Ahmadifar, EhsanShohreh, PoulinKalhor, NaserMoghadam, Mohsen ShahriariYılmaz, Sevdan2024-02-202024-02-202024Ahmadifar, E., Shohreh, P., Kalhor, N., Shahriari Moghadam, M., Yılmaz, S., Mohammadzadeh, S., … Abarghouei, S. (2024). Assessing the impact of dietary polystyrene nanoplastics on growth performance, immunological parameters, and antioxidant defense in zebrafish (Danio rerio). Journal of the World Aquaculture Society. https://doi.org/10.1111/jwas.130460893-88491749-7345https://doi.org/10.1111/jwas.13046https://hdl.handle.net/20.500.12428/5668This trial was performed in order to investigate the response of zebrafish (Danio rerio) to dietary exposure to polystyrene nanoplastics (PS-NPs) under laboratory conditions on fish growth and health. Healthy zebrafish (n=240) were divided into 12 tanks and fed with diets including 0 (T0), 100 (T1), 500 (T2), and 1000 (T3) mg kg−1 synthesized polystyrenes nanoplastics (PS-NPs) for 30 days. At the end of trial, fish fed the PS-NPs supplementation showed weight gain percentages of 79.45%, 70.35%, and 61.88% for T1, T2, and T3 groups, respectively, compared with 87.39% in the control fish. The SOD, GPX and MDA activities and cortisol levels increased by 47%, 32% and 35% and 46%, respectively, especially at high-dose administration (p < 0.05). The expression of GPX (T2-81% and T3-82%) and SOD (T2-101% and T3-187%) were remarkably upregulated in T2 and T3 groups. Moreover, the relative gene expression of HSP70, interleukin-1 (IL1), Interferon γ (IFN-γ) and tumor necrosis factor-α (TNF-α) increased by 178%, 202%, 154% and 307%, respectively, especially at high-dose administration (p < 0.05). The results of the present study demonstrated that exposure to PS-NPs especially at high concentrations (500 and 1000 mg kg−1 of diet) negatively influenced growth, health status-, antioxidant-, and immunity-related gene expression responses of zebrafish.eninfo:eu-repo/semantics/openAccessAttribution 3.0 United StatesAquatic pollutionNanoplasticPolystyreneZebrafishArticle10.1111/jwas.13046Q2WOS:0011372479000012-s2.0-85181486169