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    A Simultaneous Analytical Method for Duplex Identification of Porcine and Horse in the Meat Products by EvaGreen based Real-time PCR
    (Korean Soc Food Science Animal Resources, 2015) Sakalar, Ergun; Ergun, Seyma Ozcirak; Akar, Emine
    A duplex real-time polymerase chain reaction (PCR) based assay for the detection of porcine and horse meat in sausages was designed by using EvaGreen fluorescent.dye. Primers were selected from mitochondrial 12S rRNA and 16S rRNA genes which are powerful regions for identification of horse and porcine meat. DNA from reference samples and industrial products was successfully extracted using the GI-DAGEN Multi-Fast DNA Isolation Kit. Genomes were identified based on their specific melting peaks (Mp) which are 82.5 degrees C and 78 degrees C for horse and porcine, respectively. The assay used in this study allowed the detection of as little as 0.0001% level of horse meat and 0.001% level of porcine meat in the experimental admixtures. These findings indicate that EvaGreen based duplex realtime PCR is a potentially sensitive, reliable, rapid and accurate assay for the detection of meat species adulterated with porcine and horse meats.
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    Application of carboxymethylcellulose hydrogel based silver nanocomposites on cotton fabrics for antibacterial property
    (Elsevier Sci Ltd, 2015) Bozaci, Ebru; Akar, Emine; Ozdogan, Esen; Demir, Asli; Altinisik, Aylin; Seki, Yoldas
    In this study, fumaric acid (FA) crosslinked carboxymethylcellulose (CMC) hydrogel (CMCF) based silver nanocomposites were coated on cotton fabric for antibacterial property for the first time. The performance of the nanocomposite treated cotton fabric was tested for different mixing times of hydrogel solution, padding times and concentrations of silver. The cotton fabrics treated with CMC hydrogel based silver nanocomposites demonstrated 99.9% reduction for both Staphylococcus aureus (Sa) and Klebsiella pneumonia (Kp). After one cycle washing processes of treated cotton fabric, there is no significant variation observed in antibacterial activity. From SEM and AFM analyses, silver particles in nano-size, homogenously distributed, were observed. The treated samples were also evaluated by tensile strength, thermogravimetric analysis (TGA), Fourier transform infrared spectroscopy (FTIR) analysis, fluid absorbency properties, and whiteness index. The treatment of cotton fabric with CMCF hydrogel did not affect the whiteness considerably, but increased the absorbency values of cotton. (C) 2015 Elsevier Ltd. All rights reserved.
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    Development of a traceable molecular hygiene control method (TMHCM) for human DNA content in foods
    (Elsevier Sci Ltd, 2017) Sakalar, Ergun; Ergun, Seyma Ozcirak; Pala, Cigdem; Akar, Emine; Atasoglu, Cengiz
    The aim of this study was to develop a molecular technique to determine the level of human originated DNA contamination in unhygienic food products. In the study, four model foods were prepared under both hygienic (H) and non-hygienic (NH) conditions and the human originated microbial loads of these products were determined. DNA was extracted from the model foods and human buccal samples by GIDAGEN Multi-fast DNA isolation kit. A primer specific region of human mitochondrial D-Loop was designed. The level of human DNA contamination in the model foods was determined by real-time PCR. The sensitivity of the technique developed here was 0.00001 ng DNA/PCR. In addition, the applicability of the traceable molecular hygiene control method (TMHCM) was tested in 60 food samples from the market. The results of this study demonstrate that DNA based TMHCM can be used to predict to what extent foods meet the human oriented hygienic conditions. (C) 2016 Published by Elsevier Ltd.
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    Improvement of the electromechanical performance of carboxymethylcellulose-based actuators by graphene nanoplatelet loading
    (Springer, 2015) Ozdemir, Okan; Karakuzu, Ramazan; Sarikanat, Mehmet; Seki, Yoldas; Akar, Emine; Cetin, Levent; Yilmaz, Ozgun Cem
    In this article, the effects of graphene loading (0.1, 0.2, 0.3 wt%) on both the electromechanical and mechanical properties of carboxymethylcellulose (CMC)-based actuators were investigated. CMC-based graphene-loaded actuators were prepared by using 1-butyl-3-methylimidazolium bromide. The synthesized graphene-loaded actuators were characterized by Fourier transform infrared, X-ray diffraction analysis, thermogravimetric analysis, scanning electron microscopy, and tensile tests. Electromechanical properties of the actuators were obtained under DC excitation voltages of 1, 3, 5, and 7 V with a laser displacement sensor. According to the obtained results, the ultimate tensile strength of CMC-based actuators containing 0.3 wt% graphene was higher than that of unloaded actuators by approximately 72.8 %. In addition, the Young's modulus value of the graphene-loaded actuators increased continuously with increasing graphene content. Under a DC excitation voltage of 5 V, the maximum tip displacement of 0.2 wt% graphene-loaded actuators increased by about 15 % compared to unloaded actuators.