Investigation of hepatotoxic effect of bryophytes (Homalothecium sericeum (HEDW) Schimp.) on rat liver

Abstract

No evidence has been found to determine whether moss extract affects fibrotic, inflammatory, apoptotic and tissue homeostasis for tissue regeneration on rat's liver tissue. In our study, 1 ml of distilled water (Group I), and 50 mg/kg (Group II), 100 mg/kg (Group III), 300 mg/kg (Group IV), 500 mg/kg (Group V) for 30 days' doses of moss and by gavage of a total of 30 female rats in liver tissue due to the increase in the dose of moss histopathology was revealed. Also, immunohistochemical staining was performed to determine the immunoreactivity of transcription factor nuclear factor Kappa-B (NF-ΚB), tumor necrosis factor (TNF-α), which regulates inflammation and immune response, and TUNEL (Terminal deoxynucleotidyl transferase dUTP nick end labeling) for the assessment of the apoptotic process. The stained tissue samples were evaluated with the image analysis system under the research microscope and One-way ANOVA, Tukey test, which is one of the nonparametric tests, was used to determine the differences between the groups statistically and the results were evaluated according to p<0.05. We found that liver damage increased due to the increased dose of moss species used in the experiment. At the same time, antioxidant activity was increased, and apoptosis was increased. Considering these results, it was concluded that algae extract would have anticancer properties and could be used in the pharmaceutical industry.