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Öğe Analysis of High Molecular Weight Glutenin Subunit Composition of Pure Lines Selected from Eastern Turkey Bread Landraces(Ankara Univ, Fac Agr, 2015) Yildiz, Ayse; Akan, Kadir; Akcura, MevlutIn this research, high molecular weight glutenin (HMWG) subunit composition, found on Glu-1 locus of 41 genotypes selected according to grain yield, yield components and some quality properties from bread wheat landraces collected from Eastern Turkey, has been investigated by using SDS-PAGE method. Of the bread wheat pure lines, nine Glu-1 locus alleles have been determined in genotypes. While only null allele (c) on Glu-A1 locus has been determined, on Glu-B1 locus 7+8 subunit (b) in 35 pure lines (85.4%), 13+16 subunit (f) in 2 pure lines (4.9%), subunits 7 (a), 14+15 (h), 17+18 (i) and 6+8 (d) (2.4%) have been determined in only one genotype. On Glu-D1 locus allele a in 34 pure lines (82.9%) and allele d in 7 pure lines (17.1%) have been determined. In this research in which 7 different HMWG subunit combination were determined, the most common group found in 32 pure lines (78.0%) was n, 7+8 and 2+12. Of the genotypes, only 5 pure lines (12.2%) have taken 8 quality score value whereas the quality score value of 36 pure lines (87.8%) has been determined changing between 4 and 6.Öğe Assessment of the reactions of pure lines selected from Turkish bread wheat landraces against bunt disease (Tilletia foetida) with the GGE-biplot method(Cambridge Univ Press, 2018) Akcura, Mevlut; Akan, KadirThe present research was conducted to determine the reactions of 200 pure lines selected from bread wheat landraces collected from 18 provinces and seven regions of Turkey against bunt disease (Tilletia foetida) under field conditions for 3 years. Bunt disease reactions of pure lines were assessed based on the infected spike/total spike ratio. For visually assessed materials, the GGE-biplot method, where G = genotype effect and GE = genotype-by-environment effect, was used to group the reactions against bunt disease. Fifty-nine pure lines showed high resistance (with infection rates ranging from 0.1 to 10%); 24 in the moderate resistance (with infection rates ranging from 10.1 to 25%); 75 in the moderate susceptibility (with infection rates ranging from 25.1 to 45%); 38 in the susceptibility (with infection rates ranging from 45.1 to 70%) and finally four in the highly susceptibility (with infection rates of >70.1%). PC1 and PC2 of the GGE-biplot graph created over the years explained 76.49% of the total variation. The GGE-biplot graph provided efficient identification of resistant genotypes. The lowest PC1 values and PC2 values close to 0.0 explained the resistance of pure line to bunt disease best. The resistance of pure lines to bunt disease over the biplot decreased from the first section through the last section. Based on the results of present study, 19 pure lines (located within the first circle of the biplot graph) were selected for resistance breeding programmes against the diseases.Öğe BIPLOT ANALYSIS OF LEAF RUST RESISTANCE IN PURE LINES SELECTED FROM EASTERN ANATOLIAN BREAD WHEAT LANDRACES OF TURKEY(Soc Field Crop Sci, 2017) Akcura, Mevlut; Akan, Kadir; Hocaoglu, OnurThe present research was conducted to determine the reactions of 42 pure lines selected from bread wheat landraces of Eastern Anatolia, Turkey, against the leaf rust (Puccinia triticina) disease under field conditions across 7 environments. G (Genotype), GE (Genotype Environment) biplot analysis method was used to determine the reactions of landraces against leaf rust disease. GGE-biplot graph created to assess leaf rust disease was explained a 78.12% of total variation. While E3 and E2 constituted the first and second mega environments respectively, the other four environments constituted the third and fourth mega environments. The lowest PC1 values and PC2 values close to 0.0 explaining the resistance of pure lines to leaf rust at best in the biplot. Reactions of landraces varied based on their distance from the Average Environment Axis (AEA). While the pure lines with the same or similar reactions in 7 experimental environments fell close to the axis, ones with different reactions in one or more environments were relatively distant. The pure lines of EA15 and EA19 were identified as the most resistant and stable genotypes in all environments when EA42 and EA41 were the most susceptible/stable genotypes in all environments. Pure lines that were resistant or moderately resistant at all seven tested environments should be useful for breeding wheat cultivars with resistance to leaf rust in Turkey.Öğe Evaluating Scald Reactions of Some Turkish Barley (Hordeum vulgare L.) Varieties Using GGE Biplot Analysis(Mdpi, 2023) Akan, Kadir; Cat, Ahmet; Hocaoglu, Onur; Tekin, MehmetScald caused by the fungal pathogen Rhynchosporium commune is a significant foliar disease affecting barley production on a global scale, and it leads to substantial reductions in both yield and quality of barley. In the current study, the reactions of 40 Turkish barley (Hordeum vulgare L.) varieties to scald were evaluated under natural conditions in canakkale and Kirsehir in 2021-2022, and Antalya and Siirt locations in 2022-2023 growing seasons. Field trials were conducted according to randomized block design with three replications in each year; the spore concentration was 1 x 10(6) spores per mL, and it was applied to the varieties three times at different growth stages. The reactions of barley varieties were assessed using a newly designed two-digit scale ranging from 11 to 99. Based on their scale values, the varieties were categorized as immune (0), resistant (11-35), moderately resistant (36-55), moderately susceptible (56-75), and susceptible (76-99). In addition, genotype plus genotype-by-environment (GGE) interactions of scale values were analyzed through GGE Biplot and explained 97.65% of the total variation. The ranking of genotypes based on scale groups generally showed consistency with GGE Biplot results, but GGE Biplot offered a more detailed classification, especially for moderately susceptible varieties. The relationship between the two methods indicated the relative stability of variety reactions, as GGE Biplot analysis also considered genotype stability. In conclusion, the use of the newly developed scale for evaluating scald reactions in barley gives reliable results. In addition, identified resistant varieties can serve as valuable genetic resources for further breeding studies.Öğe Sakız Fasulyesinin (Cyamopsis tetragonoloba L.Taub.) Tane Verimi ve Sakız Özellikleri Yönünden Türkiye'nin Farklı Bölgelerine Adaptasyonunun Araştırılması(2020) Akçura, Mevlüt; Akan, Kadir; Atmaca, Evren; Kara, Burhan; Yılmaz, Mehmet Fatih; Kaplan, Mahmut; Kökten, KağanProje ile ülkemizin farklı çevre koşullarında tane verimi ve sakız içeriği yönünden uygun olan sakız fasulyesi genotiplerinin geliştirilmesi, sakız fasulyesi için en uygun çevrelerin tespit edilmesi, elde edilen sakızın teknolojik özelliklerinin belirlenmesi ve sakız alındıktan sonra kalan posanın yem özelliklerinin incelenmesi hedeflenmiştir. Materyal olarak Hindistan ve Pakistan?dan temin edilen popülasyonlar içerisinden Çanakkale sulu koşullarında 2011?2015 yılları arasında teksel seleksiyon ile seçilen saf hatlardan olumlu özellikleri (Çanakkale koşullarına tane verimi, olgunlaşma süresi, hastalıklara dayanıklılık vb. yönünden uyum sağlayan) taşıyan 86 adet hat ile Hindistan?da tescil ettirilmiş 4 çeşit kullanılmıştır. Projenin birinci yılında, Çanakkale (2 set), Bandırma (2 set), Burhaniye (2 set), İzmir (Bayındır), Kahramanmaraş (Merkez), ve Bingöl (Merkez) lokasyonlarında standart çeşitler ile hatları toplam 9 çevrede 41 özellik ( tane verimi, tohum, fenolojik, sakız ve yem) yönünden karşılaştırmak amacıyla dikdörtgen latis (9 x 10) deneme desenine göre 3 tekerrürlü denemeler kurulmuştur. Bu faaliyetlerin sonucuna göre tane ve sakız verimi ile sakız özellikleri yönünden en yüksek değerlere sahip olan 25 adet hat belirlenmiştir İkinci ve üçüncü yıllarda ise belirlenen 25 adet hat ve 4 standart çeşit ile aynı lokasyonlara ilave olarak Isparta (Merkez) lokasyonunda tesadüf blokları deneme desenine göre üç tekerrürlü olarak toplam 14 adet deneme (2 yıl 7 lokasyon) kurulmuştur. Denemelerde tane verimi, tohum, fenolojik, sakız ve yem özelliklerinden oluşan toplam 41 adet özellik incelenmiştir. Elde edilen sonuçların değerlendirilmesinde farklı stabilite parametreleri ile GGE-biplot yöntemi kullanılmıştır. Bu yöntemler ile deneme kurulan çevreler arasında yıllara değişmekle birlikte en uygun çevrelerin sırasıyla, Kahramanmaraş, İzmir, Çanakkale ve Burhaniye çevrelerinin olduğu, Bingöl ve Isparta çevrelerinin ise uygun olmadığı, hatlar arasında ise en iyilerin 23, 12, 13 ve 16 nolu hatların olduğu belirlenmiştir. En iyi olan hatlardan bir tanesinin ülkemizin ilk sakız fasulyesi çeşitleri olarak tescillenmesi için Tohumluk Tescil ve Sertifikasyon Merkezi?ne müracaatı için hazırlıklar devam etmektedir.Öğe Türkiye'nin değişik illerinden toplanmış yerel kışlık ekmeklik buğday çeşitlerinden seçilen saf hatların kalite özellikleri ve bazı önemli fungal hastalıklara karşı reaksiyonlarının belirlenmesi(2014) Akçura, Mevlüt; Kökten, Kağan; Göçmen, Aysun Akcaçık; Aydoğan, Seydi; Akan, Kadir; Kılıç, Hasan; Mert, Zafer[Abstract Not Available]Öğe Türkiye'nin Doğu Bölgesinden Toplanan Bazı Yerel Ekmeklik Buğday Popülasyonlarından Seçilen Saf Hatların Yüksek Molekül Ağırlıklı Glutenin Alt Birimlerinin İncelenmesi(2015) Yıldız, Ayşe; Akan, Kadir; Akçura, MevlütBu araştırmada Türkiyenin doğu bölgesinden toplanan bazı yerel ekmeklik buğday popülasyonlarından seçilen saf hatlardan tane verimi, verim unsurları ve bazı kalite özelliklerine göre belirlenen 41 adet genotipin, SDS-PAGE yöntemi kullanılarak Glu-1 lokusunda bulunan yüksek molekül ağırlıklı glutenin (YMAG) alt birimleri incelenmiştir. Ekmeklik buğday saf hatlarında Glu-1 lokusuna ait 9 tane allel belirlenmiştir. Glu-A1 lokusuna ait sadece null alleli (c) belirlenirken, Glu-B1 lokusuna ait 7+8 alt birimi (b) 35 (% 85.4) saf hatta 13+16 alt birimi (f) 2 (% 4.9) saf hatta, 7 (a), 14+15 (h), 17+18 (i) ve 6+8 (d) alt birimleri ise sadece 1er (% 2.4) saf hatta gözlenmiştir. Glu-D1 lokusunda a alleli 34 (% 82.9) saf hatta, d alleli 7 (% 17.1) saf hatta belirlenmiştir. Yedi farklı YMAG alt birimleri kombinasyonlarının saptandığı çalışmada en yaygın olarak (% 78.0) gözlenen n, 7+8 ve 2+12 kombinasyonu 32 saf hatta belirlenmiştir. Sadece 5 saf hat (% 12.2) 8 kalite skor değerini alırken, 36 (% 87.8) saf hattın skor değerinin 4-6 arasında değiştiği tespit edilmiştir. Anahtar Kelimeler: Türkiye yerel ekmeklik buğdayları; SDS-PAGE; Yüksek molekül ağırlıklı glutenin (YMAG)
