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Öğe A comparative study of the antimicrobial properties and antioxidant enzyme activities of field-grown and in vitro-propagated plants of endemic Digitalis trojana Ivanina(Inst Bioloska Istrazivanja Sinisa Stankovic, 2017) Corduk, Nursen; Demirbas, Sefer; Dogru, Nurcihan HaciogluThe antimicrobial properties and antioxidant enzyme activities of field-grown and in vitro-propagated plants of Digitalis trojana Ivanina (Helen of Troy foxglove), a perennial endemic plant species of Turkey, were compared. The field work was carried out in May and July 2014, and plant samples of D. trojana were collected from Kazdagi (Turkey). Propagation of D. trojana was achieved by culturing leaf explants on MS medium supplemented with 13.3 mu M 6-benzyl-aminopurine (BA) and 0.53 mu M alpha-naphthaleneacetic acid (NAA). The antimicrobial activity, plant lipid peroxidation levels and antioxidant enzyme (superoxide dismutase (SOD), catalase (CAT), peroxidase (POX), ascorbate peroxidase (APX), glutathione reductase (GR)) activities were analyzed in 12- and 17-week-old in vitro-grown Digitalis plants collected in May and July from two different localities at 430 and 1173 m above sea level. Although the in vitro-propagated plants had very low antagonistic activities compared to field-grown plants, they exhibited remarkably similar antibacterial activities against Escherichia coli ATCC 11230, Pseudomonas aeruginosa ATCC 27853 and Bacillus subtilis ATCC 6633. There were no important differences between plants collected from the two localities (430 and 1173 m a. s. l.). Biochemical analysis showed that the antioxidant enzyme (SOD, APX, GR) activities of field-grown plants were higher than in vitro-grown plants. Also, the difference in altitude at which the plants were grown was apparently linked to decreases in antioxidant enzyme activities, except for POX in field-grown plants collected in July.Öğe Assessment of Nuclear Abnormalities in Erythrocytes of Balkan Pond Turtle Mauremys rivulata (Valenciennes, 1833) (Testudines: Geoemydidae) from the Biga Stream, Canakkale, Turkey(Inst Zoology, Bas, 2019) Corduk, Nursen; Dogru, Nurcihan Hacioglu; Gull, Cigdem; Tosunoğlu, MuratReptilian species are potentially efficient bioindicators of pollution and habitat destruction. Turtles may be especially useful for monitoring the chemical contamination of their habitats. The aim of this study was to assess the genotoxic effect of environmental pollutants on Balkan pond turtle Mauremys rivulata (Valenciennes, 1833) from the Biga Streamp Canakkale, Turkey. Healthy mature animals were collected using the hand-capture method from four stations: estuary zone, city centre, buffer zone and industrial zone of the stream. Blood samples of live specimens were obtained in within one day of their capture. The peripheral blood smears for each specimen were prepared, fixed with methanol for 15 min and dyed with Giemsa stain. Micronuclei and other nuclear abnormalities such as blebbed, kidney-shaped, lobed and notched nuclei were identified. Comparing the nuclear abnormalities in the samples, it was determined that their frequency was highest in the samples collected from the station in the city centre (9.16 +/- 0.02). The results indicated that the levels of genotoxic pollutants in different regions of the Biga Stream lead to DNA damage in Mauremys rivulata.Öğe Green Synthesis and Antibiofilm Activity of Silver Nanoparticles by Camellia sinensis L. (White Tea Leaf)(Kahramanmaras Sutcu Imam Univ Rektorlugu, 2024) Karakas, Ilke; Dogru, Nurcihan Hacioglu; Ozdemir, Bahri EmirhanBiosynthesis of nanoparticles refers to the production or synthesis of nanoparticles using organisms, such as bacteria, fungi, plants or their byproducts. This approach offers several advantages over conventional chemical methods, including eco-friendliness, cost-effectiveness, and potential for large-scale production. The silver nanoparticles (AgNPs) synthesized using aqueous Camellia sinensis L. (white tea leaf) extracts as reducing and stabilizing agents were reported and evaluated for antibiofilm activity against test microorganisms (Acinetobacter baumanii 43252 (Gram +), and Candida albicans ATCC 10231) in the study. The synthesized AgNPs were observed and characterized using Uv-Vis spectroscopic analysis, scanning electron and transmission electron microscopy energy-dispersive spectra and Fourier transform infrared spectroscopy. The synthesized AgNP was also screened for antibiofilm activity against test microorganisms. Our results show that the synthesized AgNPs have the potential to be used for antibiofilm materials and different biomedical applications.Öğe Some Physiological and Biochemical Effects of Bacillus thuringiensis LU3 Biopriming in Common Wheat (Triticum aestivum L.) under Salt Stress(Kahramanmaras Sutcu Imam Univ Rektorlugu, 2023) Yildiz, Muge Teker; Acar, Okan; Ozturk, Furkan; Dogru, Nurcihan HaciogluSalt stress is one of the main abiotic stresses limiting sustainable crop production in the world. Biopriming is the technique involving the use of beneficial and environmentally friendly biological agents to improve the physiological functioning of seeds. Plant growth-promoting rhizobacteria (PGPR) are found in the rhizosphere of plants and have the potential to cope with salinity stress. In this study, the effects of Bacillus thuringiensis LU3 (Bt LU3) biopriming application on two common wheat (Triticum aestivum L.) varieties (Sultan-95 and Tosunbey) under salt stress (0, 100 and 200 mM NaCI) on physiological (root and shoot length, biomass, dry weight, specific leaf area (SLA)), and biochemical parameters (pigment content, total protein content, hydrogen peroxide content (H2O2), lipid peroxidation content (TBARS) and antioxidant enzyme activities (peroxidase activity (POX), glutathione reductase activity (GR))) were investigated. As a result, it was determined that salt-sensitive Sultan-95 had better growth with Bt LU3 biopriming compared to salt-tolerant Tosunbey.