Yazar "Aydeger, Cemre" seçeneğine göre listele
Listeleniyor 1 - 4 / 4
Sayfa Başına Sonuç
Sıralama seçenekleri
Öğe A Novel Approach for Arteriovenous Fistula Maturation; Effects of Melatonin Loaded PLGA Nanofibers in Rats(Galenos Publ House, 2025) Aykora, Damla; Sahin, Serpil; Aydeger, Cemre; Yulek, Ozden; Alkan, Sevil; Oral, Ayhan; Jajere, Muhammad UmarAim: Arteriovenous fistula (AVF) is a cannulation method that is accessed by a peripheric vein and an artery. AVF provides vascular access for chronic kidney disease patients so they can receive hemodialysis. AVF could be created by surgical intervention and facilitates arterial to venous circulation for rapid recovery. However, AVF maturation depends on venous proliferation and luminal diameter which allows the optimum flow rate for continuing circiulation and hemodialysis. Due to multiple unexpected conditions, non-maturation of AVFs limits the efficacy of the hemodialysis so patients must receive another surgery for AVF cannulation. Materials and Methods: In this study, we aimed to utilize the effects of melatonin (MT), which is known to have antioxidant, anti-inflammatory, and antiapoptotic effects, to provide longer and more effective use of AVFs via a novel technique. For this purpose, firstly by electrospinning method, polylactic-co-glycolic acid (PLGA) nanofiber membranes were developed. After MT is loaded into the PLGA and characterized. Biodegradation and drug release profiles were analyzed. An in vivo study was performed in Wistar Albino male rats (n=18). Rats were randomly divided into three experimental groups; Sham, PLGA, and MT/PLGA respectively (n=6). AVF model was established in all groups between arteria carotica and vena jugularis under general anesthesia. The Sham group did not receive any treatment or biomaterial application. The developed membranes were placed onto the AVFs in PLGA and MT/PLGA groups. All rats were sacrificed on the 28th of the experiment. The anastomosis sites of all animals were harvested for histopathological analysis. Results: Our results showed MT/PLGA group indicated increased maturation levels conmpared to Sham group (p<0.05). Conclusion: The results showed that PLGA/MEL may be a promising material for early AVF maturation.Öğe Effects of hyperbaric oxygen treatment on liver and kidney tissue in chronic arsenic toxicity(Undersea & Hyperbaric Medical Soc Inc, 2022) Eroglu, Huseyin Avni; Buyuk, Basak; Oztopuz, Ozlem; Makav, Mustafa; Aydeger, CemreArsenic (As) is a toxic substance that damages the human body through exposure to drinking water. This exposure damages many organs and tissues in the body, especially the liver and kidneys. Hyperbaric oxygen (HBO2) therapy is a treatment method that acts by reducing oxidative stress parameters in tissues with high-pressure oxygen. Based on this, our study aimed to investigate the effectiveness of HBO2 on liver and kidney tissues with chronic arsenic toxicity. In the study 24 male Wistar albino rats (220-300 g, two to three months old) were equally divided into four groups: Control; As; HBO2; and As+ HBO2. All animals were housed in individual cages. The toxicity model was created by adding arsenic to drinking water at a dose of 5 mg/kg/ day for 60 days. HBO2 was applied 2 ATA pressure for 90 minutes a day for five days. At the end of the study, liver and kidney tissues were taken and stored for analysis. In liver tissue, histopathological showed that arsenic reduced inflammatory cell infiltration, sinusoidal congestion, and hydropic degeneration, while HBO2 increased these measures. Similar results were found by TUNEL method. In kidney tissue, both histopathologic and TUNEL method examinations found similar results with the liver: The As group was more damaged than the As+ HBO2 group.Öğe The effects of branched chain amino acid supplement on kidney tissue of exercising rats(Asoc Regional Dialisis Trasplantes Renales, 2022) Aydeger, Cemre; Eroglu, Huseyin AvniIntroduction: One of the supplements used in exercise programs are branched-chain amino acids (BCAAs), which are preferred because of their effect on the regeneration of muscle protein synthesis. However, due to their properties, BCAAs increase their amount in the blood in a short time. In this case the result may increase the workload of the kidneys. Based on the information, this study investigated the effects of resistance exercise and BCAA supplements on kidney tissue. Methods: A total of 24 Wistar Albino male rats were equally divided into 4 groups: Control, BCAA, Exercise and Exercise + BCAA. In the six-week study, resistance swimming exercise was applied to the exercise groups. BCAA supplementation was given to BCAA groups at 2.5 mg/kg doses before exercise. At the end of the study, histological, immunochemical and RT-PCR analyzes were performed. Results: As a result of the findings, it was found that the use of BCAA supplements together with exercise caused tubular necrosis (p=0.002). There was a significant increase in caspase 3 IHC staining findings in BCAA and Exercise + BCAA groups compared to the control group (p=0.011; p=0.02). In addition, KIM-1 expression levels were higher in the Exercise group than in all other groups (p=0.004; p = 0.003; p=0.008). Conclusion: As a result, BCAA consumption with resistance exercise caused damage to kidney tissue.Öğe The Role of miR-330-3p in UV-induced Photokeratitis: A Pilot Experimental Study(Galenos Publ House, 2025) Eroglu, Huseyin Avni; Yavuz, Basak; Aydeger, Cemre; Erdogan, Hakika; Aykora, DamlaAim: Ultraviolet (UV) radiation-induced ocular diseases pose a growing challenge to public health. In recent years, miRNA-based therapeutics have gained attention in the treatment of ocular diseases. miR-330-3p has anticarcinogenic, anti-inflammatory, and anti-apoptotic effects. This study aimed to investigate the effects of miR-330-3p against photokeratitis following UV radiation. Materials and Methods: Eighteen Wistar albino male rats were randomly divided into three groups (n = 6) : control, UV, and UV+eye drop. Chronic exposure to UV radiation was conducted for 30 days, 2 hours a day. One mu l of miR-330-3p-based eye drops was applied to the UV+eye drop group once daily for 7 days. Following the treatments, eye tissues were harvested and evaluated microscopically. Results: There was no statistically significant difference between groups in inflammation, neovascularization, epithelial proliferation, and collagen density parameters. However, the edema levels in the UV group increased compared to the control and UV+eye drop groups (all p < 0.001 ) The collagen density, however, increased in the UV group and decreased in the UV+eye drop group, but the results did not indicate a significant difference (p > 0.05) Conclusion: miR-330-3p presents a promising treatment option for corneal damage arising from photokeratitis. Our study is the first to explore the alleviating effects of miR-330-3p in photokeratitis, yielding encouraging results.
