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Öğe Adipoz Kaynaklı Kök Hücreler ve Uygulama Alanları(2015) Kıvanç, Müjde; Öztürk, Şamil; Gökalp, Sevtap; Özdemir, İlhan; Tuğlu, İbrahimKompleks bir canlı olan insan, olağanüstü bir koruma mekanizması geliştirerek zararlı olabilecek etkenlerden mümkün olduğunca kendini uzak tutar ve bunu başarabilmek için de bazı farklılaşmamış hücreleri kullanır. Yani her türlü doku kayıplarında yeniden fonksiyon gösterebilmesinin sırrı sahip olduğu kök hücrelerde saklıdır. Vücudumuzda farklılaşmamış bir hücre olarak bulunan kök hücreler herhangi bir organa yerleştirildiklerinde farklılaşıp o dokunun özelliklerini kazanarak şimdiye kadar bilinen oldukça fazla dokunun yerine geçebilmekte ve bu sayede hastalıkları iyileştirip, dokuları yenileyebilmektedir. Kök hücre kaynağı olarak kullanılan, kordon kanı, kemik iliği ve yirmi yaş dişinden sonra bilim adamları insan yağ dokusunda da kök hücre araştırdılar ve yağ dokusunda diğer kaynaklardan çok daha fazla kök hücre bulunduğunu keşfettiler. Bu hücreler üzerinde yapılan in vitro çalışmalar şunu gösteriyor ki bu hücrelerden birçok değişkene bağlı istenen soya yönelik doğrudan kök hücre optimizasyonu yapılabilmektedir. Bu derlemede de, adipoz kaynaklı kök hücrelerin saflaştırılması ve uygulamalarda kullanılması ile ilgili mevcut literatür gözden geçirilerek bilim dünyasına katı sağlaması amaçlanmıştır.Öğe Adverse Effects of Ruditapes decussatus (Linnaeus, 1758) Diet on Stomach Tissues in Rats(2021) İrkin, Latife Ceyda; Öztürk, ŞamilRuditapes decussatus (Linnaeus, 1758) belongs to the Veneridae family is given as nutrients to rats. R. decussatus was collected from three different locations from the Çanakkale Strait. Twenty-four male Wistar Albino rats (270-310 g) were used in the study. There were six rats for each experimental group. The first group fed with standard rat feed, the second group fed with 80% R. decussatus + 20% standard rat feed daily; the third group fed with 80% R. decussatus + 20% standard rat feed every other day, the fourth group fed with 80% R. decussatus + 80% standard rat feed every three days. After routine histopathological follow-up, gastric tissue samples of all subjects were stained and examined under the light microscope. There were no histopathological findings in the gastric tissues of rats in the control group of hematoxylin-eosin staining. In gastric tissues of rats fed with R. decussatus, chronic gastritis caused by mononuclear inflammation between lamina propria and occasional gastric glands was noted. It was observed that inflammation severity and distribution were high especially in tissues of rats fed with R. decussatus every day. It was observed that the most commonly consumed shellfish may cause pathological picture on the digestive system of rats. As a result, increasing environmental pollution threatens the life of water as well as land life, and the consumption of living organisms exposed to polluted environments continues to threaten and affect human life. It is important to pay attention to the conditions under which the consumed products are collected and how they are collected.Öğe Effect of Mediterranean Mussels (Mytilus galloprovincialis) From Polluted Areas on Hepatotoicity in Rats by Immunuhistochemical Method(2021) İrkin, Latife Ceyda; Çakına, Suat; Özdemir, İlhan; Öztürk, ŞamilMussels (Mytilus galloprovincialis) are aquatic organisms that can accumulate all the factors that pollute the fresh andsaltwater environment. Although the Dardanelles has been exposed to environmental pollution for many years, it is also animportant region for crustaceans. We aimed to investigate the histopathological changes in the liver parenchyma which is animportant gland of the digestive system by feeding these mussels to rats. Twenty-four male Wistar albino were used in thestudy. The first group (control): fed with standard rat feed, the second group (experiment 1): 4/5 mussel + 1/5 standard ratfeed daily; third group (experiment 2): 4/5 mussel + 1/5 standard rat feed every other day, the fourth group (experiment 3):groups were formed with 4/5 mussel + 1/5 standard rat feed every three days. All liver tissue samples taken from theexperimental and control groups were stained with hematoxylin-eosin, and immuohitochemically staining inflammatorymarker TNF-? and NF-?B after routine histopathological follow-up and analyzed with a light microscope image analysissystem. It was observed that mononuclear cells caused inflammation of portal areas, increased sinusoidal dilatation andcongestion and degeneration due to vacuolization in hepatocytes in the liver parenchyma of mussel-fed rats. Besides,immunohistochemical staining, TNF-?, and NF-?B immunoreactivity were observed in the liver cells of especially in thesecond group of rats. As a result, it has been shown that the consumption of mussels obtained and marketed withoutconsidering environmental pollution may trigger important digestive system organs of liver diseases.Öğe Effects of Curcumin and Doxorubicin on the Viability of Neuroblastoma Cancer Cell Line(2024) Ünlü, İlker; Özdemir, İlhan; Öztürk, Şamil; Tuncer, Mehmet CudiAim: Neuroblastoma has a very important place among childhood diseases, and despite all the methods used in treatment, it is very difficult to prevent neuroblastoma invasion. The number of studies showing that curcumin, the most active component of turmeric, is not toxic, is increasing day by day. In this study, the anticancer activities of curcumin (Cur), one of the important active compounds, were demonstrated in the Neuroblastoma cancer cell line (Na2B). Method: Neuroblastoma cell line was used in the study. To determine the IC50 doses of Dox and Cur, Na2B cells line were cultivated with an automatic pipette. MTT analysis was performed to analyze cell survival (viability). Inhibition levels in the cells were determined at 24 and 48 hours. Results: While the IC50 of Na2B cell proliferation was approximately 124.5 uM at the 48th hour in Dox-treated cells, the IC50 value of Cur at the 48th hour was found to be 224.6 uM. Conclusion: These results showed that Cur could be an alternative agent in the treatment of neuroblastoma, and its fewer side effects compared to other chemotherapeutic agents such as Dox would increase its preferability.Öğe Evaluation of Regeneration and Apoptosis in Experimental Hepatic Resection of Rats(2021) Öztürk, Şamil; İrkin, Latife CeydaObjective: Apoptosis and accompanying regeneration begins immediately after liver resection and transpalantation. It is known that DNA synthesis reaches its maximum level in the first 24-48 hours after resection. This study was carried out to examine the regeneration and apoptotic process in the liver of rats that underwent 70% liver resection on days 3 and 7. Materials and Methods: 21 adult male Wistar Albino rats were used. The laparotomy with an upper midline incision was performed on the subjects for liver resection. Three groups were formed as the control (Group I), the group that was sacrificed 3 days after resection (Group II), and the group that was sacrificed 7 days after resection (Group III). Results: Sinusoidal dilatation and vacuolization in hepatocytes were observed in the liver tissues of all experimental groups except the control group. However, these histopathological findings were observed more in group II than other groups. It was observed that the apoptotic index and proliferation index values reached the maximum level in the group II. In group III, it was observed that these index values gradually decreased. Values of these parameters were found to be quite low in control group. Conclusion: As a result, comparisons of the mechanisms accompanying the regeneration occurring after resection were determined by immunohistochemical and TUNEL methods, and findings that shed light on clinical applications were obtained day by day.Öğe Experimental 70% Hepatectomy Model: Apoptotic Index, Proliferative Index and Mitotic Index(2021) Öztürk, Şamil; İrkin, Latife CeydaLiver regeneration is known to begin after experimentally induced liver resection. At first 24- 48 hours, DNA synthesis reaches the maximum level after resection and significantly regeneration known to occur on the first 10 days. In this study, we were examined the regeneration of liver on the 1st and 14th days after the resection. In experiment 21 Wistar albino male rats were used. The rats were randomly divided into three groups. The first group was the control, the second group was sacrificed one day after hepatectomy, and the third group was sacrificed 14 days after hepatectomy. All experimentally groups realized midline incision with laparotomy for resection of liver. Pedicles of the left lateral and median lobes of the liver were applied 70% hepatectomy by 4/0 silk binding. At the end of 1st and 14th days, liver tissue removed for light misroscobic analysis. No histopathological findings were found in the control group. The tissue of all experimentally groups were showed some histopatological changes such as sinuzoidal dilatation, vacuolization in the hepatocytes. This histopatological findings were seem to increase at grup II more than group III and group I with routin H&E staining. Mitotic index, apoptotic index and proliferation index of values at group II was reached the maximum level. Group III dramatically reduced the value of these index and were seem to reach near to values of the control group. Relative liver weight that determined each of experimentally groups were statistically significant differences compared to the control group. As a result, in this study, liver regeneration was shown in liver transplants without any agent that would contribute to the clinic.Öğe Gallik Asidin Rahim Ağzı Kanseri Hücre Hattına (HeLa) Karşı Antikanser Etkisi(2023) Sarı, Umut; Afşin, Yasemin; Özdemir, İlhan; Özçelik, Serap Mutlu; Öztürk, ŞamilAmaç: Servikal kanser, kadın infertilitesinde oldukça önemli yer teşkil eden ve dünya çapında kadınları en çok etkileyen dördüncü kanser olarak karşımıza çıkmaktadır. Doxorubicin (Dox), yumurtalık ve meme kanseri tedavisinde en sık reçete edilen kemoterapötiklerdendir. Kemoterapötik ilaçlar çok etkili tedavi sonuçları ile yanıt oluştursalar da, ciddi yan etkileri oluşturmakta ve ilaç direnci gelişmesi bu ilaçların kullanımına kısıtlama getirmektedir. Antikanser aktiviteye sahip doğal ürünlerin kullanılması bu sorunların kısmen üstesinden gelinmesine yardımcı olabilir. Gallik asit (GA), çeşitli bitki ve gıdalarda yaygın olarak bulunur ve çeşitli biyolojik etkilere sahip olduğu bildirilmiştir. Bu çalışmada GA'in ve güçlü bir kemoterapi ajanı olan Dox’in HeLa hücreleri ve insan deri keratinosit hücre hattı (HaCaT) üzerindeki hücre büyümesinin inhibisyonu ve ölümü ile ilgili etkileri MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) yöntemi ile belirlenmesi amaçlanmıştır. Yöntemler: Çalışmada serviks adenokarsinoma hücre serisi HeLa (CCL-2™) ve sağlıklı hücre serisi olarak insan deri keratinosit hücre serisi HaCat (RRID: CVCL_0038) kullanılmıştır. Dox ve GA’in IC50 (inhibitory concentration) dozlarının belirlenmesi için HeLa ve HaCaT hücre hattı otomatik multipipet yardımı ile ekimi yapılmıştır. Daha sonra hücre sağkalım (viyabilite) analizini yapmak için MTT testi uygulanmıştır. Bulgular: HeLa hücre büyümesi, Dox ile muamele edilen hücrelerde 48. saatte yaklaşık IC50 137.6 uM iken, GA'in 48. saatteki IC50 değeri 239.2 uM bulunmuştur. Sonuç: Bu sonuçlar, GA’in Dox’nun etkisini güçlendirdiğini ve Dox/GA asit kombinasyonunun, rahim ağzı kanseri tedavisinde diğer kemoterapötik kombinasyonları için daha düşük yan etkilerle umut verici bir alternatif olabileceğini göstermektedir.Öğe Histopathological Changes in The Spinal Cord Tissue of Rats Administered an Experimental Mussel Diet(2024) Ünlü, İlker; Özdemir, İlhan; Tuncer, Mehmet Cudi; Öztürk, ŞamilAim: Regional eating habits show that it causes neurodegenerative problems due to heavy metals that can accumulate in consumed foods and affect tissues such as the nervous system. Since crustaceans such as mussels feed by filtering the water, they are exposed to toxic plankton and various chemicals, especially heavy metals. Due to the limitations of experimental studies on this subject, the effects of mussel consumption on the spinal cord were investigated. Methods: In this study, histopathological changes in the spinal cord tissue of rats fed with shellfish collected from the Dardanelles were determined. The subjects were divided into two groups, and the first group was fed standard rat food for 4 weeks, and the second group was fed a mussel diet. At the end of the study, spinal cord tissue samples taken from rats were subjected to routine histopathological procedures and evaluated under a light microscope. Results: In the experimental group, a decrease in the number of neurons in the medulla spinalis and an increase in the number of astrocytes were noted. TUNEL staining showed that apoptosis occurred intensively in glial cells, but did not occur in anterior and posterior horn motor neurons. Conclusion: The findings showed that long-term mussel consumption can cause axonal damage in motor and sensory neurons and degeneration in glial cells. For this reason, it is important for health that marine diets in coastal areas are made with healthy and hygienic products.Öğe Histopathological Evaluation of Muscle Tissue of Horse Mackerel (Trachurus trachurus, Linnaeus, 1758) in Çanakkale Strait(2022) İrkin, Latife Ceyda; Öztürk, Şamil; Aldık, RuhayIt is supported by studies that heavy metals and other water pollutants can reach humans through the food chain because of accumulation in organs of digestion, respiration, and muscles of fish, and may cause toxic effects depending on the amount of pollution and accumulation. This study was carried out by obtaining Horse mackerel samples from the Çanakkale Strait, which are fish species with high economic and nutritional value, as well as frequently consumed, seasonally (spring, summer, autumn, and winter). In the study, the tissues dissected from the dorsolateral muscles of the fresh fish were taken into Bouin's fixative, and sections were taken after routine histological follow-up. The findings revealed degenerative and inflammatory findings in the muscle tissues of fish caught especially in the autumn season. In addition, a statistically significant difference was found in autumn samples in terms of immunoreactivity (p<0.05).Öğe Investigation of Regeneration Capacity in Rat Liver After Hepatic Resection(2021) Öztürk, Şamil; İrkin, Latife Ceyda; Özdemir, İlhanThe liver is the largest gland of the body that secretes both endocrine and exocrine secretions and plays a major role in the regulation of metabolic functions. Numerous factors such as drugs, chemicals, accidents, alcohol, surgical procedures can cause damage to the liver tissue. In this study, we aimed to determine the regeneration capacity of liver tissue in order to recover the mass loss after hepatic resection. In our experiment 21 Wistar albino male rats were used. All experimental groups applied midline incision with laparotomy for resection of liver. At the end of 1 and 7th days, liver tissue removed for light microscopic analysis. The rats were divided three groups: Control, group 1: hepatectomy one day after liver resection, group 2: hepatectomy seven days after liver resection. The tissue of all experimental groups were showed some histopatological changes such as sinuzoidal dilatation, vacuolization in the hepatocytes. These histopathological differentiation was found to be severe in group II compared to other groups. At the end of the 7th day, it was observed that the regeneration increased significantly, and the mitotic index value reached almost the maximum level in the second group. It was observed that the mitotic index value gradually decreased in group I and reached values close to the control groupÖğe Investigation of Stem Cell Applications on In Vitro Fertilization in Rats(HELLENIC VETERINARY MEDICAL SOC., 2021) Gümürdü, A.; Öztürk, Şamil; Aydemir, I.; Tuğlu, M. İ.We aimed to search the effects of bone marrow-derived mesenchymal stem cell-conditioned media on in vitro fertilization by investigation of lifetime of germ cells cleavage, degeneration rates and embryo quality. For this purpose, firstly MSCs were isolated from femurs and tibias of the rat, and cells were cultured until the fourth passage. Sperm and oocytes were collected from male and female rats. Oocytes were added in Human Tubal Fluid Media (HTFM), Single Step Media (SSM), Alpha-MEM Media (AMM) and Bone Marrow-Derived Mesenchymal Stem Cell-Conditioned Media (CM). Thousand sperm were added into the media which including oocytes. Embryos were allowed to produce by IVF. The development of the embryos was followed until the 11th day, and the arrest, degeneration rates and alive embryos were established. The embryos reached 2, 4, 8, 16 cells stages and morula stage in the CM. While AMM had a negative effect on fertilization and embryo development, the most favourable effect was shown to be caused by CM in comparison with the other medias. These results have shown that the beneficial effects of CM in IVF would be a significant increase in the rate of fertility and development of embryosÖğe SPERMATOGENESİS AND DYNAMİC STEM CELLS(Rating Academy, 2018) Öztürk, Şamil; Çakına, Suat; Yeşil, Hayrunnisa; Tuğlu, M. İbrahimStem cell shave a tremendous therapeutic potential with their ability to continually renew themselves and their ability to transform into almost all mature cells in our body. In the male reproductive system, a dynamic group of stem cells proliferates and prevents in fertility. This process is called spermatogenesis in mammals and the testis occurs in seminiferous tubules. Testicular stem cells, called germ stem cells (GKH) are capable of rapid regeneration after self-renewal and injury during adult life. How did spermatocytes occur dynamically in the later stages of old age? which is an indispensable element of the male reproductive system and is necessary for the continuation of the next generation. There has always been a question I asked and the answer was sought. In our review study, the changes of spermatogonium proliferating with mitosis in seminiferous tubules up to spermatozoa with meiosis and stem cell reserves are discussedÖğe Sıçanlarda Trikloroasetik Asit ile İndüklenen Deneysel İntrauterin Adezyonlarda Kök Hücre Tedavisi(2022) Öztürk, Şamil; Otcu, Serap Mutlu Özçelik; Özdemir, İlhan; İrkin, Latife CeydaKemik iliğinden elde edilen kök hücrelerin, sıçanlarda kimyasal kullanılarak oluşturulan intrauterin adezyon modellemesinde rahim içi yapışıklıkların giderilmesinde ve blastosistin endometriuma implantasyonunun yeniden sağlanmasında etkinliği ve rolü araştırıldı. Deney modeli, trikloroasetik asit kullanılarak tek uterin hornda meydana gelen hasara karşı, yalnızca kültür besiyeri (CM), kök hücre ve 48 saatlik Niş kullanılarak farklı grup ve iki alt grup oluşturulmuştur. Çalışmada toplam 30 dişi ve 3 erkek rat kullanıldı. Akut fazda model oluşumundan hemen sonra tedaviye başlandı ve 10 gün sonra denekler gebe kalmaları için erkek sıçanlarla aynı kafese yerleştirildi. Daha sonra gebelik durumu ve doğan yavru sayısı değerlendirildi. Histolojik değerlendirme hematoksilen-eozin boyaması ile yapıldı. Yenidoğan sayısına göre hem histolojik hem de morfolojik değerlendirmelerde kök hücre uygulanan gruplarda diğer gruplara göre istatistiksel olarak anlamlı fark (p [EN] The effectiveness and role of stem cells obtained from bone marrow in removing the adhesion formed in intrauterine adhesion modeling using chemicals in rats and restoring the implantation of the blastocyst to the endometrium were investigated. The experimental model was created in a single horn using trichloroacetic acid. Three different groups and two subgroups were formed as only culture medium (CM), stem cell and 48-hour medium (Niche). A total of 30 female and 3 male rats were used in the study. Treatment was started immediately after model formation in the acute phase, and 10 days later, the subjects were placed in the same cage with male rats for conception. Then, the pregnancy status and the number of puppies born were evaluated. Histological evaluation was performed with hematoxylin-eosin staining.A statistically significant difference (pÖğe The Importance of Cited-1 and HIF-1? Immune Activity of Granulosa Cells in IVF Treatment(2024) Sarı, Umut; Özdemir, İlhan; Baş, Dilek Doğan; Öztürk, ŞamilAim: The aim of this study was to investigate the Cited-1 and HIF-1? immune activity in granulosa cells in follicular development in patients who underwent IVF for infertility. Materials and Methods: This study was conducted on 40 patients who were admitted to the assisted reproductive program with the complaint of infertility at the Gazi Yaşargil Training and Research Hospital Obstetrics and Gynecology Clinic IVF center between January 2022 and November 2022 and had primary or secondary infertility while starting the Ovum-Pick-Up (OPU) procedure. The fluid containing the granulosa cells was centrifuged at 3000 rpm for 10 min. The samples were fixed and processed for routine paraffine wax tissue embedding protocol. Sections were taken from paraffin blocks and immune stained with Cited-1 and HIF-1?. The preparations were examined under the microscope. Results: HIF-1? expression was positive in membrane of granulosa cells. The nuclei were apoptotic and pyknotic. Cited1 expression was positive in membrane of granulosa cells. the cells were pyknotic. Conclusion: The high level of HIF-1? immunopositivity and negative Cited1 immunoreactivity in the immunohistochemical staining after the granulosa cells around the oocytes collected from female patients admitted to the IVF clinic and diagnosed with infertility showed that granulosa cell viability may be important on oocyte quality.
