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    EFFECTS OF DIFFERENT DOSES OF SHIITAKE MUSHROOM (LENTINUS EDODES) ON SPERM PARAMETERS AND TESTICULAR TISSUE IN RATS: AN EXPERIMENTAL STUDY
    (Pakistan Agricultural Scientists Forum, 2023) Büyük, Başak; Demir, Neslihan; Eroğlu, Hüseyin Avni; Öztopuz, Rahime Özlem
    Lentinus edodes has been cultivated and used as food and medicine in far eastern countries.The present study aimed to reveal whether L.edodes that is used for different purposes has a toxic effect on the male genital system and to investigate its relationship with male infertility by showing its effects on sperm parameters. Twenty-four male Wistar rats were randomly divided into three groups as Control (C), Low-dose (LD) and High-dose (HD). Rats in Control were administered saline physiological once a day for 7 days. The LD and HD groups received 100 and 400mg/kg of L.edodes extract, respectively, once daily for 7 days. At the end of 7 days of administration of treatments, semen analysis, micronucleus analysis, gene expression levels and testicular Johnsen scoring was performed. Comparisons between groups without normal distribution were analyzed using Kruskal-Wallis variance analysis and those between groups with normal distribution were analyzed using one-way analysis of variance, followed by Tukey's test. P values of <0.05 were considered statistically significant. It is demonstrated that both low and high doses of extract significantly reduced spermatogenesis in seminiferous tubules found in the testicles of rats. In addition, high-dose administration resulted in decreased total sperm counts, whereas the progressive and non-progressive sperm motility counts were significantly decreased in both experimental groups. The gene expression levels of Casp-3 and TNF-alpha were increased in both the LD and HD groups compared with that in the C. Oral administration of low and high doses of L.edodes led to reduced spermatogenesis in the testicles, tissue damage to the testicle and decreased motile and total sperm counts.
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    Investigation of Antioxidant Properties and Bioactive Composition of Allium tuncelianum ((Kollman) Ozhatay, Matthew & Siraneci) and Allium sativum L.
    (2018) Şehitoğlu, Müşerref Hilal; Karakan, Faika Yaralı; Kızılkaya, Bayram; Öztopuz, Rahime Özlem; Gülçin, İlhami
    In the present study, the extracts of Allium tuncelianum and Allium sativum L. were evaluatedfor antioxidant capacities, fatty acid, amino acid and phenolic compositions. In order to evaluate the antioxidantcapacity of the extracts, total antioxidant amount, radical scavenging and chelating activities, reducing powerby cupric reducing antioxidant capacity and ferric reduction antioxidant power methods were performed andcompared with reference antioxidants. Quantitative amounts of phenolic compounds were determined by theliquid chromatography–mass spectrometry. Gas chromatography–mass spectrometry and high performance liquidchromatography methods were used for the fat / fatty acid and amino acid compositions, respectively. The contentof p-Coumaric acid in Tunceli garlic was found higher compared to the cultuvated garlic. Total antioxidant capacityIC50 values were determined in Tunceli garlic as 72.20 µg ml-1; in cultuvated garlic as 63.80 µg ml-1. In termsof fatty acid compositions, Tunceli garlic (?-3, 3.92%; ?-6, 46.91%; ?-9, 14.16%) was observed having moreeffective level of essential omega acids compared to the cultuvated garlic (?-3, 3.20%; ?-6, 50.37%; ?-9, 5.18%).According to essential amino acid contents, the Tunceli garlic was found to be richer than cultuvated garlic. As aresult, due to the high biological activity of Tunceli garlic, it would be more beneficial in diet, in terms of cancerand cardiovascular disease treatment.
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    Neuroprotective Effects of Piceatannol on Olfactory Bulb Injury after Subarachnoid Hemorrhage
    (Springer, 2023) Akar, Ali; Öztopuz, Rahime Özlem; Büyük, Başak; Ovalı, Mehmet Akif; Aykora, Damla; Malçok, Ümit Ali
    Subarachnoid hemorrhage (SAH) accounts for 5% of all stroke cases and is responsible for significant permanent brain and neurological damage within the first few days. Loss of smell is one of those neurological disorders following olfactory bulb injury after SAH. Olfaction plays a critical role in several aspects of life. The primary underlying mechanism of olfactory bulb (OB) injury and loss of smell after SAH remains unknown. Piceatannol (PIC), a natural stilbene, possesses anti-inflammatory and anti-apoptotic effects against various diseases. In this study, we aimed to investigate the potential therapeutic effects of PIC on OB injury following SAH at molecular mechanism based on SIRT1, inflammatory (TNF-α, IL1-β, NF-κB, IL–6, TLR4), and apoptosis (p53, Bax, Bcl-2, caspase-3)-related gene expression markers and histopathology level; 27 male Wistar Albino rats were used in a pre-chiasmatic subarachnoid hemorrhage model. Animals were divided into groups (n = 9): SHAM, SAH, and PIC. Garcia’s neurological examination, brain water content, RT-PCR, histopathology, and TUNEL analyses were performed in all experimental groups with OB samples. Our results indicated that PIC administration significantly suppressed inflammatory molecules (TNF-α, IL–6, IL1-β, TLR4, NF-κB, SIRT1) and apoptotic molecules (caspase-3, p53, Bax). We also evaluated edema levels and cell damage in OB injury after SAH. Ameliorative effects of PIC are also observed at the histopathology level. Garcia’s neurological score test performed a neurological assessment. This study is the first to demonstrate the neuroprotective effects of PIC on OB injury after SAH. It suggests that PIC would be a potential therapeutic agent for alleviating OB injury after SAH.
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    Thymol regulates the Endothelin-1 at gene expression and protein synthesis levels in septic rats
    (Elsevier Ireland Ltd, 2023) Şehitoğlu, Müşerref Hilal; Öztopuz, Rahime Özlem; Kılınç, Namık; Ovalı, Mehmet Akif; Büyük, Başak; Gülçin, İlhami
    Sepsis is a serious systemic inflammatory response to infections. In this study, effects of thymol treatments on sepsis response were investigated. A total of 24 rats were randomly divided into 3 different treatment groups, namely as Control, Sepsis and Thymol. A sepsis model was created with a cecal ligation and perforation (CLP) in the sepsis group. For the treatment group, 100 mg/kg dose of thymol was administered via oral gavage and sepsis was established with a CLP after 1 h. All rats were sacrificed at 12 h post-opia. Blood and tissue samples were taken. ALT, AST, urea, creatinine and LDH were evaluated to assess the sepsis response in separated sera. Gene expression analysis was conducted for ET-1, TNF-alpha, IL-1 in lung, kidney and liver tissue samples. ET-1 and thymol interactions were determined by molecular docking studies. The ET-1, SOD, GSH-Px and MDA levels were determined by ELISA method. Genetic, biochemical and histopathological results were evaluated statistically. The pro-inflammatory cytokines and ET-1 gene expression revealed a significant decrease in the treatment groups, while there was an increase in septic groups. SOD, GSH-Px and MDA levels of rat tissues were significantly different in the thymol groups as compared to the sepsis groups (p < 0.05). Likewise, ET-1 levels were significantly reduced in the thymol groups. In terms of serum parameters, present findings were consistent with the literature. It was concluded based on present findings that thymol therapy may reduce sepsis-related morbidity, which would be beneficial in the early phase of the sepsis.