Yazar "Cicekliyurt, Meliha Merve" seçeneğine göre listele

Listeleniyor 1 - 2 / 2
  • [ X ]
    Öğe
    Investigating the cytotoxicity and genotoxicity of Vortioxetine with in vivo and in silico methods
    (Nature Portfolio, 2025) Cicekliyurt, Meliha Merve; Inan, Pinar; Gunay, Melih; Akkus, Gulsum; Cicekliyurt, Altug
    Vortioxetine is a multimodal antidepressant with reported benefits on cognitive functioning, social functioning, and fatigue, however its potential genotoxic risks have not been adequately studied. Therefore, this study aims to investigate the cytotoxicity and genotoxicity of vortioxetine in vivo and in silico. Genotoxic effects were assessed using Chromosome aberration (CA) and micronucleus (MN) assays in cultured human peripheral blood lymphocyte. Cytotoxicity was evaluated through Mitotic index (MI), and DNA interaction was analysed by UV titration and agarose gel electrophoresis. In silico analyses were performed with Attraction Cavities and AutoDock Vina methods. Experimental results demonstrate that vortioxetine binds to Calf Thymus DNA (CT-DNA) through intercalative interactions and cleaves pBR322 DNA in the presence of hydrogen peroxide. DNA binding studies indicated that groove binding is the effective interaction between vortioxetine and CT-DNA (Kb: 6.25 x 105 M- 1). It was also supported by molecular docking results, where binding affinities of vortioxetine and escitalopram were - 7.29 and - 7.69 kcal/mol for Attracting Cavities and - 6.01 and - 6.57 kcal/mol for AutoDock Vina. When comparing vortioxetine to escitalopram, both drugs were found to be potentially genotoxic. These findings suggest a potential genotoxic risk with prolonged use and provide valuable insight for clinicians in evaluating long-term safety.
  • [ X ]
    Öğe
    The expression of miR-22, miR-140, and miR-328 in breast cancer tissues: Implications for PET/CT imaging biomarkers
    (Bayrakol Medical Publisher, 2025) Gokce, Elif Ozlem; Cicekliyurt, Meliha Merve; Gokce, Oruc Numan; Cetin, Kenan
    Aim: To evaluate the correlation between microRNA (miRNA) expression and 18F-fluorodeoxyglucose (18F-FDG) uptake in breast cancer patients, focusing on the tumor-suppressor miRNAs miR-22, miR-140, and miR-328, and their potential as diagnostic biomarkers. Material and Methods: Biopsies from 14 female breast cancer patients were analyzed, including both tumor and adjacent healthy tissues. The expression levels of miR-22, miR-140, and miR-328 were quantified using real-time PCR. SUVmax values from PET/CT imaging were obtained to assess metabolic activity in tumor tissues. Statistical analyses were performed to explore correlations between miRNA expression and PET/CT results. Results: Our patients' ages ranged from 38 to 74 years, with a mean of 59.5 years. The levels of miR-22, miR-328, and miR-140 were significantly lower in breast cancer tissues compared to healthy tissues. However, no statistically significant correlations were found between miRNA values and 18-FDG PET/CT SUVmax values. Discussion: While miR-22, miR-140, and miR-328 show promise as potential diagnostic biomarkers for breast cancer due to their significant downregulation in tumor tissues, their expression does not correlate with metabolic activity as measured by SUVmax in PET/CT imaging. Further research is needed to explore the clinical applications of miRNAs in breast cancer diagnosis and prognosis.