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    Öğe
    Biosynthesis of gold nanoparticles using marine bacteria and Box-Behnken design optimization
    (Taylor & Francis Inc, 2019) Camas, Mustafa; Celik, Fatih; Camas, Anil Sazak; Ozalp, Hasan Baris
    Gold nanoparticles are exciting materials because of their potential applications in optics, electronics, biomedical, and pharmaceutical fields. In recent years, environmentally friendly, low-cost biosynthesis methods with bio-applicable features have continued to be developed for the synthesis of gold nanoparticles. In the present study, an actinobacterial strain was isolated from the Petrosia ficiformis (Poiret 1798) sponge, which was collected from a marine environment, and the gold nanoparticle synthesis was performed for the first time from the bacteria type belonging to the Citricoccus genus. The synthesis conditions were optimized using the Box-Behnken experimental design, with a statistical method that included three independent variables (temperature, time, and mixture ratio) to affect the synthesis at three levels (+1, 0, and -1). Accordingly, the conditions proposed for the biosynthesis of gold nanoparticles at the maximum optical density values that are specific for the Citricoccus sp. K1D109 strain were estimated as 35 degrees C temperature, 24 h, and 1/5 mixture ratio (cell-free extract/HAuCl4 center dot 3H(2)O). When recommended conditions were applied, it was determined that the maximum absorbance of the synthesized gold nanoparticles is 1.258 at 545 nm, and their sizes are in the range of 25-65 nm, according to transmission electron microscopy (TEM) data.
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    Öğe
    Sarcotragus foetidus (Schmidt, 1862) Denizel Süngeri ile Uranyum (VI) Biyosorpsiyonunun Merkezi Kompozit Dizayn ile İstatistiksel Olarak Araştırılması
    (2016) Çelik, Fatih; Camas, Mustafa; Camas, Anil Sazak; Özalp, Hasan Barış
    Mikroorganizmalar ve biyolojik materyaller kullanarak radyonüklitlerin, ağır metallerin, toryum ve uranyum gibi radyoaktif metallerin sulu çözeltilerden geri alınması veya uzaklaştırılması üzerine birçok çalışma vardır. Bu çalışmada, Sarcotragus foetidus (Schmidt, 1862) olarak adlandırılan deniz süngerinin sulu çözeltilerden uranyum alımı istatistiksel olarak araştırıldı. S. foetidus (Schmidt, 1862) deniz süngeri örnekleri Babakale (Ayvacık / Çanakkale) açıklarından toplandı. Uranyum (VI) geri alım denemeleri kesikli yöntem ile gerçekleştirildi. Biyosorpsiyonu etkileyen parametreler Merkezi Kompozit Deneysel Tasarımı (CCD) kullanılarak analiz edildi. Bu çalışma için; pH, sıcaklık, temas süresi ve uranyum başlangıç konsantrasyonu gibi dört bağımsız değişken seçildi. Ana parametrelerden pH, temas süresi ve uranyum başlangıç konsantrasyonu biyosorpsiyon prosesinde istatistiksel olarak anlamlı olarak kabul edildi. Diğer bir yandan; biyosorpsiyon işlemi üzerinde, pH- temas süresi, pH - uranyum başlangıç konsantrasyonu gibi ikili etkileşimler önemli parametreler olarak belirlendi. Biyosorbsiyon prosesinin optimum koşulları; pH:4, sıcaklık: 38 , uranyum başlangıç konsantrasyonu: 20 mg/L ve temas süresi: 31 dakika olarak hesaplandı. Bu koşullar altında biyosorpsiyon verimi % 93.29±2 olarak elde edildi
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    Öğe
    Uranium (VI) Biosorption on Marine Sponge, Sarcotragus foetidus (Schmidt, 1862) and Its Statistical Investigation Using Central Composite Design
    (Central Fisheries Research Inst, 2016) Celik, Fatih; Camas, Mustafa; Camas, Anil Sazak; Ozalp, Hasan Baris
    The biosorption of heavy metals, radionuclides, uranium and thorium for the recovery or removal from aqueous systems using various micro-organisms and biological substances has been investigated by several groups of workers. In this paper, the uptake of uranium (VI) from aqueous systems by a marine sponge, Sarcotragus foetidus (Schmidt, 1862) has been investigated. S. foetidus samples were collected from Babakale/Ayvacik/Canakkale in Turkey. Uranium (VI) uptake experiments were carried out by the batch technique. The affecting parameters were analyzed using Central Composite Design (CCD) as the experimental method. Four independent variables such-pH, temperature, contact time, and initial uranium concentration were selected for this study. The influences of initial pH, contact time, and initial uranium concentration on the biosorption process were considered statistically significant as main effects. On the other hand, interaction effects of pH and contact time, as well as pH and initial uranium concentration, were considered as significant parameters on the biosorption process. The optimum condition of biosorption process was found to be at initial pH: 4, temperature: 35 degrees C, initial uranium concentration: 20 mg/L, and contact time: 31 minutes. At these conditions, the biosorption yield of uranium (VI) ions was obtained to be 93.29 +/- 2%.