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    Could the salivary microbiota be an individual signature?
    (2022) Cakan, Huseyin; Rostamkhil, Zargai; Cevik, Filiz Ekim
    DNA is one of the important pieces of evidence at the crime scene. There may be cases where the DNA is damaged or insufficient. In this case, the criminal can be identified by qualifying microorganisms as evidence. In recent studies, it has been shown that the detection of microbiota communities on the surface of the human body can bring a new perspective to forensic science. In our study, we investigated the importance of the microbiota in the saliva in terms of identification in forensic sciences, by using phenotypic and morphological features as opposed to genotypic identification, and by checking how unique and characteristic features of these determined microorganisms are compared to other people. 60 different swab samples, including the mouth and bitten right index finger, were cultivated in 4 different media (Blood, Chocolate, Endo, and Chrom) from 30 subjects. All reproducible microorganisms found in the saliva of individuals were investigated. According to the data obtained from the mouth and bitten finger; It was determined that alpha-hemolytic streptococci were the most dominant species among these microorganisms. In addition, Neisseria sp bacteria in the salivary microbiota of individuals have been shown to have a significant relationship with smoking and Candida sp with oral hygiene. We believe that this study will present a different perspective to the literature in cases where the DNA procedure obtained from the bite marks of suspects in the criminal investigation is degraded or insufficient, or to obtain a complementary result in addition to genotypic approaches.
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    Öğe
    INVESTIGATION OF POSTMORTEM MICROBIAL DNA DEGRADATION
    (Parlar Scientific Publications (P S P), 2022) Sevim, Ozgur; Cevik, Filiz Ekim; Cakan, Huseyin
    DNA technology has entered our lives in every field as one of the most important scientific developments of this century. DNA analysis: It is widely used to identify suspicious persons in various forensic crimes, to identify unidentified corpses who died due to mass disasters and other reasons. In such cases, unsatisfactory results can be obtained from DNA analysis performed on rotten and decaying tissue samples taken from unidentified corpses coming from the crime scene to the forensic laboratories. This may be due to DNA contamination. The most common causes of DNA damage are enzymes secreted by microorganisms found in the corpse and environment. Microbial enzymes cause DNA damage, insufficient or no PCR amplification, thus irregularities in the peak levels in the DNA electropherogram and the appearance of meaningless and missing peaks. When DNA analysis methods are applied to a postmortem biological sample in which microorganisms multiply, it may be difficult to obtain accurate and precise results due to degradation. The aim of this study is to evaluate the importance of identification of microorganisms (bacteria) that reproduce in postmortem muscle tissues in terms of forensic sciences, to determine whether they prevent DNA analysis, and if so, to determine which bacteria may cause it.