Mousavi, ShalalehMohammadzadeh, SedighehMood, Sara MehdizadehAhmadifar, EhsanSheikhzadeh, NajmehKalhor, NaserMoghadam, Mohsen Shahriari2025-01-272025-01-2720241642-34022300-8733https://doi.org/10.2478/aoas-2024-0043https://hdl.handle.net/20.500.12428/25205This trial investigated the efficacy of artichoke (Cynara scolymus) extract (AE) on the growth performance, immunity, antioxidant parameters, and resistance against Aeromonas hydrophila of goldfish (Carassius auratus). For this purpose, a total number of 470 gold fish with initial weight 5.70 +/- 0.2 g fed with four experimental diets including 0 (T0), 100 (T1), 150 (T2), and 200 (T3) mg kg v diet AE for 8 weeks. At the end of feeding trial, growth performances, serum immune parameters, and mucus antioxidant enzymes were measured. Fish were challenged with A. hydrophila, and the antioxidant and immunity-related gene expression were investigated. Based on the results, the highest final weight (FW) and weight gain (WG) attained in T2 and T3 (P<0.05). Immune factors including ACH50, lysozyme, and total immunoglobulin in T2 and T3 showed the highest values (P<0.05). The expression of GR, IL1 beta, TNF alpha, HSC70, HSP70, and HSP90 beta genes in T1, T2, and T3 were higher than the control (P<0.05). The GST expression was significantly enhanced in T2 (P<0.05). The present study demonstrated that the administration of AE, especially at doses of 150 mg kg(-1), could improve the growth, immunity, and antioxidant parameters, as well as enhance disease resistance against A. hydrophila in goldfish.eninfo:eu-repo/semantics/openAccessartichoke extractgoldfishimmunityantioxidative statusAeromonas hydrophilaArticle2441223123510.2478/aoas-2024-0043N/AWOS:0012074383000012-s2.0-85191784413Q1