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Öğe Antiparasitic and Antibacterial Functionality of Essential Oils: An Alternative Approach for Sustainable Aquaculture(MDPI AG, 2021) Dawood, Mahmoud A. O.; El Basuini, Mohammed E.; Zaineldin, Amr I.; Yılmaz, Sevdan; Hasan, Md. Tawheed; Ahmadifar, Ehsan; El Asely, Amel M.Using synthetic antibiotics/chemicals for infectious bacterial pathogens and parasitic disease control causes beneficial microbial killing, produces multi-drug resistant pathogens, and residual antibiotic impacts in humans are the major threats to aquaculture sustainability. Applications of herbal products to combat microbial and parasitic diseases are considered as alternative approaches for sustainable aquaculture. Essential oils (EOs) are the secondary metabolites of medicinal plants that possess bioactive compounds like terpens, terpenoids, phenylpropenes, and isothiocyanates with synergistic relationship among these compounds. The hydrophobic compounds of EOs can penetrate the bacterial and parasitic cells and cause cell deformities and organelles dysfunctions. Dietary supplementation of EOs also modulate growth, immunity, and infectious disease resistance in aquatic organisms. Published research reports also demonstrated EOs effectiveness against Ichthyophthirius multifiliis, Gyrodactylus sp., Euclinostomum heterostomum, and other parasites both in vivo and in vitro. Moreover, different infectious fish pathogenic bacteria like Aeromonas salmonicida, Vibrio harveyi, and Streptococcus agalactiae destruction was confirmed by plant originated EOs. However, no research was conducted to confirm the mechanism of action or pathway identification of EOs to combat aquatic parasites and disease-causing microbes. This review aims to explore the effectiveness of EOs against fish parasites and pathogenic bacteria as an environment-friendly phytotherapeutic in the aquaculture industry. Moreover, research gaps and future approaches to use EOs for sustainable aquaculture practice are also postulated.Öğe Benefits of Dietary Polyphenols and Polyphenol-Rich Additives to Aquatic Animal Health: An Overview(Taylor and Francis Ltd., 2021) Ahmadifar, Ehsan; Yousefi, Morteza; Karimi, Msoumeh; Fadaei Raieni, Raha; Dadar, Maryam; Yılmaz, Sevdan; Dawood, Mahmoud A. O.; Abdel-Latif, Hany M. R.Polyphenols are plant-derived compounds with known biological activities and potential health benefits. Over the past decade, there are an increasing number of studies have investigated the application of polyphenols and polyphenol-rich additives in aquaculture as functional feed additives. There are several types of polyphenolic compounds such as flavonoids, phenolic acids, lignans, and stilbenes of widely known beneficial influences on the overall performances and immunity of fish, and thereby improving the health status and production of fish farms. This review discusses the beneficial applications, optimum recommended dose, and potential side effects of these natural compounds on the growth performance, proximate body composition, immune responses, disease resistance, reproductive performance, and fillet quality of different fish species. Also, it points out that the biological functions and mechanisms associated with the polyphenolic compounds involved in the pathways of antioxidant and pro-oxidant activities, as well as the modulation of gene expression and different immune parameters. The output of this review article provided overwhelming evidence to support the claim that natural polyphenols can be considered as relatively safe and viable alternatives to synthetic chemical compounds that not only to improve the fish health status but also to enhance the fish quality, productivity, and food safety while reducing the use of chemicals and antibiotics in the aquatic eco-systems.Öğe Exploring the Roles of Dietary Herbal Essential Oils in Aquaculture: A Review(Mdpi, 2022) Dawood, Mahmoud A. O.; El Basuini, Mohammed F.; Yılmaz, Sevdan; Abdel-Latif, Hany M. R.; Alagawany, Mahmoud; Kari, Zulhisyam Abdul; Razab, Mohammad Khairul Azhar AbdulSimple Summary Essential oils, also known as aetheroleum, have a variety of therapeutic properties, including analgesic, adaptogen, and astringents, among others. Essential oils have potential growth-promoting, antibacterial, and immunostimulant effects for several fish species. Furthermore, they potentiate the antioxidative capacity and the resistance of aquatic animals against infectious diseases. This article spotlights on the essential oils derived from selected medicinal plants, and their roles in the improvement of the performances of aquatic animals. The aquaculture sector is one of the main activities contributing to food security for humanity around the globe. However, aquatic animals are susceptible to several farming stressors involved in deteriorated growth performance, reduced productivity, and eventually high mortality rates. In some countries still, antibiotics and chemotherapies are comprehensively applied to control biotic stressors. Aside from the apparent benefits, the continuous usage of antibiotics develops bacterial resistance, deteriorates bacterial populations, and accumulates these compounds in the aquatic environment. Alternatively, environmentally friendly additives were used to avoid the direct and indirect impacts on the aquatic ecosystem and human health. In aquaculture, medicinal herbs and extracts are extensively used and approved for their growth-promoting, anti-inflammatory, and antioxidative properties. Herbal essential oils contain many bioactive components with powerful antibacterial, antioxidative, and immunostimulant potentials, suggesting their application for aquatic animals. Essential oils can be provided via diet and can benefit aquatic animals by improving their well-being and health status. The use of essential oils in aquafeed has been studied in a variety of aquatic animals to determine their beneficial roles and optimum doses. The outputs illustrated that herbal essential oils are exciting alternatives to antibiotics with prominent growth promotion, antioxidative, and immunostimulant roles. Herein, we reviewed the beneficial roles of essential oils in aquaculture. This review also aims to describe trends in herbal essential oils use, mainly in commercial fish species, and to analyze different factors that affect essential oils' efficacy on the growth performance, antioxidative, and immune responses of finfish species.Öğe The impact of acute cold water stress on blood parameters, mortality rate and stress-related genes in Oreochromis niloticus, Oreochromis mossambicus and their hybrids(Elsevier Ltd, 2021) Yılmaz, Sevdan; Ergün, Sebahattin; Çelik, Ekrem Şanver; Banni, Mohamed; Ahmadifar, Ehsan; Dawood, Mahmoud A. O.This study aims to evaluated the response of Oreochromissp. to cold stress. Two experiments were conducted involving a total of 1080 juvenile Oreochromis niloticus, O. mossambicus, O. niloticus♂ × O.mossambicus♀=F1♂ × O.mossambicus ♀ (Hybrid 1; H1) and O. mossambicus♂ × O. niloticus♀ (Hybrid 2; H2). In the 1st experiment, fish were exposed to cold water (12 °C) for 24 h and then hematological parameters, serum biochemical variables, innate immune responses, antioxidant status, and liver gene expression responses (hsp70, hsp27, hsp90, hsp40, cat, sod, eef1a1 and calreticulin) were analyzed. Hematological and serum biochemical responses involved species-specific differences. At optimal temperatures (28 °C), respiratory burts activity (RBA) and myeloperoxidase (MPO) values of H1, H2 and O. mossambicus were significantly higher than O. niloticus (p< 0.05). While the RBAvalue of O. mossambicus decreased after exposure to cold water (p< 0.05), lysozyme activities of O. niloticus and H2 and MPO activities of all experimental fish increased significantly (p< 0.05). At control conditions (28 °C), cortisol levels were found to be higher in O. mossambicus than in H1 and O. niloticus (p< 0.05). A significant increase in cat and sod transcripts was observed in liver of fish being very pronounced in O. mossambicus and H2. The highest up-regulation was observed for hsp70 target where the lowest but significant up-regulation was observed for hsp90 gene. In 2nd experiment, water temperature was gradually decrease from 28 °C to 12 °C (average, 1 °C/1 h).Survival rates of H1 and H2 were found to be different compared to O. mossambicus and O. niloticus (p< 0.05) after 20 days of cold water challenge.O. mossambicus was the most cold-sensitive group, followed by the H2, H1 and O. niloticus. Our data should be carfully considered in view of the possible physiological and anti-stressor responses being species-specific in fish.Öğe Lemon, Citrus aurantifolia, peel and Bacillus licheniformis protected common carp, Cyprinus carpio, from Aeromonas hydrophila infection by improving the humoral and skin mucosal immunity, and antioxidative responses(Wiley Periodicals LLC, 2021) Sadeghi, Faeze; Ahmadifar, Ehsan; Shahriari Moghadam, Mohsen; Ghiyasi, Maryam; Dawood, Mahmoud A. O.; Yılmaz, SevdanThe role of dietary lemon peel (LM) and/or Bacillus licheniformis (BL) on the growth, immunity, and resistance against Aeromonas hydrophila in common carp, Cyprinus carpio was investigated in this study. LM and BL were included in diets at 0% (T0), 108 CFU/g BL (T1), 1.5% LM and 108 CFU/g BL (T2), and 3% LM and 108 CFU/g BL (T3). Fish fed with T1, T2, or T3 had higher weight gain, specific growth rate, white blood cells count, and blood total protein with lower feed conversion ratio than T0 group (p <.05). The albumin increased significantly (p <.05) in fish fed both BL and LM (T3). The serum superoxide dismutase (SOD), catalase, lysozyme, and bactericidal activities were significantly increased in fish fed both BL and LM (T2 and T3), while serum glutathione peroxidase increased in fish fed BL (T2) (p <.05). Fish fed T1, T2, and T3 diets displayed higher SOD and lower malondialdehyde than fish fed T0 (p <.05). After the A. hydrophila challenge, the mortality rate was significantly lower in T1, T2, and T3 groups than the T0 group (p <.05). The obtained results revealed that LM and BL could be used to increase resistance against A. hydrophila infection in carp. However, further field studies should be performed to confirm the obtained results.Öğe Selenium Nanoparticles as a Natural Antioxidant and Metabolic Regulator in Aquaculture: A Review(MDPI, 2021) Dawood, Mahmoud A. O.; El Basuini, Mohammed F.; Yılmaz, Sevdan; Abdel-Latif, Hany M. R.; Kari, Zulhisyam Abdul; Abdul Razab, Mohammad Khairul Azhar; Ahmed, Hamada A.; Alagawany, Mahmoud; Gewaily, Mahmoud S.Balanced aquafeed is the key factor for enhancing the productivity of aquatic animals. In this context, aquatic animals require optimal amounts of lipids, proteins, carbohydrates, vitamins, and minerals. The original plant and animals’ ingredients in the basal diets are insufficient to provide aquafeed with suitable amounts of minerals. Concurrently, elements should be incorporated in aquafeed in optimal doses, which differ based on the basal diets’ species, age, size, and composition. Selenium is one of the essential trace elements involved in various metabolic, biological, and physiological functions. Se acts as a precursor for antioxidative enzyme synthesis leading to high total antioxidative capacity. Further, Se can enhance the immune response and the tolerance of aquatic animals to infectious diseases. Several metabolic mechanisms, such as thyroid hormone production, cytokine formation, fecundity, and DNA synthesis, require sufficient Se addition. The recent progress in the nanotechnology industry is also applied in the production of Se nanoparticles. Indeed, Se nanoparticles are elaborated as more soluble and bioavailable than the organic and non-organic forms. In aquaculture, multiple investigations have elaborated the role of Se nanoparticles on the performances and wellbeing of aquatic animals. In this review, the outputs of recent studies associated with the role of Se nanoparticles on aquatic animals’ performances were simplified and presented for more research and development.Öğe Shrimp vibriosis and possible control measures using probiotics, postbiotics, prebiotics, and synbiotics: A review(Elsevier, 2022) Abdel-Latif, Hany M. R.; Yilmaz, Ebru; Dawood, Mahmoud A. O.; Ringo, Einar; Ahmadifar, Ehsan; Yılmaz, SevdanThe shrimp industry has been greatly expanded in the past two decades in several countries throughout the globe to provide human beings with nutritious seafood and healthy protein. In this concern, shrimp farmers have tried to increase their production rates by intensifying shrimp in culture facilities. However, intensive farming may increase the possibilities of the entrance of emergent pathogens, which will dramatically cause severe economic loss and reduce the profitability of shrimp culture. Shrimp vibriosis is one of the devasting bacterial diseases that commonly affect a wide range of shrimp species. There are many diseases associated with Vibrio infections in shrimp, such as red disease, seagull syndrome, Bright red syndrome, luminous vibriosis, shell disease, zoea II syndrome, septic hepatopancreatic necrosis, early mortality syndrome or acute hepatopancreatic necrosis disease. Owing to the above, preventive strategies are considered critical and even necessary to be applied to prevent the negative impacts of shrimp vibriosis. On the other hand, the FDA has banned antibiotic usage for food animals because of their negative consequences on the host, human beings, and environment. Researchers have paid great interest in finding possible, effective, economical, and environmentally safe alternatives to antibiotics. Currently, vaccination, water quality control, bacteriophages phage therapy, biosecurity protocols, herbal immunomodulators, and several others are extensively utilized to reduce the impacts of shrimp vibriosis. The trend of using probiotics, postbiotics, parabiotics, prebiotics, and synbiotics in controlling shrimp diseases has been comprehensively evaluated in several publications and their efficacy to limit the usage of antibiotics in aquaculture has been proven. The possible efficacy of pro-, post-, pre-, and synbiotics as possible control methods of shrimp vibriosis has been assessed in many reseach studies. Of interest, the present review article draws the most current knowledge on the applications and beneficial roles of pro-, post-, pre-, and synbiotics to fortify shrimp immunity and decrease the mortalities caused by vibriosis in several shrimp species. It will also spotlight the proposed modes of action of pro-, post-, pre-, and synbiotics and discuss the research gaps that merit additional explanations. Overall, the literature will be valuable to enhance the sustainability of shrimp culture, and increase the welfare and resistance of cultured shrimp against vibriosis.Öğe The effectiveness of Arthrospira platensis and microalgae in relieving stressful conditions affecting finfish and shellfish species: An overview(Elsevier, 2022) Abdel-Latif, Hany M. R.; El -Ashram, Saeed; Yılmaz, Sevdan; Naiel, Mohammed A. E.; Kari, Zulhisyam Abdul; Hamid, Noor Khalidah Abdul; Dawood, Mahmoud A. O.Farmed fish and shrimp are continuously challenged by multiple stressors during their life stages, such as hypoxia, pH fluctuations, different salinities, high nitrite, un-ionized ammonia, injury during handling, inade-quate nutrition, or food shortage, which can eventually adversely impact their health, welfare, and growth rates. Besides, these stressors can weaken production and decrease their resistance to diseases. Scientists and re-searchers have been making concerted efforts to find new, safe, and inexpensive supplements to mitigate the negative influences of stressors and thereby enhance the productivity of farmed aquatic animals. Some micro-algae are microscopic unicellular organisms that were found to be promising feed supplements due to their richness in important nutrients such as minerals and vitamins. Moreover, some microalgae contain several bioactive phytochemicals that exhibit anti-inflammatory, antioxidant, and immunomodulatory properties. Several field-controlled studies provided evidence that using microalgae as feed supplements led to improved growth, physiological functions, immunity, antioxidant capacity, and disease resistance in farmed finfish and shellfish species. This review article emphasizes the beneficial role of the cyanobacterium Arthrospira platensis and seven microalgal species, including Chlorella vulgaris, Parietochloris incisa, Dunaliella salina, Aurantiochytrium sp., Haematococcus pluvialis, Tetraselmis sp., and Nannochloropsis oculata in mitigating stress effects in farmed finfish and shellfish species. The conclusions of this article throw light on the potential benefits of using mi-croorganisms in aquaculture.Öğe The Feasibility of Using Yellow Mealworms (Tenebrio molitor): Towards a Sustainable Aquafeed Industry(MDPI AG, 2021) Shafique, Laiba; Abdel-Latif, Hany M. R.; Hassan, Faiz-ul; Alagawany, Mahmoud; Naiel, Mohammed A. E; Dawood, Mahmoud A. O.; Yılmaz, Sevdan; Liu, QingyouThe success of the aquafeed industry mainly depends on the availability of raw ingredients with high nutritional value, such as fishmeal (FM). However, the increased demand for FM elevates its prices and leads to high feed costs. Thus, there is an urgent need to find suitable alter-natives for FM in fish diets to achieve sustainability in aquaculture. Currently, attention is being paid to the possibility of using insect meals as FM substitutes in aquafeed because of their relatively high nutritional quality. TM is one of those insects that can be regarded as a unique candidate because of its relatively high nutritional value. TM are rich sources of essential amino acids (methio-nine), lipids, and fatty acids, which vary based on the developmental stage of the worms. Although TM have an abundant amount of chitin as a fiber source and other anti-nutritional factors, numer-ous studies have investigated the efficacy of partial or complete substitution of FM by T. molitor in fish diets. In this context, we reviewed the current research findings on the achievable inclusion levels of T. molitor versus FM substitution in the diets of several finfish and shellfish species. We discussed the potential use of T. molitor as an FM substitute in fish diets and evaluated its effects on growth, biometric indices, and body composition. Besides, the hematological parameters, immunological responses, antioxidative efficacy, intestinal health status, and sensory criteria of fish fed T. molitor-based diets were also assessed.Öğe The Gene Regulatory Roles of Herbal Extracts on the Growth, Immune System, and Reproduction of Fish(MDPI, 2021) Ahmadifar, Ehsan; Pourmohammadi Fallah, Hamideh; Yousefi, Morteza; Dawood, Mahmoud A. O.; Hoseinifar, Seyed Hossein; Adineh, Hossein; Yılmaz, SevdanThe crucial need for safe and healthy aquatic animals obligates researchers in aquaculture to investigate alternative and beneficial additives. Medicinal herbals and their extracts are compromised with diverse effects on the performances of aquatic animals. These compounds can affect growth performance and stimulate the immune system when used in fish diet. In addition, the use of medicinal herbs and their extracts can reduce oxidative stress induced by several stressors during fish culture. Correspondingly, aquatic animals could gain increased resistance against infectious pathogens and environmental stressors. Nevertheless, the exact mode of action where these additives can affect aquatic animals’ performances is still not well documented. Understanding the mechanistic role of herbal supplements and their derivatives is a vital tool to develop further the strategies and application of these additives for feasible and sustainable aquaculture. Gene-related studies have clarified the detailed information on the herbal supplements’ mode of action when administered orally in aquafeed. Several review articles have presented the potential roles of medicinal herbs on the performances of aquatic animals. However, this review article discusses the outputs of studies conducted on aquatic animals fed dietary, medicinal herbs, focusing on the gene expression related to growth and immune performances. Furthermore, a particular focus is directed to the expected influence of herbal supplements on the reproduction of aquatic animals.