Yazar "Özdemir, İlhan" seçeneğine göre listele

Listeleniyor 1 - 8 / 8
  • [ X ]
    Öğe
    Adipoz Kaynaklı Kök Hücreler ve Uygulama Alanları
    (2015) Kıvanç, Müjde; Öztürk, Şamil; Gökalp, Sevtap; Özdemir, İlhan; Tuğlu, İbrahim
    Kompleks bir canlı olan insan, olağanüstü bir koruma mekanizması geliştirerek zararlı olabilecek etkenlerden mümkün olduğunca kendini uzak tutar ve bunu başarabilmek için de bazı farklılaşmamış hücreleri kullanır. Yani her türlü doku kayıplarında yeniden fonksiyon gösterebilmesinin sırrı sahip olduğu kök hücrelerde saklıdır. Vücudumuzda farklılaşmamış bir hücre olarak bulunan kök hücreler herhangi bir organa yerleştirildiklerinde farklılaşıp o dokunun özelliklerini kazanarak şimdiye kadar bilinen oldukça fazla dokunun yerine geçebilmekte ve bu sayede hastalıkları iyileştirip, dokuları yenileyebilmektedir. Kök hücre kaynağı olarak kullanılan, kordon kanı, kemik iliği ve yirmi yaş dişinden sonra bilim adamları insan yağ dokusunda da kök hücre araştırdılar ve yağ dokusunda diğer kaynaklardan çok daha fazla kök hücre bulunduğunu keşfettiler. Bu hücreler üzerinde yapılan in vitro çalışmalar şunu gösteriyor ki bu hücrelerden birçok değişkene bağlı istenen soya yönelik doğrudan kök hücre optimizasyonu yapılabilmektedir. Bu derlemede de, adipoz kaynaklı kök hücrelerin saflaştırılması ve uygulamalarda kullanılması ile ilgili mevcut literatür gözden geçirilerek bilim dünyasına katı sağlaması amaçlanmıştır.
  • [ X ]
    Öğe
    Effect of Mediterranean Mussels (Mytilus galloprovincialis) From Polluted Areas on Hepatotoicity in Rats by Immunuhistochemical Method
    (2021) İrkin, Latife Ceyda; Çakına, Suat; Özdemir, İlhan; Öztürk, Şamil
    Mussels (Mytilus galloprovincialis) are aquatic organisms that can accumulate all the factors that pollute the fresh andsaltwater environment. Although the Dardanelles has been exposed to environmental pollution for many years, it is also animportant region for crustaceans. We aimed to investigate the histopathological changes in the liver parenchyma which is animportant gland of the digestive system by feeding these mussels to rats. Twenty-four male Wistar albino were used in thestudy. The first group (control): fed with standard rat feed, the second group (experiment 1): 4/5 mussel + 1/5 standard ratfeed daily; third group (experiment 2): 4/5 mussel + 1/5 standard rat feed every other day, the fourth group (experiment 3):groups were formed with 4/5 mussel + 1/5 standard rat feed every three days. All liver tissue samples taken from theexperimental and control groups were stained with hematoxylin-eosin, and immuohitochemically staining inflammatorymarker TNF-? and NF-?B after routine histopathological follow-up and analyzed with a light microscope image analysissystem. It was observed that mononuclear cells caused inflammation of portal areas, increased sinusoidal dilatation andcongestion and degeneration due to vacuolization in hepatocytes in the liver parenchyma of mussel-fed rats. Besides,immunohistochemical staining, TNF-?, and NF-?B immunoreactivity were observed in the liver cells of especially in thesecond group of rats. As a result, it has been shown that the consumption of mussels obtained and marketed withoutconsidering environmental pollution may trigger important digestive system organs of liver diseases.
  • [ X ]
    Öğe
    Effects of Curcumin and Doxorubicin on the Viability of Neuroblastoma Cancer Cell Line
    (2024) Ünlü, İlker; Özdemir, İlhan; Öztürk, Şamil; Tuncer, Mehmet Cudi
    Aim: Neuroblastoma has a very important place among childhood diseases, and despite all the methods used in treatment, it is very difficult to prevent neuroblastoma invasion. The number of studies showing that curcumin, the most active component of turmeric, is not toxic, is increasing day by day. In this study, the anticancer activities of curcumin (Cur), one of the important active compounds, were demonstrated in the Neuroblastoma cancer cell line (Na2B). Method: Neuroblastoma cell line was used in the study. To determine the IC50 doses of Dox and Cur, Na2B cells line were cultivated with an automatic pipette. MTT analysis was performed to analyze cell survival (viability). Inhibition levels in the cells were determined at 24 and 48 hours. Results: While the IC50 of Na2B cell proliferation was approximately 124.5 uM at the 48th hour in Dox-treated cells, the IC50 value of Cur at the 48th hour was found to be 224.6 uM. Conclusion: These results showed that Cur could be an alternative agent in the treatment of neuroblastoma, and its fewer side effects compared to other chemotherapeutic agents such as Dox would increase its preferability.
  • [ X ]
    Öğe
    Gallik Asidin Rahim Ağzı Kanseri Hücre Hattına (HeLa) Karşı Antikanser Etkisi
    (2023) Sarı, Umut; Afşin, Yasemin; Özdemir, İlhan; Özçelik, Serap Mutlu; Öztürk, Şamil
    Amaç: Servikal kanser, kadın infertilitesinde oldukça önemli yer teşkil eden ve dünya çapında kadınları en çok etkileyen dördüncü kanser olarak karşımıza çıkmaktadır. Doxorubicin (Dox), yumurtalık ve meme kanseri tedavisinde en sık reçete edilen kemoterapötiklerdendir. Kemoterapötik ilaçlar çok etkili tedavi sonuçları ile yanıt oluştursalar da, ciddi yan etkileri oluşturmakta ve ilaç direnci gelişmesi bu ilaçların kullanımına kısıtlama getirmektedir. Antikanser aktiviteye sahip doğal ürünlerin kullanılması bu sorunların kısmen üstesinden gelinmesine yardımcı olabilir. Gallik asit (GA), çeşitli bitki ve gıdalarda yaygın olarak bulunur ve çeşitli biyolojik etkilere sahip olduğu bildirilmiştir. Bu çalışmada GA'in ve güçlü bir kemoterapi ajanı olan Dox’in HeLa hücreleri ve insan deri keratinosit hücre hattı (HaCaT) üzerindeki hücre büyümesinin inhibisyonu ve ölümü ile ilgili etkileri MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) yöntemi ile belirlenmesi amaçlanmıştır. Yöntemler: Çalışmada serviks adenokarsinoma hücre serisi HeLa (CCL-2™) ve sağlıklı hücre serisi olarak insan deri keratinosit hücre serisi HaCat (RRID: CVCL_0038) kullanılmıştır. Dox ve GA’in IC50 (inhibitory concentration) dozlarının belirlenmesi için HeLa ve HaCaT hücre hattı otomatik multipipet yardımı ile ekimi yapılmıştır. Daha sonra hücre sağkalım (viyabilite) analizini yapmak için MTT testi uygulanmıştır. Bulgular: HeLa hücre büyümesi, Dox ile muamele edilen hücrelerde 48. saatte yaklaşık IC50 137.6 uM iken, GA'in 48. saatteki IC50 değeri 239.2 uM bulunmuştur. Sonuç: Bu sonuçlar, GA’in Dox’nun etkisini güçlendirdiğini ve Dox/GA asit kombinasyonunun, rahim ağzı kanseri tedavisinde diğer kemoterapötik kombinasyonları için daha düşük yan etkilerle umut verici bir alternatif olabileceğini göstermektedir.
  • [ X ]
    Öğe
    Histopathological Changes in The Spinal Cord Tissue of Rats Administered an Experimental Mussel Diet
    (2024) Ünlü, İlker; Özdemir, İlhan; Tuncer, Mehmet Cudi; Öztürk, Şamil
    Aim: Regional eating habits show that it causes neurodegenerative problems due to heavy metals that can accumulate in consumed foods and affect tissues such as the nervous system. Since crustaceans such as mussels feed by filtering the water, they are exposed to toxic plankton and various chemicals, especially heavy metals. Due to the limitations of experimental studies on this subject, the effects of mussel consumption on the spinal cord were investigated. Methods: In this study, histopathological changes in the spinal cord tissue of rats fed with shellfish collected from the Dardanelles were determined. The subjects were divided into two groups, and the first group was fed standard rat food for 4 weeks, and the second group was fed a mussel diet. At the end of the study, spinal cord tissue samples taken from rats were subjected to routine histopathological procedures and evaluated under a light microscope. Results: In the experimental group, a decrease in the number of neurons in the medulla spinalis and an increase in the number of astrocytes were noted. TUNEL staining showed that apoptosis occurred intensively in glial cells, but did not occur in anterior and posterior horn motor neurons. Conclusion: The findings showed that long-term mussel consumption can cause axonal damage in motor and sensory neurons and degeneration in glial cells. For this reason, it is important for health that marine diets in coastal areas are made with healthy and hygienic products.
  • [ X ]
    Öğe
    Investigation of Regeneration Capacity in Rat Liver After Hepatic Resection
    (2021) Öztürk, Şamil; İrkin, Latife Ceyda; Özdemir, İlhan
    The liver is the largest gland of the body that secretes both endocrine and exocrine secretions and plays a major role in the regulation of metabolic functions. Numerous factors such as drugs, chemicals, accidents, alcohol, surgical procedures can cause damage to the liver tissue. In this study, we aimed to determine the regeneration capacity of liver tissue in order to recover the mass loss after hepatic resection. In our experiment 21 Wistar albino male rats were used. All experimental groups applied midline incision with laparotomy for resection of liver. At the end of 1 and 7th days, liver tissue removed for light microscopic analysis. The rats were divided three groups: Control, group 1: hepatectomy one day after liver resection, group 2: hepatectomy seven days after liver resection. The tissue of all experimental groups were showed some histopatological changes such as sinuzoidal dilatation, vacuolization in the hepatocytes. These histopathological differentiation was found to be severe in group II compared to other groups. At the end of the 7th day, it was observed that the regeneration increased significantly, and the mitotic index value reached almost the maximum level in the second group. It was observed that the mitotic index value gradually decreased in group I and reached values close to the control group
  • [ X ]
    Öğe
    Sıçanlarda Trikloroasetik Asit ile İndüklenen Deneysel İntrauterin Adezyonlarda Kök Hücre Tedavisi
    (2022) Öztürk, Şamil; Otcu, Serap Mutlu Özçelik; Özdemir, İlhan; İrkin, Latife Ceyda
    Kemik iliğinden elde edilen kök hücrelerin, sıçanlarda kimyasal kullanılarak oluşturulan intrauterin adezyon modellemesinde rahim içi yapışıklıkların giderilmesinde ve blastosistin endometriuma implantasyonunun yeniden sağlanmasında etkinliği ve rolü araştırıldı. Deney modeli, trikloroasetik asit kullanılarak tek uterin hornda meydana gelen hasara karşı, yalnızca kültür besiyeri (CM), kök hücre ve 48 saatlik Niş kullanılarak farklı grup ve iki alt grup oluşturulmuştur. Çalışmada toplam 30 dişi ve 3 erkek rat kullanıldı. Akut fazda model oluşumundan hemen sonra tedaviye başlandı ve 10 gün sonra denekler gebe kalmaları için erkek sıçanlarla aynı kafese yerleştirildi. Daha sonra gebelik durumu ve doğan yavru sayısı değerlendirildi. Histolojik değerlendirme hematoksilen-eozin boyaması ile yapıldı. Yenidoğan sayısına göre hem histolojik hem de morfolojik değerlendirmelerde kök hücre uygulanan gruplarda diğer gruplara göre istatistiksel olarak anlamlı fark (p [EN] The effectiveness and role of stem cells obtained from bone marrow in removing the adhesion formed in intrauterine adhesion modeling using chemicals in rats and restoring the implantation of the blastocyst to the endometrium were investigated. The experimental model was created in a single horn using trichloroacetic acid. Three different groups and two subgroups were formed as only culture medium (CM), stem cell and 48-hour medium (Niche). A total of 30 female and 3 male rats were used in the study. Treatment was started immediately after model formation in the acute phase, and 10 days later, the subjects were placed in the same cage with male rats for conception. Then, the pregnancy status and the number of puppies born were evaluated. Histological evaluation was performed with hematoxylin-eosin staining.A statistically significant difference (p
  • [ X ]
    Öğe
    The Importance of Cited-1 and HIF-1? Immune Activity of Granulosa Cells in IVF Treatment
    (2024) Sarı, Umut; Özdemir, İlhan; Baş, Dilek Doğan; Öztürk, Şamil
    Aim: The aim of this study was to investigate the Cited-1 and HIF-1? immune activity in granulosa cells in follicular development in patients who underwent IVF for infertility. Materials and Methods: This study was conducted on 40 patients who were admitted to the assisted reproductive program with the complaint of infertility at the Gazi Yaşargil Training and Research Hospital Obstetrics and Gynecology Clinic IVF center between January 2022 and November 2022 and had primary or secondary infertility while starting the Ovum-Pick-Up (OPU) procedure. The fluid containing the granulosa cells was centrifuged at 3000 rpm for 10 min. The samples were fixed and processed for routine paraffine wax tissue embedding protocol. Sections were taken from paraffin blocks and immune stained with Cited-1 and HIF-1?. The preparations were examined under the microscope. Results: HIF-1? expression was positive in membrane of granulosa cells. The nuclei were apoptotic and pyknotic. Cited1 expression was positive in membrane of granulosa cells. the cells were pyknotic. Conclusion: The high level of HIF-1? immunopositivity and negative Cited1 immunoreactivity in the immunohistochemical staining after the granulosa cells around the oocytes collected from female patients admitted to the IVF clinic and diagnosed with infertility showed that granulosa cell viability may be important on oocyte quality.