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    Evaluation of the Diagnostic Performance of a SARS-CoV-2 and Influenza A/B Combo Rapid Antigen Test in Respiratory Samples
    (MDPI, 2023) Dinç, Harika Öykü; Karabulut, Nuran; Alaçam, Sema; Kırkoyun Uysal, Hayriye; Daşdemir, Ferhat Osman; Önel, Mustafa; Çakan, Hüseyin
    This study aimed to evaluate the performance characteristics of a rapid antigen test developed to detect SARS-CoV-2 (COVID-19), influenza A virus (IAV), and influenza B virus (IBV) (flu) compared with those of the real-time reverse transcription-polymerase chain reaction (rRT-PCR) method. One hundred SARS-CoV-2, one hundred IAV, and twenty-four IBV patients whose diagnoses were confirmed by clinical and laboratory methods were included in the patient group. Seventy-six patients, who were negative for all respiratory tract viruses, were included as the control group. The Panbio™ COVID-19/Flu A&B Rapid Panel test kit was used in the assays. The sensitivity values of the kit were 97.5%, 97.9%, and 33.33% for SARS-CoV-2, IAV, and IBV, respectively, in samples with a viral load below 20 Ct values. The sensitivity values of the kit were 16.7%, 36.5%, and 11.11% for SARS-CoV-2, IAV, and IBV, respectively, in samples with a viral load above 20 Ct. The kit’s specificity was 100%. In conclusion, this kit demonstrated high sensitivity to SARS-CoV-2 and IAV for viral loads below 20 Ct values, but the sensitivity values were not compatible with PCR positivity for lower viral loads over 20 Ct values. Rapid antigen tests may be preferred as a routine screening tool in communal environments, especially in symptomatic individuals, when diagnosing SARS-CoV-2, IAV, and IBV with high caution.
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    Exploring the Effects of Lucilia sericata Larvae on Biofilm-forming Bacteria in Wounds
    (2022) Eğribel, Merve; Sirekbasan, Serhat; Çakan, Hüseyin; Polat, Erdal
    Objective: Biofilm-induced chronic wound infections are among the most significant causes of treatment failures. Healing chronic wounds with biofilm is a major issue worldwide due to high costs and adverse effects on patients. In this study, we aimed to investigate the impacts of Lucilia sericata larvae on biofilm-forming bacteria in chronic wounds. Method: We recruited 30 patients applying to Istanbul University-Cerrahpasa, Traditional and Complementary Medicine Research and Application Center, Wound Healing Unit, between December 2019 and March 2021. We obtained swab cultures before and immediately after applying Larval Debridement Therapy (LDT). After identifying isolated bacteria, we examined all agents by susceptibility and biofilm formation. Results: While isolating only a single bacterial genus/species in 19 patients, we could detect more than one bacterial genus/species in 11 of them. Moreover, we observed biofilm formation in bacteria isolated from 21 patients, but it was not the case in bacteria grown in 9 patients. Following LDT, we discovered that the biofilm layer on wounds was removed in a short time (two to three sessions). Conclusion: Although many methods are adopted in healing chronic wounds with biofilm formation, they often remain ineffective. The therapy with L. sericata larvae, a simple and effective method, may promote rapid healing of biofilm- forming wounds.
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    Investigation of gut microbiota in suicide cases instead of forensic sciences
    (2022) Ogdur, Murat; Çakan, Hüseyin; Evrensel, Alper
    Suicidal cases are criminal cases that either should be prevented or be investigated forensically after occurring. One of the aims of our study is that arrange the bacterial\rflora - one of the biological factors- in the gut to reduce suicide cases. In our study, we compared the bacterias which are based in the gut flora of suicidal death with the\rcontrol group among the 103 autopsy materials collected. In this study, besides the classic microbial and biochemical methods, we also used the Real-Time PCR and\rDroplet Digital PCR methods. According to the result of our study, it is determined that Bacteroides sp., Clostridium sp., Lactobacillus sp. and Bifidobacterium sp. which\raffect suicide are significantly different than control groups. It is thought that probiotic or prebiotic supplementation of individuals with intestinal flora imbalance may\rbe beneficial about reduce the suicidal cases which are caused by depression and anxiety. Therefore arranging of the gut flora may decrease the suicidal cases besides\rincreasing the academic studies shall have considerable importance and may gain favor in this sense.