Yazar "Bayram, Abdurrahman" seçeneğine göre listele

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    Determination of Culturable Airborne Mold on Dusty Days in an Urban Atmosphere in the Eastern Mediterranean
    (Springer Nature, 2024) Palaz, Elif; Elbir, Tolga; Menteşe, Sibel; Bayram, Abdurrahman; Kara, Melik
    Recent studies have demonstrated that the amount of specific airborne mold types and their concentrations increase during dust events. This study investigates the effect of long-range dust transport on airborne mold composition between September 2020 and May 2021 in the urban atmosphere of Izmir, Turkey, a coastal city in the Eastern Mediterranean. A total of 136 airborne mold samples, 107 in non-dusty days and 29 in dusty days, were collected. Two different culture media, Potato Dextrose Agar (PDA) and Malt Extract Agar (MEA), were used to enumeration and genus-based identification of the airborne mold. Also, the effects of several meteorological parameters such as wind, air temperature and humidity, as well as particulate matter (PM10) concentrations, on the composition of airborne mold assemblages, were estimated seasonally. To determine the dusty and non-dusty days, several tools such as HYSPLIT Trajectory Model, NASA EOSDIS Worldview, WMO SDS-WAS North Africa-Middle East-Europe (NA-ME-E) and ECMWF CAMS Forecast Model were used. The total mold concentrations on PDA and MEA culture media ranged 49–1400 CFU/m3 and 28–1400 CFU/m3, respectively. Total mold concentrations that determined in PDA or MEA in spring and autumn were significantly higher than those occurred in winter, indicating that seasonal patterns of the meteorological parameters influenced the mold levels and genus prevalence in the region. Some of the mold genera, including Cladosporium sp., Chrysosporium sp., Aspergillus sp., Bipolaris sp., Alternaria sp. and yeasts, were more abundant during dusty days than non-dusty days. The correlation analysis showed a significant relation between airborne mold concentration, wind velocity and air temperature (p??0.05) was only significant in winter. © The Author(s), under exclusive license to Springer Nature Switzerland AG 2024.
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    Seasonal variability of airborne mold concentrations as related to dust in a coastal urban area in the Eastern Mediterranean
    (Springer Heidelberg, 2023) Palaz, Elif; Mentese, Sibel; Bayram, Abdurrahman; Kara, Melik; Elbir, Tolga
    Recent studies have demonstrated that the amount of specific airborne mold types and their concentrations increase during dust events. This study investigates the seasonal variation of airborne mold concentrations before, during, and after the dust transport in an eastern Mediterranean coastal area, Izmir city, Turkey. A total of 136 airborne mold samples were collected between September 2020 and May 2021. Two different culture media, namely Potato Dextrose Agar (PDA) and Malt-Extract Agar (MEA), were used for enumeration and genus-based identification of the airborne mold. In addition to culture media, the influences of air temperature, relative humidity, and particulate matter equal to or less than 10 mu m (PM10) were also investigated seasonally. The HYSPLIT trajectory model and web-based simulation results were mainly used to determine dusty days. The mean total mold concentrations (TMC) on dusty days (543 Colony Forming Unit (CFU)/m(3) on PDA and 668 CFU/m(3) on MEA) were approximately 2-2.5 times higher than those on non-dusty days (288 CFU/m(3) on PDA and 254 CFU/m(3) on MEA) for both culture media. TMC levels showed seasonal variations (p<0.001), indicating that meteorological parameters influenced mold concentrations and compositions. Some mold genera, including Cladosporium sp., Chrysosporium sp., Aspergillus sp., Bipolaris sp., Alternaria sp., and yeast, were found higher during dusty days than non-dusty days. Thus, dust event impacts levels and types of airborne molds and has implications for regions where long-range dust transport widely occurs.
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    The effect of different incubation conditions and culture media on airbone bacteria and fungi level
    (Pamukkale Univ, 2023) Palaz, Elif; Mentese, Sibel; Bayram, Abdurrahman; Kara, Melik; Elbir, Tolga
    The choice of culture media used for airborne bacteria and fungi sampling is the most critical factor that quantitatively affects the determination of bacteria and fungi. In this study, simultaneous bacterial and fungal samples were collected in the air of Izmir city center using eight different media types. Bacterial samples were collected on Columbia blood agar (CBA), Chocolate agar (Choc), Tryptone Glucose Yeast Extract Agar (TGEA), Reasoners 2A agar (R2A) and Plate Count Agar (PCA) media, and these samples were conditioned at two different incubation temperatures and periods. The growth of bacteria was followed at 37 C-degrees for 2 days and at 50 C-degrees between 2 and 15 days. Malt-Extract Agar (MEA), Dichloran Rose Bengal Chloramphenicol (DRBC) and Potato Dextrose Agar (PDA) media were used for fungi samples. All fungal samples were incubated at 25 C-degrees for 3-7 days. At the end of the incubation period, the number of colonies and total bacteria and fungi concentrations was determined. The incubation temperature was determined to affect the total bacteria concentrations in other media except for PCA (p>0.05). Cladosporium sp was the most frequently observed genus in fungal samples collected on three media. In terms of colony numbers, the media were sorted as PDA>MEA>DRBC. Among the fungi, the number of colonies of Chrysosporium sp. formed in the MEA medium was approximately ten times higher than in PDA and DRBC. The order of the maximum number of isolated fungi in the media used for the samples collected simultaneously is MEA>DRBC>PDA. This research is the first study to determine the concentrations of stable bacteria and fungi that can withstand extreme conditions (50 C-degrees) under different incubation conditions, as it will guide future airborne microorganism sampling in urban areas with the variety of media and incubation conditions it has examined. In addition, this study is also important because the World Health Organization has invited countries to fight against the threat of Antimicrobial Resistance in recent years.