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    A global bibliometric analysis of Salmonella Typhi over the past 52 years (1970-2022)
    (J Infection Developing Countries, 2025) Altun, Serap Kilic; Aydemir, Mehmet Emin; Alkan, Sevil
    Introduction: Salmonella Typhi (S. Typhi), a bacterial human-restricted pathogen, is an essential systemic health problem of global importance in humans and animals. This study aimed to determine the overall scientific impact of Salmonella Typhi (S. Typhi) research using bibliometric methods. Methodology: In this research, a bibliometric analysis was performed on S. Typhi using the Web of Science (WoS) database and the Bibliometrix R package. A total of 1,966 articles, published between 1970-2022, were analyzed. Results: This bibliometric analysis showed that the most productive years in the process were 2012 and 2022. This analysis also showed that Infection and Immunity Journal was the leading journal with a total of 1,332 articles in publications related to S. Typhi research, with the USA being the most productive country in S. Typhi publications, as well as having the highest co-authorship collaboration. The University System of Maryland ranked first with 110 records when affiliations were analyzed. The United States Department of Health and Human Services was at the top of the Funding Agencies analysis. Analysis of the most cited authors revealed that Parry CM was the first most cited author (n = 90) of S. Typhi publications. Conclusions: This bibliometric analysis showed that the countries with high economic income were dominant in the studies of published articles, affiliations, and funding agencies on S. Typhi. Collaboration of researchers from countries with low or middle incomes with S. Typhi epidemics and researchers from countries with high economic income will make the fight against these bacteria more effective.
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    Detection of Mycobacterium avium subspecies paratuberculosis Survival in Retail Pasteurized and Raw Tank Milk Using Propidium Monoazide qPCR
    (Imr Press, 2025) Aydemir, Mehmet Emin; Altun, Serap Kilic; Yigin, Akin; Alkan, Sevil; Durmaz, Hisamettin
    Background: Mycobacterium avium subspecies paratuberculosis (MAP) is the causative agent of paratuberculosis, also known as Johne's disease, in ruminants and is associated with Crohn's disease in humans. Due to its resistance to pasteurization, MAP can be transmitted through contaminated milk and milk products, posing a food safety risk.Methods: This study aimed to detect and assess the viability of MAP in retail pasteurized and raw tank cow milk in & Scedil;anl & imath;urfa, Turkey, using the propidium monoazide (PMA)-quantitative polymerase chain reaction (qPCR) method. A total of 130 milk samples (50 pasteurized and 80 raw tank cow milk) were collected from local shops and dairies. Samples were tested for the presence of MAP, and viable bacteria were further quantified using PMA-qPCR.Results: MAP was not detected in any of the pasteurized milk samples. One (1.42%) raw milk sample tested positive for MAP, but further PMA-qPCR analysis indicated that the bacteria were not viable.Conclusions: The PMA-qPCR method can effectively determine the viability of MAP in milk. Raw bulk milk was found to be at risk of MAP contamination; thus, it is recommended that raw milk be consumed with caution, ensuring proper hygiene and storage, and ideally, should not be consumed raw due to potential public health risks.