Alan, CabirKocoglu, HasanAltintas, RamazanAlici, BulentErsay, Ahmet Resit2025-01-272025-01-2720111734-19221896-9151https://doi.org/10.5114/aoms.2011.22069https://hdl.handle.net/20.500.12428/23457Introduction: Transforming growth factor-beta 1 (TGF-beta 1) has a crucial role in collagen synthesis and fibrosis. TGF-beta 1 can be antagonized and/or reduced by the action of certain agents. We propose to identify the role of decorin in treatment of tubular and interstitial fibrosis and in the inhibition of TGF-beta 1 in an acute ischaemic kidney. Material and methods: We grouped 34 female Sprague Dawley type rats into 3 groups as 9 sham, 9 ischaemia-reperfusion (I/R) and 16 I/R + decorin respectively. The rats in the I/R + decorin group had decorin administered intraperitoneally at the dose of 0.1 mg/kg for 9 days after reperfusion. After 9 days, all the rats in the 3 groups were unilaterally nephrectomized. The TGF-beta 1 level was measured immunohistochemically in the nephrectomized material. Results: The TGF-beta 1 level was lower in the I/R + decorin group. Evaluation of apoptotic activity level by caspase staining showed a statistically significant difference between the 3 groups. The number of caspase stained cells was lower in the I/R + decorin group. The amount of collagen in interstitial tissue was higher in the I/R group than in the I/R + decorin group, but this difference was not statistically significant. Conclusions: We found that the TOE-Ill level the so-called initiator of fibrotic activity and apoptotic activity were low in the I/R + decorin group. Additional studies must be performed to understand the role of decorin in inhibition of TGF-beta 1 and to assess decorin's routine use in acute renal ischaemia.eninfo:eu-repo/semantics/openAccesstransforming growth factor-beta 1decoringlomerulosclerosisArticle7221121610.5114/aoms.2011.22069Q2WOS:0002909151000042-s2.0-7995635361322291758Q1