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    Revealing Seed Coat Colour Variation and Their Possible Association with Seed Yield Parameters in Common Vetch (Vicia sativa L.)
    (Wiley, 2016) Tiryaki, Gulgun Yildiz; Cil, Abdullah; Tiryaki, Iskender
    The seed coat colour variation of 70 common vetch genotypes were determined by using uniform colour scale (L*a*b*) and their possible correlation with seed yield parameters including the number of pods per plant, the number of seeds per pod, pod dimension, and seed yield (kg/da) was determined. The results revealed presence of highly significant (p < 0.01) variations for both the seed yield and the seed coat colour parameters measured. The number of pods per plant, the number of seeds per pod, and seed yield ranged from 5.8 to 16.03, from 5.2 to 7.66, and from 143.37 to 531.1, respectively. The lightness value varied from 19.00 to 40.28 while chromaticity a* and b* values ranged from -0.16 to 8.99 and from 0.79 to 22.11, respectively. The highest correlation coefficients were determined between b* and L* (r = 0.73), and a* and L* (r = 0.55). The seed coat colour traits and seed yield parameters generally showed weak negative correlations. Seed yellowness (b*) and seed yield had correlation coefficient of -0.25, while correlation between L* and seed yield was determined as -0.23. The results indicated that lightness and yellowness of seed coat may be used as an important parameter to prescreen high yield genotypes of common vetch.
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    Öğe
    Sequence-related amplified polymorphism and inter-simple sequence repeat marker-based genetic diversity and nuclear DNA content variation in common vetch (Vicia sativa L.)
    (Cambridge Univ Press, 2016) Cil, Abdullah; Tiryaki, Iskender
    Genetic diversity of 30 common vetch (Vicia sativa L.) lines and cultivars obtained from various resources or collected from natural flora of Turkey was evaluated by using 55 sequence-related amplified polymorphism (SRAP) and five inter-simple sequence repeat (ISSR) primer sets, and their nuclear DNA contents were determined by flow cytometer. A total of 188 polymorphic loci were detected, with an average of 3.62 loci per primer. The percentage of polymorphic loci was 82.1%. The polymorphism information content values ranged from 0.12 to 0.96, with an average of 0.63. The genetic distance coefficients were in the range of 0.112-0.627. Cluster analysis revealed that the 27 lines and three cultivars could be divided into two main groups. No polyploidy was detected within any vetch lines tested while significant (P<0.0001) nuclear DNA content differences were determined. The present study revealed that fast and accurate fingerprinting analysis could be done using SRAP and ISSR markers, which indicated existing significant variation among common vetch lines and cultivars.