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Öğe Does Glp-2 have a protective effect on cerebral ischemia/reperfusion model?(Tubitak Scientific & Technological Research Council Turkey, 2015) Topaloglu, Naci; Memi, Gulsun; Kaner, Tuncay; Deniz, Mustafa; Sahin, Onder; Guven, Mustafa; Cosar, MuratBackground/aim: To investigate the neuroprotective effects of glucagon-like peptide 2 (Glp-2), which increases cerebral blood flow, on the hippocampal complex after cerebral ischemia/reperfusion (I/R) injury in rats. Materials and methods: Animals were randomized into 4 groups: sham, I/R + 0.9% NaCl, I/R + pre-Glp-2, and I/R + post-Glp-2. Cerebral ischemia was performed via the occlusion of the bilateral internal carotid artery for 40 min and continued with a reperfusion process. At the end of 6 h of reperfusion, animals were decapitated in all groups and brain tissues were removed. Malondialdehyde (MDA) and natural intracellular antioxidant glutathione (GSH) levels and myeloperoxidase (MPO) activities were measured in the left hippocampal tissue. The right hippocampal tissues of all group members were taken for histopathologic study. Results: MDA levels and MPO activities increased from Group I to Group II and decreased from Group II to Groups III and IV. On the other hand, GSH levels were not significantly different among the groups. The number of apoptotic hippocampal tissue cells increased from Group I to Group II and decreased from Group II to Groups III and IV. Conclusion: Our preliminary study revealed that Glp-2 treatment may decrease oxidative damage from I/R in cerebral tissue.Öğe Dopamine - a Preventive Agent for Mesenteric Ischemia and Reperfusion Injury in Abdominal Compartment Syndrome(Wroclaw Medical Univ, 2011) Saracoglu, Ayten; Saracoglu, Kemal T.; Deniz, Mustafa; Ercan, Feriha; Yavuz, Yunus; Gogus, YilmazObjectives. Acutely increased intra-abdominal pressure (IAP) may lead to abdominal compartment syndrome (ACS) and multiple organ failure. In a prospective randomized way, the effect of dopamine infusion (3 mu g/kg/min) on mesenteric perfusion, cytokine levels and intestinal histopathological changes were studied in the presence of ACS. Material and Methods. The study involved 28 male Sprague Dawley rats randomly assigned to four groups (n = 7). The external jugular vein was cannulated for infusions. In group 1, before increasing IAP, a 60-minute infusion of dopamine was performed; following this, IAP was raised and the dopamine infusion was continued for another 60 minutes. In group 2 an IAP of 20 mm Hg was maintained for 60 minutes by air insufflation. In group 3, a dopamine infusion was performed simultaneously with an IAP of 20 mm Hg for 60 minutes. Group 4 was the control. Following this phase, midline laparatomy and superior mesenteric artery (SMA) dissection was carried out in all groups and SMA perfusion was measured continuously for 30 minutes with a Doppler probe. Myeloperoxidase (MPO) activity, lipid peroxidation and glutathione (GSH) levels were measured in tissue samples and histopathological scoring was carried out. Results. The results demonstrated that SMA blood flow was increased in Group 1 and Group 3 (100.77 +/- 2.94 and 93.82 +/- 4.91 mm Hg, respectively) but decreased significantly in Group 2 (74.23 +/- 3.01 mm Hg; p < 0.01). Intestinal tissue malondialdehyde (MDA) levels (24.03 +/- 2.75 nmol/g) and MPO activity (260.5 +/- 11 u/g) were elevated in Group 2; histological scores were elevated in all groups (p < 0.05); and GSH levels were reduced in Group 2 (0.58 +/- 0.24 mu mol/g; p < 0.01). Conclusions. The results indicated that high IAP causes oxidative organ damage and that dopamine may lessen reperfusion-induced oxidative damage by reducing splanchnic perfusion and controlling the reperfusion of the intra-abdominal organs (Adv Clin Exp Med 2011, 20, 5, 613-621).Öğe Dopamine - A preventive agent for mesenteric ischemia and reperfusion injury in abdominal compartment syndrome(2011) Saracoglu, Ayten; Saracoglu, Kemal T.; Deniz, Mustafa; Ercan, Feriha; Yavuz, Yunus; Gogus, YilmazObjectives. Acutely increased intra-abdominal pressure (IAP) may lead to abdominal compartment syndrome (ACS) and multiple organ failure. In a prospective randomized way, the effect of dopamine infusion (3 ?g/kg/min) on mesenteric perfusion, cytokine levels and intestinal histopathological changes were studied in the presence of ACS. Material and Methods. The study involved 28 male Sprague Dawley rats randomly assigned to four groups (n = 7). The external jugular vein was cannulated for infusions. In group 1, before increasing IAP, a 60-minute infusion of dopamine was performed; following this, IAP was raised and the dopamine infusion was continued for another 60 minutes. In group 2 an IAP of 20 mm Hg was maintained for 60 minutes by air insufflation. In group 3, a dopamine infusion was performed simultaneously with an IAP of 20 mm Hg for 60 minutes. Group 4 was the control. Following this phase, midline laparatomy and superior mesenteric artery (SMA) dissection was carried out in all groups and SMA perfusion was measured continuously for 30 minutes with a Doppler probe. Myeloperoxidase (MPO) activity, lipid peroxidation and glutathione (GSH) levels were measured in tissue samples and histopathological scoring was carried out. Results. The results demonstrated that SMA blood flow was increased in Group 1 and Group 3 (100.77 ± 2.94 and 93.82 ± 4.91 mm Hg, respectively) but decreased significantly in Group 2 (74.23 ± 3.01 mm Hg; p < 0.01). Intestinal tissue malondialdehyde (MDA) levels (24.03 ± 2.75 nmol/g) and MPO activity (260.5 ± 11 u/g) were elevated in Group 2; histological scores were elevated in all groups (p < 0.05); and GSH levels were reduced in Group 2 (0.58 ± 0.24 ?mol/g; p < 0.01). Conclusions. The results indicated that high IAP causes oxidative organ damage and that dopamine may lessen reperfusion-induced oxidative damage by reducing splanchnic perfusion and controlling the reperfusion of the intra-abdominal organs. © Copyright by Wroclaw Medical University.Öğe Garlic Extract Ameliorates Renal and Cardiopulmonary Injury in the Rats with Chronic Renal Failure(Taylor & Francis Ltd, 2011) Deniz, Mustafa; Sener, Goksel; Ercan, Feriha; Yegen, Berrak C.Chronic renal failure (CRF) is associated with oxidative stress that promotes production of reactive oxygen species and cytokine release. We aimed to investigate the possible protective and antioxidant effects of aqueous garlic extract (AGE) in a rat model of CRF. Male Sprague-Dawley rats were randomly assigned as either CRF group with 5/6 reduction in the renal mass or sham-operated control group. CRF group received either saline or AGE (250 mg/kg/day/1 mL) orally for 3 weeks. At the end of the 3 weeks, rats were decapitated and trunk blood was collected. Creatinine, blood urea nitrogen (BUN) and lactate dehydrogenase (LDH) activity, and TNF-alpha alpha and IL-1 beta beta levels were measured in the serum samples, while malondialdehyde (MDA), glutathione (GSH) levels, and myeloperoxidase (MPO) activity were determined in the kidney, lung, and heart samples. CRF caused significant decreases in tissue GSH, which were accompanied with significant increases in MDA levels and MPO activities, while the circulating levels of the LDH activity, creatinine, BUN, TNF-alpha alpha, and IL-1 beta beta were elevated. AGE treatment alleviated CRF-induced oxidative changes in the injured tissues, while CRF-induced elevations in the blood levels of the pro-inflammatory cytokines and LDH were reduced. In conclusion, CRF-induced oxidative tissue injury occurs via the activation of pro-inflammatory mediators and by neutrophil infiltration into tissues and that the protective effects of garlic on CRF-induced injury can be attributed to its ability to inhibit neutrophil infiltration and pro-inflammatory mediators. These findings suggest that garlic, as a supplementary to diet, may have a potential therapeutic use in delimitating the systemic oxidant effects of CRF on remote organs.Öğe GLUCAGON LIKE PEPTIDE-2 DO NOT ALTER SEVERITY OF CARRAGENEAN INDUCED KNEE ARTHRITIS BUT HASTENS ACCOMPANYING INTESTINAL INFLAMMATION IN RATS(Springer Tokyo, 2009) Kocakaya, Ozan; Deniz, Mustafa; Ozdemir, Zarife N.; Memi, Gulsun; Bas, Emine; Celikel, Cigdem A.; Eren, Fatih[Anstract Not Available]Öğe Glucagon-like peptide-2 exhibits protective effect on hepatic ischemia-reperfusion injury in rats(Springer, 2015) Topaloglu, Naci; Kucuk, Adem; Yildirim, Sule; Tekin, Mustafa; Erdem, Havva; Deniz, MustafaGlucagon-like peptide-2 (GLP-2) has potent anti-inflammatory effects and protects against experimental ischemia/reperfusion (I/R) injury in pulmonary, intestinal, and myocardial tissue. However, its protective abilities against I/R injury in the liver are unknown. We investigated the potential role of GLP-2 pretreatment on hepatic I/R injury in rats. A total of 24 rats were randomly divided into three groups (n = 8). The first group was the control group; the second group was the vehicle-treated hepatic ischemia/reperfusion (HIR, vehicle saline-treated) group; and the third group was the GLP-2 pretreated I/R (GLP2-IR) group. Each rat in the third group was intraperitoneally administered 5 mu g GLP-2 for 5 d before the procedure. A portal triad was created to induce ischemia with a vascular atraumatic clamp. After 40 min, the clamp was released to initiate hepatic reperfusion for 6 h. Blood samples and tissue specimens from the liver were obtained. Alanine aminotransferase, aspartate aminotransferase, and total bilirubin levels significantly increased in the saline-treated HIR group (P < 0.001), whereas GLP-2 pretreatment significantly decreased their levels (P < 0.01). Our data suggested that GLP-2 pretreatment may have a protective effect on liver I/R injury. However, dose-response studies are necessary to determine the most effective dose.Öğe Prophylactic feeding with immune-enhanced diet ameliorates chemoradiation-induced gastrointestinal injury in rats(Taylor & Francis Ltd, 2010) Atasoy, Beste M.; Deniz, Mustafa; Dane, Faysal; Ozen, Zeynep; Turan, Pinar; Ercan, Feriha; Cerikcioglu, NilguenMaterials and methods: Forty-eight Sprague-Dawley rats were divided into control (C, n = 6), irradiation (IR, n = 14), fluoropyrimidine (5-FU, n = 14)-treated, IR + 5-FU (n = 14)-treated groups. Half of each irradiated and/or 5-FU-treated groups were previously fed with IED containing arginine, omega-3-fatty acids and RNA fragments, while the other half were fed a standard rat diet (SD) for eight days before the induction of IR or injection of 5-FU. In IR groups, whole abdominal irradiation (11 Gy) was performed with 6 MV photons. In the 5-FU groups, fluoropyrimidine (100 mg/kg) was administered intraperitoneally 30 min prior to irradiation. All animals were sacrificed on the 4th day of IR or 5-FU injection. Results: Bacterial colony counts in the ceca and mesenteric lymph nodes of IED-fed rats, which have received either 5-FU and/or irradiation were significantly lower than the corresponding SD-fed groups. Morphometric results revealed that gastric, ileal and colonic injuries were less in IED-treated IR or IR + 5-FU + IED groups, as compared to SD-fed groups. However, IED did not alter DNA fragmentation ratios. Conclusion: Prophylactic feeding of IED has a protective effect on chemoradiation-induced gastrointestinal injury, which appears to involve the eradication of bacterial overgrowth.Öğe Radiation-induced oxidative injury of the ileum and colon is alleviated by glucagon-like peptide-1 and -2(Elsevier Science Bv, 2015) Deniz, Mustafa; Atasoy, Beste M.; Dane, Faysal; Can, Guray; Erzik, Can; Cetinel, Sule; Yegen, Berrak C.Purpose: The present study was conducted to characterize the possible therapeutic effects of glucagon-like peptide (GLP)-1 and GLP-2 against oxidative damage in the ileum and colon of irradiated rats. Methods and materials: Sprague-Dawley rats of both sexes received either a single dose of GLP-1 (0.1 nmol/kg, intraperitoneally, ip; n = 6) 10 min before abdominal irradiation (IR) or two consecutive doses of GLP-2 (7 nmol/kg, ip; n = 6) at 30 and 10 min before IR, while another group was administered vehicle (n = 6) 10 min before IR. Control rats (n = 6) received vehicle treatment without IR. On the fourth day of IR, samples from ileum and colon were removed for histological analysis, for the determination of myeloperoxidase (MPO) activity, malondialdehyde (MDA) and glutathione (GSH) levels, as well as DNA fragmentation ratio, an index of apoptosis. Results: IR-induced oxidative injury in the colonic tissue of vehicle-treated rats, evidenced by elevated MDA levels and MPO activity, as well as depleted colonic GSH levels, was reversed by GLP-2, while GLP-1 reduced IR-induced elevations in colonic MDA levels. IR-induced injury with elevated ileal MDA levels was reduced by GLP-1, while replenishment in GSH was observed in GLP-2-treated rats. Conclusion: Current findings suggest that GLP-1 and GLP-2 appear to have protective roles in the irradiation-induced oxidative damage of the gut by inhibiting neutrophil infiltration and subsequent activation of inflammatory mediators that induce lipid peroxidation. Copyright (C) 2015, The Egyptian Society of Radiation Sciences and Applications. Production and hosting by Elsevier B.V. This is an open access article under the CC BY-NC-ND license.Öğe Saccharomyces boulardii ameliorates clarithromycin- and methotrexate-induced intestinal and hepatic injury in rats(Cambridge Univ Press, 2013) Duman, Deniz Guney; Kumral, Zarife Nigar Ozdemir; Ercan, Feriha; Deniz, Mustafa; Can, Guray; Yegen, Berrak CaglayanSaccharomyces boulardii is a probiotic used for the prevention of antibiotic-associated diarrhoea. We aimed to investigate whether S. boulardii could alter the effects of clarithromycin (CLA) and methotrexate (MTX) on oro-caecal intestinal transit and oxidative damage in rats. Rats were divided into two groups receiving a single dose of MTX (20 mg/kg) or CLA (20 mg/kg per d) for 1 week. Groups were treated with either saline or S. boulardii (500 mg/kg) twice per d throughout the experiment. The control group was administered only saline. Following decapitation, intestinal transit and inflammation markers of glutathione (GSH), malondialdehyde and myeloperoxidase were measured in intestinal and hepatic tissues. CLA and MTX increased intestinal transit, while S. boulardii treatment slowed down CLA-facilitated transit back to control level. Both MTX and CLA increased lipid peroxidation while depleting the antioxidant GSH content in the hepatic and ileal tissues. Conversely, lipid peroxidation was depressed and GSH levels were increased in the ileal and hepatic tissues of S. boulardii-treated rats. Increased ileal neutrophil infiltration due to MTX and CLA treatments was also reduced by S. boulardii treatment. Histological analysis supported that S. boulardii protected intestinal tissues against the inflammatory effects of both agents. These findings suggest that S. boulardii ameliorates intestinal injury and the accompanying hepatic inflammation by supporting the antioxidant state of the tissues and by inhibiting the recruitment of neutrophils. Moreover, a preventive effect on MTX-induced toxicity is a novel finding of S. boulardii, proposing it as an adjunct to chemotherapy regimens.Öğe The Effects of Kefir on the Renal Functions of Rats with Streptozotosin Induced Experimental Diabetes Mellitus(Wiley-Blackwell, 2015) Kahraman, Mustafa; Ertekin, Yusuf Haydar; Turkon, Hakan; Cetinel, Sule; Arik, Muhammet Kasim; Deniz, Mustafa[Anstract Not Available]Öğe The preventive effect of dopamine infusion in rats with abdominal compartment syndrome(2013) Saracoglu, Kemal Tolga; Saracoglu, Ayten; Umuroglu, Tumay; Ugurlu, Mustafa Umit; Deniz, Mustafa; Gogus, Fevzi YilmazBackground: The most significant perfusion disorder of the intra-abdominal viscera occurs in the abdominal compartment syndrome (ACS). Free oxygen radicals diffuse into the body during the reperfusion phase of ACS. Our aim was to determine the effects of dopamine infusion (3 ?g/kg/min) on renal perfusion, cytokine levels, free oxygen radicals, and renal histopathological changes in the presence of ACS in a prospective randomized manner. Methods: Twenty-four male Sprague-Dawley rats were randomly divided into four groups (n = 6). Group 1 was used as control. In group 2, air was inflated until the intra-abdominal pressure (IAP) reached 20 mmHg. In group 3, dopamine was infused for 60 min meanwhile IAP was kept at 20 mmHg. In group 4, dopamine was infused for 60 min before IAP rise. After this phase, renal artery (RA) perfusion was measured continuously. Myeloperoxidase activity (MPO), glutathione (GSH), and lipid peroxidation (MDA) levels were measured in tissue samples and histopathological scoring was performed. Results: Dopamine treatment before and during ACS significantly decreased MPO and MDA levels and also increased renal blood flow and GSH levels. However, histopathological damage was improved simultaneously. Conclusion: Dopamine infusion before and during ACS, increases renal perfusion and decreases free oxygen radicals. According to our findings, dopamine infusion may be proposed for the treatment of ACS and perfusion disorders in critically ill patients. Copyright © 2013 Informa Healthcare USA, Inc.
