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    A new organ preservation solution for static cold storage of the liver. Amniotic fluid
    (Acta Cirurgica Brasileira, 2019) Buyuk, Basak; Demirci, Tuba; Adali, Yasemen; Eroglu, Huseyin Avni
    Purpose: To evaluate the effect of amniotic fluid in liver preservation in organ transplantation, and compare it with standard preservation solutions. Methods: The groups consisted of Group 1: Ringer Lactate (RL) group, Group 2: HTK group, Group 3: UW group, Group 4: AF group. The livers of rats from Group 1, 2, 3, and 4 were perfused and placed into falcon tubes containing RL, HTK, UW, and AF solutions at +4(sic)degrees C, respectively. The tubes were stored for 12 hours in the refrigerator at +4 degrees C. Tissue samples were taken at the 6th and 12th hours for histopathological examinations of the perfused livers, and storage solutions for biochemical analyzes at 6th and 12th hours. Results: AF was shown to maintain organ viability by reducing the number of cells undergoing apoptosis. Histopathological changes such as sinusoidal dilatation, hydropic degeneration, and focal necrosis were found to be similar to the groups in which the standard organ preservation solutions were used. Additionally, the results of INOS, IL-10, and TNF-alpha, which were evaluated immunohistochemically, have been shown to be similar to the UW and HTK groups. Conclusions: AF provided conservation similar to UW and HTK in the 12-hour liver SCS process. The fact that apoptosis values are comparable to standard preservation solutions supports the success of AF in the cold storage of the liver.
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    CAN AMNIOTIC FLUID BE AN ALTERNATIVE ORGAN PRESERVATION SOLUTION FOR COLD RENAL STORAGE?
    (Asoc Regional Dialisis Trasplantes Renales, 2020) Buyuk, Basak; Demirci, Tuba; Adali, Yasemen; Eroglu, Huseyin Avni
    Introduction: Kidney-transplantation is a life-saving treatment option for patients with chronic renal failure. Preserving the viability of the organ from the removal of the organ until transplantation into the recipient is one of the most essential factors affecting postransplant success. Kidney tissue is exposed to ischemia following removal of the organ from the donor, initiating some cellular events. Amniotic fluid (AF) was previously reported as a preservation solution for the liver, but not for the kidney yet. The aim of this study is to investigate the effectiveness of AF as a preserving solution for rat kidneys compared with the University of Wisconsin (UW) and Histidine-Tryptophan-Ketoglutarate (HTK), which are reported to be the most commonly used and preferred preserving solutions. Methods: Forty male Wistar albino rats were used in this study in four experimental groups. Group 1: Ringer Lactate (RL, Control) group, Group 2: HTK group, Group 3: UW group, and Group 4: AF group. A midline incision was performed, and the renal artery was isolated under ketamine and xylazine anesthesia. Solutions relevant for groups (cooled to + 4 degrees C) were used for kidney perfusion. Nephrectomy was applied, and the removed kidneys were placed into + 4 degrees C standard organ storage solution and stored at + 4 degrees C for 12 hours. After 12 hours of storage, samples from the kidney tissues were fixed in 10% neutral buffered formalin. Histopathological, immunohistochemistry evaluation and apoptosis detection via TUNEL method were performed. Results: The results of the AF group were close to those of the UW and HTK groups. Tubular necrosis and vacuolization were high in the RL solution group when compared to the other experimental groups. Immunohistochemistry staining for all three markers (TNF-alpha, IL-18, and iNOS) was decreased in the amniotic fluid group, similar to the UW and HTK groups. Also, the number of apoptotic cells was decreased in the AF group compared to control. Conclusions: UW, HTK, and AF had similar and higher protective effects compared to the RL solution. Thus, AF may be used as an inexpensive and readily available alternative natural tissue preservation solution.
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    Öğe
    Can amniotic fluid be an alternative organ preservation solution for cold renal storage?
    (Asociacion Regional de Dialisi y Transplantes Renales de Capital Federal y Provincia de Buenos Aires, 2020) Büyük, Başak; Demirci, Tuba; Adalı, Yasemen; Eroğlu, Hüseyin Avni
    Introduction: Kidney-transplantation is a lifesaving treatment option for patients with chronic renal failure. Preserving the viability of the organ from the removal of the organ until transplantation into the recipient is one of the most essential factors affecting postransplant success. Kidney tissue is exposed to ischemia following removal of the organ from the donor, initiating some cellular events. Amniotic fluid (AF) was previously reported as a preservation solution for the liver, but not for the kidney yet. The aim of this study is to investigate the effectiveness of AF as a preserving solution for rat kidneys compared with the University of Wisconsin (UW) and Histidine-Tryptophan- Ketoglutarate (HTK), which are reported to be the most commonly used and preferred preserving solutions. Methods: Forty male Wistar albino rats were used in this study in four experimental groups. Group 1: Ringer Lactate (RL, Control) group, Group 2: HTK group, Group 3: UW group, and Group 4: AF group. A midline incision was performed, and the renal artery was isolated under ketamine and xylazine anesthesia. Solutions relevant for groups (cooled to + 4°C) were used for kidney perfusion. Nephrectomy was applied, and the removed kidneys were placed into + 4°C standard organ storage solution and stored at + 4° C for 12 hours. After 12 hours of storage, samples from the kidney tissues were fixed in 10% neutral buffered formalin. Histopathological, immunohistochemistry evaluation and apoptosis detection via TUNEL method were performed. Results: The results of the AF group were close to those of the UW and HTK groups. Tubular necrosis and vacuolization were high in the RL solution group when compared to the other experimental groups. Immunohistochemistry staining for all three markers (TNF-alpha, IL-18, and iNOS) was decreased in the amniotic fluid group, similar to the UW and HTK groups. Also, the number of apoptotic cells was decreased in the AF group compared to control. Conclusions: UW, HTK, and AF had similar and higher protective effects compared to the RL solution. Thus, AF may be used as an inexpensive and readily available alternative natural tissue preservation solution. © 2020, Asociacion Regional de Dialisi y Transplantes Renales de Capital Federal y Provincia de Buenos Aires. All rights reserved.
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    CAN GANODERMA LUCIDUM BE AN ALTERNATIVE NUTRITIONAL SUPPLEMENT FOR ENHANCING SPERM MOTILITY RATE?
    (İzmir Demokrasi Üniversitesi, 2021) Büyük, Başak; Demirci, Tuba; Demir, Neslihan; Türkön, Hakan
    Ganoderma lucidum (GL) is a widely used medicinal mushroom. The therapeutic effect of this fungus on many diseases has been proven by studies. The aim of this study is to assess the effects of low, moderate and high dose GL extract administration on the testis tissue, spermatogenic series cells and sperm motility in rats. 40 Wistar albino rats were randomly divided into 4 groups. Rats in group 1, 2, 3 and 4 were administered 2 ml physiologic serum, 500 mg/kg, 2500 mg/kg, 5000 mg/kg Ganoderma lucidum extract 1 time per day via gavage for 9 days, respectively. For evaluation of sperm motility and histopathological changes, epididymal sperm collection and testis harvesting were done. Blood samples were collected for biochemical analysis. When the Group 1 (control group) is compared with Groups 2, 3 and 4, the Johnsen score and sperm motility in these groups increased and this increase was statistically significant. In conclusion, low, moderate, and high doses of GL extract administered to rats were revealed to increase spermatogenesis, epididymal total sperm count and progressive motile sperm counts. However, it is detected that high doses cause minimal damage to the testis and as the increase in sperm parameters wasn’t significant, it’s concluded that doses for oral use above 2500 mg/kg should be avoided