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Öğe A new organ preservation solution for static cold storage of the liver. Amniotic fluid(Acta Cirurgica Brasileira, 2019) Buyuk, Basak; Demirci, Tuba; Adali, Yasemen; Eroglu, Huseyin AvniPurpose: To evaluate the effect of amniotic fluid in liver preservation in organ transplantation, and compare it with standard preservation solutions. Methods: The groups consisted of Group 1: Ringer Lactate (RL) group, Group 2: HTK group, Group 3: UW group, Group 4: AF group. The livers of rats from Group 1, 2, 3, and 4 were perfused and placed into falcon tubes containing RL, HTK, UW, and AF solutions at +4(sic)degrees C, respectively. The tubes were stored for 12 hours in the refrigerator at +4 degrees C. Tissue samples were taken at the 6th and 12th hours for histopathological examinations of the perfused livers, and storage solutions for biochemical analyzes at 6th and 12th hours. Results: AF was shown to maintain organ viability by reducing the number of cells undergoing apoptosis. Histopathological changes such as sinusoidal dilatation, hydropic degeneration, and focal necrosis were found to be similar to the groups in which the standard organ preservation solutions were used. Additionally, the results of INOS, IL-10, and TNF-alpha, which were evaluated immunohistochemically, have been shown to be similar to the UW and HTK groups. Conclusions: AF provided conservation similar to UW and HTK in the 12-hour liver SCS process. The fact that apoptosis values are comparable to standard preservation solutions supports the success of AF in the cold storage of the liver.Öğe Adalimumab mitigates ovarian ischemia-reperfusion injury in rats by regulating oxidative stress, apoptosis and resolution of inflammation(Wiley, 2019) Beyazit, Fatma; Buyuk, Basak; Turkon, Hakan; Elmas, Sait; Uzun, MetehanAim Ovarian torsion is a rare but an important reason of acute lower abdominal pain in women and associated with serious morbidity and mortality, if not treated promptly. The aim of this study was to evaluate the effects of an antitumor necrosis factor-alpha antibody on ovarian torsion in a rat model of ischemia-reperfusion (I/R) injury. Methods Forty female Wistar Albino rats were used in the present study. The rats were randomly divided into four groups: group I (sham), group II (I/R), group III (I/R + isotonic saline) and group IV (I/R + adalimumab). The I/R model was induced by torsion of both ovaries. Immunohistochemical staining for interleukin-1 beta (IL-1 beta), nuclear factor-kappa B (NF-kappa B), and inducible nitric oxide synthase was performed. Tissue and serum oxidative stress markers in conjunction with apoptotic index (AI) with the terminal deoxynucleotidyl transferase dUTP nick end labeling method were also calculated. Results Tissue total oxidant status, oxidative stress index and nitric oxide values were significantly decreased, and tissue total antioxidant status was found to be increased in group IV. Inflammation, vascular congestion and hemorrhagia were significantly lower in adalimumab-treated group. Serum oxidative stress markers and tissue malondialdehyde levels did not differ in study groups. The AI was significantly increased in groups 2 and 3. Adalimumab treatment significantly decreased the AI. Conclusion Adalimumab therapy in rats attenuated I/R induced ovarian injury, possibly suppressing inflammation, inhibiting oxidative stress, and altering apoptotic pathways.Öğe An immunohistochemistry and histopathological study of ankaferd blood stopper in a rat model of cervical inflammation(Assoc Medica Brasileira, 2019) Beyazit, Fatma; Buyuk, BasakOBJECTIVE: Ankaferd Blood Stopper (ABS) is a medicinal plant extract used topically as a hemostatic, anti-inflammatory, and anti-oxidant agent. Its cytoprotective effect mainly depends on its pleiotropic properties by modulating inflammatory mediators such as IL-1 beta, IL-6, and TNF-alpha. This study aims to test the possible therapeutic effect of ABS in the treatment of erosive and inflammatory conditions occurring in the uterine cervix. METHODS: Twenty-four female Wistar Albino rats were used in the present study. Trichloracetic acid was applied intravaginally to establish an experimental rat model of cervicitis. The rats were randomly divided into three groups: group I (injury), group II (injury+isotoinc saline), and group III (injury+ABS). After 3 estrous cycles of ABS and isotonic saline treatment, the amount of inflammation, vascular congestion and erosion were evaluated in the cervical tissues by using a modified semi-quantitative scale of 0-3. Immunohistochemical staining with monoclonal antibodies against IL-1 beta was also performed. RESULTS: Compared with group I and II, the ABS group showed the least inflammatory cell infiltration, vascular congestion and cervical erosion, compared with the ABS group prominent IL-1 beta staining observed in group I and group II. CONCLUSION: Our data suggest that ABS is a highly effective alternative to induce normal cervical epithelium and can be used safely in the treatment of cervical inflammation with or without cervical erosion.Öğe CAN AMNIOTIC FLUID BE AN ALTERNATIVE ORGAN PRESERVATION SOLUTION FOR COLD RENAL STORAGE?(Asoc Regional Dialisis Trasplantes Renales, 2020) Buyuk, Basak; Demirci, Tuba; Adali, Yasemen; Eroglu, Huseyin AvniIntroduction: Kidney-transplantation is a life-saving treatment option for patients with chronic renal failure. Preserving the viability of the organ from the removal of the organ until transplantation into the recipient is one of the most essential factors affecting postransplant success. Kidney tissue is exposed to ischemia following removal of the organ from the donor, initiating some cellular events. Amniotic fluid (AF) was previously reported as a preservation solution for the liver, but not for the kidney yet. The aim of this study is to investigate the effectiveness of AF as a preserving solution for rat kidneys compared with the University of Wisconsin (UW) and Histidine-Tryptophan-Ketoglutarate (HTK), which are reported to be the most commonly used and preferred preserving solutions. Methods: Forty male Wistar albino rats were used in this study in four experimental groups. Group 1: Ringer Lactate (RL, Control) group, Group 2: HTK group, Group 3: UW group, and Group 4: AF group. A midline incision was performed, and the renal artery was isolated under ketamine and xylazine anesthesia. Solutions relevant for groups (cooled to + 4 degrees C) were used for kidney perfusion. Nephrectomy was applied, and the removed kidneys were placed into + 4 degrees C standard organ storage solution and stored at + 4 degrees C for 12 hours. After 12 hours of storage, samples from the kidney tissues were fixed in 10% neutral buffered formalin. Histopathological, immunohistochemistry evaluation and apoptosis detection via TUNEL method were performed. Results: The results of the AF group were close to those of the UW and HTK groups. Tubular necrosis and vacuolization were high in the RL solution group when compared to the other experimental groups. Immunohistochemistry staining for all three markers (TNF-alpha, IL-18, and iNOS) was decreased in the amniotic fluid group, similar to the UW and HTK groups. Also, the number of apoptotic cells was decreased in the AF group compared to control. Conclusions: UW, HTK, and AF had similar and higher protective effects compared to the RL solution. Thus, AF may be used as an inexpensive and readily available alternative natural tissue preservation solution.Öğe Drug-eluting Vein Graft with Acetylsalicylic Acid-Ticagrelor- Unfractionated Heparin Complex Inhibits Early Graft Thrombosis(Galenos Publ House, 2020) Aksit, Ercan; Kurt, Tolga; Buyuk, Basak; Cokkalender, OmerBackground: Bypass graft surgery remains to be an important treatment option for left main and multivessel coronary artery disease. Approximately 2% of saphenous vein grafts are lost immediately after the coronary artery bypass graft operations and 12% in the first month due to thrombosis. Aims: To administer one anticoagulant and two antiplatelet agents in a way that locally affects the vein graft before the bypass operation and to thereby analyse their effects on early graft thrombosis. Study Design: Animal experimentation. Methods: Since ticagrelor was used locally for the first time in this study; its efficacy in combination with other drugs (acetylsalicylic acid, acetylsalicylic acid and ticagrelor, and acetylsalicylic acid + ticagrelor unfractionated heparin) was examined on rats including control (untreated) and sham (pluronic gel) group (n=14 for each group). Before the tunica adventitia layer of the femoral veins was bypassed to the femoral artery, it was coated with the drug-eluting pluronic F-127 gel. The presence or absence of thrombus in the vein graft samples was recorded under light microscopy. In vein graft preparations where thrombus was detected, the thrombus area (mu m(2)) was calculated using the Axiovision software. Immunohistochemical staining was performed with the anti-rat von Willebrand factor polyclonal antibody kit. Results: The number of preparations containing thrombus was significantly lower in the acetylsalicylic acid + ticagrelor + unfractionated heparin group than in the acetylsalicylic acid, control, and sham groups, according to the comparisons made on the Is/ and 3rd days (p=0.00I and 0.02. respectively). von Willebrand factor staining was significantly lower in the acetylsalicylic acid + ticagrelor + unfractionated heparin group than in the other groups on the 3rd day (p=0.005). Conclusion: Locally effective acetylsalicylic acid-ticagrelor-unfractionated heparin complex has been shown to significantly reduce thrombus formation in vein grafts in this experimental model. Local administration of these drugs, which are routinely administered orally just before stent implantations, on the vein graft before the bypass is performed can prevent the loss of vein grafts due to thrombus, thereby reducing the mortality and morbidity of these patients.Öğe Effectiveness of Brain Protection With Histidine-Tryptophan-Ketoglutarate Solutions(Forum Multimedia Publishing, Llc, 2020) Oguz, Sonay; Asgun, Halil F.; Buyuk, BasakBackground: Tissue-protective solutions increase resistance of cells to ischemic conditions. Especially in carotid and aortic arch surgeries where the brain perfusion is at risk, these solutions may be beneficial to prevent ischemic brain damage. This study was designed to demonstrate the effectiveness of histidine-tryptophan-ketoglutarate (HTK) solution in increasing resistance of brain tissue to ischemic conditions. Methods: Three separate randomized groups were created, each consisting of eight rabbits. The groups were called the ischemia, HTK and sham groups, respectively. In the ischemia group, temporary brain ischemia was created for 15 minutes by placing clamps on the bilateral subclavian and common carotid arteries. Then the clamps were removed, and the brain was reperfused for 30 minutes. In the HTK group, HTK solution was sent to the brain through the internal carotid artery before the same ischemia-reperfusion protocol was applied. Histopathological analyses using a visual scoring system to assess the degree of ischemic changes and the apoptotic cell index by TUNEL test were performed in all brain tissue samples. Results: Apoptotic cell indices of the HTK (20.6%) and sham (17.8%) groups were lower than the ischemia group (56.8%) (P < .05). Statistically significant differences were detected between all groups in categorical scores (P < .05). Conclusions: It was shown that less ischemic damage occurs in the brain tissue with the use of HTK solution, and it may be a candidate approach to prevent the brain from ischemic insults during cerebrovascular surgery. Further studies are required to demonstrate its exact effectiveness, in terms of dose, duration, and temperature.Öğe Effects of hyperbaric oxygen treatment on liver and kidney tissue in chronic arsenic toxicity(Undersea & Hyperbaric Medical Soc Inc, 2022) Eroglu, Huseyin Avni; Buyuk, Basak; Oztopuz, Ozlem; Makav, Mustafa; Aydeger, CemreArsenic (As) is a toxic substance that damages the human body through exposure to drinking water. This exposure damages many organs and tissues in the body, especially the liver and kidneys. Hyperbaric oxygen (HBO2) therapy is a treatment method that acts by reducing oxidative stress parameters in tissues with high-pressure oxygen. Based on this, our study aimed to investigate the effectiveness of HBO2 on liver and kidney tissues with chronic arsenic toxicity. In the study 24 male Wistar albino rats (220-300 g, two to three months old) were equally divided into four groups: Control; As; HBO2; and As+ HBO2. All animals were housed in individual cages. The toxicity model was created by adding arsenic to drinking water at a dose of 5 mg/kg/ day for 60 days. HBO2 was applied 2 ATA pressure for 90 minutes a day for five days. At the end of the study, liver and kidney tissues were taken and stored for analysis. In liver tissue, histopathological showed that arsenic reduced inflammatory cell infiltration, sinusoidal congestion, and hydropic degeneration, while HBO2 increased these measures. Similar results were found by TUNEL method. In kidney tissue, both histopathologic and TUNEL method examinations found similar results with the liver: The As group was more damaged than the As+ HBO2 group.Öğe Effects of Melatonin on Aquaporin Channels in Isoproterenolinduced Myocardial Infarction in Rats(Galenos Publ House, 2023) Tufekcioglu, Nezahat Kubra; Buyuk, Basak; Uzun, MetehanAim: Myocardial infarction (MI) commonly results in myocardial edema, but the relationship between aquporin channels (AQP) and the effects of melatonin (MEL) on MI are not well known. Therefore, the aim of the current study was to investigate the effects of MEL on myocardial edema and the change of gene expression level of AQP channels in an experimental MI model. Materials and Methods: In this study 28 Wistar Albino male rats were used. MI model was established with isoproterenol (85mg/kg). Rats were divided into four groups as: control, isoproterenol (ISO), melatonin (MEL), and isoproterenol+melatonin (ISO+MEL). MEL group was administered 10 mg/kg MEL for 7 days. On day 8, electrocardiographic recordings and blood samples were obtained. Rats were then euthanized and left ventricle tissues were obtained. cTnI and CK-MB levels were examined to assess the success of MI model. AQP1, AQP3, AQP4, AQP7, TNF-alpha, BAX and Caspase-3 gene expression levels were determined. Histopathological examination was performed on left ventricle samples for the evaluation of edema and mononuclear cellular infiltration. Results: Histopathological examination and cTnl and CK-MB levels showed that MI model was produced successfully and MEL significantly reduced myocardial edema and decreased AQP1, AQP3, AQP4 and AQP7 gene expression levels. Conclusion: The results show that MEL decreases myocardial edema by reducing AQP channels, suggesting that it could potentially be used to ameliorate the effects of MI.Öğe Hyperbaric oxygen mitigates KIM-1 and inflammatory cytokine levels in kidney transplantation(Undersea & Hyperbaric Medical Soc Inc, 2023) Buyuk, Basak; Oztopuz, OzlemBackground: The aim of this study was to investigate the effect of hyperbaric oxygen (HBO2) administration during cold ischemic time to organs removed from donors before kidney transplantation. A total of 24 rats were divided into three groups: Group 1 was the control group, Group 2 received 60 minutes of HBO 2 at 2.5 atmospheres absolute, and Group 3 received 120 minutes of 2.5 ATA HBO2. The renal artery was entered with a polyethylene catheter and perfused with a standard organ preservation solution. Falcon tubes containing organs obtained from rats in Groups 2 and 3 were placed in a box supported by ice blocks. The temperature was kept constant at 4 degrees C and the box was placed in a pressure tank with 2.5 ATA HBO2. HBO2 was applied for 60 and 120 minutes, respectively. Organ samples were harvested at the end of 24 hours for histopathological evaluation, immunohistochemical analysis of TNF-alpha and IL- 18, TUNEL analysis for apoptosis, and gene expression levels of kidney injury molecule-1 ( KIM-1) and caspase-3. In histopathological examinations, hematoxylin and eosin staining was performed and samples were evaluated for tubular necrosis and vacuolization criteria. Group 2 and Group 3 had significant decreases compared to Group one in this regard. Immunohistochemical staining was performed for TNF-alpha, IL-18, and apoptosis levels; significant decreases were found in Groups 2 and 3. There were significant decreases in Groups 2 and 3 for KIM-1 and caspase-3 gene expression levels compared to Group 1, as well. Thus, it was demonstrated that during the cold ischemic period before kidney transplantation, HBO2 administration to organs removed from donors can reduce apoptotic cell numbers, inflammatory cytokine release, and histopathological damage to the organs as well as decreasing the expression of the KIM-1 gene, which is an indicator of kidney damage.Öğe Hyperbaric oxygen treatment ameliorates gentamicin-induced nephrotoxicity and expression of kidney injury molecule 1 in the rat model(Undersea & Hyperbaric Medical Soc Inc, 2019) Oztopuz, Ozlem; Turkon, Hakan; Sehitoglu, Muserref Hilal; Buyuk, Basak; Uzun, Metehan; Ovali, Mehmet Akif; Demir, UfukIn recent years, hyperbaric oxygen (HBO2) therapy has been considered as an effective method for the treatment of gentamicin (GM)-induced renal toxicity. However, the findings related to the use of HBO2 for GM toxicity are limited and contradictory. The aim of this study is to investigate the protective role of HBO2 on GM-induced nephrotoxicity. For this purpose, Wistar albino rats (n=28) were randomly divided into four equal groups: C, HBO2, GM and GM+HBO2. GM (100 mg/kg, ip) and HBO2 were applied over seven days. On the eighth day blood and kidney tissue samples were harvested. The albumin, creatinine, and urea levels were determined from serum samples. Superoxide dismutase (SOD), glutathion peroxidase (GSH-Px) activities, malondialdehyde (MDA), total antioxidant status (TAS) and total oxidant status (TOS) values were analyzed spectrophotometrically. The relative expression level of TNF-alpha, IL-1 beta and Kim-1 gene were determined by qRT-PCR assays; histopathologic investigation was completed in kidney tissue samples. Serum urea, albumin and creatinine levels significantly increased in the GM group compared to the GM+HBO2 group. For antioxidant parameters the GM+HBO2 group was not statistically different from the C group but was significantly different compared with the GM group. TNF-alpha, IL-1 beta and Kim-1 gene expression levels in the GM group were statistically increased compared to the GM+HBO2 group (p=0.015, p=0.024, p=0.004) respectively. Severe tubular necrosis, epithelial desquamation and mild peritubular hemorrhage were observed in the GM-administrated group, while HBO2 exposure ameliorated these alterations. In conclusion, HBO2 exposure may be defined as a potential method for the prevention of GM-induced renal toxicity.Öğe KNOWLEDGE, ATTITUDE AND BEHAVIOR OF CHEMOTHERAPY PATIENTS ABOUT INTERVENTIONS FOR FERTILITY PRESERVATION: A DESCRIPTIVE SAMPLE STUDY FROM CANAKKALE(Nobel Ilac, 2019) Buyuk, Basak; Oymak, Sibel; Koral, Lokman; Ozerdogan, OzgurObjective: Neoplasm treatments include chemotherapy and radiotherapy methods alternatively in relation to the type of neoplasms. These methods have many side effects for different tissues and organs. One of these side effects is infertility. Infertility is associated with toxic effects of chemotherapeutics to the germ cells. The aim of this study was to investigate the knowledge, attitude and behavior related to fertility preservation among patients who received chemotherapy. Material and Method: This descriptive type epidemiologic research was completed at a University Research Hospital Oncology Clinic after obtaining written permissions from the chief doctor of the hospital and the oncology clinic and ethical board. The study included patients aged 18 and older with neoplasms attending a University Research Hospital Oncology Clinic from November 2016 to April 2017. The data obtained were analyzed with the SPSS 20.0 statistical program. Results: Of 167 people in the study group, 48.5% were female. In our study, 79.1% of patients stated that doctors did not provide information before beginning neoplasm treatment. In our study reproductive technics assisted intervention consultations were requested by 35.3% who reported they were concerned about experiencing reproductive problems after neoplasm diagnosis, 11.4% who were not concerned and 30.0% who didn't remember their emotions about this topic (p<0.05). Conclusion: It is necessary to create awareness among patients of who, where and when to apply about preserving reproduction and to ensure doctors direct these patients to the correct center. With this aim, it may be useful to train oncologists by embryologists about reproductive technics assisted interventions.Öğe Melatonin ameliorates sodium valproate-induced hepatotoxicity in rats(Springer, 2020) Oztopuz, Ozlem; Turkon, Hakan; Buyuk, Basak; Coskun, Ozlem; Sehitoglu, Muserref Hilal; Ovali, Mehmet Akif; Uzun, MetehanValproic acid (VPA) is a anticonvulsant and mood-stabilizing agent used to treat epilepsy in patients of all ages. However, it can cause hepatotoxicity with increased oxidative stress. Melatonin (MEL) is known as antioxidant and antiinflammatory agent. Therefore, the present study designed to investigate the probable protective role of melatonin against VPA-induced liver toxicity. For that purpose, 28 Wistar rats were randomly selected and divided into four groups, namely the Group C (vehicle), VPA (500 mg/kg/day VPA), MEL + VPA (10 mg/kg/day melatonin + 500 mg/kg/day VPA) and MEL (10 mg/kg/day melatonin). The agents were given by oral gavage for 14 days. Blood and liver tissue samples from all the rats were harvested on the 15th day of experiment. Biochemical analyses were conducted on the blood samples. The levels of malondialdehyde (MDA), superoxide dismutase (SOD), alpha glutathione S-transferases (alpha-GST), nuclear factor-kappa B (NF-kappa B), myeloperoxidase (MPO) and changes in gene expression were examined in the liver tissues. Also, liver histopathological analyses were conducted. VPA administration significantly increased the levels of alpha-GST, MDA, NF-kappa B and of IL-1 beta, TNF-alpha gene expression in the liver compared to Group C. Moreover, vacuolization, hydropic degeneration, inflammatory cell infiltration, and sinusoidal congestion were commonly detected in the VPA-treated group along with the highest apoptotic index (TUNEL staining) values. Melatonin administration was revealed to exhibit powerful protective properties at cellular, inflammatory and oxidative level activities against VPA-induced liver toxicity. Therefore, melatonin administration may be used as an adjuvant therapy against to VPA-induced liver toxicity.Öğe Neuroprotective Effects of Piceatannol on Olfactory Bulb Injury after Subarachnoid Hemorrhage (Mar, 10.1007/s12035-023-03306-x, 2023)(Springer, 2023) Akar, Ali; Oztopuz, Rahime Ozlem; Buyuk, Basak; Ovali, Mehmet Akif; Aykora, Damla; Malcok, Umit Ali[Anstract Not Available]Öğe Prevention of rocuronium induced mast cell activation with prophylactic oleuropein rich diet in anesthetized rabbits(Acta Cirurgica Brasileira, 2018) Simsek, Tuncer; Erbas, Mesut; Buyuk, Basak; Pala, Cigdem; Sahin, Hasan; Altinisik, BetulPurpose: The effect of a prophylactic oleuropein-rich diet before anesthesia accompanied by the widely-used steroid-based neuromuscular drug rocuronium on mast cell activation was investigated in the study. Methods: 14 rabbits used in the study. The rabbits in the oleuropein group were given oleuropein-rich extract added to the animals' water at doses of 20 mg/kg oleuropein for 15 days orally. After 15 days, all rabbits in the two groups were given general anesthesia with rocuronium of 1 mg/kg. After 1 day, animals were sacrificed and the liver tissue sections stained with H&E, toluidine blue and tryptase for immunohistochemical study. Results: There was no statistically significant difference between ALT, AST and albumin averages of the oleuropein and control groups (p>0.05). The tryptase average of the control group was higher than the tryptase average of the oleuropein group and this difference was statistically significant (p=0.003). The T. blue average in the oleuropein group was higher than the control group. However, there was no statistically significant difference between groups (p=0.482). Conclusions: Rocuronium adverse effects, like hypersensitivity and anaphylaxis, may limit routine use of this substance. The use of oleuropein reduced the number of inflammatory cells and prevented degranulation.Öğe Protective effect of metformin against lithium-induced cerebral neurotoxicity in rats(2021) Malçok, Ümit Ali; Şehitoğlu, Müşerref Hilal; Buyuk, Basak; Taş, Halil İbrahimNeurotoxic effects are seen in patients using lithium regardless of dose. The neuroprotective activity of metformin used in the treatment of patients with type 2 diabetes mellitus is known. In this study, the protective efficacy of metformin against the neurotoxic effects of lithium was investigated. Neurotoxicity was induced by administering high dose oral lithium (40 mmol/kg) to rats for two weeks. Four groups were formed, with 6 rats in each group. OSI indices were calculated by measuring serum TAS and TOS levels. TAS, TOS, OSI, Gpx ADAM10, and ADAM17 levels were measured in the brain tissue. The neuroprotective activity of metformin was examined histopathologically by calculating the pyknosis scores, vacuolization, and interstitial edema in the hippocampus and cortex tissues. When lithium and metformin were given together, serum levels of TAS, TOS, and OSI were lower than that of the Lithium group (p<0.001), whereas the TAS level in the brain tissue was higher than that of the lithium group (p<0.05). With the concomitant administration of lithium and metformin, ADAM10 levels decreased (p<0.05), whereas ADAM17 and Gpx levels increased (p>0.05). In histopathological evaluation, pyknosis, interstitial edema, and vacuolization scores of the hippocampus and cortex regions increased with lithium alone but were low when lithium was given together with metformin. Levels of the antioxidant molecules Gpx and TAS decreased with lithium, and ADAM10 and ADAM17 proteins, which indicate the strength of intercellular bonding, increased by administering metformin. In conclusion, the toxic effect of lithium on brain tissue was reduced by metformin.Öğe The effect of Oleuropein on liver in experimental allergic rhinitis model(Bayrakol Medical Publisher, 2020) Oztopuz, Ozlem; Coskun, Ozlem; Buyuk, BasakAim: The aim of this study is to investigate the supplemental effect of oleuropein which is the most commonly used corticosteroid in AR (Allergic rhinitis) treatment and to evaluate possible inflammatory-reducing properties in the liver. Material and Methods: The study was carried out on 42 male Wistar rats. The animals were randomly divided into 6 groups including G1 (C), G2 (AR), G3 (AR +SF), G4 (AR+MF), G5 (AR+OLE), G6 (AR+OLE+MF). Tumor necrosis factor-alpha (TNF-alpha) and Interleukin-1 beta (IL-1 beta) gene expression levels were assessed by RT-PCR analysis in liver tissues. In addition, liver tissues were evaluated histopathologically as a result of hematoxylin eosin (H&E) staining. Results: TNF-alpha and IL-1 beta gene expression levels in liver tissues were compared between groups and the results were statistically significant in all groups (p<0.000). Histopathological evaluation in H&E sections, sinusoidal dilatation, and hydropic degeneration were not observed in the liver tissue architecture of G1 (C). As a result of the examination of the sections belonging to G2 (AR), the location of hepatocyte cords and minimal destruction in portal areas were observed in organ architecture, and intensive sinusoidal dilatation was observed. Sinusoidal dilation was advanced in G3 (AR+SF). In addition, swelling and moderate hydropic degeneration were observed in hepatocytes, and focal lytic necrosis areas were not observed in G3 (AR+SF). All groups in terms of sinusoidal dilation and hydropic degeneration were significant differences statistically (p=0.000;0.001). Discussion: The present data indicated that oleuropein treatment suppresses inflammation in liver tissue caused by AR and it has no side effect in the experimental AR model. Oleuropein can be used as an auxiliary agent in the treatment of routine AR.Öğe The efficacy of Ankaferd Blood Stopper® in an experimental Asherman syndrome model created in rats(Galenos Yayincilik, 2019) Buyuk, Basak; Beyazit, FatmaObjective: Asherman syndrome (AS) is a progressive disease involving menstrual disorders, recurrent pregnancy losses, and infertility developing as a result of partial or full blockade of the uterine cavity with adhesions. AS generally develops after trauma to the basal layer of the endometrium. In spite of a variety of methods such as adhesiolysis, inserting intrauterine devices, and administering high doses of estrogen, treatments remain insufficient. This study aimed to assess the effects of local intrauterine Ankaferd Blood Stopper (ABS) administration in inducing endometrial proliferation and building a normal endometrial layer in a rat model. Materials and Methods: AS was induced in 30 female Wistar albino rats. The rats were randomized into three groups: Group 1: AS group Group 2: AS + serum physiologic (SP) group Group 3: AS + ABS group AS model was induced in all animals. The uterine horns were harvested after 15 days of therapy and investigated for inflammation, fibrosis, and immunohistochemical (IHC) markers. Results: Compared with the other groups, fibrosis, and inflammation were significantly reduced in group 3 (chi-square, p=19.000, 0.001 and 26.365, <0.001, respectively). The IHC assessment showed that the tumor necrosis factor-alpha receptor levels were not different (Kruskal-Wallis H=0.091, p=0.995), but the interleukin (IL)-1 beta and IL-6 expression was reduced significantly in group 3 (H, p=18.706, <0.001, and 22.114, <0.001, respectively). Conclusion: The therapeutic effects of local administration of ABS in rats with AS model were demonstrated histopathologically and immunohistochemically. Based on these results, ABS administration in addition to the current treatments for AS may increase the treatment success and reduce the need for advanced treatment.
