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Öğe Development of melatonin-embedded PLGA-PEG6000 nanofiber biomaterial, and investigation of the effects on abdominal adhesion formation(SAGE Publications Ltd, 2024) Gökçe, Oruç Numan; Aykora, Damla; Danışman, Merve; Demir, Ufuk; Aydeğer, Cemre; Süner, Salih Can; Oral, Ayhan; Karaboğa, İhsan; Uzun, MetehanAbdominal adhesions are still among the most common postsurgical peritoneal inflammation-related complications. Adhesion-related disorders are still highly costly and prevalent due to advances in surgical techniques, treatment methods, and various drugs. The present study aimed to investigate the effects of Poly (D,L-lactide-co-glycolide) (PLGA)- polyethylene glycol (PEG6000) Nanofiber + Melatonin on the abdominal adhesion model in rats. For this purpose, PLGA-PEG6000 Nanofiber + Melatonin matrix was fabricated and implanted in an experimental abdominal adhesion model in rats. Our study consisted of an in vitro and an in vivo part. The degradation and release profile of the matrix and Melatonin (Mel) embedded matrix was performed in vitro. In vivo, the procedure was carried out with 18 Wistar male rats. Rats were divided into three groups as follows: Sham, Matrix, and Mel + Matrix, respectively. Consequent to degradation and release profiling in vitro, an experimental adhesion model was created and fabricated pure matrix (2 × 2 cm2), and matrix (2 × 2 cm2, 0.25 mg melatonin/per matrix embedded) was applied to injury area in related groups. Intra-abdominal adhesion scores were determined on post-op 21st day, under general anesthesia. Following, cecum, peritoneal tissue, and adhesive bands were harvested. Macroscopic analysis (severity of adhesion formation), Hematoxlyn&Eosin and Masson’s Trichrome staining (for the examination of the levels of infiltration of inflammatory cells, fibrosis, and neovascularization) were performed for the evaluation of the effects of Mel embedded and pure matrix Our results indicated that PLGA-PEG6000 Nanofiber + Melatonin matrix was degraded completely in rats abdominal cavity and significantly reduced adhesion formation compared to other groups macroscopically (p < 0.05). On the other hand, the histopathological analysis indicated that the fabricated matrix reduced inflammatory cell infiltration, fibrosis, and neovascularization levels.Öğe Effect of Quercetin on Colonic Anastomosis Wound Healing(Wiley-Blackwell, 2015) Demir, Ufuk; Edremitlioglu, Mustafa; Kandas, Emel; Sehitoglu, Muserref Hilal[Anstract Not Available]Öğe Effects of Melatonin on Blood Pressure, Oxidative Stress and Placental Expressions of TNF?, IL-6, VEGF and sFlt-1 in RUPP Rat Model of Preeclampsia(Elsevier Science Inc, 2017) Uzun, Metehan; Gencer, Meryem; Turkon, Hakan; Oztopuz, Rahime Ozlem; Demir, Ufuk; Ovali, Mehmet Akif[Anstract Not Available]Öğe Glucagon-like peptide-2 May Assist to Protect against Valproic Acid Induced Hepatic Injury in Rats(2020) Öztopuz, Özlem; Aylanç, Hakan; Şehitoğlu, M.Hilal; Türkön, Hakan; Battal, Fatih; Demir, UfukVPA is widely used in epilepsy and other psychological disorders, increasing the probability of developing non-alcoholic liver diseasein long-term treatments. GLP-2 is a proglucagon belonging to the peptide family expressed in the intestine, pancreas and brain to date.Although there are many studies on the use of GLP-2 for therapeutic purposes on the gastrointestinal system, its effect on liver toxicityis unknown. We aimed to investigate the effect of GLP-2 administration on hepatic function in a rat model with VPA-inducedhepatotoxicity. Rats were injected intraperitoneally at 500 mg/kg and GLP-2 5µg/kg a day. The rats (200-250g) were separated into fourgroups (n=7). Group C was administrated 1 mL of 0.9% SF, Group GLP treated with GLP-2 (5µg/kg/day), Group GLP+VPA werereceived GLP-2 (5µg/kg) 1 h prior to VPA (500 mg/kg), Group VPA received VPA (500 mg/kg), 1 h prior to 1 mL of 0.9% SF ip (n=7).Liver tissues were used to investigate effects of VPA and GLP-2 in the liver 15 days after application. While VPA caused moderate butsignificant liver damage according to biochemical results, mRNA expression of cytokines were found to significantly increase after theday 15. VPA administration significantly induced expression of Interleukin 1 beta (IL-1?), Tumor necrosis factor alpha (TNF-?),Interleukin 10 (IL-10). In contrast, GLP-2 treatment reduced expression of IL-1?, TNF-? and IL-10. Also malondialdehyde (MDA),glutathione s-transferase (?-GST), superoxide dismutase activities (SOD), total antioxidant status (TAS) and total oxidant status (TOS)levels were estimated. GLP-2 had positive effects on both liver enzymes and oxidative stress markers in VPA-induced hepatotoxicity.These results suggest that endogenous GLP-2 administration is associated with a mechanism that moderately protects liver tissue.Öğe Healing Effects of L-Carnitine on Colon Anastomosis Wound on Experimental Colon Anastomosis(Wiley-Blackwell, 2015) Kandas, Emel; Edremitlioglu, Mustafa; Demir, Ufuk; Erbil, Guven; Sehitoglu, Muserref Hilal[Anstract Not Available]Öğe Healing effects of L-carnitine on experimental colon anastomosis wound(Marmara University, 2023) Kandaş, Emel; Edremitlioğlu, Mustafa; Demir, Ufuk; Erbil, Güven; Şehitoğlu, Muşerref HilalObjective: The purpose of this study is to examine the effects of L-carnitine on healing of experimental colon anastomosis injury in early and late period.Materials and Methods: Forty female Wistar-Albino rats were used in this study. The rats were divided into 4 groups (CONT-3, CONT-7, CARN-3, and CARN-7). Injury healing was evaluated for CONT-3 group on the 3rd day and for CONT-7 group on the 7th day following the anastomosis. Following the operation, CARN-3 and CARN-7 groups were intraperitoneally administered with 100 mg/ kg/day L-carnitine and injury healing was evaluated on the 3rd and 7th days. Injury strength, histological evaluation and antioxidant enzyme activities and oxidant damage were determined in tissue samples of anastomosis area.Results: Bursting pressure levels and histological scoring values of CARN-3 group were found to be higher than the CONT-3 group (p<0.05). Antioxidant enzyme activities were found to be high in groups which were administered with L-carnitine, and oxidant damage was found to be significantly low in CARN-7 group (p<0.05).Conclusion: It was seen that L-carnitine speeds up the injury healing process and increases the injury strength and antioxidant capacity in early period. Increase in antioxidant enzyme activities was observed to be continued in late period as well.Öğe Hyperbaric oxygen treatment ameliorates gentamicin-induced nephrotoxicity and expression of kidney injury molecule 1 in the rat model(Undersea & Hyperbaric Medical Soc Inc, 2019) Oztopuz, Ozlem; Turkon, Hakan; Sehitoglu, Muserref Hilal; Buyuk, Basak; Uzun, Metehan; Ovali, Mehmet Akif; Demir, UfukIn recent years, hyperbaric oxygen (HBO2) therapy has been considered as an effective method for the treatment of gentamicin (GM)-induced renal toxicity. However, the findings related to the use of HBO2 for GM toxicity are limited and contradictory. The aim of this study is to investigate the protective role of HBO2 on GM-induced nephrotoxicity. For this purpose, Wistar albino rats (n=28) were randomly divided into four equal groups: C, HBO2, GM and GM+HBO2. GM (100 mg/kg, ip) and HBO2 were applied over seven days. On the eighth day blood and kidney tissue samples were harvested. The albumin, creatinine, and urea levels were determined from serum samples. Superoxide dismutase (SOD), glutathion peroxidase (GSH-Px) activities, malondialdehyde (MDA), total antioxidant status (TAS) and total oxidant status (TOS) values were analyzed spectrophotometrically. The relative expression level of TNF-alpha, IL-1 beta and Kim-1 gene were determined by qRT-PCR assays; histopathologic investigation was completed in kidney tissue samples. Serum urea, albumin and creatinine levels significantly increased in the GM group compared to the GM+HBO2 group. For antioxidant parameters the GM+HBO2 group was not statistically different from the C group but was significantly different compared with the GM group. TNF-alpha, IL-1 beta and Kim-1 gene expression levels in the GM group were statistically increased compared to the GM+HBO2 group (p=0.015, p=0.024, p=0.004) respectively. Severe tubular necrosis, epithelial desquamation and mild peritubular hemorrhage were observed in the GM-administrated group, while HBO2 exposure ameliorated these alterations. In conclusion, HBO2 exposure may be defined as a potential method for the prevention of GM-induced renal toxicity.Öğe Investigation of Lycium barbarum Effects on Ovarium Damage Exposed to N-Methyl-N-Nitrourea(Springer, 2025) Eroglu, Hueseyin Avni; Buyuk, Basak; Aydeger, Cemre; Demir, Ufuk; Makav, MustafaN-Methyl-N-nitrourea (MNU), is one of the N-nitroso compounds that people are commonly exposed to in various exogenous ways such as diet, tobacco smoke, cosmetics, household goods, indoor air, occupational exposure, etc. It is known that the compound damages organs and tissues in the body, and one of the affected structures is the ovarium. Lycium barbarum, used in medication in ancient China, has become more popular in recent years. Previous studies have mentioned that polysaccharide ingredients are the most bioactive parts of Lycium barbarum. Therefore, this study aims to investigate the effects of Lycium barbarum polysaccharides (LBP) on ovarian tissue damage exposed to MNU. In accordance with this purpose, a total of 12 21-day-old female Wistar Albino rats were divided into two groups: sham and treatment. The animals were administrated with 50 mg/kg MNU (i.p.) on days 21, 28, 35, and 42. The treatment group was treated with 800 mg/kg LBP via intragastric for 30 days. At the end of the study, animals were sacrificed under general anaesthesia, and the ovarian tissues were harvested. Shrinkage of follicles, lytic oocytes, and disintegrated stroma parameters were evaluated histopathologically. The genetic assessment of the Bcl-2, Bax, Bcl-2/Bax ratio, Caspase 3, p53, and p27 expression levels were determined. The histopathological parameters between the groups were statistically significant (for all parameters p < 0.001). Moreover, the Bcl-2, Bcl-2/Bax ratio, and p53 relative fold changes were significantly increased in the treatment group (p < 0.001, p = 0.045, and p = 0.011, respectively). The results revealed that LBP has ameliorative effects on MNU-induced ovarian tissue damage. Further studies are required to clarify the main underlying mechanisms.Öğe Melatonin Improves Left Ventricular Mitochondrial Dynamics in Rats(Pleiades Publishing Inc, 2022) Uzun, Metehan; Oztopuz, Ozlem; Eroglu, Huseyin Avni; Doganlar, Oguzhan; Doganlar, Zeynep Banu; Ovali, Mehmet Akif; Demir, UfukThere is increasing awareness that efficient and regular mitochondrial dynamics improvement cardiac function and affects the quality of life. Melatonin is a main pineal gland hormones and ameliorates mitochondrial dynamics in many cardiac disorders. For that purpose, we administrated melatonin to healthy rats all day long in order to investigate change in left ventricle mitochondrial dynamics both in the end of the nighttime and daytime. Twenty male Wistar rats (3-4 months age) were randomly assigned into Control (C; n = 10) and Melatonin groups (MEL; 10 mg/kg melatonin added drinking water, n = 10). On the 5th day of the study, 5 rats from the groups were randomly selected and euthanized at 08:00 AM and the remaining 5 rats were euthanized at 20:00 PM from each groups and samples of left ventricle (LV) tissue were harvested. Quantitative real-time PCR and western blot analysis demonstrated that melatonin acts preventive role on mitochondrial fusion and mitophagy through the DRP1/FIS1 and BNIP3/NIX axis, respectively. Additionally, melatonin administration significantly reduced P21 activation, induced cell cycle arrest, P27, finally regulated caspase-depended mitochondrial apoptosis signals in a time dependent manner. Our results suggest that melatonin may emerge as a therapeutic candidate to protect the bioenergetic dynamics of mitochondria in hearth.Öğe Melatonin regulates oxidative stress and apoptosis in fetal hearts of pinealectomised RUPP rats(Taylor & Francis Inc, 2020) Doganlar, Oguzhan; Doganlar, Zeynep Banu; Ovali, Mehmet Akif; Guclu, Orkut; Demir, Ufuk; Dogan, Ayten; Uzun, MetehanObjective This study aimed to investigate the effects of melatonin on cardiac oxidative stress and apoptosis in the fetal heart in RUPP rats. Methods The fetal heart samples were obtained from melatonin administrated RUPP rats Results Our results indicate that preeclampsia exacerbated by melatonin deficiency triggers hypoxic conditions, both mis/un-folded protein response, oxidative stress-induced DNA damage and apoptosis. Melatonin treatment provided significant therapeutic effects on fetal hearts via regulating all these stress response at cellular and molecular levels. Conclusion Melatonin may be considered as a potential molecule for development of preventive strategies to reduce the PE induced risk of cardiovascular diseases in offspring.Öğe Quercetin associated with dimethylsulfoxide has a curative effect on experimental colon anastomosis injury(Acta Cirurgica Brasileira, 2020) Demir, Ufuk; Edremitlioglu, Mustafa; Kanda, Emel; Sehitoglu, Muserref Hilal; Kilinc, NihalPurpose: To examine the effects of quercetin on healing of experimental colon anastomosis injury in early and late period. Methods: Eighty male Wistar-Albino rats were divided into 8 groups. For all groups, left colons of the rats were resected and for the rest end-to-end anastomosis was performed. Two of the groups for which the experiment protocol was ended on the 3rd and 7th day following the anastomosis were not administered with either quercetin or dimethylsulfoxide DMSO, whereas two other groups were administered with DMSO only, and four other groups were administered with quercetin dissolved in DMSO in doses of 20 and 100 mg/kg during the protocol. At the end of the study, anastomosis line was resected, histopathological evaluation was performed and bursting pressure, malondialdehyde, superoxide dismutase, catalase, and hydroxyproline levels were measured. Results: Quercetin significantly increased hydroxyproline, superoxide dismutase, catalase levels, histopathological healing score, bursting pressure values and decreased malondialdehyde level in early period. It also significantly increased superoxide dismutase, catalase, and hydroxyproline levels and decreased malondialdehyde level in late period. Conclusion: it was seen that quercetin speeds up the injury healing process and reveals an antioxidant effect, specifically in early period.Öğe Quercetinin kolon anastomoz yarası iyileşmesine etkisi(Çanakkale Onsekiz Mart Üniversitesi, 2017) Demir, Ufuk; Edremitlioğlu, MustafaKolon anastomozlarında cerrahi tekniklerde ilerlemeye rağmen anastomoz kaçakları ve yara iyileşmesinde gecikmeler görülmektedir. Yara iyileşmesinin iyi bir şekilde olmaması anastomoz kaçağı riskini arttırır ve ölümle sonuçlanabilen durumların ortaya çıkmasına neden olabilir. Yara iyileşmesi üzerine etkileri araştırılan moleküllerden biri quercetindir. Quercetin antioksidan özelliğe sahip bir moleküldür ve antioksidan aktivite ile yara iyileşmesine katkıda bulunduğu bildirilmiştir. Amacımız, quercetinin barsak anastomozu sonrasında cerrahi yara iyileşmesini nasıl etkileyeceğini ortaya çıkarmaktır. Çalışmada 230-260 gr ağırlığında 80 adet erkek Wistar Albino cinsi ratlar kullanılmıştır. Her birinde 10'ar hayvan bulunan 8 grup oluşturulmuştur. Gruplar; 1) Kontrol 3 günlük (K3 grubu), 2) Kontrol 7 günlük (K7 grubu), 3) Dimetil sülfoksit kontrol 3 günlük (DMSO3 grubu), 4) Dimetil sülfoksit kontrol 7 günlük (DMSO7 grubu), 5) Quercetin 20 mg/kg 3 günlük (Q3/20 grubu), 6) Quercetin 100 mg/kg 3 günlük (Q3/100 grubu), 7) Quercetin 20 mg/kg 7 günlük (Q7/20 grubu), 8) Quercetin 100 mg/kg 7 günlük (Q7/100 grubu) şeklinde ayrılmıştır. Quercetin dimetil sülfoksit (DMSO) içinde çözdürülerek, 100 mg/kg ve 20 mg/kg şeklinde 2 farklı dozda intraperitoneal olarak 24 saatte bir verilmiştir. Üçüncü ve 7. günlerdeki yara iyileşmesi patlama basıncı saptanarak değerlendirilmiştir. Daha sonra aldığımız anastomoz bölgesi doku örneklerindeki antioksidan enzim aktiviteleri, oksidan hasarın derecesi ve yara yerinin histopatolojik iyileşmesi belirlenmiştir. Sonuç olarak quercetinin anastomoz sonrası yara iyileşmesine katkı sağladığı görülmüştür. Anahtar sözcükler: Quercetin, kolon anastomozu, antioksidan, patlama basıncı, yara iyileşmesi.Öğe The Effects of Melatonin on Blood Pressure, Oxidative Stress and Expression Levels of Placental Tnf?, IL-6, VEGF and Sflt-1 Genes in RUPP Rat Model of Preeclampsia(Wiley, 2017) Uzun, Metehan; Oztopuz, Rahime Ozlem; Turkon, Hakan; Gencer, Meryem; Demir, Ufuk; Ovali, Mehmet Akif[Anstract Not Available]
