A novel PEDOT-derived electroactive polymer-based enzymatic glucose biosensor enriched with gold nanoparticles

Abstract

In this study, 3,4-ethylenedioxythiophene derivative conjugated monomer 4-(dihexylamino)-9,12-bis(2,3-dihydrothieno[3,4-b][1,4]dioxin-5-yl)-7H-benzo [de]benzo[4,5]imidazo[2,1-a]isoquinolin-7-one (EDOTBN) was synthesized and electropolymerized on pencil graphite electrode (PGE) to fabricate a highly stable and sensitive sensor for glucose detection for the first time. The structurally high conjugation of the monomer and the effect of increasing the conductivity of the gold nanoparticles increased the covalent bonding ability of the enzyme to the modified electrode surface and provided an excellent immobilization. PGE/p(EDOTBN)/AuNPs/GOx designed biosensor was characterized by a wide linear glucose determination range between 1.169 x 10-2 and 10 mM with a limit of detection of 0.008461 mM and a sensitivity of 38.365 mu A/mMcm2. Furthermore, the prepared bioelectrode showed storage stability for 4 weeks. Recovery studies were also carried out to check the accuracy of the developed bioelectrode and the glucose concentration in commercial samples was determined accurately.