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    Characterisation of Pea Milk Analogues Using Different Production Techniques
    (Faculty Food Technology Biotechnology, 2024) Andac, Ali Emre; Tuncel, Necati Baris; Tuncel, Nese Yilmaz
    Research background. Among legumes, peas are characterised by their high protein content, low glycaemic index and exceptional versatility. However, their potential as a food is often compromised by their undesirable off-flavour and taste. Hence, this study focuses on minimising off-flavours through simple pretreatments with the aim of improving the potential for the production of pea milk analogues. Pea milk analogues are a burgeoning type of plant-based milk alternatives in the growing plant-based market. Experimental approach. Pea seeds were subjected to different pretreatments: (i) i ) dry milling, (ii) ii ) blanching followed by soaking in alkaline solution and subsequent dehulling and (iii) iii ) vacuum. Typical physicochemical properties such as pH, viscosity, colour, titratable acidity and yield were measured to obtain a brief overview of the products. Consumer acceptance test, descriptive sensory analysis, gas chromatography-mass spectrometry and gas chromatography-olfactometry were used to map the complete sensory profile and appeal of the pea milk substitutes. Results and conclusions. The L* values of the pea milk analogues were significantly lower than those of cow's milk, while a* , b* , viscosity and pH were similar. In the descriptive sensory analysis, sweet, astringent, pea-like, cooked, hay-like, boiled corn and green notes received relatively higher scores. The vacuum-treated pea milk analogues received higher scores for flavour and overall acceptability in the consumer acceptance test. The pre- treatments resulted in significant changes in the volatile profiles of the pea milk analogues. Some volatiles typically associated with off-flavour, such as hexanal, were found in higher concentrations in blanched pea milk analogues. Among the applied pretreatments, vacuum proved to be the most effective method to reduce the content of volatile off-flavour compounds. Novelty and scientific contribution. This study stands out as a rare investigation to characterise pea milk analogues and to evaluate the impact of simple pretreatments on the improvement of their sensory properties. The results of this study could contribute to the development of milk alternatives that offer both high nutritional value and strong appeal to consumers.
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    Characterization and effects of heat treatments on antinutritional components and enzyme activities in sainfoin (Onobrychis viciifolia L.) seeds: A high-protein alternative plant-based food source
    (Elsevier, 2025) Andac, Ali Emre; Tuncer, Nese Yilmaz; Tulbek, Mehmet caglar; Tuncel, Necati Baris
    The aim of this study was to characterize and explore the food potential of sainfoin seeds, which are primarily used in their green plant form to feed livestock. A detailed assessment of the seed's proximate composition, enzyme activity levels, and antinutrient content was performed. The seeds were then subjected to infrared, hot air, and blanching treatments to examine how these processing methods affect enzyme activities and antinutrients. In addition, optimization of processing conditions was carried out using the response surface method and central composite design to ensure low antinutrient levels, minimal color change, and total enzyme inactivation. Sainfoin seeds have a crude protein content of 42 % in dry matter, making them a promising alternative plant-based protein source. They also contain 45 % dietary fiber, 9 % crude fat, and significant levels of potassium and magnesium. The unprocessed seeds exhibited 2.01 U/g lipase activity and 10.51 U/g lipoxygenase activity, which were inactivated with infrared treatment at 2000 W IR power in 75 s. In terms of antinutrients, sainfoin seeds contain 247 mg/100 g phytic acid, 2582 TIU/g trypsin inhibitor activity, 7.9 mg catechin equivalents of tannin /100 g, and 24.97 mg/100 g hydrocyanic acid. The optimal process conditions were 2000 W IR power and 105 s for IR treatment, 150 degrees C and 103 min for hot air treatment, and 81 degrees C and 16 min for blanching. The antinutrient levels and enzyme activities in unprocessed sainfoin seeds are comparable to those found in conventional legumes, although sainfoin seeds contain a higher amount of tannins. While infrared treatment was most effective for enzyme inactivation, the impact of the different processing methods varied across the parameters studied.
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    The impact of milling degree on physicochemical, nutritional, rheological, textural properties, and in vitro digestibility of rice: From brown rice to white rice
    (Elsevier Sci Ltd, 2025) Tuncel, Nese Yilmaz; Andac, Ali Emre; Kaya, Havva Polat; Tuncel, Necati Baris
    The objective of this study was to characterize the rice milling fractions acquired at each stage of a commercial milling system. This characterization included an analysis of color, ash content, dietary fiber, mineral composition, as well as antinutritional compounds like phytic acid, trypsin inhibitor activity, and saponin. Additionally, we investigated in vitro starch and in vitro protein digestibility, along with pasting, cooking, and textural properties. Our findings revealed that milling improved the visual appeal of rice (e.g., volume expansion, weight gain, whiteness) and notably enhanced its starch digestibility. However, milling reduced dietary fiber, mineral content, antinutrients, cooking time, and texture characteristics such as hardness, chewiness, gumminess, springiness, and cohesiveness to varying degrees. Certain parameters, such as dietary fiber, exhibited a gradual change with the duration of milling, while others, such as mineral content and texture, showed significant variation at the initial stage of milling, particularly at the first mill.