (SCID-I), Yale-Brown Obsessive Compulsive Scale (Y-BOCS), and Hamilton Depression Scale (HAMD) were used for clinical evaluations. In order to evaluate cognitive functions, Trail Making Test (TMT), Berg Card Sorting Test and Category Fluency Test were applied. IL-1 beta and IL-18 cytokine levels were determined by the ELISA method; measurement of the protein amounts of NLRP3, Caspase-1, ASC and NEK7 were performed by Western blotting. Results: Serum IL-1beta was negatively correlated with TMT (B-A) (rs=-.36, p= 0.019). Similarly, ASC was negatively correlated with TMT-B and TMT (B-A) (rs=-.38, p= 0.03; rs=-0.36, p = 0.04, respectively). However, no statistically significant relationship was found between other inflammatory parameters (NLRP3, Caspase-1, and IL-18) and neurocognitive tests. Discussion: Our results indicate that some aspects of neurocognitive impairment in patients with OCD might be related to neuroinflammatory processes, with implications for our understanding of the pathogenesis of OCD.