Yazar "Demirbakan, Burçak" seçeneğine göre listele

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    11-(Triethoxysilyl) Undecanal Agent-Based Biosensor System Using Disposable ITO- PET Electrode for Tumour Necrosis Factor-Alpha Detection
    (Hacettepe University, 2023) Demirbakan, Burçak
    In this study, a label-free electrochemical biosensor system based on a disposable indium tin oxide polyethylene terephthalate (ITO-PET) electrode modified with the 11-(triethoxysilyl) undecanal (11-TESU) agent was developed for the detection of tumour necrosis factor-alpha (TNF-α) in serum. The developed biosensor was observed with electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV) techniques, square wave voltammetry (SWV) and single frequency impedance (SFI) technique which is utilized for the specific interaction between anti-TNF-α and TNF-α antigen. In addition, scanning electron microscopy was used to look at how the morphology of each ITO-PET surface changed (SEM). All parameters such as 11-TESU concentration, anti-TNF-α concentration and anti-TNF-α incubation time, were optimized. The biosensor system was characterized by measuring its linear determination range, repeatability, reproducibility, reusability, storage stability, and surface coverage. The TNF-αelectrochemical biosensor showed high levels of repeatability and reproducibility as well as a large dynamic range of detection (from 0.03 pg mL-1 to 3 pg mL-1). The LOD and LOQ for the biosensor were extremely low at 1x10-4 pg mL-1 and 5x10-4 pg mL-1, respectively. It was applied to real samples to determine whether the proposed biosensor would be useful in clinical settings.
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    A highly sensitive creatine kinase detection in human serum using 11-mercaptoundecanoic acid modified ITO-PET electrodes
    (Academic Press Inc., 2025) Demirbakan, Burçak
    The enzyme creatine kinase (CK) is a biomarker that plays an extremely significant role in the early detection of cardiovascular disorders. Serum levels of CK are regularly monitored in patients with heart attacks, one of the most critical cardiovascular illnesses. In this study, a highly sensitive electrochemical immunosensor system was designed for the importance of early diagnosis of CK. This immunosensor system was developed by immobilizing 11- mercaptoundecanoic acid (11-MuA) on disposable indium tin oxide–polyethylene terephthalate (ITO-PET) electrodes. Electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV) and single frequency impedance (SFI) techniques were utilized throughout the immobilization process during the construction of the immunosensor. In addition, the proposed CK immunosensor system involves thorough analytical research, which may include linear determination range, repeatability, reproducibility, square wave voltammetry, storage capability, and regeneration. The suggested immunosensor was also characterized using scanning electron microscopy (SEM). The proposed immunosensor system demonstrated a broad dynamic range (0.1 pg/mL – 100 pg/mL), as well as a low limit of detection (LOD) and a low limit of quantification (LOQ) of 0.018 pg/mL and 0.0394 pg/mL, respectively. Finally, the immunosensor was tested on human serum samples, proving that it could be utilized in clinical situations.
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    A single-use electrochemical biosensor system for ultrasensitive detection of Aflatoxin B1 in rice, corn, milk, peanut, chili pepper samples
    (Academic Press Inc Elsevier Science, 2024) Demirbakan, Burçak; Tarımeri Köseer, Nur; Uzman, Ecem; Özay, Özgür; Özay, Hava; Sezgintürk, Mustafa Kemal
    Aflatoxin B1, a common food contaminant in peanuts and corn and a genotoxic carcinogen in humans poses a significant risk for hepatocellular carcinoma, making its detection crucial; this study aims to develop a label-free electrochemical biosensor using a disposable indium tin oxide polyethylene terephthalate (ITO-PET) electrode modified with 3-Aminopropyltriethoxysilane for detecting Aflatoxin B1 in real food samples. Initially, optimization steps for the proposed biosensor were conducted using electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) techniques. Characterization steps such as storage capacity, regeneration, and single frequency impedance (SFI) were completed for the proposed disposable biosensor after the optimization steps. The electrochemical biosensor, based on AFB1, exhibited excellent repeatability and reproducibility. It had a broad dynamic detection range from 0.1 fg/mL to 500 fg/mL, with low limits of detection (LOD) and quantitation (LOQ) at 0.19 fg/mL and 0.65 fg/mL, respectively. Finally, the proposed AFB1-based biosensor system was applied to real food samples (rice, chili pepper, milk, corn, and peanuts) for testing and validation.
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    An unusual impedimetric biosensor design based on 3-MPDS for highly sensitive detection of AFB1 in food samples
    (Elsevier, 2024) Demirbakan, Burçak; Tarımeri Köseer, Nur; Özay, Özgür; Özay, Hava; Sezgintürk, Mustafa Kemal
    Aflatoxin B1 (AFB1), a mycotoxin produced by fungi of the genus Aspergillus, particularly Aspergillus flavus, is recognized as the aflatoxin with the highest carcinogenic and mutagenic potential. This study presents a costeffective, disposable AFB1 biosensor system based on 3-mercaptopropyltrimethoxysilane (3-MPDS) and the highly sensitive impedance technique. The surfaces of the ITO-PET (indium tin oxide/polyethylene terephthalate) electrodes were modified with 3-MPDS, and N-hydroxysuccinimide (NHS) was used as a crosslinker. Electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) techniques were employed for immobilization, optimization, and analytical studies. Additionally, the surface morphology of the biosensor was analyzed using scanning electron microscopy (SEM) and atomic force microscopy (AFM). The biosensor demonstrated a linear range from 0.01 fg/mL to 200 fg/mL, with a limit of detection (LOD) of 0.01 fg/mL and a limit of quantification (LOQ) of 0.04 fg/mL. Finally, the proposed biosensor was tested and validated with real food samples, including rice, peanuts, milk, chili pepper, and corn.
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    Conclusion and future prospects
    (Elsevier, 2025) Uludağ Anıl, İnci; Özcan, Burcu; Özyurt, Canan; Demirbakan, Burçak; Kemal Sezgintürk, Mustafa Kemal
    Lateral flow assays (LFAs) are regarded as a significant technique because of their extensive variety of applications. As discussed in this chapter, they are used in a variety of areas, including clinical diagnostics, environmental surveillance, food safety, hormone balance assessment, cancer biomarker identification, and toxin analysis. Although these tests are renowned for their ability to deliver quick and uncomplicated outcomes, they do have several drawbacks, including limited sensitivity and the need for multiple testing. Advancements in technology have provided substantial breakthroughs to address the constraints of LFAs. Recent advancements in nanoparticles, particularly gold nanoparticles, quantum dots, and magnetic nanoparticles, have greatly enhanced the ability to amplify signals and identify many targets. These nanoparticles enhance the sensitivity of LFAs, facilitating the identification of targets even at low concentrations. Furthermore, LFAs that utilize aptamers have the added benefits of increased stability and reduced costs as compared to systems that rely on antibodies. Aptamers can be readily customized with diverse functional groups, offering versatility in experimental design and optimization. Furthermore, the incorporation of surface-enhanced Raman spectroscopy technology enhances the sensitivity of LFAs, allowing for the detection of substances even at lower concentrations. Consequently, the advancement of technology has enhanced the sensitivity and enhanced the user-friendliness of these tests. © 2026 Elsevier Inc. All rights reserved..
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    Fabrication of ultra-sensitive and disposable electrochemical biosensor: Detection of kidney injury molecule-1 protein in urine for diagnosis of kidney injury
    (Elsevier B.V., 2024) Ankara, Elif Ceren; Aras, Sude; Demirbakan, Burçak; Sezgintürk, Mustafa Kemal
    This research study indicates the development of the indium tin oxide-polyethylene terephthalate (ITO-PET) coated electrode-based electrochemical biosensor system to sensitively and specifically detect kidney injury molecule-1 (KIM-1), an acute kidney injury (AKI) biomarker. The ITO-PET electrode surface was modified with 3-(Trimethoxysilyl)-1-propanethiol (3-TMESP) silane agent. Electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV), and single frequency impedance (SFI) techniques were utilized to examine the interactions between anti-KIM-1 antibody and KIM-1 antigen. The ITO-PET electrode surface was observed by scanning electron microscopy (SEM) and atomic force microscopy (AFM) to analyze the morphological characterization at each electrode development stage. Optimization studies have been conducted to determine the optimal concentrations for detecting the 3-TMESP silane agent, the NHS crosslinker, the anti-KIM-1 antibody, and the optimum incubation period for the anti-KIM-1 antibody and the KIM-1 antigen. Analytical characteristic properties of the developed biosensor, including linear determination range, and the studies of reproducibility, regeneration, repeatability, storage life, and selectivity were investigated. The KIM-1 biosensor system, based on 3-TMESP, has a broad linear range and is capable of providing sensitive measurements between 0.1 fg/mL and 1000 fg/mL. The values of low limits of detection (0.26 fg/mL) and of quantification (0.87 fg/mL) were calculated, highlighting its high sensitivity and precision in detecting KIM-1. In addition, five different commercially purchased human urine samples were tested to validate the practicability of the developed biosensor.
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    Lateral flow assays for food analyses: Food contaminants, allergens, toxins, and beyond
    (Elsevier Sci Ltd, 2023) İnce, Bahar; Uludağ, İnci; Demirbakan, Burçak; Özyurt, Canan; Özcan, Burcu; Sezgintürk, Mustafa Kemal
    Food safety is a major issue because of the prevalence of pathogens, chemicals, and other potentially harmful substances in food products. Because of this, the creation of a reliable and accessible tool for checking on food quality is essential. In comparison to traditional techniques, lateral flow tests for the identification of food-borne pathogens are more modern, straightforward, and quick. This review examines the use of lateral flow biosensors for the detection of various food contaminants and the labels used to improve the effectiveness of the system. Since LFAs may be carried out by non-specialists, they are well-suited for on-site testing in a variety of food production and distribution environments.
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    Lateral flow assays using aptamers
    (Elsevier, 2025) Özyurt, Canan; Demirbakan, Burçak; Özcan, Burcu; Uludağ Anıl, İnci; Kemal Sezgintürk, Mustafa Kemal; Ozkan, Sibel A.
    Aptamers, which are short single-stranded nucleic acids or peptides, have become versatile recognition elements as a result of their high specificity, stability, and cost-effectiveness. This chapter offers a thorough examination of aptamer-based lateral flow assays (LFA), with a particular emphasis on their principles, design strategies, and implications for environmental monitoring, food safety, and medical diagnostics. Aptamer-based systems provide superior chemical stability, an extended shelf life, and the ability to undergo a variety of modifications, in contrast to traditional antibody-based LFAs. This chapter also emphasizes the most recent developments in hybrid approaches that integrate aptamers with other biorecognition elements, signal amplification techniques, and aptamer selection methods. The novel concepts described in this chapter have the potential to facilitate the development of diagnostic instruments that are more cost-effective, portable, and efficient, and that have a broad range of applications in biosensor technology. © 2026 Elsevier Inc. All rights reserved..