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    Investigation of Antioxidant Properties and Bioactive Composition of Allium tuncelianum ((Kollman) Ozhatay, Matthew & Siraneci) and Allium sativum L.
    (2018) Şehitoğlu, Müşerref Hilal; Karakan, Faika Yaralı; Kızılkaya, Bayram; Öztopuz, Rahime Özlem; Gülçin, İlhami
    In the present study, the extracts of Allium tuncelianum and Allium sativum L. were evaluatedfor antioxidant capacities, fatty acid, amino acid and phenolic compositions. In order to evaluate the antioxidantcapacity of the extracts, total antioxidant amount, radical scavenging and chelating activities, reducing powerby cupric reducing antioxidant capacity and ferric reduction antioxidant power methods were performed andcompared with reference antioxidants. Quantitative amounts of phenolic compounds were determined by theliquid chromatography–mass spectrometry. Gas chromatography–mass spectrometry and high performance liquidchromatography methods were used for the fat / fatty acid and amino acid compositions, respectively. The contentof p-Coumaric acid in Tunceli garlic was found higher compared to the cultuvated garlic. Total antioxidant capacityIC50 values were determined in Tunceli garlic as 72.20 µg ml-1; in cultuvated garlic as 63.80 µg ml-1. In termsof fatty acid compositions, Tunceli garlic (?-3, 3.92%; ?-6, 46.91%; ?-9, 14.16%) was observed having moreeffective level of essential omega acids compared to the cultuvated garlic (?-3, 3.20%; ?-6, 50.37%; ?-9, 5.18%).According to essential amino acid contents, the Tunceli garlic was found to be richer than cultuvated garlic. As aresult, due to the high biological activity of Tunceli garlic, it would be more beneficial in diet, in terms of cancerand cardiovascular disease treatment.
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    Öğe
    Protective effect of metformin against lithium-induced cerebral neurotoxicity in rats
    (2021) Malçok, Ümit Ali; Şehitoğlu, Müşerref Hilal; Buyuk, Basak; Taş, Halil İbrahim
    Neurotoxic effects are seen in patients using lithium regardless of dose. The neuroprotective activity of metformin used in the treatment of patients with type 2 diabetes mellitus is known. In this study, the protective efficacy of metformin against the neurotoxic effects of lithium was investigated. Neurotoxicity was induced by administering high dose oral lithium (40 mmol/kg) to rats for two weeks. Four groups were formed, with 6 rats in each group. OSI indices were calculated by measuring serum TAS and TOS levels. TAS, TOS, OSI, Gpx ADAM10, and ADAM17 levels were measured in the brain tissue. The neuroprotective activity of metformin was examined histopathologically by calculating the pyknosis scores, vacuolization, and interstitial edema in the hippocampus and cortex tissues. When lithium and metformin were given together, serum levels of TAS, TOS, and OSI were lower than that of the Lithium group (p<0.001), whereas the TAS level in the brain tissue was higher than that of the lithium group (p<0.05). With the concomitant administration of lithium and metformin, ADAM10 levels decreased (p<0.05), whereas ADAM17 and Gpx levels increased (p>0.05). In histopathological evaluation, pyknosis, interstitial edema, and vacuolization scores of the hippocampus and cortex regions increased with lithium alone but were low when lithium was given together with metformin. Levels of the antioxidant molecules Gpx and TAS decreased with lithium, and ADAM10 and ADAM17 proteins, which indicate the strength of intercellular bonding, increased by administering metformin. In conclusion, the toxic effect of lithium on brain tissue was reduced by metformin.
  • Küçük Resim
    Öğe
    Thymol regulates the Endothelin-1 at gene expression and protein synthesis levels in septic rats
    (Elsevier Ireland Ltd, 2023) Şehitoğlu, Müşerref Hilal; Öztopuz, Rahime Özlem; Kılınç, Namık; Ovalı, Mehmet Akif; Büyük, Başak; Gülçin, İlhami
    Sepsis is a serious systemic inflammatory response to infections. In this study, effects of thymol treatments on sepsis response were investigated. A total of 24 rats were randomly divided into 3 different treatment groups, namely as Control, Sepsis and Thymol. A sepsis model was created with a cecal ligation and perforation (CLP) in the sepsis group. For the treatment group, 100 mg/kg dose of thymol was administered via oral gavage and sepsis was established with a CLP after 1 h. All rats were sacrificed at 12 h post-opia. Blood and tissue samples were taken. ALT, AST, urea, creatinine and LDH were evaluated to assess the sepsis response in separated sera. Gene expression analysis was conducted for ET-1, TNF-alpha, IL-1 in lung, kidney and liver tissue samples. ET-1 and thymol interactions were determined by molecular docking studies. The ET-1, SOD, GSH-Px and MDA levels were determined by ELISA method. Genetic, biochemical and histopathological results were evaluated statistically. The pro-inflammatory cytokines and ET-1 gene expression revealed a significant decrease in the treatment groups, while there was an increase in septic groups. SOD, GSH-Px and MDA levels of rat tissues were significantly different in the thymol groups as compared to the sepsis groups (p < 0.05). Likewise, ET-1 levels were significantly reduced in the thymol groups. In terms of serum parameters, present findings were consistent with the literature. It was concluded based on present findings that thymol therapy may reduce sepsis-related morbidity, which would be beneficial in the early phase of the sepsis.