Piskin, EnsarCetinkaya, AhmetEryaman, ZulalKaradurmus, LeylaUnal, Mehmet AltaySezgintürk, Mustafa KemalHizal, Julide2025-01-272025-01-2720240026-265X1095-9149https://doi.org/10.1016/j.microc.2024.111163https://hdl.handle.net/20.500.12428/24834Lactate detection is important for the food and healthcare industries, and it's especially important when there's tissue hypoxia, hepatic illness, bleeding, respiratory failure, or sepsis. A new molecularly imprinted polymer (MIP) based electrochemical sensor was fabricated for differential pulse voltammetric assay of L-lactate (LAC). By using ZIF-8@ZnQ nanoparticles, the number of regions and effective surface area were increased. The polymeric film was obtained using 4 aminobenzoic acid (4-ABA) as a functional monomer, ethylene glycol dimethacrylate (EGDMA) as a cross-linker, 2-hydroxyethyl methacrylate (HEMA) as basic monomers, and 2-hydroxy-2-methylpropiophenone one as initiator. The developed 4-ABA/LAC/ZIF-8@ZnQ@MIP-GCE was morphologically characterised using SEM and electrochemically using electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) measurements. A linear range of 0.1-1.0 pM LAC with a detection limit of 29.9 fM was found. Lastly, the MIP-based electrochemical sensor detected LAC in commercial human serum samples.eninfo:eu-repo/semantics/closedAccessMolecularly imprinted polymersL-lactateVoltammetryPhotopolymerizationNanoparticleArticle20410.1016/j.microc.2024.111163N/AWOS:0012719257000012-s2.0-85198249476Q1