Cetinkaya, AhmetKaya, S. IremUludağ Anıl, İnciKemal Sezgintürk, Mustafa KemalOzkan, Sibel A.2026-02-032026-02-03202597804432388409780443238833https://doi.org/10.1016/B978-0-443-23883-3.00007-6https://hdl.handle.net/20.500.12428/34315Hormones are biological entities that are released by endocrine glands or tissues, and the critical metabolic and physiological pathways of the body require various hormones. Blood levels of hormones in an individual are important indicators of healthy or abnormal conditions. Accurate, rapid, and sensitive determination of the concentrations of hormones, which are usually at the picomolar-nanomolar level, is critical in health applications. In addition to this, it may also be possible to aim to increase efficiency and promote growth by adding steroid hormones to foods inappropriately. Detection of hormone residues in foods is also important to prevent situations that threaten food safety and human health. Lateral flow assays (LFAs) are advantageous, paper-based analysis platforms in which separation and selectivity features are integrated. LFAs are advantageous, paper-based analysis platforms in which separation and selectivity features are integrated. LFA design includes parts such as the bioreceptor, control zone, etc., and detection methods such as colorimetry or fluorescence can be used. In this chapter, the applications of LFAs in the health field for purposes such as diagnostics or monitoring of a condition and their use in food safety are examined. After giving information about the design of a standard LFA and the assay procedure, current studies containing the most important applications in hormone determination were evaluated in terms of target hormone, assay type, label, detection method used in LFA, linear range, limit of detection, examined sample, and recovery% value. While the current status of LFAs on a critical issue, such as hormone analysis, is discussed in detail, the future course is also evaluated. © 2026 Elsevier Inc. All rights reserved..eninfo:eu-repo/semantics/closedAccessDeterminationFood safetyHealth applicationHormonesLateral flow assaysPoint-of-care analysisBook Chapter23726210.1016/B978-0-443-23883-3.00007-62-s2.0-105026834773N/A