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    Alterations of Hematological Variations in Rats Exposed to Extremely Low Frequency Magnetic Fields (50Hz)
    (Elsevier Science Inc, 2009) Cakir, Dilek Ulker; Yokus, Beran; Akdag, Mehmet Zulkuf; Sert, Cemil; Mete, Nuriye
    Background and Aims. The aim of this study was to evaluate the possible effects of in vivo exposure to extremely low frequency electromagnetic fields (ELF-EMF) on whole blood parameters (hematological parameters) in rats. Methods. Forty eight female Wistar rats, obtained from the Medical Science Application and Research Center, Dicle University, Turkey in 2004 were divided into four separate groups: two exposed groups (0.97 mT, 50 and 100 days, 3 h/day) and two controls (sham). Results. Eosinophil, hemoglobin and MPV levels significantly decreased in rats that were exposed to EMF for 50 days. When the data for rats exposed for 50 days and 100 days were compared, it was found that MPV levels in rats exposed for 100 days were significantly lower. There was no significant difference in total leukocyte, neutrofil, lymphocyte, monocyte, eosinophil and basophil counts, or in erythrocyte, Hct, MCH, MCHC, RDW, PLT and PDW levels between the exposed and sham-exposed groups. ELF-EMF exposure had no effect on body weight. Also, liver weight did not show any significant difference between groups. Conclusions. Our results indicate that the applied ELF-EMF exposure may induce slight but statistically significant alterations in some hematological parameters of rats, within the physiological range. (C) 2009 IMSS. Published by Elsevier Inc.
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    Assessment of Serum Mineral and Certain Biochemical Variables in Self-Sucking Dairy Cows
    (Medwell Online, 2008) Bademkiran, Servet; Yokus, Beran; Icen, Hasan; Cakir, Dilek Ulker; Kurt, Dogan
    Self-sucking, that is, a cow sucking on her own teats is an important and leading cause of economic loss. However, the causes of self-sucking are virtually unknown, although numerous possible influencing factors, such as feeding management, nutrient deficits, genetic factors and housing systems have been suggested. In this study, our main objective was to investigate the possible effects of mineral levels on self-sucking in dairy cows. Biochemical variables of blood serum were also investigated. Cows which self-sucked had significantly lower serum concentrations of Mn, Co, Zn, P, Na, Cl, K and total protein, compared with the control group. Levels of serum cholesterol, HDL-C, LDL-C and ALP activity were significantly increased in the self-sucking group. There were no significant differences in serum levels of Ca, Mg, Cu, Fe, saturated Fe, iron binding capacity, urea, creatinin, uric acid, total bilirubin, amylase, gamaglutmyl transaminase, lactate dehidrogenase, creatin kinase, creatine kinase-MB, alanine transaminase and aspartate transaminase activities between the self-sucking group and the control group. The findings provide novel information about whether macro and micro element deficiency may cause self-sucking in dairy cows. An evaluation of our results supports the hypothesis that energy deficiency is a possible cause of self-sucking. Although the explanation is not clear, it may be related to decreased Co, Mn and P levels, which are important in carbohydrate and energy metabolism.
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    Association between serum inhibin-B levels and coronary artery disease in aging males
    (Termedia Publishing House Ltd, 2013) Kocoglu, Hasan; Alan, Cabir; Cakir, Dilek Ulker; Malkoc, Ercan; Cosansu, Kahraman; Kinlmaz, Bahadir; Ertung, Yunus
    Introduction: Atherosclerosis is a systemic disorder. It is a frequent leading cause of coronary artery disease (CAD). Similarly, atherosclerotic vascular alterations could lead to testicular arterial blood flow reduction and impairment of testicular function with age. Inhibin-B has been validated as a valuable serum marker of testicular functions and its correlation with testicular volume was shown in some studies done before. The purpose of this study is to investigate the association between serum inhibin-B levels and CAD in elderly men. Material and methods: Between March 2009 and March 2010, fifty-two 50-80-year-old consecutive patients with Gensini score over 20 and ejection fraction (EF) > 50% were included in the study as the CAD group. Fifty healthy men without any cardiac disease history were recruited as the control group. All patients in the CAD group who had indications for coronary artery angiography underwent selective coronary artery angiography. Results: Inhibin-B, total testosterone and testicular volume levels were found to be significantly lower in the CAD group in comparison with the control group (p = 0.004, p < 0.0001, and p = 0.001 respectively). Conclusions: In this study, although no correlation was found in CAD patients between Gensini score and inhibin-B or testicular volume, inhibin-B levels and testicular volume were significantly lower in patients with CAD than in healthy men. In order to fully assess the relationship between serum inhibin-B levels and CAD, multi-centered prospective and longitudinal studies must be done in elderly male patients.
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    Asymmetric dimethyl arginine levels in testicular tissue and blood of rats with induced experimental varicocele
    (Elsevier Espana Slu, 2014) Alan, Cabir; Ertung, Yunus; Cakir, Dilek Ulker; Topaloglu, Naci; Ersay, Ahmet Resit; Basturk, Gokhan
    Introduction: Varicocele is one of the reasons for testicular dysfunction and is frequently known to accompany infertility. The basic pathology of varicocele is the development of endothelial dysfunction. The most important factors in development of endothelial dysfunction are impaired endothelial-linked vasodilatation, increase in free oxygen radicals, reduced synthesis and release of nitric oxide (NO), abnormal vasoconstriction and increased levels of dimethyl arginine. Our aim was to identify and illustrate the relationship between asymmetric dimethyl arginine (ADMA) and NO levels in testicular tissue and plasma of rats with induced experimental varicocele. Materials and methods: Twenty-one adolescent (average 6 weeks) male rats were included in the study and randomly divided into 3 groups. Group 1 (control, n=6) did not undergo any procedure. Group 2 (sham, n=6) had the left renal vein circled proximally but ligation was not performed. Group 3 (varicocele-induced, n=9) had partial ligation of the proximal left renal vein to induce left varicocele. Superoxide dismutase (SOD) enzyme activity and levels of end-products of NO, nitrite and nitrate salts were investigated in testis tissue. Nitrite/nitrate and ADMA levels were investigated in plasma. Histopathological examination was completed with routine hematoxylin-eosine and TUNEL dyes. Results: Tissue SOD and plasma ADMA values were clearly increased in the varicocele group compared to the other groups; tissue and plasma nitrite/nitrate levels were clearly reduced in the varicocele group and this was observed to be statistically significant between the groups. Conclusion: We believe our study has opened an important window on the relationship between infertility observed in varicocele patients and ADMA. We believe that broad-series prospective studies to support this are required. (C) 2014 Asociacion Espanola de Andrologia, Medicina Sexual y Reproductiva. Published by Elsevier Espana, S.L.U. All rights reserved.
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    Capsaicin inhibits cell proliferation by cytochrome c release in gastric cancer cells
    (Sage Publications Ltd, 2014) Meral, Ogunc; Alpay, Merve; Kismali, Gorkem; Kosova, Funda; Cakir, Dilek Ulker; Pekcan, Mert; Yigit, Serbulent
    Capsaicin (trans-8-methyl-N-vanillyl-6-nonenamide) is the principal pungent component in hot peppers. The role of capsaicin in carcinogenesis is quite controversial. Although some investigators suspect that capsaicin is a carcinogen, co-carcinogen, or tumor promoter, others have reported that it has chemopreventive and chemotherapeutic effects. The present study aimed to evaluate the cytotoxicity and chemosensitizing activities of capsaicin alone and on 5-flourouracil (5-FU)-treated gastric cancer cells. In this study, the gastric cancer cell line HGC-27 was used and capsaicin used as a chemosensitizer and 5-flourouracil (5-FU) was used as chemotherapeutic. Cytotoxicity and chemosensitizing activities were analyzed with MTT assay; supernatant levels of LDH and glucose were detected as biochemical markers of cell viability; cytochrome c and AIF were evaluated with western blot; and additionally, wound-healing assays were employed. Results suggested that capsaicin had significant anticancer abilities; such capsaicin were capable of causing multifold decreases in the half maximal inhibitory concentration IC50 value of 5-FU. The continuing controversy surrounding consumption or topical application of capsaicin clearly suggests that more well-controlled epidemiologic studies are needed to evaluate the safety and efficacy of capsaicin use. In summary, the present study demonstrated that capsaicin has the potential to be used for treating gastric carcinoma with 5-FU in vitro.
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    Capsaicin inhibits proliferation and chemosensitizes gastric carcinoma cells to 5-Fluorouracil
    (Amer Assoc Cancer Research, 2014) Kismali, Gorkem; Alpay, Merve; Meral, Ogunc; Kosova, Funda; Cakir, Dilek Ulker; Sel, Tevhide
    [Anstract Not Available]
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    Do 100-and 500-?T ELF magnetic fields alter beta-amyloid protein, protein carbonyl and malondialdehyde in rat brains?
    (Taylor & Francis Inc, 2013) Akdag, Mehmet Zulkuf; Dasdag, Suleyman; Cakir, Dilek Ulker; Yokus, Beran; Kizil, Goksel; Kizil, Murat
    Several studies still state that presently accepted safety standards for extremely low-frequency magnetic fields (ELF-MFs) do not provide adequate protection, and therefore the standards are still open to question. To help resolve this question, the aim of this study was to illuminate the interaction between biomolecules and ELF-MFs by investigating the effect of ELF-MFs on beta-amyloid protein (BAP), protein carbonyl (PC) and malondialdehyde (MDA) in rat brain. For this study, 30 adult male Sprague-Dawley rats were used, which were divided into two experimental groups and a sham exposed group. Rats in two experimental groups were exposed to 100- and 500-mu T ELF-MFs (50 Hz) for 2 h/day for 10 months, which are the generally accepted safety standards for public and occupational exposures. The same procedures were applied to the rats in the sham group, but with the generator turned off. The results of this study showed that neither ELF-MFs used in this study altered BAP level significantly (p > 0.05). However, PC and MDA levels were increased by the exposure to 100- and 500-mu T ELF-MFs (p < 0.0001). In conclusion, both PC and MDA levels were altered by long-term exposure to either 100 or 500 mu T ELF-MF. However, many further and more comprehensive studies will be required to elucidate the interaction mechanisms between ELF-MFs exposure and living organisms.
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    Effect of 900 MHz radio frequency radiation on beta amyloid protein, protein carbonyl, and malondialdehyde in the brain
    (2012) Dasdag, Suleyman; Akdag, Mehmet Zulkuf; Kizil, Goksel; Kizil, Murat; Cakir, Dilek Ulker; Yokus, Beran
    Recently, many studies have been carried out in relation to 900 MHz radiofrequency radiation (RF) emitted from a mobile phone on the brain. However, there is little data concerning possible mechanisms between long-term exposure of RF radiation and biomolecules in brain. Therefore, we aimed to investigate long-term effects of 900 MHz radiofrequency radiation on beta amyloid protein, protein carbonyl, and malondialdehyde in the rat brain. The study was carried out on 17 Wistar Albino adult male rats. The rat heads in a carousel were exposed to 900 MHz radiofrequency radiation emitted from a generator, simulating mobile phones. For the study group (n: 10), rats were exposed to the radiation 2 h per day (7 days a week) for 10 months. For the sham group (n: 7), rats were placed into the carousel and the same procedure was applied except that the generator was turned off. In this study, rats were euthanized after 10 months of exposure and their brains were removed. Beta amyloid protein, protein carbonyl, and malondialdehyde levels were found to be higher in the brain of rats exposed to 900 MHz radiofrequency radiation. However, only the increase of protein carbonyl in the brain of rats exposed to 900 MHz radiofrequency radiation was found to be statistically significant (p < 0.001).In conclusion, 900 MHz radiation emitted from mobile/cellular phones can be an agent to alter some biomolecules such as protein. However, further studies are necessary. © Informa Healthcare USA, Inc.
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    Effects of Lycopene and Vitamine E Administration Over Gastric Mucosal Damage Induced by Aflatoxin B1
    (Medwell Online, 2009) Kurt, Dogan; Saruhan, Berna Guney; Yokus, Beran; Cakir, Dilek Ulker
    In the present study, we aimed to determine, the changes induced by Aflatoxin B-1 (AFB(1)) administration on rat gastric mucosal barrier and gastric mucins alongside revealing the protective effects of lycopene and Vit E. Thirty-five Wistar-Albino male rats weighing 180-220 g, were divided into 7 groups as to include 5 rats in each group: control, lycopene (10 mg/kg/day lycopene for 15 days (Lycopene 10% FS), AFB(1) (single dose of 2.5 mg AFB(1) kg(-1) on the 12th day of the study), Lycopene + AFB(1) (10 mg lycopene/kg/day for 15 days and single dose of 2.5 mg AFB(1) kg(-1) on the 12th day of the study) and Vitamin E + AFB(1) (10 mg/kg/day Vitamin E for 15 days and single dose 2.5 mg AFB(1) kg(-1) on 12th day). Following the sacrifice of study subjects on the 15th day, gastric mucus and phospholipid levels were determined and their stomachs were examined histopathologically. Examination of mucus and phospholipid levels revealed a significant reduction in group 3-5, in which AFB(1) has been applied (respectively, p<0.001, p<0.001). When lycopene and vitamine E groups are compared with the AFB(1) group, a significant elevation was detected in mucus and phospholipid levels (respectively, p<0.001, p<0.001). Whereas, histopathological examination of gastric mucosas of the aflatoxin group showed degenerative changes, gastric mucosas of the control group and the remaining study groups were normal. Histochemically, while neutral mucins were predominant in general structure of stomach, mixed and sialomucins were observed, as well. Particularly acid mucins with suplhate and periodate reactive acid mucins were found to be more predominant in the aflatoxin group compared to control and other groups. Histochemical features of mucins were observed to be consistent with specific functions of the different regions of stomach. Lycopene and vitamine E administrations were found to be protective against the damage induced by aflatoxin on gastric mucosa.
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    Effects of restricted nutrition and flushing on reproductive performance and metabolic profiles in sheep
    (Elsevier, 2022) Yildirir, Mesut; Cakir, Dilek Ulker; Yurtman, Ismail Yaman
    The aim of this study was to assess the impact of dietary restriction during the lactation period on flushing performance and metabolic profiles in sheep. The treatments were performed in a 2 x 2 factorial arrangement with (i) adequate and restricted feeding treatment during lactation and (ii) flushing and control feeding treatment during mating season. Eighty-four Karacabey Merino ewes were divided into two groups for adequate energy (100%) and restricted (70%) at energy requirements for three months of lactation. Each group was also divided into two sub-groups based on flushing diet (AL-F, RL-F; 150%) or control diet (AL-C, RL-C; 100%) during the mating season. Live weight (LW), body condition score (BCS), serum metabolites and hormone concentrations, ovulation rate, estrus performance and lambing performance of ewes were observed. Data from this study showed that average difference (P < 0.001) of LW and BCS between groups were 5.45 kg and 0.54 respectively at the end of lactation. No significant effects of nutritional treatments (P > 0.05) were observed; the average fecundity, litter size and ovulation rates of ewes were 1.30 +/- 0.07, 1.44 +/- 0.06 and 1.53 +/- 0.07 respectively. The onset of estrus was earlier (P = 0.021) in RL-F ewes (7.0 d) than for AL-F, AL-C and RL-C ewes (11.0, 12.2 and 10.9 d respectively). Mean PRG concentration of ewes was lower (P = 0.011) in RL-C group than ewes in AL-C group (1.859 +/- 0.266 and 3.143 +/- 0.272 ng/ml respectively) at the ram introduction day. Serum biochemical variables blood urea nitrogen (BUN), glucose (GLUC), triglycerides (TGL) and very low density lipoprotein cholesterol (VLDL-CHOL) concentrations-decreased (P < 0.05), while non-esterified fatty acid (NEFA), cholesterol (CHOL), high density lipoprotein cholesterol (HDL-CHOL) and low density lipoprotein cholesterol (LDLCHOL) concentrations increased (P < 0.05) in the restricted ewes compared with adequate ewes in the different months. In conclusion, in addition to BCS, blood total protein, BUN, GLUC, NEFA and CHOL can be recommended for testing flock metabolic profiles as specific blood metabolites. Flushing did not cause significant differences in reproductive parameters including the ovulation rate. However, the time required for onset of estrus was found to be significantly shorter after the flushing treatment in restricted group of animals.
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    Evaluation of Serum Creatinine Levels with Reference Change Value in Patients Receiving Colistin Treatment
    (Oxford University Press, 2023) Cinpolat, Havva Yasemin; Alkan, Sevil; Altinisik, Hatice Betul; Cakir, Dilek Ulker; Oguzman, Hamdi
    Objective: In this study, we aimed to evaluate the serum creatinine (SCr) levels with the reference change value (RCV) in patients receiving colistin treatment. Methods: We retrospectively recorded the SCr levels of 47 patients receiving colistin treatment before treatment and on days 3 and 7 after treatment. RCV was calculated with the asymmetrical RCV formula (Z = 1.64, P <. 05). Percent (%) increase in the SCr results of the patients was compared with RCV and values exceeding RCV were regarded as statistically significant. Results: The RCV was calculated as 15.6% for SCr. Compared with pretreatment values, SCr value on day 3 was 32/47 and on day 7 it was 36/47; as these results exceeded RCV, they were considered statistically significant. Conclusion: Use of RCV in the interpretation of results between serial measurements will provide a more rapid and sensitive method when making decisions. © 2023 The Author(s).
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    Evaluation of von Willebrand factor and protein/creatinine ratio in idiopathic sudden hearing loss
    (Elsevier Espana Slu, 2023) Cil, Ozge Caglar; Bakirdogen, Serkan; Cakir, Dilek Ulker; Gul, Hasan; Oymak, Sibel
    Objective: The objective of this study was to evaluate the levels of von Willebraund Factor (vWF) in plasma and the protein/creatinine ratio in urine in patients with idiopathic sudden acute hearing loss, which we think to be caused by epithelial dysfunction. Materials-Methods: Thirty patients with a sudden hearing loss and thirty healthy individuals were included in the study. Before the treatment, blood and urine were collected from the patients and the control group to investigate the levels of the protein/creatinine ratio and the levels of vWF. The test results of the patients group were compared with those of the control group. Results: We found that the levels of vWF increased in the patient group, which was statistically significant (P <.05). The protein/creatinine ratio in the urine increased in the patient group, but this was not statistically significant (P >.05). In addition, we found that the vWF and urine protein/creatin ratio of the patients who benefited from treatment were lower than those who did not benefit. Conclusions: This study showed that sudden sensorineural hearing loss may result from endothelial dysfunction. However, more studies that include more patients are needed in order to support this. (c) 2022 Published by Elsevier Espana, S.L.U. on behalf of Sociedad Espanola de Otorrinolaringolog ' ia y Cirug ' ia de Cabeza y Cuello.
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    Extremely low frequency magnetic fields cause oxidative DNA damage in rats
    (Taylor & Francis Ltd, 2008) Yokus, Beran; Akdag, M. Zulkuf; Dasdag, Suleyman; Cakir, Dilek Ulker; Kizil, Murat
    Purpose: To detect the genotoxic effects of extremely low frequency (ELF) -magnetic fields (MF) on oxidative DNA base modifications [8-hydroxyguanine (8-OH-Gua), 2,6-diamino-4-hydroxy-5-formamidopyrimidine (FapyGua) and 4,6-diamino-5-formamidopyrimidine (FapyAde)] in rat leucocytes, measured following exposure to ELF-MF. Materials and methods: After exposure to ELF-MF (50Hz, 100 and 500 mu T, for 2 hours/day during 10 months), DNA was extracted, and measurement of DNA lesions was achieved by gas chromatography/mass spectrometry (GC/MS) and liquid chromatography/mass spectrometry (LC/MS). Results: Levels of FapyAde, FapyGua and 8OHdG in DNA were increased by both 100 mu T and 500 mu T ELF-MF as compared to a cage-control and a sham group; however, statistical significance was observed only in the group exposed to 100 mu T. Conclusion: This is the first study to report that ELF-MF exposure generates oxidatively induced DNA base modifications which are mutagenic in mammalian cells, such as FapyGua, FapyAde and 8-OH-Gua, invivo. This may explain previous studies showing DNA damage and genomic instability. These findings support the hypothesis that chronic exposure to 50-Hz MF may be potentially genotoxic. However, the intensity of ELF-MF has an important influence on the extent of DNA damage.
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    Genetic influence on urinary vitamin D binding protein excretion and serum levels: a focus on rs4588 C>A polymorphism in the GC gene
    (Frontiers Media Sa, 2023) Dogan, Durmus; Ozcan, Eda Gul; Cakir, Dilek Ulker; Silan, Fatma
    IntroductionVitamin D binding protein (VDBP) plays a crucial role in vitamin D transport and metabolism. The rs4588-A polymorphism of the GC gene, encoding VDBP, has been associated with altered serum VDBP and 25-hydroxyvitamin D (25OHD) levels. However, the mechanisms underlying these effects remain unclear. We aimed to investigate the relationship between urinary VDBP excretion and serum VDBP and 25OHD levels in individuals with and without the rs4588-A allele.MethodsA cross-sectional study was conducted on 109 children (mean age: 11.96 years) to explore the impact of rs4588-A on vitamin D metabolism and urinary VDBP excretion. Biochemical analyses determined serum 25OHD and VDBP levels, and urinary VDBP-to-creatinine ratio (u-VDBP/Cr). Genotyping for rs4588 SNP was performed using LightSNiP assay. Statistical analyses included correlation, linear regression, and comparison between allele groups.ResultsParticipants carrying the rs4588-A allele exhibited lower serum 25OHD levels compared to non-carriers (median (IQR): 11.85 (3.5) vs. 12.86 (4.9), p = 0.023). However, no statistically significant differences were observed in serum VDBP levels (126.34 +/- 59.3 in rs4588-A vs. 136.49 +/- 51.3 in non-rs4588-A, p = 0.141) or in u-VDBP/Cr (median (IQR): 0.4 (0.35) in rs4588-A vs. 0.386 (0.43) in non-rs4588-A, p = 0.189) between the two allele groups. A significant inverse correlation between u-VDBP/Cr and serum VDBP levels was found only in rs4588-A carriers (r = -0.367, p = 0.024). No such correlation was observed in non-carriers or the entire cohort. A linear regression analysis confirmed the impact of u-VDBP/Cr on serum VDBP levels in rs4588-A carriers (B = -0.269, t = -2.185, p = 0.035).ConclusionIndividuals with the rs4588-A allele in the GC gene had lower serum 25OHD levels. An inverse correlation between urinary VDBP excretion and serum VDBP levels was observed, suggesting a partial role of the renal pathway in altered serum VDBP and 25OHD levels linked to the rs4588-A allele.
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    Increased Levels of Serum Ischemia Modified Albumin in Patients with Ankylosing Spondylitis
    (Clin Lab Publ, 2016) Turkon, Hakan; Gokmen, Ferhat; Cakir, Dilek Ulker; Sehitoglu, Muserref Hilal; Resorlu, Hatice; Doner, Davut; Essizoglu, Ertan
    Background: The aim of the study was to investigate oxidant/antioxidant status by determining serum ischemia-modified albumin (IMA) levels with oxidative/antioxidant parameters in patients with ankylosing spondylitis (AS) compared to the controls. Methods: The serum concentrations of IMA, IMA/albumin ratio (IMAR), malondialdehyde (MDA), total oxidant status (TOS), oxidative stress index (OSI), superoxide dismutase (SOD), glutathione peroxidase (GPx), and total antioxidant capacity (TAC) were measured in 40 AS patients and 35 healthy controls. Results: Mean serum IMA, IMAR, MDA, TOS, and OSI levels were increased in patients with AS when compared to the control group (p < 0.05 for all). Serum levels of SOD and GPx were significantly lower in the patient group than in the healthy subjects (p < 0.001 for both). Serum TAC levels were decreased in patients with AS compared to the controls but the statistical difference was not significant. Serum IMA levels were found to be positively correlated with BASDAI, BASFI, BASMI, and ASDAS-CRP (r = 0.356, r = 0.370, r = 0.412, r = 0.353, respectively, and p < 0.05 for all). IMAR values showed significant correlations with BASFI, BASMI, and ASDAS-CRP (r = 0.351, p = 0.026; r = 0.400, p = 0.010; and r = 0.379, p = 0.016, respectively). Conclusions: Depletion in antioxidant systems and overproduction of free radicals leading to formation of the oxidative stress may play a role in the development of AS. Increased levels of IMA might provide important contributions to the underlying oxidative stress in AS.
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    ISCHEMIA-MODIFIED ALBUMIN AS AN OXIDATIVE STRESS BIOMARKER IN OBESE CHILDREN
    (Nobel Ilac, 2015) Topaloglu, Naci; Yildirim, Sule; Tekin, Mustafa; Binnetoglu, Fatih Koksal; Cakir, Dilek Ulker; Erdem, Fehime; Cevizci, Sibel
    Objective: Ischemia modified albumin (IMA) is a marker that is considered as an oxidative stress biomarker. In this study we investigated the IMA levels, an oxidative stress marker, in obesity that leads to free radical formation, in children. Material and Method: Fifty obese and fifty normal weighted children as a control were included in the study. Blood samples were collected for biochemical parameters and serum IMA values. Results: Serum IMA levels were significantly higher in obese group than non-obese group (p<0.001). Median and minimum-maximum IMA values of obese and control groups were 930 (min: 673-max 1332) and 864 (min: 496-max: 1068) respectively. Conclusion: IMA may be used as an important marker to show oxidative stress in childhood obesity.
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    Myelin basic protein and ischemia modified albumin levels in acute ischemic stroke cases
    (Professional Medical Publications, 2015) Can, Serdar; Akdur, Okhan; Yildirim, Ahmet; Adam, Gurhan; Cakir, Dilek Ulker; Karaman, Handan Isin Ozisik
    Objective: To investigate early diagnostic effects of serum myelin basic protein (MBP) and ischemic modified albumin (IMA) levels in patients with ischemic stroke. Methods: Fifty patients who presented to an emergency service with acute ischemic stroke between June 2013 to March 2014 were evaluated with the National Institute of Health Stroke Scale (NIHSS) and diffusion-weighted magnetic resonance imaging (MRI). Thirty four healthy cases were included as control group. All patients' serum IMA and MBP level were assessed. Results: Mean IMA value was 0.52 +/- 0.25 cases with acute ischemic stroke and serum IMA levels were significantly higher than the control group (p<0.01). No statistical significance was observed between acute ischemic stroke group and control group related to the MBP serum levels (P>0.05). Statistically significant correlation was detected between the volumes of diffusion restriction on MRI and NIHSS score (P=0.002,r=0.43) and IMA (P=0.015,r=0.344) levels. Conclusions: We have found that serum IMA levels are elevated in acute ischemic stroke cases and these levels are correlated with the ischemic tissue volume. MBP levels do not increase in early period of stroke cases.
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    Nitric oxide and asymmetric dimethyl arginine (ADMA) levels in an experimental hydronephrotic kidney caused by unilateral partial ureteral obstruction
    (Brazilian Soc Urol, 2016) Alan, Cabir; Kurt, Hasan Anil; Topaloglu, Naci; Ersay, Ahmet Resit; Cakir, Dilek Ulker; Basturk, Gokhan
    Aim: Our aim is to measure asymmetric dimethyl arginine and nitric oxide levels in rats with induced unilateral acute ureteral obstruction to research the effects on the kidney. Material and Methods: The study included 21 adolescent (average age 6 weeks) Sprague-Dawley male rats weighing between 240-290g divided at random into 3 groups. Group-1: Control group (n= 6): underwent no procedures. Group-2: Sham group ( n= 6): underwent the same procedures as the experimental group without ureter and psoas muscle dissection. Group-3: Group with induced partial unilateral ureteral obstruction (n= 9). All rats were sacrificed after 12 weeks. Superoxide dismutase enzyme activity and nitrite and nitrate salt levels were measured in renal tissue. Plasma nitrite-nitrate and ADMA levels were examined. Results: In the experimental group histopathological changes observed included renal pelvis dilatation, flattened papillae, sclerotic glomerulus and fibrosis. In the experimental group tissue SOD and blood ADMA levels were higher than the control and sham groups (p< 0.05) while tissue NO and plasma NO values were lower than in the sham and control groups (p< 0.05). Conclusion: Oxidative stress and disruption of NO synthesis play an important role in renal function and histopathological changes after obstructive renal disease. To prevent renal complications developing after obstructive nephropathy we believe that a new strategy may be research on reducing ADMA.
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    Piperlongumine inhibits cell growth and enhances TRAIL-induced apoptosis in prostate cancer cells
    (Wolters Kluwer Medknow Publications, 2020) Kismali, Gorkem; Ceylan, Ahmet; Meral, Ogunc; Alpay, Merve; Kosova, Funda; Cakir, Dilek Ulker; Yurdakok-Dikmen, Begum
    Objective: To investigate whether piperlongumine can sensitize prostate cancer cells to tumor necrosis factor-related apoptosis- inducing ligand (TRAIL) and trigger apoptosis in prostate cells. Methods: Human prostate cancer cell lines PC3, LNCaP, and VCaP were cultured with piperlongumine and TRAIL. Then, cell proliferation, migration, caspase activation, apoptotic protein expressions, and death receptor expressions were measured. Results: Piperlongumine inhibited cell proliferation at low doses (<10 mu M) alone and in combination with TRAIL (25 ng/mL), induced apoptosis, and suppressed cyclooxygenase activation. Additionally, piperlongumine induced expression of death receptors which potentiated TRAIL-induced apoptosis in cancer cells but did not affect decoy receptors. Piperlongumine also downregulated tumor cell-survival pathways, inhibited colony formation and migration of cancer cells alone or in combination with TRAIL. The combination of piperlongumine with TRAIL was found to be synergistic. Conclusions: Our findings indicate that piperlongumine can sensitize cancer cells to TRAIL through the upregulation of death receptors and can trigger apoptosis with the downregulation of anti- apoptotic proteins.
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    Protective effect of syringic acid on kidney ischemia-reperfusion injury
    (Taylor & Francis Ltd, 2016) Sancak, Eyup Burak; Akbas, Alpaslan; Silan, Coskun; Cakir, Dilek Ulker; Turkon, Hakan; Ozkanli, Sidika Seyma
    The objective of the present study was to determine whether preischemic administration of syringic acid (SA) would attenuate renal ischemia-reperfusion injury (IRI). Rats were divided into three groups: Sham group; IR group; and IR + SA group. The effects of SA were examined using biochemical parameters including serum ischemia-modified albumin (IMA), total antioxidant status (TAS), total oxidant status (TOS), oxidative stress index (OSI), tissue superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT) and malondialdehyde (MDA). The apoptosis status and histopathological changes were evaluated. After calculating the score for each histopathological change, the total score was obtained by summing all the scores. In the SA group, MDA, IMA, TOS, and OSI decreased significantly compared to the IR group. After SA administration, the increase in GPx activity was found to be significant. Apoptosis decreased significantly in the SA group compared with the IR group. The total score significantly decreased after administration of SA. Taken together, our findings suggest that SA preconditioning is effective in reducing tissue damage induced in kidney IRI. Renal histology also showed convincing evidence regarding the protective nature of SA.