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Öğe Graft formation in some spur and vigorous apple varieties grafted on Ottawa 3 rootstock: A histological investigation(Wfl Publ, 2010) Yildirim, Adnan N.; Polat, Mehmet; Dolgun, Oguz; Askin, M. Atilla; Gokbayrak, Zeliha; San, BekirThis research was designed to observe the stages of graft union in relation to different growth rate between the rootstock Ottawa 3 and three apple varieties, Smoothee, Summerred and Braeburn. Three to five graft samples of each rootstock/scion combinations were taken at 30 days interval until 12 months passed after from grafting. Samples were fixed in ethanol (70%). Transverse sections in 20-30 mu m thickness were cut with a rotary microtome. Five base stages of graft formation were examined on samples: (1) development and positions of necrotic layers, (2) proliferation of callus cells, (3) formation of callus bridge at the graft interface, (4) cambial continuity and (5) formation of vascular tissues. In the sections prepared from subsequent samples of graft combinations were seen as a good union. Necrotic layers were eliminated in the callus, except for the outer cortex. In outer cortex region, necrotic layers were transformed to bark. Callus filled the gaps between interface of the stock and the scion. Cambial continuity occurred successfully and vascular tissues were connected. Thus, no evidences for tissue incompatibility were found in this study. Histological observations showed a compatible graft union between the graft partners which have different growing characteristics in relation to tissue formation.Öğe Graft union formation of spur apple varieties grafted on different rootstocks(Wfl Publ, 2010) Polat, Mehmet; Dolgun, Oguz; Yildirim, Adnan; Askin, M. Atilla; Gokbayrak, ZelihaThe aim of this research was to observe histological developments during the early stages of graft formation between some spur apple varieties and apple rootstocks in order to assess compatibility/incompatibility related to different growing rate of the plants used. As rootstocks, one year-old clonally propagated A2, B118, M111 (vigorous) and M7, MI6, Pj80 (semi-dwarfing) were used. Scions were taken from one-year-old shoots of Scarlet Spur, Red Spur and Redchief spur apple varieties. Rootstocks were planted in the field in winter 2007 and whip-grafted in April 2008. An evident incompatibility was not observed in the combinations. Sufficient callus proliferation was visible in all the combinations. Successful cambial and vascular connection was apparent in the 90-day sections. In the 120(th) and following sections, it was observed that callus usually filled all the gaps between the rootstock and the scion. Xylem and phloem strands successively bridged the union. However, especially Pj80/Redchief and M16/Red Spur should be followed up in subsequent years in the field conditions because of the excessive callus formation between the stock and the scion.