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    Evaluation of hepatic and remote organ injury in an experimental liver ischemia-reperfusion model in rats and the effects of quercetin on this damage
    (Turkish Assoc Trauma Emergency Surgery, 2025) Ari, Neziha Senem; Yulug, Esin; Ari, Bunyamin; Kaya, Cansu; Kose, Goksen Derya Reis; Zengin, Tugba; Keskin, Oguzhan
    BACKGROUND: This study aims to show the changes in the liver, lung, kidney, and heart in the liver ischemia-reper fusion model rats and the effect of quercetin on these changes histopathologically and immunohistochemically. METHODS: Eighteen Sprague Dawley rats were classified into three groups: Group 1 sham, Group 2 ischemia-reper fusion Group 3 ischemia-reper fusion + quercetin (IR+Q). For three days, distilled water was given to Group 1, and quercetin was given Group 3 via gavage. At the end of the third day, abdominal opening-closing was applied to Group 1, and 4 hours of reperfusion applied to Groups 2 and 3 after 1 hour of ischemia by clamping the hepatoduodenal ligament, and all rats were euthanized. Liver, kidney, and heart tissue samples were stained with Hematoxylin Eosin (HE), Masson Trichrome, Periodic Acid-Schiff (PAS), and TUNEL (Terminal deoxynucleotidyl transferase (TdT) deoxyuridine triphosphate nick end labeling assay) to assess apoptosis and examined histopathologically and immunohistochemically under a light microscope. RESULTS: In the liver, the damage score was significantly higher in the IR group than in the sham group, while it was significantly in the IR+Q group than in the IR group. While there was no significant difference between the groups in semi-quantitative scoring parameters, the Apoptotic Index was significantly higher in the IR group than in the sham group and significantly lower in the group than in the IR group. In the lung, no significant difference in lung damage scores between the groups was observed. While Apoptotic Index was significantly higher in the IR group than in the sham group, it was significantly lower in the IR+Q group than IR group. In the kidneys, tubular cell degeneration and intertubular vascular congestion were significantly higher in the IR group than the sham group. While the Apoptotic Index was higher in the IR group than in the sham and IR+Q groups, it was higher in the group than in the sham group. In the heart, there was no difference between the groups in terms of myocardial cell degeneration vascular damage. The apoptotic index was significantly higher in the IR group than in the sham and IR+Q groups. CONCLUSION: Our results indicate that histopathological damage occurs in the liver, lung, kidney, and heart in the experimentally created IR model, and quercetin application decreases IR-related damage and apoptosis in these organs.
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    Öğe
    Histological and flow cytometric evaluation of astaxanthin's effects against cyclophosphamide induced heart injury in rats
    (Taylor & Francis Ltd, 2025) Zengin, Tuğba; Tekelioğlu, Yavuz; Keskin, Oğuzhan; Reis Köse, Göksen Derya; Ari, Neziha Senem; Arıcı, Tuğba; Çetinavcı, Dilan
    In this study, the protective effect of astaxanthin (AST) against cyclophosphamide (CP) induced adult rat heart damage was investigated. Eighteen rats were divided into 3 groups as Group 1: control, Group 2: cyclophosphamide and Group 3: cyclophosphamide + astaxanthin. The CP group, received a 200 mg/kg single dose intraperitoneal (i.p.) injection of CP on the seventh day of the experiment, while the control group received no treatment. For CP+AST group 25 mg/kg/day AST administered by oral gavage on days 1-7 and on the 7th day 200 mg/kg/day CP was administered by i.p injection. On the 8th day, the rats were sacrificed by exsanguination and the hearts were dissected. Histopathological examinations were performed by Hematoxylin&Eosin (H&E), Masson Trichrome and Periodic Acid-Schiff (PAS) staining methods; Annexin-V and Anti-NOX2/gp91phox analyzes were performed by flow cytometry. In histological evaluation of the CP Group; disruptions in cardiac histology and increased PAS(+) staining were observed. These findings were reduced in the CP+AST group compared to the CP group. According to flow cytometry measurements, there was an increase in Annexin-V and Anti-NOX2/gp91phox bound cells in the CP group. With the AST pretreatment, in the CP+AST group Annexin-V and Anti-NOX2/gp91phox bound cell level showed decrease. Based on our study's data, CP may alter cardiac histology and have a negative impact on apoptosis and oxidative damage processes. Astaxanthin may ameliorate these effects of CP on the heart. To enhance the assessment of this protective effect, we propose conducting future research utilizing varied dosages, application durations and advanced analytical techniques.