Yazar "Cakmakci, Ramazan" seçeneğine göre listele
Listeleniyor 1 - 12 / 12
Sayfa Başına Sonuç
Sıralama seçenekleri
Öğe Analysis of genetic variation and population structure among of oregano (Origanum acutidens L.) accessions revealed by agro-morphological traits, oil constituents and retrotransposon-based inter-primer binding sites (iPBS) markers(Springer, 2020) Karagoz, Halit; Cakmakci, Ramazan; Hosseinpour, Arash; Ozkan, Gutter; Haliloglu, KamilThe genetic associations among 31 oregano (Origanum acutidens (Hand.-Mazz.) Ietswaart) genotypes collected from different provinces of Turkey were determined by comparing their agro-morphological traits, essential oil components and molecular traits. In order to assess the genetic diversity in 9 agro-morphological traits and 9 essential oil components, 10 polymorphic iPBS primers were used. Principle component analysis (PCA) for agro-morphological traits had two axes explaining 81.35% of total variation and PCA for essential oil components had 4 axes explaining 89.67% of total variation. Cluster analysis yielded two main clusters for agro-morphological traits and four main clusters for essential oil components. Present iPBS primers generated 289 alleles. Number of alleles per polymorphic locus varied between 25 and 36 with an average value of 28.40. The average polymorphism ratio was as 98.36%. Polymorphic information content values varied between 0.28 and 0.42. The average number of alleles (ne), Nei's genetic diversity (h) and Shannon's information index (I) were obtained 1.61, 0.37 and 0.55, respectively. The 31 accessions were assembled into three main clusters according to the unweighted pair-group mean average based on iPBS markers. Similarity index value varied between 0.51 and 0.81. Moreover, population structure analysis using Bayesian method showed that all genotypes formed eight sub-population (K = 8) with an average value of 0.3544 for expected heterozygosity and 0.0400 for population differentiation measurements (F-st value). In present study, population structure of 31 oregano genotypes collected from different provinces of Turkey were successfully characterized with the aid of iPBS markers and model-based clustering. It was concluded based on present findings that iPBS markers could reliably be used in genetic diversity and molecular analysis of oregano genotypes.Öğe Characterization of plant growth-promoting rhizobacteria (PGPR) in Persian walnut associated with drought stress tolerance(Nature Portfolio, 2022) Lotfi, Naser; Soleimani, Ali; Cakmakci, Ramazan; Vahdati, Kourosh; Mohammadi, ParisaThere is a lack of information on the rhizosphere of nut-bearing trees where microbial populations can benefit roots and tree growth. The current research aimed at discovering plant growth-promoting rhizobacteria (PGPR) in the rhizosphere of soil samples from around the root zone of six walnut trees, each of which was considered as a genotype, i.e. 'TT1', 'TT2', 'SS2', 'ZM1', 'Chandler' and 'Haward'. The trees grew in different arid and semiarid regions of Iran and Turkey. The strains were isolated and identified based on different morphological and biochemical markers. Drought-stress tolerance was assessed in the case of each isolate through their transfer to culture medium, containing polyethylene glycol (PEG(6000)) at 0 and 373.80 g L-1. Resilient strains were analyzed for measuring their ability to produce siderophore, hydrogen cyanide (HCN), Indole-3-acetic acid (IAA) and Gibberellic acid (GA(3)). In sum, 211 isolates were identified, of which a large number belonged to the Bacillus genus and, specifically, 78% of the strains were able to grow under drought stress conditions. The genus Arthrobacter was only detected in the rhizosphere of 'ZM1', 'Haward' and 'TT1' genotypes. In 4% of the strains, IAA production exceeded 53 mg L-1, while a high level of phosphorus solubility was verified in 6% of the strains. No strain was found to have the capability of producing HCN. The strains were screened for drought-tolerance, which resulted in the discovery of two promising strains, i.e. ZM39 and Cha43. Based on molecular identification through amplification and sequencing of the 16S rDNA gene, these two strains seemed to belong to Bacillus velezensis and Bacillus amyloliquefaciens, respectively. The discovery of new PGPR strains could probably assist walnut trees in improving their mechanisms of adaptation to drought stress.Öğe Comprehensive biochemical insights into the seed germination of walnut under drought stress(Elsevier, 2019) Lotfi, Naser; Soleimani, Ali; Vahdati, Kourosh; Cakmakci, RamazanThe evaluation of physiological and biochemical aspects is essential to identify clues that can assist breeders in selecting Persian walnut genotypes with considerable tolerance to drought stress. This objective can be obtained by analyzing seed biochemical compounds from different genetic resources, e.g. seeds from individual trees that are commonly exposed to drought stress. In the current research, we evaluated the effects of drought stress, as induced by PEG(6000), on the germination of walnut seeds collected from six promising genotypes (i.e. 'SS2', 'TT2', 'TT1', 'ZM1', 'Haward' and 'Chandler'). A photoperiod of 12 h was provided during the experiments, along with an average temperature of 25 +/- 1 degrees C. According to the different indexes of germination, a greater tolerance was observed among three genotypes, namely, 'ZM1', 'Haward' and 'Chandler'. In response to drought stress, the contents of proline and total soluble sugars increased, especially at intense levels of stress, whereas the amount of starch decreased. The levels of antioxidant activity significantly increased by POD, APX, CAT, SOD and LOX enzymes in the radicle and plumule tissues as the water potential reached -1.5 MPa. Three genotypes, namely, TT2', 'TT1' and 'SS2' were susceptible to cell wall damage (i.e. by lipid peroxidation). High levels of Spd and Spm in tolerant genotypes were accompanied by significant increases in the zeatin, endogenous IAA, zeatin riboside and ABA in the seeds. Our results suggest that, under drought stress, seed germination in walnuts can benefit from the accumulation of free Spd in seeds during the phases of germination. However, the accumulation of free Put appeared to be an inhibitory factor which suppressed the germination of walnut seeds.Öğe Dissection of genetic diversity and population structure in oregano (Origanum acutidens L.) genotypes based on agro-morphological properties and start codon targeted (SCoT) markers(Springer, 2022) Karagoz, Halit; Hosseinpour, Arash; Karagoz, Fazilet Parlakova; Cakmakci, Ramazan; Haliloglu, KamilBesides aromatic characteristics of the other oregano species, essential oil of Origanum acutidens has lethal effects on some cancer lines. This study was performed to determine genetic diversity in the 70 oregano (Odostomia acutidens) genotypes based on several agro-morphological traits and start codon targeted (SCoT) markers in the endemic spread zone of O. acutidens. The result of principal component analysis (PCA) revealed that the first two components accounted for 79.56% of the total agro-morphological variation. Cluster analysis based on phenotypic data grouped all investigated genotypes into three main clusters. GGE Biplot analysis indicated that genotype numbers as G35, G11, G24, G10, and G22 with the lowest interaction with different environments were more stable than the others ones. Taking into account all analyses, genotype numbers G36, G12, G10, and G33 were selected as ideal materials. In the molecular section, 10 SCoT primers generated 109 polymorphic fragments. The mean value of polymorphic information content (PIC) was 0.36. The mean number of effective alleles (Ne), Nei's genetic diversity (H), and Shannon's information index (I) were 1.63, 0.38, and 0.57, respectively. The unweighted pair-group method with arithmetic mean (UPGMA) algorithm grouped the 70 investigated genotypes into three main clusters. Mantel test showed no correlation between genetic distance and geographical distance in these individual (r = 0.01, p = 0.69). Moreover, population structure analysis showed that all genotypes formed three sub-populations. In general, population structure of the 70 investigated oregano genotypes collected from different origins of Turkey were successfully characterized with the aid of SCoT markers. Our results suggest that evaluation of genetic diversity and structure analysis in the natural populations could efficiently provide comprehensive information for conservation of endemic and endangered species that can be used in future breeding programs.Öğe DIVERSITY AND METABOLIC POTENTIAL OF CULTURABLE N2-FIXING AND P-SOLUBILISING BACTERIA FROM RHIZOSPHERE OF WILD CROPS IN VAN LAKE BASIN -TURKEY(Pakistan Botanical Soc, 2024) Erman, Murat; Kotan, Recep; Cakmakci, Ramazan; Cig, Fatih; Karagoez, Kenan; Soenmez, Ferit; EL Sabagh, AymanThe diversity of phosphate solubilizing and nitrogen -fixing bacteria from rhizospheric soil samples of different plant species (native wild cereals, beet, stonecrops, onion, rose, raspberry, and 10 other plant genera) grown at four different locations in the Van Lake Basin, Turkey were investigated. A total of 169 rhizosperic soil samples were gathered from these plant species, and over 777 rhizoplane bacteria were haphazardly selected from agar -solidified trypticase soy broth and examined for fatty acid methyl ester (FAME) reports. As a result, 57 bacterial genera were recognised, which 64.8% gone to five genera viz., Bacillus (29.6%), Pseudomonas (9.8%), Stenotrophomonas (9.7%), Paenibacillus (5.7%), Micrococcus (5.1%), and Arthrobacter (4.9%). Approximately 56.8% of bacteria were found to be gram -positive (GPB), while 43.2% as gram -negative bacteria (GNB). In the of total GPB, 56.3% were N2 -fixing bacteria and 46.6% were P-solubilising bacteria, while in the case of GNB, 43.7% were N2 -fixing bacteria and 53.4% were P-solubilising bacteria. A total of 651 bacteria were isolated from the rhizospheric samples of the 20 plant genera, and subjected to further analysis. Of the 651 isolates, 542 were able to fix nitrogen, 279 were able to solubilize phosphates, and 247 isolates could fix both nitrogen and solubilize phosphates all at once. Bacillus, Pseudomonas, Stenotrophomonas, Paenibacillus, Micrococcus, Serratia, and Pantoea genera were the most prominent N2 -fixing and P-solubilising groups. Additionally, B. megaterium, B. atrophaeus, B. cereus, B. pumilus, B. licheniformis, B. subtilis, Pb. polymyxa, Pb. macerans, Pb. macquariensis, Bb. choshinensis, Ste. maltophilia, Ps. fluorescens, Ps. putida, Ps. stutzeri, Pn. agglomerans, Mic. luteus, Rco. erythropolis, Kocuria rosea, Csb. Meningosepticum, and Serratia fonticola were the mainly frequent P-solubilising and N2 -fixing species in the native rhizosphere soils of plants.Öğe Effect of Different Plant Growth-Promoting Rhizobacteria on Biological Soil Properties, Growth, Yield and Quality of Oregano (Origanum onites L.)(Mdpi, 2023) Cakmakci, Ramazan; Haliloglu, Kamil; Tuerkoglu, Aras; Ozkan, Guller; Kutlu, Meral; Varmazyari, Atefeh; Molnar, ZoltanIntensive agriculture uses continuous chemical fertilizers to increase crop yields, but excessive use of fertilizers leads to environmental pollution, permanent changes in physicochemical conditions in soil ecology, deterioration of soil biological health, leaching of nutrients, surface and groundwater pollution and eutrophication. Plant growth-promoting rhizobacteria (PGPR) are becoming increasingly important for ensuring crop safety, increasing nutrient uptake and output, lowering fertilizer costs, preventing environmental contamination and promoting sustainable agriculture and agricultural resources. Therefore, the purpose of this study was to identify and evaluate the effects of fifteen bacteria strains that were isolated from various acidic rhizospheric soils as biofertilizers on soil biological properties. Growth, yield and quality traits were analyzed, and various PGPR were identified using 16S ribosomal RNA of Turkish oregano. Fifteen bacterial inoculations with 1-aminocyclopropane-1-carboxylate (ACC) deaminase, N2-fixing, P-solubilizing and/or IAA-producing genes were used in the experiment, which was carried out in a randomized block design with five replicates (each with three pots) and a control without inoculation. Increased biological activity in soil inoculated with bacteria with multiple traits was confirmed by high C and N content in microbial biomass, urease, dehydrogenase and acid and alkaline phosphatase activities. Essential oil content, oil yield, thymol and carvacrol contents increased by 0.5-40.1%, 5.9-71.9%, 0.07-16.7% and 0.3-9.2%, respectively, as a result of bacterial inoculation. Oil content ranged from 2.02% to 2.83%; carvacrol (66.1-72.2%) was the main constituent, followed by thymol (14.5-16.9%) and linalool (1.38-3.68%). Two large PGPR groups were formed based on genetic distance analysis. Responses were variable and depended on the inoculant strain and the parameters being evaluated. The results indicate PGPR has clear potential for improving the yield of cultivated aromatic and essential oil plants, such as oregano.Öğe Effects of Root Plant Growth Promoting Rhizobacteria Inoculations on the Growth and Nutrient Content of Grapevine(Taylor & Francis Inc, 2018) Erdogan, Ummugulsum; Turan, Metin; Ates, Fadime; Kotan, Recep; Cakmakci, Ramazan; Erdogan, Yasar; Kitir, NurguelThe objective of this study was to evaluate the effects of seven nitrogen (N-2)-fixing and/or phosphorus (P)-solubilizing and siderophore-producing microorganism based bio-fertilizers in single and triple strain combinations isolated from the acidic rhizospheric soil of native tea, grapevine, and wild red raspberries. As a result of this study, bacterial efficiency was found to be variable and depended on the bacterial strains and evaluated growth parameters. Plant growth-promoting rhizobacteria (PGPR) has improved macro- and micro-nutrient concentrations in grapevine leaves, and stimulated plant growth. Triple inoculation and single inoculation based bio fertilizers were found to stimulate overall plant growth, including shoot and leaf weight, main shoot length, leaf ground index, chlorophyll, nitrogen, zinc and iron content of grapevine cv Italy'. Bio-fertilizers increased the nutrients such as nitrogen, zinc and iron concentrations and consequently increased the chlorophyll content of the leaves.Öğe Foods of the Future: Challenges, Opportunities, Trends, and Expectations(Mdpi, 2024) Cakmakci, Songul; Polatoglu, Bilgehan; Cakmakci, RamazanCreating propositions for the near and distant future requires a design to catch the tide of the times and move with or against trends. In addition, appropriate, adaptable, flexible, and transformational projects are needed in light of changes in science, technology, social, economic, political, and demographic fields over time. Humanity is facing a period in which science and developing technologies will be even more important in solving food safety, health, and environmental problems. Adapting to and mitigating climate change; reducing pollution, waste, and biodiversity loss; and feeding a growing global population with safe food are key challenges facing the agri-food industry and the food supply chain, requiring systemic transformation in agricultural systems and sustainable future agri-food. The aim of this review is to compile scientific evidence and data, define, and create strategies for the future in terms of food security, safety, and sufficiency; future sustainable foods and alternative protein sources; factors affecting food and nutrition security and agriculture; and promising food systems such as functional foods, novel foods, synthetic biology, and 3D food printing. In this review, the safety, conservation, nutritional, sensory, welfare, and potential challenges and limitations of food systems and the opportunities to overcome them on the basis of new approaches, innovative interpretations, future possibilities, and technologies are discussed. Additionally, this review also offers suggestions for future research and food trends in light of future perspectives. This article focuses on future sustainable foods, alternative protein sources, and novel efficient food systems, highlights scientific and technological advances and new research directions, and provides a significant perspective on sustainability.Öğe Isolation and Characterization of Phosphate Solubilizing Bacteria and Effect of Growth and Nutrient Uptake of Maize Under Pot and Field Conditions(Taylor & Francis Inc, 2022) Ates, Ozgur; Cakmakci, Ramazan; Yalcin, Gulser; Taspinar, Kadriye; Alveroglu, VolkanPhosphorus deficiency is one of the main factors for limiting agricultural production. The use of Phosphate Soluble Bacteria (PSB) is an environmentally friendly solution to eliminate phosphorus deficiency. Isolation of PSB and determination of plant growth effects of maize were aimed at this study. For this purpose, 62 phosphate solubilizing rhizobacterial strains isolated from soil and the phosphate solubilization abilities of isolates were determined by laboratory, pot, and field trials. After laboratory trials with 62 strains, pot tests were established with 10 isolates. Inoculation of phosphorus PSB was increased plant height19.38-54.25% and plant dry weight12.32-67.63% under pot experiments. Field trials were conducted between 2014 and 2016 with two PSB (S. marcensens and P. brenneri) according to pot experiments. S. marcensens and P. brenneri increased yield of maize32-33% in 2014 and28-25% in 2015, respectively. Application of P. brenneri increased leaf P, K, and Mg content by 13%, 5%, 9%, and S. marcensens increased the same by 10%, 6%, 17%, respectively. P. brenneri and S. marcensens can be used as biological fertilizers as a promising and sustainable approach to maize productivity in P deficient soils.Öğe The Effect of Auxin and Auxin-Producing Bacteria on the Growth, Essential Oil Yield, and Composition in Medicinal and Aromatic Plants(Springer, 2020) Cakmakci, Ramazan; Mosber, Goltay; Milton, Ada Hazal; Alaturk, Firat; Ali, BabooAromatic plants had been used since ancient times for their preservative and medicinal properties, and to impart aroma and flavor to food. Also their secondary metabolites are economically important as drugs, flavor and fragrances, pharmaceuticals, agrochemicals, dye, and pigments, pesticides, cosmetics, food additives, other industrially biochemical, and also play a major role in the adaptation of plants to their environment. Indole acetic acid-producing rhizobacteria inoculations increase in stomatal density and level of secondary metabolite and have a synergistic effect on monoterpene biosynthesis. Bacterial inoculation significantly affected and increased the chemical composition of essential oil, citronellol, and geraniol content in rose-scented geranium; essential oil composition and total phenolic content in marigold; density, number, and size of glandular trichomes in sweet wormwood and peppermint essential oil components such as geranyl acetate, limonene, and beta-pinene in coriander; oil yield and content in calendula; yield of the herb in hyssop; oxygenated compounds, essential oil content and yield, anethol and changing the chemical composition in fennel; growth, number of glandular trichomes and essential oil yield, root branching and length, and total amount of essential oil, production of monoterpenes such as pulegone, menthol, menthone, menthofuran, and terpineol content, biosynthesis of secondary metabolites in peppermint; growth and essential oil yield in marjoram; glandular hair abundance, essential oil yield, and monoterpene biosynthesis in basil; phellandrene, limonene, borneol, and campor in rosemary; carvacrol, thymol, linalool, and borneol in oregano; and alpha-thujene, alpha-pinene, alpha-terpinene, p-simen, beta-pinene, and gamma-terpinene contents and essential oil yield in summer savory. Inoculation with IAA-producing bacteria medicinal roots increased the valerenic acid in valerian, essential oil and quality in vetiver, curcumin content in turmeric alkaloid and ginsenoside content in ginseng, and inulin content in Jerusalem artichoke.Öğe The Effects of Biofertilizers on Some Physological Responses in Heritage Raspberries(Kahramanmaras Sutcu Imam Univ Rektorlugu, 2020) Balci, Gulden; Keles, Hakan; Cakmakci, RamazanRaspberry, which belongs to the genus Rubus of the Rosacea family, is an important berry fruit with economical potential due to its benefits in terms of human health and wide usage area in industry. In this study, the effects of biological fertilizers (PGPR and mycorrhiza) on some chemical properties of Heritage raspberries were investigated. For this purpose, chlorophyll, anthocyanin, total phenolic content, proline, total carbohydrate levels of plant leaves were evaluated. The highest chlorophyll and anthocyanin contents were obtained from BF5 and BF4 bacterial inoculations (37.24 and 37.85 and 5.58 and 5.14, respectively). The effect of applications on total carbohydrate content was not significant. The highest phenolic and proline contents (2.94 GAE mg g(-1) and 0.091 proline g(-1)) were obtained from BF5 treatment. The results indicated that bacterial applications were the first application in terms of the parameters examined for Heritage raspberry cultivation in the region.Öğe The Variability of the Predominant Culturable Plant Growth-Promoting Rhizobacterial Diversity in the Acidic Tea Rhizosphere Soils in the Eastern Black Sea Region(Adem Yavuz Sonmez, 2019) Cakmakci, RamazanThe purpose of this study was to investigate the diversity of cultivable nitrogen fixing, phosphate solubilising and total bacteria originated from 580 rhizospheric acidic soils samples of tea plants grown at 62 locations. Based on FAME profiles of over 1428 rhizoplane bacteria, 63 bacterial genera were identified with a similarity index > 0.3, but 56.4% of the identified isolates belonged to six genera: Bacillus (37.02%), Pseudomonas (12.67%), Stenotrophomonas (5.71%), Paenibacillus (6.58%), Arthrobacter (4.35%) and Brevibacillus (3.98%). Most of the total, N2-fixing and P-solubilizing bacteria isolated were Gram positive (59.9, 58.8 and 56.3%) and Gram negative constituted only 40.1, 41.2 and 43.7%. Among different groups, Firmicutes, Gammaproteobacteria and Actinobacteria comprised the largest groups contributing to about 50.3 and 46.6%, 30.8 and 32.5%, and 8.3 and 9.6% of the total N2-fixing and P-solubilizing isolates, respectively. B. cereus, P. fluorescens, B. megaterium, S. maltophilia, P. putida, B. licheniformis, B. pumilus, B. subtilis and P. polymyxa were the most frequent N2-fixing and P-solubilizing species in the acidic tea rhizosohere soils. In these studies were evaluated to represent the dominant culturable diversity of diazotrophs and phosphobacteria, and thus potentially beneficial to the growth and survival of tea plants in that specific acidic ecosystem of eastern Black Sea region.
